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( Dang Hai Dang Nguyen ),( Sung-hoon Park ),( Phuong Lan Tran ),( Jung-wan Kim ),( Quang Tri Le ),( Winfried Boos ),( Jong-tae Park ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.3
We first confirmed the involvement of MalQ (4-α-glucanotransferase) in Escherichia coli glycogen breakdown by both in vitro and in vivo assays. In vivo tests of the knock-out mutant, ΔmalQ, showed that glycogen slowly decreased after the stationary phase compared to the wild-type strain, indicating the involvement of MalQ in glycogen degradation. In vitro assays incubated glycogen-mimic substrate, branched cyclodextrin (maltotetraosyl-β-CD: G4-β-CD) and glycogen phosphorylase (GlgP)-limit dextrin with a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ. In the absence of GlgP, the reaction of MalP, GlgX and MalQ on substrates produced glucose-1-P (glc-1-P) 3-fold faster than without MalQ. The results revealed that MalQ led to disproportionate G4 released from GlgP-limit dextrin to another acceptor, G4, which is phosphorylated by MalP. In contrast, in the absence of MalP, the reaction of GlgX, GlgP and MalQ resulted in a 1.6-fold increased production of glc-1-P than without MalQ. The result indicated that the G4-branch chains of GlgP-limit dextrin are released by GlgX hydrolysis, and then MalQ transfers the resultant G4 either to another branch chain or another G4 that can immediately be phosphorylated into glc-1-P by GlgP. Thus, we propose a model of two possible MalQ-involved pathways in glycogen degradation. The operon structure of MalP-defecting enterobacteria strongly supports the involvement of MalQ and GlgP as alternative pathways in glycogen degradation.
The Relationship between a Mega Sport Event and the Host Destination
Dang Thi Phuong Anh 한국관광학회 2014 International Journal of Tourism Sciences Vol.14 No.3
Tourism and sport are key elements of the culture in many countries and can influence the behavior of society. Especially since the 1960’s, mega sport events have become a huge international draw card garnering a large amount of media attention, and attracting investment and also political interest. Tourism remains the world’s largest industry and continues to develop and grow. The aim of this study is to assess the relationship between a mega sport event and the host destination. To address the aim, the research was conducted in two stages: the first stage was a literature review which presents a deeper understanding of the two-way relationship: (i) Mega sport events create opportunities for the host destination and (ii) The host destination attracts tourists to attend the mega sport event. The second stage explores two methods destinations can use to host a mega sport event successful.
Presence of β-Amylase in Ramie Leaf and its Anti-staling Effect on Rice Cake
Dang Hai Dang Nguyen,Phuong Lan Tran,하현숙,이진실,홍완수,Quang Tri Le,오병철,박성훈 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.1
Presence of β-amylase in ramie leaf and its anti-staling effect on starch-based foods were assessed. The ammonium sulfate fractionate (80% saturation) of the ramie leaf extracts showed a β-amylase activity, giving maltose (Glc2) as a major product, exclusively, when incubating with maltopentaose (Glc5) or soluble starch at 45oC, pH 6.0. The starch-based food product (rice cake) prepared with freeze-dried ramie leaf enzyme revealed that the linear maltooligosaccharides ranging from Glc2 to Glc6 significantly increased and the shorter branch chains (DP<15) of amylopectin increased whereas the longer branch chains (DP>16) decreased in the product. These results demonstrated that maltosyl residue was released from the non-reducing end of the longer branch chains of amylopectin by β-amylase. The ramie leaf-treatment sample significantly reduced the retrogradation rate during 48 h storage at 4℃. As an alternative plant-origin enzyme, the ramie leaf β-amylase has potential for a novel anti-staling additive.
