저산소에 노출된 넙치(Paralichthys olivaceus)의 호흡대사와 혈액의 화학적 변화

한지도 ( Ji Do Han ),김흥윤 ( Heung Yun Kim ) 한국수산과학회 2016 한국수산과학회지 Vol.49 No.1

This experiment investigated changes in metabolic rate (MO2), critical oxygen saturation (Scrit), and blood chemistry of olive flounder Paralichthys olivaceus exposed to progressive hypoxia and returned to normoxic water at 20°C. The normoxic standard metabolic rate (SMR) and routine metabolic rate (RMR) were 69.5-83.9 and 70.2-156.4 mg O2 kg-1h-1, respectively based on fish weight. Scrit was 31.0% dissolved oxygen (DO) at 20°C. After returning the fish to 70% DO following exposure to hypoxia (20% DO), MO2 increased two-fold compared to the normoxic SMR and then decreased into the range of the RMR with time. Blood PO2 and plasma lactate decreased significantly after exposure to hypoxia (20% DO) and then increased as ambient oxygen saturation decreased. Cortisol levels increased as ambient oxygen saturation decreased, but the levels decreased rapidly in the range of the normoxic control when the fish were returned to ambient water with 70% DO. Plasma glucose levels increased when the fish were returned to normoxic water after exposure to a progressively more hypoxic condition.

북한의 주체사상과 사회변화

도흥열 경북대학교 평화문제연구소 1989 평화연구 Vol.14 No.-

입방정 집합조직을 가진 고순도 알루미늄의 염산용액 중 전해에칭 연구

서도수,김흥식 울산대학교 2002 공학연구논문집 Vol.33 No.2

입방정 (100)<001> 집합조직을 가진 고순도 알루미늄 포일을 묽은 염산에 에칭함으로서 직경 1~2㎛ 인 관통형 터널피트를 단위면적당 약 10^{7}개 이상 형성하여 알루미늄 전해캐퍼씨터의 정전용량을 극대화시키는 연구를 수행하였다. 고순도 알루미늄 포일을 이용하여 전류밀도 및 통전량의 변화에 따라 포일에 형성된 에치피트의 관찰과 정전용량의 변화를 측정하여 내전압 265V_{f}에서는 전류밀도가 150mA/cm²에서 최대의 정전용량값(1.70μF/cm²)을 얻었으며, 내전압 540V_{f}에서는 전류밀도가 200mA/cm²에서 정전용량이 최대값(0.60μF/cm²)이었고, 내전압 600V_{f}에서는 전류밀도가 200mA/cm²에서 최대의 정전용량의 값(0.58μF/cm²)을 얻었다. 또한, 내전압 265V_{f}에서는 통전량 60 Coulomb/cm², 540V_{f}및 600V_{f}에서 68 Coulomb/cm²이 최적 전해에칭 조건이었다. A Study on capacitance gain of high voltage aluminium electrolytic capacitor has been conducted by etching cubic textured aluminium foil in hydrochloric acid. Capacitance obtained by etching and anodizing of aluminium of was decreased with the increase of anodizing voltage. Maximum capacitance obtained by anodizing at 260V was 1.70μF/cm², however, maximum capacitance obtained by anodizing at 600V was 0.58μF/cm². Optimum electrochemical etching conditions for obtaining maximum capacitance was varied according to anodizing voltage of etched aluminium foil.

용융 알루미늄 도금 강재의 부식 특성

서도수,송용화,김흥식 울산대학교 2002 공학연구논문집 Vol.33 No.2

용응 알루미늄 도금방법으로는 피도금물을 산세에 의해 피도금물 표면의 유지 또는 산화물을 완전히 제거한 것을 용응알루미늄속에서 일정시간 동안 침지시키면 철강표면에 철과 알루미늄과의 합금층이 생기며 그 합금층 위에 알루미늄이 부착 도금되는 것이다. 본 연구에서는 용음알루미늄 도금강재의 부식 특성을 평가하기 위해 염수분무시험을 실시하여 표면을 관찰하므로서 해수에서 용응알루미늄 도금 강재를 적용할 경우 내부식성을 평가하였다.

악성 거대 난소 점액성 종양 1예

신형도,박형진,김홍곤,한원철 圓光大學校 醫科學硏究所 1999 圓光醫科學 Vol.15 No.1

Mucinous tumor are cystic tumors with loculus lined with mucin-secreting epithelium constitute approximately 8-10% of primary epithelial ovarian tumors. Of all mucinous neoplasms, 75-85% are benign. It is often reach very large size particularly with the benign counterpart being reported on occasion to have exceed 100 lbs. Malignancy develops in 5-10% of benign mucinous cysts. About 15-20% of neoplasms are bilateral. We experienced a huge ovarian mucinous tumor of 155 ㎏. measured 42 × 30 × 28 ㎝ in size, and the volume of cystic contents was about 10 L in a nulligravida 36-year-old woman. Pathologic diagnosis was moderately differentiated mucinous cystadenocarcinoma of right ovary. So, we presented with a brief review of literatures.

