Inactivation of Foodborne Pathogens by Lactic Acid Bacteria

Frank Daliri,Agnes Achiaa Aboagye,Eric Banan-Mwine Daliri 한국식품위생안전성학회 2020 한국식품위생안전성학회지 Vol.35 No.5

The problems caused by foodborne pathogens are not only a concern to the food industry but also with regard to global public health. Over the years, fermentation technology has proved to be one of the cheapest and safest methods for inactivating and controlling pathogenic microorganisms in food. Scientific evidence shows that lactic acid bacteria fermentation exerts significant antimicrobial effect against pathogenic bacteria and viruses. Lactic acid bacteria metabolites such as organic acids, bacteriocins and hydrogen peroxides have adverse effects on foodborne pathogens which lead to their inhibition. These compounds do not only cause physical injuries, but also have significant effects on the pathogens' gene expression. Furthermore, the presence of lactic acid bacteria in food provides nutritional competition among foodborne pathogens, and all these factors together suppress their growth. This study reviews our current knowledge of the antimicrobial abilities of lactic acid bacteria, their molecular mechanisms, and their application for inactivating foodborne pathogens.

Immunosensors for Food Safety: Current Trends and Future Perspectives

Frank Daliri,Agnes Achiaa Aboagye,Vincent Kyei-Baffour,ELAHI MD FAZLE,CHELLIAH RAMACHANDRAN,Eric Banan-Mwine Daliri 한국식품위생안전성학회 2019 한국식품위생안전성학회지 Vol.34 No.6

To monitor the levels of antimicrobials, allergens, pathogens and other contaminants in foods meant for human consumption, it is imperative to have quick, accurate and low-cost tests. Advanced techniques (e.g. label-free biosensor assays) have been developed over the past 10?15 years to solve some of these problems. As biosensors, immunosensors can provide real-time measurements, a high degree of automation, and improved throughput and sensitivity. By comparison with conventional methods, immunosensors are less expensive, less sophisticated physicochemical instruments that require less time for analysis while also being more user-friendly. In this review, we discuss our current knowledge about immunosensors, their strengths and weaknesses, as well as the future of these biosensors in food safety.

Current trends and perspectives of bioactive peptides

Daliri, Eric Banan-Mwine,Lee, Byong H.,Oh, Deog H. Informa UK (TaylorFrancis) 2018 Critical reviews in food science and nutrition Vol.58 No.13

Novel angiotensin I-converting enzyme inhibitory peptides from soybean protein isolates fermented by <i>Pediococcus pentosaceus</i> SDL1409

Daliri, Eric Banan-Mwine,Lee, Byong H.,Park, Mi Houn,Kim, Jong-Hak,Oh, Deog-Hwan Elsevier 2018 LWT- Food science and technology Vol.93 No.-

<P>In this study, Pediococcus pentosaceus SDL1409 was used to ferment soybean protein isolates for 48h at 37 degrees C. Low molecular weight peptides (<= 7 KDa) from the fermented sample showed strong angiotensin I-converting enzyme inhibitory ability of 65.1 +/- 0.78% (IC50 = 0.123 +/- 0.02 mg protein/ml). The low molecular weight peptides were identified using liquid chromatography-electrospray ionization quantitative time-of-flight mass spectrometry analysis. On the basis of peptide abundance and common structural features, four peptides were selected and chemically synthesized. The peptides namely EDEVSFSP, SRPFNL, RSPFNL and ENPFNL showed strong IC50 values of 0.571 +/- 0.12, 0.131 +/- 0.02, 0.811 +/- 0.05 and 0.287 +/- 0.07mg/mL respectively. EDEVSFSP was fairly resistant to gastrointestinal enzyme digestion. The presence of P/FNL, the N-terminal sequence as well as the position of amino acids in the peptides were essential for ACE inhibition. Our data demonstrate that Pediococcus pentosaceus SDL1409 could be useful for developing antihypertensive functional foods from soybean proteins.</P>

