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이대원,박현우,진병래,정영호,박영목,강석권 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2
미생물 살충제로 사용되고 있는 B. thuringiensis를 모기유충방제에 적용하기 위한 시도는 B. thuringiensis가 수서생태계에서 부유하지 못하고 가라앉으며, 생활환을 이루지 못한다는 문제점으로 인해 적용에 어려움이 있다. 따라서 본 연구는 모기유충에 강한 독성을 갖는 B. thuringiensis subsp. morrisoni PG-14의 cryIVD 유전자를 모기유충의 먹이인 cyanobactrium에 도입하기 위하여 발현벡터 pCYASK 5-1을 제작하고, cyanobacterium Synechocystis PCC6803에 형질전환시켜, 세포내에서 cryIVD 유전자의 발현과 뇌염모기에 대한 독성을 조사하였다. 그 결과 형질전환체 내에서 cryIVD 발현은 immunoblot 분석을 통해 B. thuringiensis subsp. morrisoni PG-14에서 발현된 단백질과 같은 분자량으로 발현되는 것을 확인하였으며, 모기유충에 대한 형질전환체의 독성은 1.40×10^6 cells/ml 농도에서 약 80%의 치사율을 나타내었다. Bacillus thuringiensis subsp. morrisoni PG-14 is a gram-positive soil bacterium producing mosquitocidal parasporal inclusions composed of several crystal proteins. Among these crystal protein genes, cryIVD gene is one of major component which has 72 kDa in size. However, these parasporal inclusions sink quickly from the surface of water where mosquito larval feeding occurred. To develope mosquitocidal cyanobacterium, therefore, we constructed the expression vector, pCYASK 5-1 harboring cryIVD gene. The expression vector, pCYASk5-1 was transformed into the cyanobacterium Synechocystis PCC6803 reported as a natural mosquito larval food source and the transformants were selected with kanamycin. Expression of cryIVD gene in transformant was characterized by SDS-polyacrylamide gel electrophoresis (PAGE) and immunoblot analysis. The mosquitocidal activity of a transformant was determined electrophoresis (PAGE) and immunoblot analysis. The mosquitocidal activity of a transformant was determined with Culex tritaeniorhynchus. The results showed that the transformed cyanobacterium is toxic to mosquito larvae and will be expected as a potential agent that is used for mosquito control.
RAPD(Random Amplified Polymorphic DNA)법을 이용한 한약재의 판별 연구
김대원,김도균,안선경,조동욱 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1
Conventionally, identification and classification methods of natural products include the morphological survey and assay of chemical disposition. Using these methods, however, is not satisfying for the precise identification of natural products because they are often variable in the compositions, genomic DNA analysis such as RAPD, RFLP and Amp-FLP can be adopted for this purpose. In this study, various ginsengs and bear gall bladder were tested for the development of genetic identification and classification method. Varieties of ginsengs such as, P. ginseng, P. quinquefolium, P. japonicus and P. notohinseng, were genetically analyzed by RAPD. Also, DNA isolated form Bear blood and gall bladder, Ursus thibetanus, Ursus americanus and Ursus arctors, were analyzed by the same method. The results demonstrated that the identification and classification of bear gall bladder and various ginsengs were possible by RAPD analysis. Therefore, this method was thought to be used as a additional method for the identification of other natural products.
최대석,권중장,최재원 경성대학교 공학기술연구소 2002 공학기술연구지 Vol.9 No.-
네트워크 응용 프로그램을 개발하기 위해서는 Socket API를 많이 이용한다. Socket API를 이용해 정상적인 동작을 하는 것은 아주 쉽다. 그러나 실제 개발 과정에서 정상적인 상황이 아닌 여러 가지 비정상적인 오류에 처하게 됨에 따라 속수무책일 때가 많다. 따라서 본 논문에서 각 플랫폼별로 비정상적인 오류들의 사례 연구를 통하여 효율적인 응용시스템 개발에 도움을 주고자 한다.
강대성,백원기 대진대학교 2002 大眞論叢 Vol.10 No.-
This study was to establish the floristic composition of resources vascular plants of the southern slope area at Mt. Wangbang(736.3 m; 127˚ 08' 11" -127˚ 12' 19" E, 3 7˚ 51' 29" ~37˚ 55' 32" N). This work was investigated during the period of 1996 & 2002. Resources vascular plants were composed of 100 families, 301 genera, 409 species, 47 varieties and 9 formae, Totaling 465 taxa. Among the investigated resources plants, 7 taxa(l.2%) were Korean endemic species. The special plants based on floral region by Ministry of Environment(1999) were 27 taxa; Ⅳ rank species 2 taxa, Ⅲ rank species 4 taxa, Ⅱ rank species 4 taxa, Ⅰrank species 17 taxa. A naturalized plants were 44 species, correspond to 20.2% of totaling 218 species appeared in South Korea. Life form spectra was H-D₄-R_(5)-e type and, useful resources plants are as follows; edible source(45.2%), pasture source (41.9%), medicinal source(34.0%) and ornamental source(l6.1%) in the investigated region.
尹大遠,朴鍾演,張牧淳,朴贊元 江原大學校 産業技術硏究所 1993 産業技術硏究 Vol.13 No.-
To analyze dynamic characterics for a motorcar, the creating and the hammering test methods are introduced. This paper has studied the method of computing the natural frequency of the impulse wave on a tire, the decay constant which determines the absorption capability for the impulsive force. Moreover we have examined the reliability for the decay constant calculated, in this paper theoritical results are agreed with the simulated ones.
