RT-LAMP를 이용한 콩황화일반모자이크바이러스의 진단

배대현, 이영훈, 김봉섭, 윤영남, 강범규, 최만수, 구성철, 김현태, 윤홍태, 이수헌, 백인열 忠北大學校 農業科學硏究所 2014 農業科學硏究 Vol.30 No.2

Soybean yellow common mosaic virus (SYCMV) has been recently reported, it has been occurred a lot with Soybean mosaic virus (SMV) and Soybean yellow mottle mosaic virus (SYMMV) in soybean field. SYCMV belongs to genus of Sobemovirus and induced viral symptoms with yellowing, mottle and mosaic. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) method allowed one-step detection of gene amplification by simple procedure and needed only a simple incubator for isothermal template. This RT-LAMP method allowed direct detection of RNA from virus-infected plants without thermal cycling and gel electrophoresis. In this study, we designed RT-LAMP primers named SYCML-F3/B3/FIP/BIP from coat protein gene sequence of SYCMV. After the reaction of RTLAMP, products were identified by electrophoresis and with the detective fluorescent dye, SYBR Green I. under daylight and UV light. Optimal reaction condition was at 63 for 60min and the primers of RTLAMP showed the specificity for only SYCMV tested in this study.

AO Synthes 금속판을 이용한 전방 경추 유합술후 발생한 지연성 식도 천공

송하헌,김태균,심대무 圓光大學校 醫科學硏究所 1999 圓光醫科學 Vol.15 No.1

Esophageal perforation after anterior cervical discectomy operation has a rare incidence. Also Delayed Esophageal perforation after anterior cervical discectomy has a very rare incidence. Esophageal perforaton is usually characterized as being due to the surgical exposure, metallic implant, bone graft, fractured vertebrae and polymethylmethacrylate. Esophageal perforaton result in fatal mediastinitis, which may lead to die from sepsis. The mortality rate continues to be more than 20%. Factors that adversely affect the outcome include the location and severity of the esophageal injury] associated injuries and delayed diagnosis and treatment. The purpose of this report is to present the our case of a patient who had delayed esophageal perforation by irritation of metallic screw and 3 weeks post operatively, and to know how can we get a early diagnosis and treatment in delayed esophageal perforation.

損害賠償訴訟에 있어서의 證明度

裵大憲 忠南大學校 法學硏究所 1992 法學硏究 Vol.3 No.1

실질적인 원격교육 시스템과 그 원형 구축에 관한 연구

박대훈,남중헌,배재학 울산대학교 1999 공학연구논문집 Vol.30 No.2

본 논문의 주제는 실질적인 원격 교육 시스템의 설계와 그 원형 구축에 관한 연구이다. 원격 교육 시스템에 관한 다양한 장점이 있지만 그것을 설계하고 구현하는 일선교사들에게는 전문성의 부족으로 어려운 실정이다. 본 논문에서는 Lotus Domino/Notes에서 제공하는 웹 페이지 설계 기능과 native 웹 개발 도구들을 비교하고, 그것이 일선교사들에게 원격 교육 시스템을 설계하고, 관리하는데 얼마나 용이한가를 연구하였다. This paper is concerned with the design and implementation of a practical distance learning system. Even though there are various advantages of distance learning systems, it is too difficult for the teachers to design and implement them. In this paper, the followings are studied: (1) comparing native web publishing tools with the web page design facilities which Lotus Domino/Notes provides, and (2) how much easier it will be with the facilities for teachers to design and manage a distance learning system.

