BAM(Brewster Angle Microscope)으로 관측한 Langmuir막의 상전이에 따른 분자 배향에 관한 연구

조환제,강도원,박태곤 창원대학교 산업기술연구소 2001 産技硏論文集 Vol.15 No.-

In this study, we used Brewster-Angle Microscope(BAM) to study on the molecular orientation of monolayer on the water surface. The domains by behaviors of molecules on three different subphase have been observed. Reproducible π-A isotherm have been obtained only on information about phase transition by molecular area. BAM facilitates the observation of morphology by optical anisotropy and thickness in monolayer and multilayers as BAM is shown to be sensitive to anisotropy of film. Also, the coalescence of domains leads to large regions of uniform tilt-azimuthal direction. Every transition was found by BAM technique, either as a dramatic change in degree of contrast or as a sudden alteration of molecular action and π-A isotherm.

Irbesartan과 Lercanidipine의 병용요법이 고혈압 및 혈관재형성에 미치는 효과

이도형, 임태완, 조은지, 박현수, 정상혁, 한주희, 병창선 충남대학교 약학대학 의약품개발연구소 2017 藥學論文集 Vol.32 No.-

Hypertension is one of the major risk factor for cardiovascular disease and can lead to or make worse many complications, heart disease, atherosclerosis and diabetes. Thus, managing blood pressure effec tively using therapeutic drugs such as angiotensin II receptor blocker (ARB) and Ca2+ channel blocker (CCB) can be prevent other complications. Therefore, we investigated the synergistic effect of these two drugs, ir besartan and lercanidipine, for antihypertension, cardioprotection and antidiabetes. Irbesartan (28.74 mg/kg, 14.37 mg/kg) or lercanidipine (1.92 mg/kg, 0.96 mg/kg) alone or combination was administered to sponta-neously hypertensive rats (SHRs) and C57BL/6 mice. A two-week treatment of combination with irbesartan and lercanidipine significantly attenuated systolic blood pressure (SBP) and mean arterial pressure (MAP) compared with monotherapy. Futhermore. the combination therapy protected myocardial ischemia reperfusion injury more effectively than monotherapy. In vascular remodeling, combination therapy significantly diminished cuff -induced neointima formation and reduced serum -indllced vascular smooth muscle cells (VSMCs) pro-liferation compared with monotherapy. However, combination therapy had no significant synergistic effect of blood glucose regulation. Taken together, present study suggest that the combination therapy of irbesartan and lercanidipine may be more effective therapeutic strategy for the treatment of hypertension and related complications, myocardial infarction and arterial restenosis than monotherapy

풍화작용에 의한 정상모발의 형태학적 변화

홍완성,장병수,임도선,박상옥,여성문 韓國電子顯微鏡學會 2000 Applied microscopy Vol.30 No.1

성인 남성의 모발에서 정상 모간과 자연상태에서 손상된 모간의 외부형태와 미세구조적 변화를 광학현미경과 주사 및 투과전자현미경으로 관찰하였다. 정상 모간은 모소피층, 모피질, 모수질 등의 3부분으로 구성되어 있었다. 모소피층은 5-7개의 모소피세포들이 중첩되어 모피질과 모수질을 둘러싸고 있고, 길게 신장되어 모피질을 둘러싸고 있는 소피세포의 두께는 약 0.4㎛로 확인되었다. 또한, 이들 세포사이에는 약 25㎛의 세포사이 막복합체가 관찰되었다. 모피질은 잘 발달되어 각질화세포(cornified cell)들로 치밀하게 채워져 있었고, 각질화세포는 직경 약 0.5-0.8㎛인 구형 또는 타원형외 macrofibril로 구성되어 있었으며, 이들은 약 8nm 두께의 가는 microfibri이 동심원상의 규칙적인 형태로 배열되어 있었다. 그리고 macrofibril 사이에는 약 0.5㎛크기의 직경을 가진 구형의 melanin과립들이 고르게 산재되어 관찰되었다. 모수질은 공기가 채워져 있었고, 직경이 약 16㎛의 구형의 형태로 모간의 전체 직경에 1/5 정도를 차지하고 있었다. 풍화에 의해 손상된 모간은 모소피층이 박리되어 있었으며, 모피질세포의 세포질도 손상되어 melanin과립들이 표면에 노출되어 있는 것이 확인되었다. 조직표본상에서 모소피층의 박리과정은 모소피 사이에 공포가 형성되고, 이어서 모소피의 가장자리가 떨어져 나가고, 박리되지 않은 부위는 절단되었다. 모소피가 탈락되어 모피질이 노출된 부위에서는 각질화세포내에 존재하는 macrofibri이 각각 분리되고, 인접원 macrofibril 사이에 공기가 채워지게 되어 오간의 풍화과정은 더욱 촉진되었다. 그리고 노출된 macrofibril 사이에서 탈락 직전의 melanin 과립들이 산재되어 관찰되었다. The morphological changes in normal and weathering hair shafts of the human scalp were investigated by using the transmission and scanning electron microscopes. The hair shaft composed of cuticular layer, cortex and medulla. The surface of normal hairs are smooth and covered by imbricated cuticular scales. The cuticular layer consists of five to seven cuticle cells. These cells, which are flat and thin, measuring about 100 ㎛ long and 0.4 ㎛ thick, appears intercellular membrane complex in diameter 25 nm. The cortex composed of melanin granules and cornified cells, which multicomponent concentric microfibrils in diameter about 8 nm give rise to macrofibrils in diameter 0.5 ㎛ to 0.8 ㎛ encased in limiting membrane. The melanin granules are spherical shaped about 0.5 ㎛ in size and scattered between macrofibrils. The medulla in the normal hairs are 16 ㎛ in diameter centrally region of cortex. Normal hair shafts undergo progressive degenerative changes due to a variety of environmental insults. In the initial weathering process of hair, the cuticular scales became irregularly raised and broken, and then cuticle cells formed cytoplasmic vacuolation, following dissociated intercellular membrane complex, ultimately entirely lost and nuded cortex. Occasionally, transverse fissures were seen at hair shafts indicating that the hairs were deteriorated Complete removal of the cuticular layer in the heavily damaged cortex portions appeared splitting of the cortical cell into its macrofibrils and scattering of melanin granules.

