인간포배기 배아의 효과적인 유리화 동결법의 개발을 위한 연구

이상민,이주희,이상원,이승민,윤산현,임진호,박흠대,이성구,Lee, Sang-Min,Lee, Ju-Hee,Lee, Sang-Won,Lee, Seoung-Min,Yoon, San-Hyun,Lim, Jin-Ho,Park, Huem-Dai,Lee, Seong-Goo 대한생식의학회 2003 Clinical and Experimental Reproductive Medicine Vol.30 No.3

Objective: The purpose of this study was to evaluate the survival rate of vitrified blastocyst according to the freezing vessels, equilibration time in cryoprotectant and artificial dehydration method. Methods: Human blastocysts were vitrified after loading onto the plastic straw, open-pulled straw (OPS), electron microscopy grid (EM grid) for 1.5 min or 3 min. They also were directly plunged into LN2 within 30sec. For artificial shrinkage of blastocysts, 36 gauge fine needle was pushed at the cellular junction of the trophectoderm into the blstocoele cavity until it shrank without damage of inner cell mass. Results: The survival rate of vitrified blastocysts on plastic straw, OPS, EM grid as freezing vessels were 26.7, 13.0 and 60.5%, respectively. The survival rate of EM grid was significantly higher than that of plastic straw and OPS (p<0.05). For 1.5 min equilibrium, the survival rates of early blastocyst (EB), middle blastocyst (MB) and late blastocyst (LB) were 64.4, 81.0, and 20.0% respectively. For 3 min equilibrium, the survival rates of EB, MB, and LB were 69.9, 50.0 and 57.5% respectively. The survival rates of EB and MB were significantly higher than that of LB in 1.5 min equilibrium group (p<0.05), however, the significance was not observed in 3 min equilibrium groups. In cytoplasmic shrinkage before vitrification, the survival rates of EB, MB and LB were 92.9, 100 and 75.9% respectively. The survival rate of MB was significantly higher than that of LB (p<0.05). The survival rates of vitrified blastocysts by artificial dehydration and slow-frozen blastocysts were not significantly different as 88.9 and 66.7%, respectively. Conclusion: This study showed that the vitrification of human blastocysts using EM grid and artificial dehydration is an effective method. Therefore, these methods would be an useful techniques for blastocyst cryopreservation.

花崗岩土 開田地의 浸蝕-堆砂實態와 土砂收支에 對한 考察

閔大泓 公州大學校 資源科學硏究所 1994 資源科學硏究論文集 Vol.2 No.-

In this study, gully erosion and sedimentation on reclaimed land of decomposed granite soil were surveyed. The results were as follows. It was observed that sedimentation form of washed soil is different each other according to the layout and gradient of drainage channel or the structure and capaciety of sedimentation facilities. The efficient method to control soil which is washed out from the field is to construct the catch channel and use it as a sedimentation space. Constructing the horizontal channel crossing the field, the amount of gully erosion was decreased and washed soil was also decreased. It is effective method that the soil sedimentation facilities is constructed as a shape of rectangle, and sedimentation basin must be constructed in order that soil gravel is sedimented very well and washed water do not flow to the direction of spillway. The data of soil erosion and sedimentation in short time is necessary to solve the problem of reclaimed land in the granite soil zone.

