大豆의 世代短縮에 關한 硏究 Ⅰ. 種實의 登熟期間과 發芽力

Doo Chull Shin(申斗澈),Moon Kap Joo(朱門甲) 한국육종학회 1986 한국육종학회지 Vol.18 No.4

This study was conducted to confirm the germinability of unripened seeds for rapid generation advancement of soybean in field and greenhouse. Seed development and germinability were faster in greenhouse than in field, and in the early variety than in the late variety. The first germinabilities were found on the 20 days after flowering(DAF) in greenhouse materials, and on the 20 to 25 DAF in field materials. It was found that over 90 percentage of germination on 10 to 20 days before maturity of soybean in field and greenhouse, and this ripening stage was corresponded with the time when the fresh pod weight reached at the highest level. The germination ratio of the seeds dried with pod shell was the almost same level with that of the fresh seeds ripened for 10 to 15 days more. The germination ratios of the dried seeds which were harvested on 20 to 25 days before maturity in each cultivar were over 80 percentage.

大豆의 世代短縮에 關한 硏究 Ⅱ. 日長處理가 開花 및 成熟에 미치는 影響

Doo Chull Shin(申斗澈),Hyung Soo Suh(徐亨洙),Soo Kwan Lee(李壽寬) 한국육종학회 1992 한국육종학회지 Vol.24 No.4

For the four times of rapid generation advancement(RGA) of soybean in a year including two times of RGA in the greenhouse during winter, this experiment was conducted to investigate the method of two times of RGA for six months from May to October under different day lengths with natural temperature condition. For this experiment four soybean cultivars, H-25(maturity group, Ⅱ), Ishiharadaizu(Ⅱ), Milyangkong(Ⅲ-Ⅳ), Essex(Ⅴ) were planted on May 1 and August 1 in 1987 in 1/2,000a pots at the Yeongnam Crop Experiment Station(35° 29’ N, 128° 45’ E). And four different day lengths(natural, 12 hour, 10 hour and 8 hour photoperiods) were applied from unifoliate stage to flowering of soybean. The days from planting to flowering(DPF), flowering to maturity(DFM) and planting to maturity(DPM) were shortened effectively at the 10 hours day length at planting on May 1. DFM of Ishiharadaizu was the shortest by 84 days, and it of Essex was the longest by 97 days under 10 hours photoperiod among four cultivars planted on May 1. Also at planting on August 1, DFM of Ishiharadaizu was the shortest by 79 days and it of Milyangkong was the longest by 90 days under 10 hours photoperiod. Under 10 hours day length, the number of grains per plant of four cultivars were ranged from 33 to 59 at planting on May 1, and from 20 to 30 at planting on August 1. These results suggested that two times of generation could be advanced for six months from May 1 to October 31 by applying 10 hours day length from unifoliate to flowering, by harvesting immature soybean seeds before 15 to 20 days of maturity which have germinability over 80 percent.

大豆屬(Glycine spp.)의 形態的 特性에 의한 類緣關係 分析

In Youl Baek(白寅烈),Doo Chull Shin(申斗澈),Hyun Tae Kim(金賢泰),Hyung Soo Suh(徐亨洙),Yong Ho Kwack(郭龍鎬),Yun Jin Oh(吳潤鎭) 한국육종학회 1997 한국육종학회지 Vol.29 No.2

Discovery and transfer of useful characters of wild species into the elite lines may facilitate the development of crop varieties with broad genetic bases. In this study, morphological relationships among fourteen Glycine species was analyzed on basis of twenty-five morphological characters. The upper five principle components contracted from twenty-five morphological characters by the principle component analysis accounted for about 72% of the total variation. The subgenus Soja; G. max, G. gracilis, and G. soja were clearly seperated from the subgenus Glycine; G. arenaria, G. falcata, G. canescens, G. latifolia, G. microphylla, and G. tomentella by the scatter diagrams distributed on the plane of the first and second or the first and third principle components. The cluster analysis and morphological characteristics of G. cyrtoloba and G. curvata were very similar, but they are distinguished from tri-compound leaves at the unifoliate leaf node. Fourteen Glycine species could be classified into eight major groups at the average distance of 0.75 and fourteen sub-groups at the average distance of 0.45, that is, into Group Ⅰ: G. max; Group Ⅱ: A: G. gracilis, B: G. soja; Group Ⅲ: G. microphylla; Group Ⅳ: G. tomentella (2n=38, 40, 78, 80) ; Group Ⅴ: A: G. tabacina (2n=120), B: G. tabacina(2n =40, 80) ; Group Ⅵ: A: G. clandestine, B: G. canescens; Group Ⅶ: A: G. latifolia, B: G. tabacina (2n=40), G. latrobeana, C: G. cyrtoloba, G. curvata; and Group Ⅷ: A: G. falcata, B: G. arenaria.

