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( Dokyeong Kim ),( Sook Moon ) 한국치위생과학회 2021 치위생과학회지 Vol.21 No.4
Background: Cancer-associated fibroblasts (CAFs) are abundant in tumor microenvironments and interact with cancer cells to promote tumor proliferation in oral squamous cell carcinoma (OSCC). Cathepsin D (CTSD) is a soluble lysosomal aspartic endopeptidase involved in tumor proliferation and angiogenesis. In this preliminary study, we observed CTSD expression in OSCC and CAFs, postulating that CTSD might act as a bridge between OSCC and CAFs. Methods: Human epidermal keratinocytes (HEKs), OSCC, and immortalized human normal oral fibroblasts (hTERT-hNOFs) were used in this study. Additionally, we used hTERT-hNOFs transfected with an empty vector, WT (wild-type)-YAP (Yes-associated protein), and YAPS127A (YAP serine 127 to alanine). YAP127A hTERT-hNOFs activated fibroblasts similar to CAFs. To identify CTSD expression between OSCC and CAFs, conditioned medium (CM) was collected from each cell. Protein expression of CTSD was identified by western blotting. Results: To identify the expression of CTSD in fibroblasts stimulated by OSCC, we treated fibroblasts with CM from HEK and OSCC. Results indicated that hTERT-hNOFs with OSCC CM showed a weakly increased expression of CTSD compared to stimulation by HEK CM. This indicates that CAFs, YAPS127 hTRET-hNOFs, overexpress CTSD protein. HEK cells showed no CTSD expression, regardless of treatment with fibroblast CM, whereas OSCC highly expressed CTSD proteins compared with the CTSD expression in HEK cells. We also found that CTSD expression was unaffected by changes in transforming growth factor-β levels. Conclusion: This study proposes that CTSD might have potential as an interacting executor between OSCC and CAFs. Further studies are needed to investigate the role of CTSD in tumor and stromal cells.
Effect of the Orientation and Bending Stiffness of Nanopatterned Films on Wrinkling
Kwon, Dokyeong,Kim, Do Min,Choi, Soo Min,Suh, Hyo Seon,Kim, Yoon Young,Yoon, Hyunsik,Char, Kookheon The Polymer Society of Korea 2018 Macromolecular Research Vol.26 No.4
The production of hierarchical structures has an important role for applications such as superhydrophobic surfaces, structural colors, and energy devices. Microscale wrinkling of surfaces decorated by nanoscale patterns can be one of the most efficient methods to realize hierarchical structures. Here, we investigate wrinkling of films embedding anisotropic nanopatterned surfaces prepared by nanoimprint lithography. After transferring nanoimprinted polystyrene thin films onto stretched elastomeric substrates, we achieve microscale wrinkles by releasing the strain. We examine the anisotropic wrinkles of well-defined nanolines with various widths, heights, and spacing ratios and propose a model that considers only bending stiffness of the patterned film. We compare the approach with a model that considers inplane stiffness and confirm that our scheme matches well with that of nanopatterns with thin residual layers.
내진 성능을 갖춘 고성능 버터플라이밸브의 성능 인증을 위한 구조 및 유동해석
나도경(Dokyeong Na),김상훈(Sanghoon Kim),김경현(Kyeoghyeon Kim),박천(Cheon Park),도재혁(Jaehyeok Doh),임승완(Seungwan Lim) 한국신뢰성학회 2021 신뢰성응용연구 Vol.21 No.4
Purpose: Generally, the operating period of existing butterfly valves is 3 to 6 months, with continuous maintenance and replacement. It is necessary to develop a butterfly valve with high performance that increases the facility"s efficiency and water treatment capacity and ensures reliability that it can be used even in an adverse vibrating environment. Methods: This study investigates the structural safety of the new valve with the conventional valve through structural analysis. Furthermore, the cavitation occurrence and flowability are investigated based on flow analysis according to the valve"s opening rate. Results: When comparing the existing model with the new model by performing structural and flow analysis, it was found that the structural stability, improved flowability, and the degree of cavitation were reduced effectively. Conclusion: Based on these results, applying the proposed design can efficiently reduce the overseas dependence of the industry of domestic butterfly valves and can also be applied for other developing related products and industries.