인터넷 쇼핑몰에서의 소비자 구매결정속성에 대한 실증적 연구

계도원,김규완 동국대학교 경제경영연구원 1998 經濟經營硏究 Vol.22 No.1

To set up the strategy of marketing, the characters of important concerns that customers are willing to purchase some product or commodities must be analyzed and recognized in advance. Therefore, the purpose of this study is to analyze and figure out the factors of important concerns for customers to purchase some product(general product, specified planned product) in the shopping mall through Internet shopping mall directly bargained between company and customers among the electronic market. Based on the previous studies, the selection of the variables of purchasing decision attribute in the Internet shopping mall is largely divided with four categories of concerned variables. In this study the variables of purchasing decision attribute are followed. 1) The physical value of product 2) The theoretical value of product 3) The capabilities of purchasing execution in the shopping mall 4) The exchange method of product and its payment The results of this study show that customers to use the shopping mall consider in general about importantly all of four categories described above. However, this study determines that the companies to manage the shopping mall coldn't serve satisfactorily customers for 1) and 3) variables. One of the most important attribute for the case of general product in shown by only the exchange method of product and its payment. In contrast, the most important attribute of the case fo specified planned product are demonstrated by the theoretical value of product, the exchange method of product and its payment. But for the specified planned product, customers didn't receive yet satisfactorily their service for the exchange method of product and its payment. Therefore, to set up the strategy of marketing in the Internet shopping mall, the versatile methods of exchange product and its payment must be developed and also the better information of importantly concerned attribute for various product(general product, specified planned product) are provided in time. For the other variables of customer's concerns, the company should make lots of efforts support customers with satisfied service.

호주, 말레이시아, 싱가폴의 환경운동

구도완,정수복 서울대학교 사회과학연구원 1998 한국사회과학 Vol.20 No.2

불응성 자가면역질환에서의 자가조혈모세포이식

민도준,양동원,민창기,김완욱,이상헌,박성환,김동욱,이종욱,조철수,민우성,김범생,김호연,김춘추 대한조혈모세포이식학회 2001 대한조혈모세포이식학회지 Vol.6 No.1

