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Effect of Vascular Endothelial Growth Factor on Porcine In Vitro Maturation
Dibyendu Biswas,현상환 사단법인 한국동물생명공학회 2007 한국동물생명공학회지 Vol.22 No.4
This study was performed to investigate the effect of VEGF on in vitro maturation of porcine oocytes. The base medium for IVM, TCM-199 was supplemented with 0.6 mM cysteine, 0.91 mM pyruvate, 10 ng/ml epidermal growth factor, 75 μg/ ml kenamycin, 1 μg/ml insulin and 10% (V/V) porcine follicular fluid (pFF) as a Group A; Group B was consists of Group A plus 5 ng/ml VEGF; Group C was consists of replacement of pFF by 10% PVA and Group D: was consists of Group C plus 5 ng/ml VEGF. 1. The maturation rate was significantly higher (p<0.05) in control and VEGF+pFF group than other two groups (76.1± 9.6, 78.9±6.0 vs 60.4±14.2 and 58.3±14.3, respectively). 2. Addition of VEGF without pFF showed a negative effect on oocyte maturation and about 58.26% oocytes were reached to M-Ⅱ stage. 3. In the parthenogenetic development, the cleavage rate was significantly higher (p<0.05) in control and VEGF+ pFF group (73.2±1.8 and 64.6±1.1, respectively) than other groups (47.9± 1.8 and 48.3±1.7, respectively). 4. The blastocyst formation rate was significantly higher (p<0.05) in VEGF+pFF group (32.6±2.4) compared to control and other groups.5. There was no significant difference in cell numbers (inner cell mass or trophectoderm) among these groups.
Dibyendu Debnath,B. B. Khatua 한국고분자학회 2011 Macromolecular Research Vol.19 No.6
The effect of nanoclay on the thermal stability of polystyrene (PS)-poly(methyl methacrylate) (PMMA)core-shell nanocomposites morphology prepared by in situ suspension polymerization technique was investigated. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) revealed the formation of a PSPMMA core-shell structure, both in the absence and presence of the clay. Wide angle X-ray diffraction (WAXD)indicated the formation of a highly intercalated expanded clay morphology in the core-shell composite particles irrespective of the location of the clay in the nanocomposites. Thermo gravimetric analysis (TGA) indicated an improvement in the thermal stability of the core-shell nanocomposites compared to that of the neat PS-PMMA coreshell one. Differential scanning calorimetry (DSC) showed that the core-shell nanocomposite particles containing the clay had a higher glass transition (T_g) temperature than the neat PS-PMMA core-shell composites particles. On the other hand, the improvement in the thermal stability of the PS-PMMA core-shell composites was found to depend strongly on the location and loading of the clay in the core-shell nanocomposites. FTIR indicated a probable interaction between the carbonyl (>C=O) group of PMMA and hydroxyl (-OH) group of the clay.
Subcontracting, R&D and Labour Productivity : A Theoretical Explanation
DIBYENDU MAITI;SUGATA MARJIT 경제연구소 2011 Journal of Economic Development Vol.36 No.3
While a large body of researches discusses the effects of international subcontracting on firm dynamics, the present work deals with the similar issues of a domestic firm who subcontracts to the informal sector in a typical developing world. Theoretically, we develop a model that if the formal sector wage is higher than that of informal sector, the choice of informal sector subcontracting and in-house R&D investment appears to be alternative options to the firm to bypass expensive labour in the formal sector. We argue that the R&D and labour productivity in formal sector are highly influenced by the informal wage but not the formal sector one. Since the subcontracting can raise both supply and demand for informal workers due to a rise of formal sector wage, the movement of informal sector wage is uncertain and thereby, the formal sector R&D and labour-productivity are also ambiguous. Thus, countries with a vast segment of lowly-paid informal workers exhibit lowly-productive formal workers.
SUBCONTRACTING, R&D AND LABOUR PRODUCTIVITY: A THEORETICAL EXPLANATION
DIBYENDU MAITI,SUGATA MARJIT 중앙대학교 경제연구소 2011 Journal of Economic Development Vol.36 No.3
While a large body of researches discusses the effects of international subcontracting on firm dynamics, the present work deals with the similar issues of a domestic firm who subcontracts to the informal sector in a typical developing world. Theoretically, we develop a model that if the formal sector wage is higher than that of informal sector, the choice of informal sector subcontracting and in-house R&D investment appears to be alternative options to the firm to bypass expensive labour in the formal sector. We argue that the R&D and labour productivity in formal sector are highly influenced by the informal wage but not the formal sector one. Since the subcontracting can raise both supply and demand for informal workers due to a rise of formal sector wage, the movement of informal sector wage is uncertain and thereby, the formal sector R&D and labour-productivity are also ambiguous. Thus, countries with a vast segment of lowly-paid informal workers exhibit lowly-productive formal workers.
Dibyendu Talukdar 한국작물학회 2012 Journal of crop science and biotechnology Vol.15 No.4
The effect of calcium (Ca) on lentil (Lens culinaris Medic.) seedlings exposed to cadmium (Cd) stress was studied by investigating plant growth and antioxidant enzyme activities. Plants were grown for 14 days in full-strength Hoagland nutrient media supplemented with Cd concentrations of 0, 10, 20, and 40 μM, and on corresponding medium supplied with 5 mM Ca(NO3)2 prior to Cd addition. Increasing Cd led to accumulation of metal and reduced the fresh weight of the shoots more strongly than that of the roots. Cd concentrations of 20 and 40 μM were selected to study its toxic effect on seedlings. Activities of superoxide dismutase, ascorbate peroxidase, catalase, dehydroascorbate reductase, and glutathione reductase decreased at much higher magnitude in the shoots than those observed in the roots under Cd exposure. Failure of antioxidant defense in scavenging of reactive oxygen species was evidenced by abnormal rise in H2O2, resulting in enhancement of lipid peroxidation and membrane electrolyte leakage as the marks of Cd-induced oxidative stress in lentil seedlings. Ca priming in the media significantly reduced the Cd accumulation and considerably alleviated the adverse impact of Cd treatment by modulating the antioxidant enzyme activity. Mitigation of Cd-induced stress by Ca application was strongly suggested by declining levels of H2O2 and consequent lowering of oxidative damage of membrane. Consequently, this enhanced fresh mass of plant parts as the sign of Ca-mediated normal growth in Cd-treated lentil seedlings.