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Fructo-Oligosaccharide Production from Inulin Through Partial Citric or Phosphoric Acid Hydrolyses
José Domingos Fontana,Adelia Grzybowski,Marcela Tiboni,Maurı´cio Passos 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.11
Purified inulin from Dahlia tubers was partially hydrolyzed to form fructo-oligosaccharides by using citric or phosphoric acids (pH, 2.0–2.5) as mild acid catalysts. The ideal kinetic conditions to ensure a high yield of fructo-oligosaccharides relative to free fructose were a temperature range of 85°C–95°C, a hydrolysis time of 15–25 minutes, and a catalyst pH of 2.5. At the higher temperature and the longest hydrolysis time, an inversion of the product ratio occurred. Under these conditions, co-generation of hydroxymethylfurfural occurred, and it was eliminated by activated charcoal. Unlike in classic hydrolysis with hydrochloric or sulfuric acid, deionization of the actual hydrolysates was not necessary because the catalyst neutralization with common bases results in the formation of co-nutrients with alternative uses as foods or fermentation substrates. These whole hydrolysates can be advantageously added as nutraceuticals to carbonated beverages and acidic foods, such as soft drinks and yogurts.
Potential for colonization of O111:H25 atypical enteropathogenic E. coli
Marta O. Domingos,Keyde C.M. Melo,Irys Viana Neves,Cristiane M. Mota,Rita C. Ruiz,Bruna S. Melo,Raphael C. Lima,Denise S.P.Q. Horton,Monamaris M. Borges,Marcia R. Franzolin 한국미생물학회 2016 The journal of microbiology Vol.54 No.11
Using clonal phylogenetic methods, it has been demonstrated that O111:H25 atypical enteropathogenic E. coli (aEPEC) strains belong to distinct clones, suggesting the possibility that their ability to interact with different hosts and abiotic surfaces can vary from one clone to another. Accordingly, the ability of O111:H25 aEPEC strains derived from human, cat and dogs to adhere to epithelial cells has been investigated, along with their ability to interact with macrophages and to form biofilms on polystyrene, a polymer used to make biomedical devices. The results demonstrated that all the strains analyzed were able to adhere to, and to form pedestals on, epithelial cells, mechanisms used by E. coli to become strongly attached to the host. The strains also show a Localized-Adherence- Like (LAL) pattern of adhesion on HEp-2 cells, a behavior associated with acute infantile diarrhea. In addition, the O111:H25 aEPEC strains derived either from human or domestic animals were able to form long filaments, a phenomenon used by some bacteria to avoid phagocytosis. O111:H25 aEPEC strains were also encountered inside vacuoles, a characteristic described for several bacterial strains as a way of protecting themselves against the environment. They were also able to induce TNF-α release via two routes, one dependent on TLR-4 and the other dependent on binding of Type I fimbriae. These O111:H25 strains were also able to form biofilms on polystyrene. In summary the results suggest that, regardless of their source (i.e. linked to human origin or otherwise), O111:H25 aEPEC strains carry the potential to cause human disease.
Leukocyte platelet-rich fibrin in endodontic microsurgery: a report of 2 cases
Pires Mariana Domingos,Martins Jorge N. R.,Baruwa Abayomi Omokeji,Pereira Beatriz,Ginjeira António 대한치과보존학회 2022 Restorative Dentistry & Endodontics Vol.47 No.2
Endodontic microsurgery is a predictable treatment option when orthograde treatment or retreatment is unsuccessful or unfeasible. However, when there is a gross compromise of periapical bone, achievement of bone regeneration after the surgical procedure may be hampered. In such cases, the application of guided tissue regeneration principles, with adjunctive use of leukocyte platelet-rich fibrin to fill the bone defect as a bone substitute and as a membrane to cover the site, provides a cost-effective solution with the benefits of accelerated physiological healing and reduced post-surgical pain and discomfort. This case report presents 2 cases of endodontic microsurgery of the upper lateral incisors with loss of buccal cortical plate, where platelet-rich fibrin was successfully applied.