Nguyen, Dang Hai Dang,Park, Jong-Tae,Shim, Jae-Hoon,Tran, Phuong Lan,Oktavina, Ershita Fitria,Nguyen, Thi Lan Huong,Lee, Sung-Jae,Park, Cheon-Seok,Li, Dan,Park, Sung-Hoon,Stapleton, David,Lee, Jin-Sil American Society for Microbiology 2014 Journal of Bacteriology Vol.196 No.11
<P>We studied the activity of a debranching enzyme (TreX) from <I>Sulfolobus solfataricus</I> on glycogen-mimic substrates, branched maltotetraosyl-β-cyclodextrin (Glc<SUB>4</SUB>-β-CD), and natural glycogen to better understand substrate transglycosylation and the effect thereof on glycogen debranching in microorganisms. The validation test of Glc<SUB>4</SUB>-β-CD as a glycogen mimic substrate showed that it followed the breakdown process of the well-known yeast and rat liver extract. TreX catalyzed both hydrolysis of α-1,6-glycosidic linkages and transglycosylation at relatively high (>0.5 mM) substrate concentrations. TreX transferred maltotetraosyl moieties from the donor substrate to acceptor molecules, resulting in the formation of two positional isomers of dimaltotetraosyl-α-1,6-β-cyclodextrin [(Glc<SUB>4</SUB>)<SUB>2</SUB>-β-CD]; these were 6<SUP>1</SUP>,6<SUP>3</SUP>- and 6<SUP>1</SUP>,6<SUP>4</SUP>-dimaltotetraosyl-α-1,6-β-CD. Use of a modified Michaelis-Menten equation to study substrate transglycosylation revealed that the <I>k</I><SUB>cat</SUB> and <I>K<SUB>m</SUB></I> values for transglycosylation were 1.78 × 10<SUP>3</SUP> s<SUP>−1</SUP> and 3.30 mM, respectively, whereas the values for hydrolysis were 2.57 × 10<SUP>3</SUP> s<SUP>−1</SUP> and 0.206 mM, respectively. Also, enzyme catalytic efficiency (the <I>k</I><SUB>cat</SUB>/<I>K<SUB>m</SUB></I> ratio) increased as the degree of polymerization of branch chains rose. In the model reaction system of <I>Escherichia coli</I>, glucose-1-phosphate production from glycogen by the glycogen phosphorylase was elevated ∼1.45-fold in the presence of TreX compared to that produced in the absence of TreX. The results suggest that outward shifting of glycogen branch chains via transglycosylation increases the number of exposed chains susceptible to phosphorylase action. We developed a model of the glycogen breakdown process featuring both hydrolysis and transglycosylation catalyzed by the debranching enzyme.</P>
Development of plastic fender for weight-reduced automobiles
Xuan-Phuong Dang,Hong-Seok Park 한국자동차공학회 2010 한국자동차공학회 학술대회 및 전시회 Vol.2010 No.11
Lightweight design with thermoplastics is a potential approach in order to reduce the weight and fuel consumption. This paper reviews the role of plastics and lightweight design as well as the state of the art and develops the design process for changing steel fender to plastic ones. The plastic car fenders made by injection molding method that offer some advantages such as light weight, low cost, recycle-ability, manufacturability and design flexibility can replace the traditional on-line painted steel car fender. In this study, design concepts, material section, and optimization strategy in terms of design for strength, and design for manufacturability were proposed. CAE tools were used to calculate the strength and predict the thermal expansion of the fender. The simulation results show that large thermal expansion is the disadvantage of the thermal plastic fenders, so special fastening devices were adopted. The weight of plastic car fender can be reduced two times compared to the steel fender. A real mold for Sonata 2008 fender was made and the testing process was carried out to check the manufacturability and optimization results.
Design of U-shape Milled Groove Conformal Cooling Channels for Plastic Injection Mold
Xuan-Phuong Dang,박홍석 한국정밀공학회 2011 International Journal of Precision Engineering and Vol. No.
Besides the solid free-form fabrication technology, milling operation is an alternative applicable method to make complex cooling channels conform to the surface of the mold cavity. This paper presents the U-shape milled groove conformal cooling channels and proposes the design optimization process in order to obtain an optimal cooling channels’configuration and target mold temperature. The relation between the cycle averaged thermal behavior of the mold cavity and the two-dimensional configuration of cooling channels was first investigated thoroughly by an analytical method. Design of experiment and 2D simulation were done to obtain the mold wall temperature and to check the feasibility of the analytical method. The optimization process of the free-form conformal cooling channels is based on the combination of both analytical method and 3D CAE simulation. The analytical step relies on explicit mathematic formulas, so it can approach the neighboring optimal solution quickly. Subsequently, the three-dimensional heat transfer simulation is applied to fine-tune the optimization results. A case study for a plastic car fender was investigated to verify the feasibility of the proposed method. The results show that conformal cooling channel gives a uniform cooling, reducing the cooling time and increasing the molded part’s quality with less effort of plastic designers and high computational efficiency.
Nguyen Phuong Thuy(Nguyen Phuong Thuy ),Nguyen Ngoc Trai(Nguyen Ngoc Trai ),Bui Dang Khoa(Bui Dang Khoa ),Nguyen Hoang Xuan Thao(Nguyen Hoang Xuan Thao ),Vuong Tuan Phong(Vuong Tuan Phong ),Quach Van 한국육종학회 2023 Plant Breeding and Biotechnology Vol.11 No.2
Genetic variability and correlation analysis are fundamental references for the innovative development of breeding programs to improve varieties and desirable traits. In the present study, the correlation and path analysis was conducted to understand the association among yield, micronutrients (iron and zinc), and protein content under aerobic conditions in local rice landraces from various agro ecological regions of Karnataka, India. The grain yield per plant showed a significant positive correlation with plant height, the tiller number, spikelet fertility, flag leaf length, and test weight. The zinc content was negatively correlated with grain yield per plant. The phenotypic path-coefficient analysis revealed that the total number of tillers, grain length, test weight, and harvest index exhibited a positive direct effect on grain yield per plant, while Grain protein content showed a low direct effect on this trait. This study also indicated that harvest index, flag leaf length, spikelet fertility, and test weight could be considered to make for a higher yield.