The human microbiome and metabolomics: Current concepts and applications

Daliri, Eric Banan-Mwine,Wei, Shuai,Oh, Deog H.,Lee, Byong H. Informa UK (TaylorFrancis) 2017 Critical reviews in food science and nutrition Vol.57 No.16

<P>The mammalian gastrointestinal tract has co-developed with a large number of microbes in a symbiotic relationship over millions of years. Recent studies indicate that indigenous bacteria are intimate with the intestine and play essential roles in health and disease. In the quest to maintain a stable niche, these prokaryotes influence multiple host metabolic pathways, resulting from an interactive host-microbiota metabolic signaling and impacting strongly on the metabolic phenotypes of the host. Since dysbiosis of the gut bacteria result in alteration in the levels of certain microbial and host co-metabolites, identifying these markers could enhance early detection of diseases. Also, identification of these metabolic fingerprints could give us clues as to how to manipulate the microbiome to promote health or treat diseases. This review provides an overview of our current knowledge of the microbiome and metablomics, applications and the future perspectives.</P>

Development of a Soy Protein Hydrolysate with an Antihypertensive Effect

Daliri, Eric Banan-Mwine,Ofosu, Fred Kwame,Chelliah, Ramachandran,Park, Mi Houn,Kim, Jong-Hak,Oh, Deog-Hwan MDPI AG 2019 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.20 No.6

<P>In this study, we combined enzymatic hydrolysis and lactic acid fermentation to generate an antihypertensive product. Soybean protein isolates were first hydrolyzed by Prozyme and subsequently fermented with <I>Lactobacillus rhamnosus</I> EBD1. After fermentation, the in vitro angiotensin-converting enzyme (ACE) inhibitory activity of the product (P-SPI) increased from 60.8 ± 2.0% to 88.24 ± 3.2%, while captopril (a positive control) had an inhibitory activity of 94.20 ± 5.4%. Mass spectrometry revealed the presence of three potent and abundant ACE inhibitory peptides, PPNNNPASPSFSSSS, GPKALPII, and IIRCTGC in P-SPI. Hydrolyzing P-SPI with gastrointestinal proteases did not significantly affect its ACE inhibitory ability. Also, oral administration of P-SPI (200 mg/kg body weight) to spontaneous hypertensive rats (SHRs) for 6 weeks significantly lowered systolic blood pressure (−19 ± 4 mm Hg, <I>p</I> < 0.05) and controlled body weight gain relative to control SHRs that were fed with physiological saline. Overall, P-SPI could be used as an antihypertensive functional food.</P>

Current Perspectives on Antihypertensive Probiotics

Daliri, E. B.,Lee, B. H.,Oh, D. H. Springer Science + Business Media 2017 Probiotics and antimicrobial proteins Vol.9 No.2

<P>Hypertension is a major risk factor for cardiovascular diseases. Optimizing blood pressure results in an overall health outcome. Over the years, the gut microbiota has been found to play a significant role in host metabolic processes, immunity, and physiology. Dietary strategies have therefore become a target for restoring disturbed gut microbiota to treat metabolic diseases. Probiotics and their fermented products have been shown in many studies to lower blood pressure by suppressing nitrogen oxide production in microphages, reducing reactive oxygen species, and enhancing dietary calcium absorption. Other studies have shown that hypertension could be caused by many factors including hypercholesterolemia, chronic inflammation, and inconsistent modulation of the renin-angiotensin system. This review discusses the antihypertensive roles of probiotics and their fermented products via the reduction of serum cholesterol levels, anti-inflammation, and inhibition of angiotensin-converting enzyme. The ability of recombinant probiotics to reduce high blood pressure has also been discussed.</P>

Human microbiome restoration and safety

Daliri, Eric Banan-Mwine,Tango, Charles Nkufi,Lee, Byong H.,Oh, Deog-Hwan Elsevier 2018 International journal of medical microbiology Vol.308 No.5