배경: 기존의 치료에 불응하고 예후가 불량한 자가면역질환 환자들에게 최근 고용량 면역억제 및 조혈모 세포이식이 새로운 치료방법으로 대두되고 있다. 저자들은 다발성 경화증(multiple sclerosis, MS) 및 류마티스 관절염(rheumatoid arthritis, RA) 등 2명의 자가면역질환 환자들에서 자가조혈모세포 이식을 시행하였다. 방법: 말초혈액 조혈모세포 가동화를 위하여 cyclophosphamide (4 g/㎡) 및 granulocyte colony stimulating factor (10 g/kg/day)를 투여하였고, CD34+ 세포를 분리·채집 하였다, 이식 전처치로 MS 환자에서 BEAM 및 antihymocyte globulin (ATG) (3.75 mg/kg), RA 환자에서 fludarabine (180 mg/㎡), ATG (10 mg/kg)와 busulfan (8 mg/kg)을 투여하였다. 결과: 호중구 수가 500/㎕ 이상으로 회복되는 기간은 MS 환자에서 9일, RA 환자에서 15일이었다. 혈소판이 20.000/㎕ 이상으로 회복되는 가간은 RA 환자에서 9일 이었고, MS 환자에서는 혈소판 감소증이 발생하지 않았다. 비혈액학적 독성으로 MS 환자에서 WHO 1도의 오심 및 점막염이 관찰되었다. MS 환자는 이식 6개월 후까지 시력감소가 남아있었으나, 이식전에 관찰되던 감각이상 및 운동장애 등의 신경학적 이상 소견은 더 이상 관찰되지 않았다. RA 환자는 이식 1개월 후 관절 증상 및 검사소견의 호전을 보였다. 결론: 불응성 자가면역질환 환자에서 고용량 면역억제 및 조혈모세포이식은 적은 독성으로 높은 치료효과를 기대할수 있으며, 향후 이 시술의 임상적 의의를 규명하기 위하여 전향적이고 장기적인 연구가 필요할 것으로 사료된다. Background: High-dose immunosuppressive therapy followed by autologous hemathpoietic stem cell transplantation (HSCT) has been proposed as a new approach to treat severe, refractory autoimmune diseases. We describe two patients with refractory autoimmune diseases (one multiple sclerosis 〔MS〕and one rheumatoid arthritis〔RA〕) who underwent T-cell-depleted autologous peripheral bleed stem cell transplantation for the first time in Korea. Methods: We mobilized autologous stem cells with cyclophisphamide (4 g/㎡) and granulocyte colony-stimulating factor (10 ㎍/kg/day). Stem cells were enriched ex vivo using CD34-positive immunoselection and reinfused after high-dose chemotherapy with BEAM and antithymocyte globulin (ATG) (3.75 mg/kg) in MS, or fludarabine (180 mg/㎡), ATG (10 mg/kg) and busulfan (8 mg/kg) in RA. Results: The engraftment with an absolute nerutrophil count greater than 500㎕ occurred on day 9 in MS and 15 in RA, respectively. The time to nontransfused platelet count greater than 2.000/㎕ was 9 day in RA. MS patient did not show ant episode of thrombocytopenia. Regimen-related non-hematopoietic toxicity was minimal. For 6 months since HSCT, them patient with MS had been free from previously existed sensory and motor abnormalities except decreased visual acuity. Then patient with RA and only one tender joint and two mildly swollen joints with improvement in laboratory parameters at one month after HSCT. Conclusion: These results underscore the feasibility and potential efficacy of intensive immunosuppression followed by autologous HSCT for treatment of intractable autoimmune diseases. The durability of remission, however, remains to be clarified.

潛血試驗에 관한 硏究 第Ⅰ報 : Aminopyrine法 Aminopyrine Methode

李琬夏,柳柄泰,都京三 成均館大學校 1978 論文集 Vol.25 No.-

In studies of the aminopyrine-alcohol-buffer solution on the occult blood test using the filter paper method, it is found that the experimental optimum conditions are 4% aminopyrine. 10% of H_2O_2, pH5 of the Walpole buffe、r solution Toyo filter paper No.2, 1 min of standing time, and the 7 : 3 ratio of aminopyrine to H_2O_2 (V/V) As the sensitivity of this method agrees to that of Hematest, it is considered that this method can be used as a clinical test in occult blood.

펜아실 벤젠슬포네이트와 벤질아민의 친핵성 치환반응에 관한 연구

홍성완,박도순 又石大學校 1992 論文集 Vol.14 No.-

The nucleophilic substitution reaction of phenacyl benzenesulfonates(PBS) with benzylamine(BA) in methanol have been studied by conductometric method. The transition state structure for the reaction is investigated using various selectivity parameters, especially with cross interaction constants, ρ_ij β_ij The magnitudes of ρ_ij indicated that in the transition state bond breaking had progressed very little, but bond formation was substantial. The trends in reactivity were similar to aniline series, but the TS was found to be somewhat looser than that for aniline series. It was found that reactivity-selectivity principle(RSP) and Bell-Evans-Polanyi principle (BEP) hold within the BA series and hence constituted a good example of a thermodynamically controlled reaction series but are violated between two different reaction series with BA and aniline.