AFB₁ 대사에서 phloretion의 이중 활성 효과

임대원,이광,고상상,진효연,은상용,최병민,김복량 圓光大學校 醫科學硏究所 2008 圓光醫科學 Vol.23 No.1

Aflatoxm B₁(AFB₁) is a potent hepatocarcinogen in experimental animals and a hazard to human health in several parts of the world. AFB₁ is activated to its ultimate carcinogenic intermediate, AFB₁-8,9-epoxide, by cytochrome P450(CYP) 1A2 and CYP3A4 in human liver and the intermediate is decomposed by several glutathione S-transferase(GST) including GSTA2, GSTM1 and GSTP1. In this study, we investigated the effects of phloretin on the enzyme systems which are involved in the activation and detoxification of AFB₁. The metabolic intermediate of AFB₁ was measured with HPLC. We found that phloretin could strongly inhibit the activities of CYP 3A4 and CYP1A2 in a dose dependent manner. Phloretin induced the antioxidant-response element(ARE)-mediated gene expression, including GSTs. The expressions of GSTA2, T1, M1, and GSTP1 were induced by 10μM phloretin. The decomposition of AFB₁-8,9-epoxide was measured with GSH conjugating activity of the epoxide. The rate was increased to 1.5 fold when HepG2 cells were treated by 10μM phloretin for 12h. In the mean while, the total GST activitives toward CDNB in HepG2 cells were not changed by the treatment with phloretin. The results demonstrate that phloretin has strong chemopreventive effects against AFB₁ toxicity through the inhibition of AFB₁ activation and induction of GSTs.

Hofmann 전이 반응을 이용한 N-(L-Aspartyl)-1,1-Diaminoethane의 합성

정대일,이용균,정일수,김윤영,김선영,박민수 東亞大學校 1998 東亞論叢 Vol.35 No.-

Some retroisomeric peptide showed an agonistic or antagonistic effect of the parent peptides. So the studies on their synthesis and their structure-activity relationship are currently being investigated. In general, the synthesis of the retroisomeric peptide required N,N'-diacylated gem-diamino compound as an important synthetic intermediate, in which those diacylated group could be removed selectively in different condition. In connection with the development of facile synthetic method for retroisomeric peptide, we tried the synthesis of N-benzoyl-1. l-diaminoethane, intermediate of 1, 1-diaminoethane based Sweetener, from N-benzoyl alanine aimde 28 using improved Hofmann rearrangement as a key step. In this procedure, the N-benzoyl-N'-Cbz-1,1-diaminoethane 29 could be prepared in high yield without any bypreducts. And also this compound was converted to N-benzoyl-1, 1-diaminoethane 30 by hydrogenolysis successfully. From the above result, this method thought to be very convenient synthetic method for these N-acyl-l, l-diamino typed compounds, a intermediate of retroisomeric peptides. And also β-benzyl aspartate, another intermediate of these sweetener, could be prepared from aspartic acid via dibenzylation and selective hydrolysis.

朝鮮時代 鄕校建築의 樣式에 關한 硏究 : 忠南地方 鄕校의 大成殿을 中心으로

李達勳,金賢錫,姜圭敏 대전대학교 산업기술연구소 1999 산업기술연구소 論文集 Vol.10 No.1

This paper, studying about Taesongjeon (the great shrine) which is in 11 Hyang-kyo (local school annexed to the confucian shrine) Architecture in Chung Nam local province, understands the meaning of 'Taesongjeon' from inspecting the specific regional feature of its construction type by analyzing various peculiarities and proposes the preservation of Hyang-gyo Architecture. The results of this paper are as following : 1) Generally, the placement types of Hyang-gyo Architecture in Chung Nam local province is 'School in the front and shrine in the back' 2) The shape of Taesongjeon plane has a open plane figure to worship and can be divided into 3 kans in front and 3 kans on sides. 3) The type of Gong-po of Taesongjeon is Mu-Chul-Mok Yi-Ikkong system and the form of the frame has been built with a higher pillar type.

랫드에서 신우황청심원의 급성 및 아급성독성시험

오승민,남혜윤,김준수,연제덕,신대희,이진영,박대규,조명행,정규혁 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

The acute and subacute toxicity of New Woohwangchungsimwon(NWCH) which was used l-muscone as substitutive material of musk were investigated in S.D. rats. In intraperitoneal acute toxicity test, rats(Sprague-Dawley, SPF) were injected intraperitoneally with dosages of 0, 540, 750, 1,070, 1,500 and 3,000 ㎎/㎏. Body weights were significantly decreased at 540 ㎎/㎏ dose group in both sexes and abnormal autopsy findings were founded in both sexes at all treated groups. Intraperitoneal LD_50 of NWCH was 812.3 ㎎/㎏ in male and 872.3 ㎎/㎏ in female rats. In the subacute toxicity study, NWCH was administrated orally to both sexes of rats for 4 weeks as several doses(0, 320, 800 and 2,000 ㎎/㎏). There were neither dead animals nor significant changes of body weights during the experimental period. In addition, no differences were found between control and treated groups in clinical signs, urinalysis, hematology, serum biochemical analysis, and other findings. Above data strongly suggest that no observed adverse effect level of NWCH might be over 2,000 ㎎/㎏/day in this study.