10Gbps 2:1 멀티플렉서 설계

이상흥,김도우,구경완 永同大學校 2000 硏究論叢 Vol.6 No.1

광통신 시스템의 송신부에 있어서, 시분할 멀티플렉서는 여러 병렬스트림(stream)들을 높은 비트율을 갖는 하나의 직렬스트림으로 결합한다. 본 논문에서는 2채널(2:1) 시분할 멀티플렉서를 2×8㎛2 크기의 에미터를 갖는 SiGe HBT를 사용하여 설계하였다. 설계된 회로의 동작속도는 10Gbps, 20%∼80%간의 천이시간인 상승시간과 하강시간은 각각 34ps와 35ps이며 전력소모는 0.86W이었다. In the transmitter of optical fiber transmission systems, a time-division multiplexer combines several parallel data streams into a single data stream with a high bit rate. In this paper, we design a 2:1 (2-channels) time-division multiplexer using SiGe HBT with emitter size of 2x8um2. The operation speed is 10Gbps, the rise and fall times of 20-80% are 34ps and 35ps, respectively and the dissipation of power is 0.86W.

스테비아(Stevia rebaudiana Bertoni)에 있어서 收穫時期 및 方法이 收量에 미치는 影響

金興培,吳現道 濟州大學校 亞熱帶農業硏究所 1985 亞熱帶農業硏究 Vol.2 No.-

스테비아의 收穫時期 및 方法이 生育과 收量에 미찌는 影響을 究明하기 위하여 調査한 結果를 要約하면 다음과 같다. 草長의 伸長은 幼苗期에 緩慢하게 進行되다가 그 後 발뇌기까지 氣溫의 上昇과 더불어 急激하게 伸長하였고 그 以後에는 伸長速度가 低調하였으며 分枝數와 葉數는 8月中~下旬에 增加現象이 뚜렷하였다. 乾物重의 增加는 生體重의 增加와 비슷한 傾向을 보였고 乾根重의 增加도 地上部 乾物重과 比例的으로 增加하였다. 生育 時期別 全 乾物重에 대한 植物體 各 部分의 乾物衆의 比率은 幼苗期에는 잎이 월등히 높고 生育이 進展됨에 따라 莖枝의 比率이 漸次 增加하여 生育 後期에는 오히려 잎보다 높아졌다. 收穫時 刈取部位가 높을 수록 枯死率이 增加하였으며 晩刈區는 早刈區에 比해서 枯死率이 높았고 適定 刈取部位는 10∼15cm였다. 收穫回數는 年2回(1回 : 8月 5日 2回 : 9月20日) 하는 것이 收量도 많았고 安全한 收穫 方法으로 認定되었다. This experiment was carried out to evaluate effects of harvesting time and height on growth and yield of stevia in order to establish its cultural pracctices in cheju-do. The growth of plant height showed three patterns: slow increase at the early growth stage, rapid increase at the bud formation stage and decrease after the bud formation. The number of branhches and leaves greatly increased during the period from middle to late August. The leaves contributed to increase int the total dry weight at the early stage, but stems and branches contributed to increase in the total dry weight as growth stage progressed. The percentage of dead plants was decreased as the cutting height from ground at the first harvesting was increased and the optimum cutting height was 10 to 15cm. The percentage of dead plants was higher in the late cutting than in theearly cutting. The highest yield was obtained from the treatment of two times harvesting on 5th August and 20th September.

갑상선 세포에서 전사보조활성인자인 CBP와 CIITA의 인터페론-감마 활성 부위에 대한 조절 : CBP and CIITA on GAS(Interferon- γ Activated Site)-Mediated Transcription in Thyroid Cells

채수흥,홍우정,김군순,조영석,김도희,김현진,구본정,송민호,김영건,노흥규 충남대학교 의과대학 지역사회의학연구소 2001 충남의대잡지 Vol.28 No.1

Background : In the previous stuides, we identified that the interferon-r activated sequence (GAS) in the 5-flanking region of rat ICAM-1 gene is major element for interferon-r-inducible expression of the gene in rat thyroid cells, FRTL-5. We here, investigated the role of transcriptional coactivators, CBP(CREB binding protein) and CⅡTA (class Ⅱ transactivator) in the modulation of the activity of GAS which could interacts with signal transducers and activators of transcription-1 and 3 (STAT1 and STAT3). Methods : The expression of CBP RNA and protein were quantitated in FRTL-5 after stimulation with interferon-r (IFN-r), thyroid stimulating hormone (TSH), forskolin and methimazole. Direct association of CBP with STAT were analyzed by immunoprecipitation. The transcriptional roles of CBP and CⅡTA in the regulation of GAS were assessed by the cotransfection with their expression vectors with reporters; 5-deletion constructs of rat ICAM-1 promoter or 8xGAS-luc constructs, into FRTL-5 thyroid cells. Results : The level of CBP RNA and protein were not changed by the treatment with TSH, IFN-r, forskolin and methimazole in FRTL-5, FRT and BRL liver cells. The CBP could be directly associated with STAT1. Furthermore, the overexpression of CBP significantly increases the both promoter activities; rat ICAM-1 gene promoter which has GAS element and 8xGAS-luc cassette constructs. However, the cotransfection of CⅡTA decreased the constitutive and CBP-mediated transactivation of rat ICAM-1 promoter and 8xGAS-luc cassette constructs. Conclusion : We identified that the two tanscriptional coactivators; CBP and CⅡTA has differential roles in the regulation of transcriptional activity of GAS drived promoter. CBP increases the GAS activity through the direct binding with STAT1, but CⅡTA inhibited the CBP-mediated transactivation of GAS activity.