이대원,김채웅 성균관대학교 생명공학연구소 1999 生命工學硏究 Vol.5 No.1
A hybrid environmental control system was designed and built in the multi-greenhouses. After the hardware system was developed, the software was designed and constructed for this project. A software was adequate for reading data from many sensors and operating side windows, top windows, curtains and fans in the two greenhouses. Separate programs for control of the hardware, written Visual basic, Turbo-C and Assembler computer language, were developed to make the hybrid environmental control system operate. The program was a menu driven program within Windows environment. The initial menu was easily implemented by pressing a keypad key(KPK) on the keyboard or a mouse. Continued manipulation of the KPK or a mouse enable the user to reach easily any of the sub modes in the many modes. This software proved to be a reliable program for the hybrid environmental control system in the two greenhouse.
이대원,김현태,이용국 성균관대학교 생명공학연구소 2001 生命工學硏究 Vol.7 No.1
Most of fruits need laboring greatly to harvest fruit in the period of harvest season. Grape also needs requiring a lot of labor to harvest in time, since harvesting of grape is cut and graped by hand. At abroad, especially, a harvester of grape, which is not eat freshly raw grape fruit but is processed to be made wine, was developed in France. However, a harvester of grape which can eat freshly raw grape fruit haven't not been developed. Therefore, this study was designed and constructed to develope an end effector of the robotic harvesting system. In order to find average and standard deviation of size and shape of a bunch grape, the grape farm was visited and investigated to find out the important factors for end effector to be designed and built. The efficient end-effecter should reach very well a bunch of grape without touching obstacles, such as leaf and stem in the grape vine. Also, it was made as simple, small and light as possible. Therefore, It was made of duralumin, which was lighter material than iron, to reach easily fresh grape fruit and reduce a torque. After finding the optimum initial speed, slew speed, ramp speed, the end effector proved to be a smooth operating end effector. For verifying efficiency of the end-effecter, the control board was designed and manufactured, and also developed the software to control the motors of the end effector.
집토끼 동방결절의 활동전압에 미치는 Vanadate의 효과
조용문,방효원,엄대용,이상돈 중앙대학교 의과대학 의과학연구소 1984 中央醫大誌 Vol.9 No.3
In order to investigate the electrphysiological effect of vanadate, a potent inhibitor of Na^+-K^+ pump, on the cardiac muscle, the alteration of transmembrane potential as influenced by the drug was measured in rabbit SA rodes in vitro by means of KCI glass intracellular microelectrodes. The followings were results of findings: 1. The rate of spontaneous firing was decreased in the presence of vanadate and the maximum inhibition which was about 50% of contral level was obtained at drug concentrations of 10^-3 M or higher. 2. The maximum diastolic potential(M.D.P.) was depressed by vanadate in a dose-dependent manner and the maximum inhibitory effect was obtained at the drug concentration of 10^-3 M. However the peripheral pacemaker cells were more resistant to the drug effect than the central pacemaker cells at lower concentrations of the drug. 3. In both central and perepheral pacemaker cells, vanadate had no effect on the M.D.P. in concentrations up to 5×10^-4 M. However on at 5×10^-2 M, the drug depressed the M.D.P. in the peripheral cells. 4. Changes of the action potential induced by vanadate were qualitatively similar to those occuring when the calcium concentration of the bathing medium was raised to 4mM. These results may be interpreted as that vanadate increases the rate of Ca^++ influx info the cell in S-A nodes.
위 유문동 평활근세포의 Caffeine 활성화 전류에 미치는 Ascorbate의 효과
이무열,방효원,엄대용,이상돈 중앙대학교 의과대학 의과학연구소 1992 中央醫大誌 Vol.17 No.4
In order to observe the effects of ascorbate on the background current in pyloric circular smooth muscle cells, caffeine of which action was fairly clarified, was used to induce the current systems. To block all of the known currents, various blocking agents were added in the external bathing solution. A part of pyloric circular muscle tissue was excised out, and enzymatic isolation technique was used to obtain Ca^2+ -tolerant single circular muscle cells. The whole cell mode of patch-clamp experiment was conducted. The results were as follows : 1. Ascorbate induced the increase of inward currents in the hyperpolarized potential ranges, and quite a large increase of outward currents in the depolarized ranges. 2. Caffeine also induced similar results, but the magnitude of outward current was smaller than that of assorbate in the depolarized ranges. 3. These phenomena were disappeared by addition of the anion channel blocker. 4. Replacement of external NaCl with NMG·Cl was rarely affected the effects of ascorbate and and caffeine. But the effects of ascorbate and caffeine were almost abolished when sxternal Cl^- was substituted for aspartate. These results suggested that ascorbate affected chloride current which thought as a component of the background currents in the pyloric circular smooth muscle cells.
李鎔國,李大源,黃憲,吳永鎭 成均館大學校 科學技術硏究所 1992 論文集 Vol.43 No.2
Planting, transplanting, and harvesting are important processes for the successful production of farm products in Korea because those require the short term high labor intensity. In the automatic production system of the field or the green house, planting is the first process required to be automated considering subsequent operations such as transpalnting and harvesting. A proto type experimintal robotized seeder was designed and built to handle various sizes and shape of seeds. The developed seeder is automatically controlled with one chip micro-controller. System is composed of the transfer arm, pecker, and the equipments integrated with sensor such as hopper, feeder, counter, and shutter. System allows to control the number of seeds to be planted. Results of the developed experimental robotized seeder are as following : 1. For the adequate feeding and counting of seeds, components such as hopper, feeder, counter, and shutter were designed and built. 2. Kinematic motion of the transfer arm was analyzed and designed. 3. Picker was designed to dig and drop seeds into the soil by the open and close action of the bottom gate controlled by DC solenoid. Digging depth of the pecker was controlled by the transfer arm.