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Rhizobium halotolerans sp. nov., Isolated from Chloroethylenes Contaminated Soil
Diange, Eboa Adolf,Lee, Sang-Seob Springer-Verlag 2013 Current microbiology Vol.66 No.6
<P>The strain designated as AB21(T) was isolated from chloroethylenes contaminated soil. Cells are gram-negative, aerobic, non-spore-forming, and motile rods. Phylogenetic analysis based on 16S rRNA gene sequence showed that it belonged to the genus Rhizobium, and was closely related to Rhizobium sullae IS 123(T) (97.4 %), Rhizobium yanglingense SH 22623(T) (97.2 %), Rhizobium gallicum R 602sp(T) (97.1 %), Rhizobium alamii GBV 016(T) (97.0 %), and Rhizobium monogolense USDA 1844(T) (97.0 %). It showed less than 97 % identity with the remaining Rhizobium species. This novel isolate grew optimally at 25-37 C (optimum, 30 C) and pH 6-9 (optimum, pH 8.0). It grew in the presence of 0-4 % (w/v) NaCl, tolerating a 4 % (w/v) NaCl. DNA-DNA hybridization experiment shows less than 53 % binding with closely related Rhizobium. Predominant quinone is ubiquinone (Q-10). The major fatty acids were summed feature 8 (composed of C(18:1) ω7c/C(18:1) ω6c), C(19:0) cyclo ω8c, and C(16:0). The G+C molar content is 62.5 mol%. Based on the polyphasic analysis, strain AB21(T) is referred to be a novel species of the genus Rhizobium for which the name Rhizobium halotolerans sp. nov. is proposed. The type strain is AB21(T) (=KEMC 224-056(T) = JCM 17536(T)).</P>
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Design and Research on the Special System for High Efficient Manufacturing of Blisk Based on UG
Yaonan Cheng,Rui Guan,Diange Zuo,Tianqi Chen,Li Liu 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.10
As one of the most important parts of aeroengine, blisk has complex structure and its material is titanium alloy hard to machine. It results in some difficulties in modeling and NC programming. In this paper, from the perspective of application, the secondary development technology of UG/Open Grip is used to design and develop the CAD/CAM module of blisk after analyzing structure feature and milling process of blisk. Firstly, it completes parametric modeling of blisk by establishing CAD module through the optimization of the original data points in NUBRS curve fitting and node interpolation technology; secondly, the inner and outer tolerance algorithm are applied to calculate the cutting step, and the row spacing is determined based on constant scallop height. Besides, tool axis vector is defined. All of these things are done to complete the establishment of the CAM module and realize trajectory planning of semi-finish machining of the blisk. It greatly improves the efficiency of programming and machining in blisks; finally, the module of blisk designed is used to model and program in the machining experiments in five axis machining center to verify correctness and reliability of the system. The results show that this module greatly improves manufacturing efficiency of blisk.
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Meng Tianchuang,Yang Tianhong,Huang Jin,Jin Wenrui,Zhang Wei,Jia Yifan,Wan Keqian,Xiao Gang,Yang Diange,Zhong Zhihua 한국자동차공학회 2023 International journal of automotive technology Vol.24 No.2
The recent proliferation of intelligent technologies has promoted autonomous driving. The autonomous parking system has become a popular feature in autonomous driving. Hybrid A-star algorithm is a commonly used path planning algorithm for its simplicity to deploy and the good characteristics of the generated paths in the practical engineering. To further enhance the path safety and efficiency of path planning in the autonomous parking system, this paper proposes an improved hybrid A-star algorithm through the safety-enhanced design and the efficiency-enhanced design. The safety-enhanced design integrates the Voronoi field potential into the path searching stage to take more account of path safety. The efficiency-enhanced design proposes a multi-stage dynamic optimization strategy which divides the path planning into multiple stages and performs dynamic optimization in each stage. Through simulation experiments, it is verified that the proposed improved algorithm not only generates a much safer path which stays farther from the obstacles but also significantly improves the searching efficiency in terms of time and space, merely at a finite cost of pre-processing work which can also be repeatedly utilized. We hope this paper will promote relative research on path planning in autonomous parking and serve as a reference for the practical engineering.
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Sagita Diang,Kristanti Dita,Setiaboma Woro,Kumalasari Rima,Ekafitri Riyanti,Yulianti Lista Eka,Putri Devry Pramesti,Ardiansyah Raden Cecep Erwan,Desnilasari Dewi,Hariadi Hari 한국농업기계학회 2023 바이오시스템공학 Vol.48 No.3
Purpose Fermentation in the form of green coffee beans has the potential to alter the flavor and even produce new flavor profiles. However, existing coffee fermentations were still performed traditionally and used ineffective fermenter equipment. In this study, ohmic heating (OH) was first applied to the green coffee bean fermentation. The impact of ohmic heating on the fermentation of green coffee beans was examined. Methods The experiments were performed using green coffee beans, water, and a 1% of instant dry yeast (S. cerevisiae). Three voltage gradient (VG) levels were used as follows: 4, 8, and 12 V/cm. Non-OH fermentation (0 V/cm) and coffee beans without fermentation were also evaluated as controls. The observed parameters included voltage, electrical current, electrical conductivity (EC), power, total energy consumption, coffee bean temperature, environmental temperature, pH of the fermentation medium, and the fermentation index of coffee beans. Results The results showed that OH was effective in generating heat for the green coffee bean fermentation process. The set point temperature was reached faster with increasing VG. The coffee bean temperature was successfully maintained throughout the fermentation process for all VG variations. The variation in VG significantly impacted the electrical current, power, and time needed to reach the set point temperature but did not have a significant effect on total energy consumption and EC. During fermentation, the EC of the green coffee beans with instant yeast solution increased due to an increase in electrical current. The final pH value was significantly different between OH and non-OH fermentation, with OH fermentation resulting in the highest fermentation index. Conclusions The findings of this study indicate that OH treatment can significantly improve the fermentation process of green coffee beans indicated with lower pH and higher fermentation index.
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