<P><B>Abstract</B></P> <P>The human gut microbiome consists of many bacteria which are in symbiotic relationship with human beings. The gut microbial metabolism, as well as the microbial-host co-metabolism, has been found to greatly influence health and disease. Factors such as diet, antibiotic use and lifestyle have been associated with alterations in the gut microbial community and may result in several pathological conditions. For this reason, several strategies including fecal microbiota transplant and probiotic administration have been applied and proven to be feasible and effective in restoring the gut microbiota in humans. Yet, safety concerns such as potential health risks that may arise from such interventions and how these strategies are regulated need to be addressed. Also, it will be important to know if these microbiome restoration strategies can have a profound impact on health. This review provides an overview of our current knowledge of the microbiome restoration strategies and safety issues on how these strategies are regulated.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Magnetic Resonance Imaging and Sonographic Features before and after Surgery in Carpal Tunnel Syndrome: Association with Clinical Findings

Mahla Daliri,Motahareh Ebrahimnejad,Samane Najafi,Behzad Aminzadeh,Maryam Emadzadeh,Department of Neurosurgery, Shahid Beheshti University of Medical Scie,Ali Moradi 대한정형외과학회 2022 Clinics in Orthopedic Surgery Vol.14 No.4

Background: The interest in ultrasonography (US) and magnetic resonance imaging (MRI) assessment of the patients with carpal tunnel syndrome (CTS) is growing. This paper aimed to find the correlation of postoperative changes in these modalities’ parameters with clinical outcomes. Methods: Boston CTS questionnaire-symptom severity (BQ-SS), Boston CTS questionnaire-functional status (BQ-FS), and visual analog scale (VAS) questionnaires (for pain, paresthesia, and grip weakness assessment) were used to evaluate clinical outcomes. Various imaging parameters of the median nerve and carpal tunnel were evaluated using US and MRI at two levels of the hook of the hamate (distal) and the pisiform (proximal) once preoperatively and then 3 months postoperatively. Corresponding US and MRI parameter measures were compared, and correlational analysis was performed between alteration of imaging findings and changes in clinical parameters postoperatively. Results: Patients’ functional status (BQ-FS score) was positively correlated with the nerve width both in US and MRI at the proximal level (r = 0.457 and r = 0.453, respectively) and also with the MRI nerve circumference at the distal level (r = –0.482). Correlation between paresthesia and the nerve width was notable in MRI at the distal hook of the hamate level (r = –0.403). Grip weakness VAS score was correlated with the nerve width-to-height ratio (WHR) in US at the distal level (r = 0.432). Conclusions: Changes in US and MRI parameters of the median nerve width, circumference, and WHR were associated with clinical changes in patients with CTS after surgery.

Towards optimal slip force and stiffness distribution in designing friction dampers

Pourya Sam-Daliri,Seyed Mehdi Zahrai,Hamid Dahaghin 국제구조공학회 2021 Structural Engineering and Mechanics, An Int'l Jou Vol.79 No.3

The considerable capacity of friction dampers in energy dissipation makes them a good choice for vibration control of structures. The slip force of friction dampers and also the stiffness of the corresponding bracing system are the major parameters that must be chosen carefully in the design procedure of these dampers. This paper presents an innovative approach to determine these parameters using the data extracted from a series of analyses conducted on three different structures, subjected to five different earthquake records. For this purpose, 900 time-history analyses are conducted. The responses extracted from these analyses are used to compare the effect of different slip forces and to choose the optimum case. Also, a stiffness calibration method is proposed to determine the bracing system stiffness. Finally, two multi-functional optimization methods are introduced to find a single value for optimal slip force. It is shown that between 56 to 74% of the input energy can be dissipated by friction dampers, using this design approach. Additionally, up to 20, 45, 64, and 62% reductions in maximum displacement, velocity, acceleration, and base shear are achieved respectively for the structures studied in this research.