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De Sun,Ping Yang,Lin Li,Hai-Hua Yang,Bing-Bing Li 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.10
A novel PDMS-PTFE/PVDF hollow fiber composite membrane using PTFE-PDMS as the top active layerand PVDF hollow fiber as the support layer was prepared for the pervaporation of chloroform from water. Sorptionand diffusion behaviors of chloroform and water in PDMS-PTFE membranes, which had different PTFE content, wereinvestigated. The results showed that with increasing PTFE content from 0 wt% to 40 wt%, chloroform/water idealseparation factor first increased and then decreased, and permeabilities of both chloroform and water increased. Forthe 30% PTFE-PDMS membrane, when feed temperature ranged from 40 oC to 60 oC, permeabilities of both chloroformand water increased, but ideal separation factor for chloroform/water first increased and then decreased. Effects of op-erating conditions, such as feed flow rate, active layer thickness, feed concentration and feed temperature, on PV per-formances of the 30% PDMS-PTFE/PVDF hollow fiber membrane for the pervaporation of chloroform-water mixtureswere studied. Examination showed that concentration polarization on the membrane surface occurred when feed flowrate was less than 3,000 mL/min. With the increase in active layer thickness from 13.8 to 78.0 µm, chloroform fluxand water flux dropped from 21 to 13 g·h−1·m−2and from 93 to 22 g·h−1·m−2, respectively, but the separation factorincreased from 1494 to 3949. With an increase of feed concentration from 55 to 850 ppm, chloroform flux increasedlinearly but water flux remained constant, and separation factor decreased. With an increase of the feed temperaturefrom 40 to 60 oC, both flux and separation factor increased, the variation of permeation flux followed the Arrheniusrelationship, and the activation energy values were 21.65 and 9.6 KJ/mol for water and chloroform, respectively.
De-Hai Li,Tian-Jiao Zhu,Hong-Bing Liu,Yu-Chun Fang,Qian-Qun Gu,Wei-Ming Zhu 대한약학회 2006 Archives of Pharmacal Research Vol.29 No.8
Four known butenolides were isolated from the ethyl acetate extracts of the culture broth of the marine-derived bacterium, Streptoverticillium luteoverticillatum, by bioassay-guided fractionation. The structures were identified on the basis of spectral data. The absolute configuration of compound (1) was determined by CD spectrum for the first time. Compounds 1-4 showed in vitro cytotoxicity against the murine lymphoma P388 and human leukemia K562 cell lines. This is the first report on the isolation of butenolides from the marine bacterium, Streptoverticillium luteoverticillatum, and their cytotoxic activities.
Baimantuoluosides D-G, Four New Withanolide Glucosides from the Flower of Datura metel L.
Bing-you Yang,Yong-gang Xia,Qiu-hong Wang,De-qiang Dou,Hai-xue Kuang 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.8
In our search for bioactive anti-psoriasis compounds from the flower of Datura metel L, we isolated four new withanolide glucosides, baimantuoluosides D, E, F and G (1-4). The structures of the new compounds are (5α,6α,7β,22R)-5,6,7,27-tetrahydroxy-1-oxowitha-2,24-dien-27-O-β-D-glucopyranoside (1), (5α,6β,7α,22R)-5,6,7,27-tetrahydroxy-1-oxowitha-2,24-dien-27-O-β-D-glucopyranoside (2), (5α,6β,7α,12β,22R)-5,6,7,12,27-pentahydroxy-1-oxowitha-2,24-dien-27-O-β-D-glucopyranoside (3), and (5α,6β,22R)-5,6,27-trihydroxy-1-oxowitha-2,24-dien-27-O-β-D-glucopyranoside (4) on the basis of chemical and physicochemical evidence.
MicroRNA Let-7c inhibits Bcl-xl expression and regulates ox-LDL-induced endothelial apoptosis
( Bing Qin ),( Bo Xiao ),( De Sheng Liang ),( Ye Li ),( Ting Jiang ),( Huan Yang ) 생화학분자생물학회(구 한국생화학분자생물학회) 2012 BMB Reports Vol.45 No.8
Endothelial cells (ECs) apoptosis induced by oxidized low-density lipoprotein (ox-LDL) is thought to play a critical role in atherosclerosis. MicroRNAs (miRNAs) are a class of noncoding RNAs that posttranscriptionally regulate the expression of genes involved in diverse cell functions, including differentiation, growth, proliferation, and apoptosis. MiRNA let-7 family is known to be involved in the regulation of cell apoptosis. However, the function of let-7 in ox-LDL induced ECs apoptosis and atherosclerosis is still unknown. Here, we show that let-7c expression was markedly up-regulated in ox-LDL induced apoptotic human umbilical cord vein endothelial cells (HUVECs). Let-7c over-expression enhanced apoptosis in ECs whereas inhibition of let-7c could partly alleviate apoptotic cell death mediated by ox-LDL. Searching for how let-7c affected apoptosis, we discovered that antiapoptotic protein Bcl-xl was a direct target of let-7c in ECs. Our data suggest that let-7c contributes to endothelial apoptosis through suppression of Bcl-xl. [BMB Reports 2012; 45(8): 464-469]
De-guo Yang,Yong-feng He,Xing-bing Wu,Yong-jiu Zhu 한국유전학회 2016 Genes & Genomics Vol.38 No.9
In order to elucidate the role of Cyp19a in sex differentiation of Schizothorax kozlovi, the full length cDNA of Cyp19a was cloned from the mature ovary of S. kozlovi by using rapid amplification of cDNA ends method, and then its relative mRNA expression levels among tissues and temperature groups were determined by using quantitative real-time PCR. The complete Cyp19a cDNA of 1795 bp of S. kozlovi was obtained, which encoded 517 amino acids and belonged to gonadal aromatase. Its deduced amino acid sequence had the above 70 % identity compared with gonadal aromatase genes of teleost fishes, but only 62–67 % when compared with brain aromatase genes of fishes. It was expressed only in heart and gonad, but no expression in other tissues, presenting relatively high tissue specificity. It also exhibited sex-specific expression pattern in gonads, but no sex differences in heart. Comparing with the Cyp19a expression levels at 12 days post hatching (dph), significant temperature effects were revealed in low temperature group (10 C) at 18 dph, and in high temperature group (26 C) at 40 dph. It suggested that gonadal aromatase Cyp19a gene may play important roles on the feminization or masculinization of S. kozlovi affected by temperature during the early developmental stage.