CSVT법에 의해 증착시킨 CdS 박막의 전기적 특성

김화민,최제헌,이병로,김태만,윤대현 대구효성가톨릭대학교 자연과학연구소 1991 基礎科學硏究論集 Vol.1991 No.1

CSVT법에 의해 증착시킨 CdS 박막의 전기적 특성 및 열전기 특성을 조사하기 위하여 시료의 X-선 회절, 직류 전기전도도, Hall 이동도 그리고 TSC를 측정하였다. 그 결과 CSVT 법에 의해 제작된 CdS 박막이 보통중착법에 의한 것에 비하여 결정성장이 향상되고 Hall 이동도가 크며 저항이 작다는 것을 알았다. 또한 TSC 측정결과, 광조사 조건에 따라 TSC 피크가 다르게 나타나며 이러한 피크 특성은 photochemical reaction model로서 설명될 수 있고, 그리고 제작조건이 다른 두 CdS 박막에서 나타나는 TSC 피크의 비교로부터 CSVT법에 의해 제작된 시료가 보통증착법에 의한 것보다 상온 이상에서의 photochemical reaction 효과 적음을 알 수 있다. To investigate electrical property and thermally stimulated current (TSC) for the CdS films which were grown by close-spaced vapor transport (CSVT), the measurements of X-ray diffraction pattern (XRD), d.c. conductivity, Hall mobility and TSC were carried out. Form the result of the measurements, CSVT-CdS films are found to be better oriented and to be less resistive with better mobility as compared with the films evaporated by the conventional method. TSC spectra under illumination conditions show different TSC peaks. These can be explained by photochemical reaction model. The result suggests that the effect of photochemical reaction above room temperature is to be less than conventional evaporated films in the case of CSVT-CdS films.

S-상 심실중격의 임상적 고찰

이동민,장경식,양종태,장대용,김종오,서영욱,고영엽,홍순표 조선대학교 부설 의학연구소 2002 The Medical Journal of Chosun University Vol.27 No.1

Background: The sigmoid septum described an anatomical variation in the contour of the left ventricular outlet. It is related to degenerative change with aging. We observed clinical significance of sigmoid septum in Korean. Methods: Echocardiographic examination was performed in 434 patients. Sigmoid septum is defined as discrete bulging of the proximal interventricular septum with that thickness is greater than 1.3cm or the thickness is grater 1.5 times than adjacent mid interventricular septum in apical 3 chamber view. Result: The incidence of patient with sigmoid septum(septum patient) is 24.8% (male 8.4%, female 16.3%) . The incidence increases with age and is more common in women. The patients with sigmoid septum were compared with targeted groups, there was no significant difference in blood pressure between them, but in height and weight, the former were lower. (p<001, p<03). In the patients with sigmoid septum, weight and the surface area of a body have an opposite correlation with increase of age (p<03, r=-0.34) (p<04, r=-0.31) , but no decrease of height was found according to age increase (p=0.47, r=-0.11) The main reason patients with sigmoid septum ask echocardiolography and its clinical symptoms were abnormal results of previous electrocardiography and/or usual chest pain. Compared with control group, interventricular septal thickness is larger in septum patients (p<001). The thickness of interventricular septum located in subaortic left ventricular outflow tract is 1.6±0.3cm. There is another web like structure that continued from interventricualr septum to anterior free wall of left ventricle and the width is 1.6±0.4cm. A sign of insignificant obstruction due to left ventricular outflow was observed from three sigmoid septum patients. Conculusion: Septum patient is largely found in older age group and the body weight and body surface area are lower than control group. The body weight and body surface area are opposite correlation with age. So sigmoid septum is considered as structural change by weight reduction with aging. It is assumed that septum patients are related with asymptomatic ischemia because they have more abnormal ECG finding such as T wave inversion on precordial leads or standard leads, nonspecific ST-T change, LBBB etc. Sigmoid septum is not only proximal interventricular septal bludging but also another web like structure continuing to anterior wall of left ventricle.

