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Immunocytocalization of Cell Wall Peroxidase and Other Wall Antigens from Maize Seedlings
Kim, Sung-Ha,Dauwalder, Marianne,Roux, Stanley J. Jr. 한국식물학회 1996 Journal of Plant Biology Vol.39 No.2
Immunocytochemistry utilizes the specificity of the antigen-antibody reaction to localize specific antigens in cells or cellular organelles. Here we report the use of monoclonal antibodies, in conjunction with gold-labeled second antibodies to study the ultrastructural localization and tissue distribution of the Mr 98,000 anionic peroxidase and other wall antigens. The antibody specific for this wall peroxidase, mWP3, labeled mainly the cell wall area. At the tissue level, the Mr 98,000 peroxidase is located predominantly in the leaf mesophyII, internal coleoptile and sieve elements, but not in the root, s assayed with these procedures. The coleoptile walls were less heavily stained than the walls of leaf mesophyII cells. At the subcellular level, it is localized mainly in intercellular regions of the cell walls. A similar staining pattern was revealed by mWP19, one of anti-β glucosidase antibody, though it looked less heavily stained than one with mWP3. In order to serve as a control wall staining using IgM monoclonal antibodies, mWP18 was used. Most of the label is localized over wall regions of cells of the young leaf mesophyII and coleoptile.
Sandrine Heraud,Anne-Marie Freydiere,Anne Doleans-Jordheim,Michèle Bes,Anne Tristan,François Vandenesch,Frederic Laurent,,Olivier Dauwalder 대한진단검사의학회 2015 Annals of Laboratory Medicine Vol.35 No.4
Staphylococcus aureus bacteremia is associated with high mortality and morbidity, requiring prompt and appropriate antimicrobial treatment. Therefore, it is important to detect methicillin-resistant S. aureus (MRSA) rapidly from blood cultures. Two immunochromatographic tests, BinaxNow S. aureus and BinaxNow PBP2a, were directly applied to 79 Bact/Alert bottles that were positive for Gram positive cocci in cluster aggregations. Sensitivity and specificity for the identification of S. aureus and determination of methicillin resistance were 94% and 87%, and 100% and 100%, respectively, with less than 30 min of performance time. These tests are efficient and rapid; these tests are valuable alternatives to more sophisticated and expensive methods used in the diagnosis of MRSA bacteremia.