1990년대 전문 환경운동조직의 특성

구도완 울산대학교 사회과학연구소 2000 사회과학논집 Vol.10 No.1

이 글은 1990년대 한국의 전문환경운동조직을 경험적으로 분석하고 '제도화'라는 관점에서 그 특성을 살펴보는 데 목적이 있다. 전문 환경운동조직 가운데 대표적인 세 단체, 즉 환경운동연합, 녹색연합, 환경정의시민연대(경실련 환경개발센타)의 역사와 조직, 이념, 참여자, 재정, 주요활동을 분석했다. 전문 환경운동조직은 조직이 커졌고, 재정이 튼튼해졌으며, 사회적 영향력도 매우 커졌다. 이념은 1980년대의 민주화운동에 비해 현실주의적으로 변화했고 생태주의적 요소가 강해졌다. 이러한 전문 환경운동조직의 성장은 제도화 과정을 통해 이루어졌다. 제도화의 정도를 판별하기 위한 다섯 가지 요인 즉 조직의 일상화, 합법적 자원동원, 조직목표의 체제순응 정도, 인적 자원의 포섭, 다른 조직과의 연결망 등을 분석한 결과, 세 단체는 모두 제도화 과정을 밟아왔다. 이 가운데 경실련(환경개발센타)이 가장 제도화되었고, 다음으로 녹색연합, 환경운동연합의 순으로 제도화가 이루어진 것으로 분석되었다. 1990년대 전문 환경운동조직들은 제도화 과정을 통해 얻은 사회적 권력을 바탕으로 제도 안팎의 저항을 조직하는 한국사회의 새로운 사회운동 모델을 창조했다. This paper aims to analyze the environmental movement organizations during the 1990s period in South Korea, in terms of "institutionalization". We analyzed histories, organizations, ideology, participants, and fundings of the 3 major environmental movement oranizatiosn, Korean Federation for Environmental Movement(KFEM), Green Korea, and Environment and Development Center in Citizens 'Coalition for Environmental Justice(CCEJ). In the 1990s, the 3 organizations got greater members, were successful in fund raising, and became very influential in environmental politics. Radical ideology changed to realistic, modest and ecological. We found that the environmental movement organizations became influential in the process of "institutionalization". We analyzed the 5 factors measuring "institutionalization" that are routinization of organizations, legal resource mobilization, modest organization programme, co-optation, network with other organizations. We found that CCEJ was the most institutionalized and KFEM was less institutionalized. However, environmental movement organizations were not totally instituionalized. They made environmental issues on the social, political agenda and created new type of environmental politics of civil society in South Korea.

자가부식형 상아질접착제와 레진시멘트와의 적합성에 관한 연구

김도완,박상진,최경규 大韓齒科保存學會 2005 Restorative Dentistry & Endodontics Vol.30 No.6

본 연구는 상아질 접착제와 레진 시멘트의 적합성 여부를 알아보고자 시행하였다. 발거한 제3대구치의 상아질 표면을 노출시킨 후 Tescera ATL 복합레진 시편을 상아질 접착제 [All Bond 2 (Bisco), Clearfil SE Bond (Kuraray), Adper Prompt L-POP (3M), One-Up Bond F (Tokuyama)]와 레진시멘트 [Choice (Bisco), Panavia F (Kuraray), RelyX ARC (3M), Bistite II DC (Tokuyama)]로 접착하고 미세인장 결합강도 및 주사전자 현미경 관찰을 시행한 결론은 다음과 같다. 1. Clearfil SE Bond와 All-Bond 2가 Prompt L-Pop과 One-Up Bond F 보다 높은 미세인장 결합강도를 보였다(p < 0.05). 2. Clearfil SE Bond와 All-Bond2 사용시 1-step 상아질 접착제보다 혼성층이 두껍고 레진 tag가 길었다. This study was performed to investigate the compatibility between 4 dentin adhesives and 4 resin luting cements. Dentin adhesives used in this study were All-Bond 2 (Bisco Inc., Schaumbrug, IL, USA), Clearfil SE-Bond (Kuraray Medical Inc, Osaka, Japan), Prompt L-Pop (3M Dental Products, St. Paul, MN, USA), One-Up Bond F (Tokuyama corp., Tokyo, Japan). Resin luting cements used in this study were Choice (Bisco Inc., Schaumbrug, IL, USA), Panavia F (Kuraray Medical Inc, Osaka, Japan), RelyX ARC (3M Dental Products, St. Paul, MN, USA), Bistite II DC (Tokuyama corp., Tokyo, Japan). Combination of each dentin adhesive and corresponding resin cement was made to 16 experimental groups. Flat dentin surfaces was created on mid-coronal dentin of extracted mandibular third molars, then dentin surface was polished with 320-grit silicon carbide abrasive papers. Indirect resin composite block (Tescera, Bisco) was fabricated. Its surface for bonding to tooth was polished with silicon carbide abrasive papers. Each dentin adhesive was treated on tooth surface and resin composite overlay were luted with each resin cement. Each bonded specimen was poured in epoxy resin and sectioned occluso-gingivally into 1.0 ㎜ thick slab, then further sectioned into 1.0 × 1.0 ㎟ composite-dentin beams. Microtensile bond strength was tested at a crosshead speed of 1.0 ㎜/min. The data were analysed by one-way ANOVA and Duncan's multiple comparison tests. The results of this study were as follows; 2-step self-etching dentin adhesive which has additional bonding resin is more compatible than 1-step self-etching dentin adhesive.