랫드에서 l-muscone의 급성독성 및 아급성독성시험 연구

오승민,연제덕,남혜운,박대규,조명행,정규혁 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

l-Muscone is synthesized for use as substitutive material of musk which is the active ingredient of woohwangchungsimwon. The objective of this investigation was to evaluate the acute and subacute toxicity of l-muscone in rats. In oral acute toxicity test, SPF Sprague-Dawley male and female rats were gavaged with l-muscone of two doses(0, 5.0g/㎏). No dead animal and abnormal autopsy findings were found in control and treated group. Body weights were slightly decreased in both sexes of rats treated with 5.0g/㎏. Therefore, oral LD_50 of l-muscone was consider to be higher than 5.0g/㎏ in male and female rats. In intraperitoneal acute toxicity test, rats were injected intraperitoneally with dosages of 0, 1,000, 1,316, 1,732, 2,279 and 3,000 ㎎/㎏. Decreased body weights and motor activities were observed at high dose group. Intraperitoneal LD_50 of l-muscone were 1,920 ㎎/㎏ in male and female rats. In the subacute study. l-muscone was administrated orally to both sexes of rats for 4 weeks as several doses(0, 10, 100 and 1,000 ㎎/㎏). There were neither dead animals nor significant changes of body weights during the experimental period. In addition, no differences were found between control and treated groups in clinical signs, urinalysis, hematology, serum biochemical analysis, and other findings. Above data suggest that no observed adverse effect level of l-muscone in rats might be over 1,000 ㎎/㎏/day in this study.

乳房炎 乳汁에서 分離된 原因菌의 抗菌劑 感受性

崔民淳,金鍾冕,趙正坤,蔡孝錫,姜明大,宋熹鍾 全北大學校 附設 畜産開發硏究所 1987 畜産開發硏究報告 Vol.1 No.-

A total of 189 strains (Streptococcus agalactiae and non-Streptococcus agalactiae 110, Staphylococcus aureus 63, and E. coli 16) isolated from bovine mastitie milk samples by "The Connecticut Mastitis Control Program" in dairy herds were examined for drug susceptibility to 8 drugs and analysed the rate of multiple resistant patterns. 1. All of the isolates were resistant to antimicrobial agents such as penicillin (PC), streptomycin (SM), kanamycin(KM), carbenicillin (CA), lincomycin (LM), gentamicin (GM), and tetracycline (TC), singly or multiply. The isolates were more susceptible to CA (84.1%),PC (78.9%), and SM (78.4%) than the the other drugs such as GM (51.8%), KM (48.7%), TC (19.6%) and LM (19.0%) in order. 2. The multiple resistant isolates (96.3%) were more than those of singly resistant isolates (3.7%) and 58 different drug resistant patterns were observed. Among 7 singly resistant isolates, CP was the commonest. While among 182 multiple resistant isolates, CP-KM-LM-TC-GM, CP-LM-TC, CP-LM-TC-GM, PC-CP-SM-LM-TC-GM, CP-KM-LM-TC, and CP-LM patterns were frequently encounted. 3. The strains of streptococcus spp. were more susceptible to CA (90.1%) and SM (82.9%) than the other drugs such as KM (43.2%), GM (38.7%), LM (13.5%), CP (10.8%) and TC (10.8%). Among them CP-KM-LM-TC-CM pattern was frequently encounted. 4. The strains of Sta. aureus were more susceptible to PC (74.6%),CA (69.8%), SM (65.1%) and GM (63.5%) than the other drugs such as KM (46.0%), CP (22.2%), TC (15.9%) and LM (9.5%). Among them CP-LM-TC pattern was commonly encounted. 5. The strains of E. coli were more susceptible to CA (100%) and KM (75.0%) tnan TC (9.5%). But the other drugs such as PC, SM, GM, CP and LM were not. Among them PC-CP-SM-LM-TC-GM pattern was commonly encounted.