水稻의 胚 培養時 器官 및 體細胞 胚形成에 關與하는 蛋白質과 酵素에 關한 硏究

金興培,李都榮 동국대학교 농림과학연구소 1988 農林科學 論文集 Vol.12 No.-

This study was carried out to investigate several factors affecting embryo-genesis and organogenesis in vitro culture of rice embryo and the results indicated that Callus induction was the highest on LS agar medium containing 2.5㎎/L of 2.4-D and MS agar medium containing 2㎎/L of 2.4-D without vitamine. Zymograms of seed proteins determined by SDS-PAGE showed that they were quite similar, but only two major band were different between Gihobyeo and Milyang 23. One had a molecular weight of 40KD in Milyang 23 and another had a molecular weight of 55kD in Gihobyeo. After two weeks of culture, not-well induced Callus showed a distinct protein band having molecular weight of 20kD as compared to well induced Callus Calli on N60 and MSo media without auxin seemed to be embryogenic and showed similar batterns to seed proteins. Milyang 23 was higher in enzyme activity than Gihobyeo. Addition of abscisic acid on media resulted in increasing peroxidase activity and addition of BA increased on the malate dehydrogenase activity. A additional esterase isozyme band of pI 4.0 occurred at an early stage of rice Callus on medium with auxin. But Calli cultured on N60 and MSo media without auxin had similar esterase isozyme band patterns to seeds and Calli were embryogenic. Best plantlet formation was observed on medium with combination of 0.2㎎/L of IAA and 4㎎/L of BA. Embryogenic and organogenic Callus had characteristic protein bands of molecular weight of 40kD and 50kD determined by SDS-PAGE.

양성 갑상선 결절에서의 무수 알코올 치료 효과

김도희,나소영,이효진,홍우정,김군순,조영석,구본정,송민호,김영건,노흥규 충남대학교 의과대학 의학연구소 2002 충남의대잡지 Vol.29 No.1

목적 : 양성 갑상선 결절 환자에서의 PEI의 치료효과 및 부작용을 알아보고자 하였다. 방법 : 양성 갑상선 결절 환자 88명을 대상으로 초음파 소견에 따라 낭종성 결절, 복합성 결절, 고형성 결절 등 세 그룹으로 분류후 전체 및 각각에 대한 PEI의 치료효과 및 부작용을 조사하였다. 결과 : 양성 갑상선 결절 환자 88명을 PEI로 치료후 결절의 크기가 50%이상 감소한 경우는 전체 환자중 77.2%이었고 각 결절별로 살펴보면 고형성 결절에서 52.6%, 복합성 결절에서 72.4%, 낭종성 결절은 92.5%이었다. 부작용은 동통, 결절내 출혈, 감염, 결절의 누출등이 있었으나 대부분 무증상이었다. 결론 : PEI는 양성 갑상선 결절 환자증 적응증을 잘 살펴 고려해 볼 수 있는 치료법이라 생각되며 특히 낭종성 결절 환자에서 주사기에 의한 흡인후에 재발한 경우 일차적인 치료로 PEI를 고려해 볼 수 있겠다. Ultrasonography(US)-guided percutaneous ethanol injection (PEI) has been performed for the treatment of autonomous functioning thyroid nodules, thyroid cysts, & cold thyroid nodules. The author studied the efficacy of PEI in the treatment of benign cold thyroid nodules both solid and cystic (complex & pure). Eighty-eight euthyroid outpatients (age:44.2±13.9years, 75 women and 13 men) with benign cold thyroid nodules or simple thyroid cysts (69 with a single nodule and 19 with a prominent nodule within multinodular goiter) were includied in this study. After PEI, each subtype of thyroid nodules was divided into three groups in according to volume reduction complete response (90% or more of volume reduction), partial response (50% or more of reducton but below 90%), and failure (below 50% or increase in size) group. Overall response rates of PEI for benign thyroid nodules were complete 45(51.1%), partial 23(26.1%), failure 20(22.7%). In 20 cold solid nodules, complete response was observed in 6(30.0%), partial response in 5(25.0%), and failure in 9(45.0%). In 28 complex cysts, complete response was observed in 8(28.6%), partial response in 12 (42.9%), and failure in 8(28.6%). In 40 pure cysts, complete response was observed in 31(77.5%), partial response in 6 (15.0%), and failure in 3 (7.5%). Side effects comprised pain 8 (9.2%), intranodular bleeding 13 (14.6%), extracapsular leakage 1(1.1%), infection 1 (1.1%), increased size l(1.1%), and none 64(72.7%). PEI may prove a safe & effective tool for the therapy of cold thyroid nodules in selected cases and may be the treatment of choice of recurrent thyroid cysts. However, further investigation involving a larger number of patients and a longer follow-up is needed.