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Contributions of Parental Lineage on the Community Development of Rice Seed Bacterial Endophytes
( Denver Walitang ),김기윤 ( Kiyoon Kim ),( Aritra Roy Choudhury ),강연경 ( Yeongyeong Kang ),사동민 ( Tongmin Sa ) 한국환경농학회 2017 한국환경농학회 학술대회집 Vol.2017 No.-
There is relatively diverse groups of bacterial endophytes inhabiting the seeds of rice that could become the dominant sources of endophytes of rice host as bacterial endophytes are transmitted and conserved in the next generations. The objective of this study is to gain insights into the contributions of parental lineage and crossbreeding on the seed bacterial endophytic communities of two pure inbred lines exploring influence of the two most important sources of plant endophytes - colonization from external sources and vertical transmission via seeds. Total genomic DNA was isolated from rice seeds and bacterial DNA was selectively amplified by PCR. The diversity of endophytic bacteria was studied through Terminal-Restriction Fragment Length Polymorphism (T-RFLP) analysis. Diversity between the original parents and the pure inbred line may show significant differences in terms of richness, evenness and diversity indices. Heat maps reveal astonishing contributions of both or either parents (IR29 x Pokkali and AT401 x IR31868) in the shaping of the bacterial seed endophytes of the succeeding pure inbred line from the original hybrid host. Most of the T-RFs of the subsequent pure inbred line (FL478 and IC32) could be traced to any or both of the parents. Comparison of common and genotype-specific T-RFs of parents and their offspring reveals that majority of the T-RFs are shared suggesting higher transmission of bacterial communities common to both parents. The parents influence the bacterial community of their offspring. Unique T-RFs of the offspring also suggest external sources of colonization particularly as the seeds are cultivated in different ecogeographical locations. This study showed that host parental lines contributed greatly in the shaping of bacterial seed endophytes of their offspring. It also revealed transmission and potential conservation of core seed bacterial endophytes that generally become the dominant microbiota in the succeeding generations of plant hosts.
김기윤 ( Kiyoon Kim ),( Denver Walitang ),( Sandipan Samaddar ),( Shamim Ahmed ),사동민 ( Tongmin Sa ) 한국환경농학회 2017 한국환경농학회 학술대회집 Vol.2017 No.-
Soil salinity is a major abiotic factor related to microbial community structure and fungi have also been reported to be more sensitive to salinity stress than bacteria. Saemangeum reclaimed land is a part of Saemangeum Development Project. Most of the persistent problems of Saemangeum reclaimed land remain to be related to soil salinity. The aim of this study was investigate the effect of soil salinity levels on the microbial communities in Saemangeum reclaimed land using 454 pyrosequencing analysis. Soil samples were collected from 12 sites of Gyehwa area in Saemangeum reclaimed land. For pyrosequencing, 27F/518R (bacteria) and ITS3/ITS4 (fungi) primers were used containing the Roche 454 pyrosequencing adaptorkey- linker (underlined) and unique barcodes (X). Pyrosequencing was performed by Chun`s Lab using the standard shotgun sequencing reagents and a 454 GS FLX Titanium sequencing System (Roche, Inc.). Preprocessing and sequence analyses of fasta files containing the trimmed sequences were done using the Mothur application. In the soil samples, Proteobacteria and Ascomycota showed the highest relative abundance in all the soil sample sites. Proteobacteria, Bacteroidetes, Plantomycetes, Gemmatimonadetes and Parcubacteria were shown to have significantly higher abundance in high salinity level soils than low salinity level soils, while Acidobacteria and Nitrospirae have significantly higher relative abundance in low salinity level soils. The abundance of fungal, Basidiomycota, Zygomycota, Glomeromycota and Cerozoa had significantly higher relative abundance in low salinity level soils. Principal coordinate analysis (PCoA) and correlation analysis showed the salinity-related soil parameters affected bacterial and fungal community structure. Proteobacteria, Bacteroidetes and Plantomycetes exhibited significantly positive correlation with soil salinity, while Acidobacteria exhibited significantly negative correlation. In the case of fungal community, Basidiomycota and Zygomycota were seen to show significantly negative correlation with salinity related soil parameters. These results show that salinity and salinity-related soil parameters greatly influence the bacterial and fungal community structure in Saemangeum reclaimed land. This study also suggests provide understanding soil salinity that are important and potential key players on microbial community of Saemangeum reclaimed land.