On the Number of <i>B<sub>h</sub></i>-Sets
DELLAMONICA Jr, DOMINGOS,KOHAYAKAWA, YOSHIHARU,LEE, SANG JUNE,RÖ,DL, VOJTĚ,CH,SAMOTIJ, WOJCIECH Cambridge University Press 2016 Combinatorics, probability & computing Vol.25 No.1
<P>A set <I>A</I> of positive integers is a <I>Bh-set</I> if all the sums of the form <I>a</I>1 + . . . + <I>ah</I>, with <I>a</I>1,. . .,<I>ah</I> ∈ <I>A</I> and <I>a</I>1 ⩽ . . . ⩽ <I>ah</I>, are distinct. We provide asymptotic bounds for the number of <I>Bh</I>-sets of a given cardinality contained in the interval [<I>n</I>] = {1,. . .,<I>n</I>}. As a consequence of our results, we address a problem of Cameron and Erdős (1990) in the context of <I>Bh</I>-sets. We also use these results to estimate the maximum size of a <I>Bh</I>-sets contained in a typical (random) subset of [<I>n</I>] with a given cardinality.</P>
XenServer 기반 가상화 환경에서 서비스 부하분산을 위한 자원할당 방안 연구
오수정 ( Soo-jeong Oh ),코스도밍고스 ( Domingos Nkosi ),박철근 ( Chul-geun Park ),김성기 ( Sung-ki Kim ) 한국정보처리학회 2014 한국정보처리학회 학술대회논문집 Vol.21 No.2
클라우드 가상화 환경에서 물리서버 노드 단위의 자원할당이 정적 로드밸런싱에 기반을 두고 있어 서비스 요청에 대한 효과적인 자원 할당의 문제가 대두되고 있다. 본 논문에서는 XenServer 기반 가상화 환경에서의 물리서버의 자원 사용량과 사용자 요청 트래픽을 모니터링하여, 동적 자원재할당하는 방안을 제시한다.
Analysis of Different Electric Current Frequencies in Soil Apparent Conductivity
dos Santos Fernando Ferreira Lima,de Queiroz Daniel Marçal,Valente Domingos Sárvio Magalhães,Khorsandi Farzaneh,de Moura Araújo Guilherme 한국농업기계학회 2023 바이오시스템공학 Vol.48 No.2
Purpose The electrical resistivity method is commonly used to identify areas with similar soil characteristics by measuring the soil’s apparent electrical conductivity (EC a ). However, the ideal frequency range to be used and the infl uence of the signal frequency on EC a values obtained by sensors is still uncertain. This study aimed to (1) evaluate the eff ect of electric current frequency on EC a values and (2) evaluate the correlation between EC a values obtained from diff erent frequencies and soil attributes of agronomic interest, such as soil water content and pH. EC a determinations were obtained in four experimental areas using six diff erent frequencies of electric current (1, 5, 10, 20, 30, and 40 Hz). Methods The infl uence of the electric current signal frequency on the value obtained by the sensor was analyzed by linear regressions in all experimental areas, followed by the F- test ( α = 0.05). Pearson’s correlations ( α = 0.05) were calculated to quantify the relationships between EC a and soil physical and chemical attributes, for each frequency of the electric current signal of the sensor. Results Statistical analyses indicated that the electric current frequency does not signifi cantly aff ect the measured conductivity value. According to the chi-square test ( p ≤ 0.05), the number of signifi cant correlations between EC a and soil physical and chemical attributes was homogeneous for all frequencies. The F -test indicated that the frequency of the electric current had no signifi cant eff ect on EC a in 90% of the experimental points. Conclusion It was shown that all frequencies in the range of 1 to 40 Hz can be used to correlate EC a values with chemical and physical soil attributes, such as soil water content ( r = 0.73, p < 0.05) and exchangeable calcium content ( r = 0.66, p < 0.05).
Bruna Carolina Dorm,José Alberto Paris Junior,Lucas Henrique Domingos da Silva,Lucimara Aparecida Forato,Tamires de Souza Nossa,Antonio José Felix Carvalho,Flávia Aparecida Resende,Alessandro Gandini 한국고분자학회 2024 Macromolecular Research Vol.32 No.2
The click chemistry reactions and its use in living organisms have been widely developed; however, few studies describe the safety of the reagents and products. Furan–maleimide represent one important pair of reagents for the click Diels–Alder reaction, and were chosen here to perform the minimalist click reaction with cysteine to reveal their preliminary safety features by cytotoxicity tests. The functional groups of amino acids allow them to be chemically modified, mainly for the synthesis of monomers and polymers. Here, cysteine was used in the click reaction, since it displays an SH group in its side chain, useful to perform thiol–ene reactions. Its amine group easily reacts with anhydrides, allowing it to couple with the furan derivative “furan–maleic anhydride adduct”. The mixture of the cysteine with the adduct in solution gives rise to the double simultaneous thiol–ene/amine maleimide click reactions. The thiol group and the furan double bond provide the reactive functional groups for the polymerization. The synthesis of the polymer based on cysteine–Diels–Alder adduct confirmed the condensation nature of the reaction. Preliminary safety and biocompatibility tests of this new polymer and its starting reagents were carried out, since these parameters represent one of the most important features for operating and scaling up the production. This one-step double-click reaction is interesting for application in several fields, since it has the advantage of using natural and renewable raw materials, in addition to its handling safety.