국내 콩 유전자원의 Isoflavone 함량 변이

정명근,강성택,한원영,백인열,김현경,신두철,강남숙,황영선,안영남,임정대,김관수,박시형,김선림,Choung, Myoung-Gun,Kang, Sung-Taeg,Han, Won-Young,Baek, In-Youl,Kim, Hyeun-Kyeung,Shin, Doo-Chull,Kang, Nam-Suk,Hwang, Young-Sun,An, Young-Nam,Lim, 한국작물학회 2006 Korean journal of crop science Vol.51 No.suppl1

고 isoflavone 함유 콩 유전자원 선발 및 품종육성의 기초자료로 활용하기 위해 국내 콩 주요품종 43계통 및 보존 유전자원 648계통의 isoflavone 함량을 분석한 결과를 요약하면 다음과 같다. 1. 한국산 콩 주요 43품종의 평균 isoflavone 함량은 daidzein이 $484.4{\pm}223.9{\mu}g/g$, genisteinol $448.0{\pm}156.2{\mu}g/g$, glyciteinol $84.9{\pm}48.7{\mu}g/g$, total isoflavoneol $1,017.3{\pm}390.0{\mu}g/g$을 나타내었다. 2. 공시된 주요 품종의 isoflavone 함량은 석양풋콩이 $2,002{\mu}g/g$으로 가장 높은 함량을 나타내었고, 황금콩이 $277{\mu}g/g$으로 가장 낮은 함량을 나타내었다. 또한 콩 주요 43품종을 용도별로 분류하여 isoflavone 함량을 비교한 결과 풋콩 및 올콩이 $1,438{\pm}493.7{\mu}g/g$을 나타내어 조사된 용도별 콩 분류 중 가장 높은 양상을 나타내었다. 3. 국내 보존 콩 유전자원 648계통을 대상으로 isoflavone 함량을 조사한 결과 IT 180001계통이 $3,479{\mu}g/g$의 함량을 나타내어 가장 높은 양상을 나타내었고, IT 910878 계통은 $82.61{\mu}g/g$의 total isoflavone을 함유하여 가장 낮은 계통으로 조사되어 보존 유전자원간에 큰 함량변이를 나타내었다. Isoflavone in soybean is known to have the important physiological functions such as antioxidative, estrogenic, and anti-tumor activities. Therefore, isoflavone content is considered as important aspect of soybean quality. The objectives of this study are to determine the isoflavone contents of soybean germplasms including major cultivars in Korea, and to provide the basic information of isoflavone far development soybean cultivar containing high isoflavone content. Among 43 Korean major cultivated soybean samples, the total isoflavone contents were varied from $277.0{\mu}g/g\;to\;2,002.0{\mu}g/g$, and the average total isoflavone content was $1,017.3{\pm}390.0{\mu}g/g$. Total isoflavone content of Seokryangputkong $(2,002{\mu}g/g)$ was the highest among those of the other cultivated soybeans. On the other side, Hwangkeumkong $(277{\mu}g/g)$ contained lower isoflavone than other cultivars. The total isoflavone content of vegetable use or early maturity soybean cultivar $(1,438{\pm}493.7{\mu}g/g)$ showed more higher total isoflavone content than others. The daidzein, glycitein, genistein and total isoflavone contents of 648 soybean germplasms collected in Korea, were ranged $16.9{\sim}2,019.8{\mu}g/g,\;0{\sim}294.9{\mu}g/g,\;38.5\sim1,337.7{\mu}g/g,\;and\;82.6{\sim}3,479.2{\mu}g/g$, respectively, and total isoflavone content of IT 180001 line $(3,479.2{\mu}g/g)$ was the highest among those of other soybean germplasm lines.

