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Dynamic analysis for gene expression profiles of endothelial colony forming cells under hypoxia
De-Cai Yu,Wen-Du Feng,Xian-Biao Shi,hi-Yong Wang,Wei Ge,Chun-Ping Jiang,Xi-Tai Sun,Yi-Tao Ding 한국유전학회 2013 Genes & Genomics Vol.35 No.4
Previous studies have shown that endothelial colony forming cells (ECFCs) play an important role in the neovascularization of tumors. Hypoxia is emphasized as an important promoter of angiogenesis. However, little is known about genome-wide transcriptional regulation of ECFCs under hypoxic conditions. In this study, gene expression profiles in ECFCswere evaluated under hypoxic conditions for 3, 6, 12, 24,and 48 h, using Affymetrix U133 plus 2.0 chip microarray. 1,103 hypoxia-regulated genes were filtered, with 379(0.693 %) genes up-regulated and 724 (1.32 %) genes downregulated. Most of the up-regulated genes were involved in apoptosis, cell proliferation, or metabolic processes, while most of the down-regulated genes were involved in cell adherence,cell cycle,DNAandmRNAmetabolic processes,multi-cellular organism development, protein metabolic processes, response to stress, signal transduction, or transport. This expression profile is ECFC-specific, because it is significantly different from those of endothelial cells and smooth muscle cells under hypoxic conditions. Moreover, hypoxia-regulated apoptosis in ECFCs is mainly related with the mitochondrial pathway (p53-BAX-Caspase-9) and the death receptor pathway (FASCaspase-8-Caspase-3). MAPK pathway is activated in ECFCs under hypoxic conditions. The differentially expressed genes of ECFCs were identified under hypoxic conditions, and related with cell apoptosis, cell cycle and MAPK pathways, shedding light on the mechanism of angiogenesis.
The GM Model of Application and Inspection in Harbin Local Tax Revenue Forecasts
De-fa Cai,Ru-yun Dai,JingCai 보안공학연구지원센터 2015 International Journal of u- and e- Service, Scienc Vol.8 No.5
GM (n, h) model is the model of grey system modeling, which uses n-order differential equations of h variables. N and h in different models have different meanings and uses[1].Use of model GM(1,1) and model GM(1,h) to tax revenues of the grey system model is established, and applied to the Harbin local tax revenue and the main tax prediction and verification, which provide intellectual support for the local government decision-making is very important [2].At first, this paper forecast the Harbin local tax amount, then respectively to forecast the main tax and inspection (value added tax, business tax, enterprise income tax, individual income tax),Through to the amount of tax and the main tax predicted results of the inspection, show that the prediction error is not big, in line with the actual.
( De Cai Jin ),( Ren Xing Liang ),( Qin Yun Dai ),( Rui Yong Zhang ),( Xue Liu Wu ),( Wei Liang Chao ) 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.10
Rhodococcus sp. JDC-11, capable of utilizing di-n-butyl phthalate (DBP) as the sole source of carbon and energy, was isolated from sewage sludge and confirmed mainly based on 16S rRNA gene sequence analysis. The optimum pH, temperature, and agitation rate for DBP degradation by Rhodococcus sp. JDC-11 were 8.0, 30oC, and 175 rpm, respectively. In addition, low concentrations of glucose were found to inhibit the degradation of DBP, whereas high concentrations of glucose increased its degradation. Meanwhile, a substrate utilization test showed that JDC- 11 was also able to utilize other phthalates. The major metabolites of DBP degradation were identified as monobutyl phthalate and phthalic acid by gas chromatographymass spectrometry, allowing speculation on the tentative metabolic pathway of DBP degradation by Rhodococcus sp. JDC-11. Using a set of new degenerate primers, a partial sequence of the 3,4-phthalate dioxygenase gene was obtained from JDC-11. Moreover, a sequence analysis revealed that the phthalate dioxygenase gene of JDC-11 was highly homologous to the large subunit of the phthalate dioxygenase from Rhodococcus coprophilus strain G9.
Big Enterprise Tax Risk Management: Warning, Simulation and Application
De-fa Cai,Nan-nan Ni,Jing Cai 보안공학연구지원센터 2015 International Journal of u- and e- Service, Scienc Vol.8 No.4
Currently, the construction of “promoting tax compliance as the goal, the risk management as the guidance, taking the information tube tax and the hierarchical classification as the basis, set up tax services, risk monitoring, risk response to the new tax management system”, this tax sources of specialized management model has become the direction of the reform of tax system, tax risk management as a new concept received extensive attention of tax authorities. Big enterprises are of the characteristics of small numbers, large scales, huge taxes and contributions, which is the core of tax collecting management. To strengthen the tax risk management ideas, and to direct taxpayers to perfect the inner tax control system are the important measures to implement individual services and pertinent management. This paper presents a flow chart of tax risk control and the four models: the identification model, the measurement model, the response model, the evaluation model, and simulation applications. Here, we take value-added tax as an example. In 2013, China's domestic value-added tax revenue is 2.8803 trillion yuan, accounting for 26% of the total income tax revenue, is one of China's current major taxes. Therefore, based on the data in the CTAIS to analyze the tax burden, and constructs the VAT tax burden warming system, is the effective measures to keep the rapid growth of VAT.
Jin, Cai De,Kim, Moo Hyun,Kim, Soo Jin,Lee, Kwang Min,Kim, Tae Hyung,Cho, Young-Rak,Serebruany, Victor L. S. Karger AG 2017 Cardiology Vol.137 No.2
<P><B><I>Background:</I></B> The optimal strategy to manage chronic total occlusion (CTO) remains unclear. The Japanese CTO multicenter registry (J-CTO) score is an established tool for predicting successful recanalization. However, it does not take into account nonangiographic predictors for final technique success. In the present study, we designed and tested a scoring model called the Busan single-center CTO registry (B-CTO) score combining clinical and angiographic characteristics to predict successful CTO recanalization in Korean patients. <B><I>Methods:</I></B> Prospectively enrolled CTO patients (<I>n</I> = 438) undergoing coronary intervention (1999-2015) were assessed. The B-CTO score comprises 6 independent predictors: age 60-74 years and lesion length ≥20 mm were assigned 1 point each, while age ≥75 years, female gender, lesion location in the right coronary artery, blunt stump, and bending >45° were assigned 2 points each. For each predictor, the points assigned were based on the associated odds ratio by multivariate analysis. The lesions were classified into 4 groups according to the summation of points scored to assess the probability of successful CTO recanalization: easy (score 0-1), intermediate (score 2-3), difficult (score 4-5), and very difficult (score ≥6). CTO opening was designated as the primary endpoint regardless of the interventional era or the skill of the operator. <B><I>Results:</I></B> The final success rate for B-CTO was 81.1%. The probability of successful recanalization for patient groups classified as easy (<I>n</I> = 64), intermediate (<I>n</I> = 148), difficult (<I>n</I> = 134), and very difficult (<I>n</I> = 92) was 95.3, 86.5, 79.1 and 65.2%, respectively (<I>p</I> for trend <0.001). When compared to the J-CTO, the B-CTO score demonstrated a significant improvement in discrimination as indicated by the area under the receiver-operator characteristic curve (AUC 0.083; 95% CI 0.025-0.141), with a positive integrated discrimination improvement of 0.042 and a net reclassification improvement of 56.0%. <B><I>Conclusions:</I></B> The B-CTO score has been designed and validated in Korean patients with native coronary CTO and is an improved tool for predicting successful recanalization. Wider application of the B-CTO score remains to be explored.</P>