Li, De-Hai,Zhu, Tian-Jiao,Liu, Hong-Bing,Fang, Yu-Chun,Gu, Qian-Qun,Zhu, Wei-Ming The Pharmaceutical Society of Korea 2006 Archives of Pharmacal Research Vol.29 No.8
Four known butenolides were isolated from the ethyl acetate extracts of the culture broth of the marine-derived bacterium, Streptoverticillium luteoverticillatum, by bioassay-guided fractionation. The structures were identified on the basis of spectral data. The absolute configuration of compound (1) was determined by CD spectrum for the first time. Compounds 1-4 showed in vitro cytotoxicity against the murine lymphoma P388 and human leukemia K562 cell lines. This is the first report on the isolation of butenolides from the marine bacterium, Streptoverticillium luteoverticillatum, and their cytotoxic activities.
Zhang, Bing-Hui,Liu, Wei,Li, Liang,Lu, Jian-Guang,Sun, Ya-Nan,Jin, De-Jun,Xu, Xiu-Yu Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.6
Objective: The current study explored the expression of KAI1/CD82 and MRP1/CD9 and its significance in laryngeal squamous cell carcinoma (LSCC). Methods: The expression levels of KAI1/CD82 and MRP1/CD9 in 100 LSCC tissue specimens, as well as in 30 para-LSCC non-carcinomatous tissue specimens randomly taken from the patients, were assessed using the quantitative polymerase chain reaction (Q-PCR) and immunohistochemistry and correlations with pathological parameters of LSCC and their influence on survival function were analyzed. Results: KAI1/CD82 and MRP1/CD9 showed basically consistent changes in both mRNA and protein expression. Their expression in the 30 LSCC specimens was significantly lower compared with that in the corresponding non-carcinous tissues (P < 0.01 or 0.05), notably correlating with TNM stage, differentiation degree, clinical stage, and lymphatic metastasis (P < 0.01 or 0.05), but not gender, age, and LSCC growth sites (P > 0.05). The median survival of patients with positive KAI1/CD82 and MRP1/CD9 protein expression was longer than that of patients with negative protein expression (P < 0.01 or 0.05). KAI1/CD82 protein expression negatively correlated with MRP1/CD9 protein expression in LSCC (${\chi}^2$= 31.25, P < 0.01). Conclusion: KAI1/CD82 and MRP1/CD9 may jointly participate in the development of LSCC. They may serve as the markers for judging the infiltration, metastasis, and prognosis of LSCC.
Ping Wang,Bing Li,Yu-Ting Pan,Yun-Zhao Zhang,De-Wei Li,Lin Huang 한국식물병리학회 2020 Plant Pathology Journal Vol.36 No.5
The ascomycete fungus Colletotrichum gloeosporioides infects a wide range of plant hosts and causes enormous economic losses in the world. The transcription factors (TFs) play an important role in development and pathogenicity of many organisms. In this study, we found that the C2H2 TF CgCrzA is localized in both cytoplasm and nucleus under standard condition, and it translocated from cytoplasm to nucleus in a calcineurin- dependent manner. Moreover, the ΔCgCrzA was hypersensitive to cell wall perturbing agents and showed severe cell wall integrity defects. Deletion of the CgCRZA inhibited the development of invasive structures and lost pathogenicity to plant hosts. Our results suggested that calcineurin-responsive TF CgCrzA was not only involved in regulating cell wall integrity, but also in morphogenesis and virulence in C. gloeosporioides.
Fei Liu,Bing-Bing Li,De Sun,Fenggang Li,Xinyue Pei 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.5
Membrane hydrophilicity is a crucial factor in evaluating ultrafiltration processes. In this paper, chitosan(CS) was selected for the hydrophilic modification of the polylactic acid (PLA) membrane, and PLACS membranes wereprepared for the densification of the yeast solution. By non-solvent phase inversion method (NIPS), the PLACS microfiltrationmembranes were prepared by using chitosan (CS) acetic acid solution as the coagulation bath and glutaraldehydeas the crosslinking agent. PLACS membranes were characterized by water contact angle, porosity, pore sizedistribution, mechanical properties, ATR-FTIR, SEM, TGA and the ultrafiltration experiment. The viscosity of coagulationbath solution can severely influence the exchange rate of the solvent and the non-solvent as well during phaseinversion; therefore, it can regulate the precipitation kinetics and membrane morphology. The results showed that chitosan(CS) was presented as granular on the pore surfaces of the PLACS membranes. When chitosan (CS) contentincreased, gel rate became smaller and membrane forming process was prolonged; the porosity and pore size of thePLACS were increased compared to the polylactic acid (PLA) membrane, pure water flux increased from 90.31 L·m2·h1to 120.14 L·m2·h1, and yeast rejection rate increased from 75% to more than 90%.