연구소원외 연구논문초록 ( 재록 , 1996 ~ 1997 년 ) : 콩 품종별 전통된장 가공적성 연구

손영구(Young Koo Son),황종진(Jong Jin Hwang),김선림(Sun Lim Kim),유용환(Yong Hwan Ryu),신두철(Doo Chull Shin),유진영(Jin Young Yoo) 영남대학교 장류연구소 1998 영남대학교 장류연구소 연구업적집 Vol.1 No.-

수확기 강우가 콩의 종실 품질에 미치는 영향

강성택 ( Sung Taeg Kang ),이주석 ( Ju Suk Lee ),백인열 ( In Youl Baek ),한원영 ( Won Young Han ),신두철 ( Doo Chull Shin ),정명근 ( Myoung Gun Choung ) 한국콩연구회 2014 韓國콩硏究會誌 Vol.31 No.1

수확기 전후 강우는 종실수량감수 뿐 아니라 종자의 품질저하에 큰 영향을 미치며 차 년도에 종자로서 이용 하는데 큰 문제를 발생시킨다. 기상이변 등에 대응하여 안정적인 콩 생산을 위해서는 이러한 불량환경에 대한 내재해성을 갖춘 품종육성이 필요하다. 본 실험은 수확기 장기 강우에 의한 콩 종실의 품질저하 원인을 파악하고 강우에 의한 피해를 검정할 수 있는 적절한 검정방법을 탐색하여 내재해 품종육성의 기초자료로 활용하고자 실시되었다. 강우에 의한 종실의 품질변화를 살펴보기 위하여 포장상태에서 분수호수를 이용하여 인공강우 처리를 실시하고, 등숙기 강우피해에 대한 간이검정 방법을 탐색코자 비가림 하우스조건에서 인공강우를 실시하여 종자의 변화를 관찰하였다. 1. 포장 인공강우 처리에 의한 종실의 품질변화 정도는 발아율은 성숙이 진전될수록, 그리고 강우처리일수가 길어질수록 발아율 감소하는 경향이었다. 발아율과 발아세의 감소와 부패묘의 증가정도로 살펴본 성숙기 강우에 의한 종실의 피해 정도는 품종간 다소 차이가 있었고, 성숙의 진전에 따라서도 차이가 있었으며 또한 처리기간에 따라서 6일 이상의 처리시 급격한 품질 감소를 가져왔다. 2. 비가림 하우스를 이용한 성숙기 이후 인공강우 처리에 의한 종실의 외관 품질의 변화정도는 비정상립의 비율, 병해립의 비율이 증가하였고, 검정콩 1호, 다원콩, 대원콩, 새알콩, 은하콩, 일품검정콩은 6일간 처리에서부터 비정상립의 비율이 유의하게 높아졌으나 만리콩, 소백나물콩, 풍산나물콩은 12일 처리에서만 유의한 차이를 보여 종실외관 품질에서 품종간 차이를 나타내었다. 3. 인공강우 처리에 의한 종실의 발아관련 형질의 변화는 발아율, 발아세, 유묘부패율, 부패립 및 경실종자비율에서 품종간, 처리간 그리고 품종과 처리간 상호작용에서 고도의 유의성이 인정되고, 발아율은 대부분 6일 이상처리에서 발아율의 감소가 유의하게 나타났으나 발아세는 12일부터는 유의하게 감소하였으나 품종간에 차이를 나타내었다. 4. 인공강우처리에 의한 각 형질별 상관관계에서 발아율과 발아세는 부패묘 및 썩은 종자의 비율과 부의 상관을 가졌고 비건전종자 비율과 발아율은 정의상관을 가져 외관 종실의 형태관찰에 의해서도 어느 정도 종자 활력정도를 추정할 수 있으나 이들의 상관정도는 높지 않았다. Soybean (Glycine max (L.) Merr.) seed may lose its vigor depending on the field environment, which was prior to harvest, during development and maturation on the plant. This study was conducted to evaluate the field weathering effect on seed quality after physiological maturity and to achieve proper screening method with rain-shelter-greenhouse. To evaluate the rainfall effect on seed quality, the plant was subject to an artificial rainfall treatment in the field and greenhouse that was able to control rainfall and sunshine. After artificial rainfall treatment, seed vigor was decreased according to seed maturation degree and rainfall treatment duration. To screen tolerant soybean varieties, sufficient filed weathering has taken place after 12 days in the field and greenhouse rainfall treatment. In the both test, the small seeded varieties were more tolerant than large or medium type. Also, the greenhouse rainfall treatment was compared with field rainfall treatment to evaluate the availability of greenhouse test. The result from the greenhouse rainfall treatment can represent the result of field test. Overall, these result showed that a rainfall after maturity significantly decreased seed quality. And greenhouse rainfall test for 12 days can be used to screen soybean varieties with resistant to field weathering, which can be used for development of field weathering resistant soybean variety.

콩 품종별 전통된장 기공적성 연구

유진영,황종진,신두철,김선림,유용환,손영구 한국콩연구회 1997 韓國콩硏究會誌 Vol.14 No.2

This study way carried out to obtain the basic information about the effect of raw soybean materials (varieties) on the quality of Meju (fermented soybean). Twenty one soybean varieties, including 20 domestic and and imported variety as control, were tested for Deanjang processing. Agronomic characteristics, physico-chemical properties and quality of Meju were observed, and the, obtained results are as follows. Grain weight of raw material was positively correlated with the ratio of Length to width, but negatively with the ratio of seed coat to grain weight, respectively. Also erode fiber of raw material was negatively correlated with volume increasing rate during the water soaking (r=-0.503*) and glucose content (r=-0.642**), but positively with hardness after steaming (r=0.508**), respectively. Among the several free sugars of raw soybean materials, sucrose was considered as the major and fructose was traced on some varieties. Small amounts of maltose was detected in Saealkong also. Glucose and maltose were detected in even very small amount in imported variety especially. Total free sugar contents of four varieties (Saealkong, Namhaekong, Imported, Milyangkong) contained about 2.43 to 3.32%, but some varieties (Dankyungkong, Mallikong, Hwangkeumkong and Eunhakong) were below than 1%. The organic acids of the Meju was mainly consisted of phytic, citric. malic, pyrubic, succinic, lactic, formic, fumaric and pyroglutamic acide. The organic acid content of Meju was ranged from 1.32 to 2.55%, and three varieties such as Danbaegkong, Kwangankong and Duyaukong contained more than 2.3%. Maturing stages, crude-fiber, L value of raw soybean materials, ash and 100 grain weight were selected as the major variables which are affecting quality of Meju by using the stepwise forward regression method of SAS program. In the point of SAS analysis results, it could be considered that the liter maturing stages and high ash content make better quality of Meju. And low degree or content of water absorbtion rate, curde-fiber, L values and small main of raw soybean materials were positively correlated with Meju quality. The Korea domestic soybean varieties have more favourable properties for Meju than imported soybean varieties in same agronomic characteristics, physico-chemical properties and sensory scores. Among the 21 soybean varieties, 4 varieties including Danbaegkong, Sinpaldalkong 2, Duyoukong, Hwangkeumkong were considered having proper aptitude for Meju processing. Also six varieties including Danyeobkong, Mallikong, Bukwangkong, Milyangkong, Hannamkong and Eunhakong showed good Meju quality.

Structural Analysis of Anthocyanins in Black Soybeans

Choung, Myoung-Gun,Han, Won-Young,Kang, Sung-Taeg,Baek, In-Youl,Shin, Doo- Chull,Kim, Soo-Dong,Kim, Soon-Chul,Moon, Huhn-Pal,Kang, Kwang-Hee 한국콩연구회 2002 韓國콩硏究會誌 Vol.19 No.2

Experiments were conducted to find out the exact chemical structure of anthocyanins contained seed coats of black soybeans(Glycine max (L.) Merr.). The anthocyanins in seed coats of black soybean were extracted with 1% HCI-40%MeOH and the crude anthocyanin extract was purified by Shepadex LH-20 and Lichroprep RP-18 open column chromatography. Three anthocyanins were isolated, and their chemical structures were identified by spectroscopic methods(UV-Visible, LC/ES-MS, ^1H·^13C-NMR, and by TLC). The complete structures of these anthocyanins were elucidated as delphinidin-3-O-β-glucopyranoside(An.1), cyanidin-3-O-β-glucopyranoside (An.2) and petunidin-3-O-β-glucopyranoside (An. 3). Among these, petunidin-3-O-β-glucopyranoside was identified as a new anthocyanin in black soybeans. RP-HPLC with photodiode array detection was applied for the determination of anthocyanins in seed coats of black soybeans. Three anthocyanin pigments were separeted within 25 min. by using a isocratic elution(H_2O : CH_3OH : HCOOH = 75 : 20 : 5) on a TOSOH ODS-120T column. The application of RP-HPLC was used to rapidly examine the anthocyanin profile of the black soybean, and used to be and excellent method for deterimining the composition and contents of anthocyanins in black spybeans.

들깨 일장감응성과 관련된 RAPD marker의 선발

정대수,김현경,권일찬,정찬식,김병주,오기원,신두철,곽용호 한국생명과학회 2001 한국생명과학회 학술발표회 Vol.31 No.-