각-관 열교환기의 냉각능력 향상에 관한 연구

이대희,조헌노,원세열,이영민 인제대학교 1999 仁濟論叢 Vol.14 No.3

본 연구에서는 변압기 냉각용 각-관(Shell and Tube) 열교환기의 성능을 실험적으로 테스트하여 열교환기의 냉각능력을 향상시키는 기술을 개발하고자 하였다. 예열수조 2개를 항온조와 함께 사용함으로써 열교환기 입구에서와 온수의 초기온도를 60℃로 유지하고, cooling tower를 이용하여 입구에서 냉각수 온도를 20℃로 유지하였다. 냉각수 유량변화(5ℓ/min, 10ℓ/min, 15ℓ/min)에 따른 baffle의 형태(Single-segmental baffle, Double-segmental baffle, Disk & Doughnut baffle, Helical baffle)와 baffle의 개수(7개에서 5개) 및 tube의 개수(26개에서 18개)를 변화시켰을 때 열교환기의 냉각능력을 각각 실험하였다. 온수와 냉각수의 입ㆍ출구에서의 온도변화를 측정하기 위하여 Thermocouple을 온수와 냉각수 관의 입ㆍ출구에 하나씩 설치하였다. 실험결과 Disk & Doughnut, Helical baffle, Double-segmental baffle의 열전달률이 Single-segmental baffle보다 5.3%, 5.5%, 11.8% 높고, Double-segmental baffle의 경우 Tube 수의 감소율이 35%일 때 열전달률의 감소율은 14.3%임을 알 수 있었고, baffle의 수가 5개에서 7개로 증가했을 때 열전달률이 12.1% 증가함을 알았다. An experimental study to enhance the cooling capacity of the shell and tube type heat exchanger has been carried out. The initial temperature of hot water in the heat exchanger is maintained at 60℃, using two preheating water baths and one constant temperature water bath, while the coolant temperature is maintained at 20℃ by way of the cooling tower. For the coolant rates of 5 ℓ/min, 10 ℓ/min, 15 ℓ/min, the experiments to determine the heat exchanger cooling capacity have been performed using the various types of baffle(Single- segmental baffle, Double-segmental baffle, Disk & Doughnut baffle, Helical baffle), the number of baffles(from 7 to 5) and number of tubes(from 26 to 18). Thermocouples are installed in the inlet and outlet of hot-water and coolant tube to measure the temperature difference. It was found that the heat transfer rates for Disk & Doughnut baffle, Helical baffle, Double-segmental baffle are 5.3%, 5.5%, 11.8% higher than the Single-segmental baffle, respectively. When the number of tube is reduce by 35%, the 14.3% decrease in the heat transfer rate occurs, while when the number of baffle increases from 5 to 7, the 12% increase in the heat transfer rate occurs.

정부조직 개편에 따른 전자서명의 통합 및 관련법의 개정 논의

배대헌 한국정보사회진흥원 2008 정보화정책 Vol.15 No.4

2008년 2월 정부조직법이 개정되면서 종래의 전자서명법상 공인전자서명과 전자정부법상 행정전자서명의 운영체계가 모두 행정안전부의 소관 사무 아래에 놓이게 됨에 따라 이를 아우르는 새로운 체제를 새롭게 꾸려야 하는 여건을 맞이하게 되었다. 각각 다른 입법 취지에 따라 '성장'한 전자서명은 외부적 요인에 의하여 새로운 논의를 불러오게 되었고, 이로써 '무르익게' 하는 조건·상태를 고려하는 전환기를 맞이하고 있다. 이 글은 전자서명에 관한 그간의 법률 제정 및 개정에 관한 내용을 개관한 후, 현행 2원적 전자서명체계의 통합 등에 관한 인증정책 논의내용 및 입법 예고된 정보시스템 등 기반보호에 관한 법률(안)의 전자서명 관련 규정내용을 살펴보았다. 전자서명은 정보통신기반(또는 정보시스템 등의 정보기반)과 서로 묶어 하나의 법률 하에 규율되는 것이 합당하지 아니하다는 점과 공인전자서명과 행정전자서명을 병렬체계로 구성하여 운용하려는 법률(안)에 대하여 필자의 견해를 정리하였다. This article is intended to provide the assessment on revised Draft of Electronic Signature Act(hereinafter 'Draft'). The Draft covers two areas, which are of the protection of information-telecommunication infrastructure, and of the electronic signatures. While the former is mainly based on the hardware for the protection against the intruder on Internet, the latter has designed not only to facilitate the electronic transactions, but also to cover their concerns of how legally binding the transactions are. These subject-matters are heterogeneous, hard to be tied up titled Act as the current Draft. It is necessary to prescribe the electronic signatures under a newly titled Combined Electronic Signature Act which have realm extended the private to the public, that is, it is to get together the electronic signature under the Electronic Signature Act and the electronic seal(or administratively electronic signature) under the Electronic Government Act. And with respect to the relationship between electronic signature and electronic seal under a newly titled Combined Electronic Signature Act, this article recommends that the other two signatures keep up with each its own system based on Public Key Infrastructure(PKI) until two systems can work the compatibility perfectly.

만성 간질환에 대한 DDB(Biphenyl Dimethyl Dicaboxylate)의 임상적 효과

이헌영,임의혁,김성걸,김진희,육은주,성자원,김병호,정형용,강대영,김영건 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.2

To investigate the effects of short-term administration of DDB(Biphenyl Dimethyl Dicarboxylate) for decreasing elevated serum aminotransferase levels in chronic liver diseases, authors applied 3 capsules of DDB per day at each meal time for 35 patients and compared with 35 patients treated with other conservative management. The following results were obtained. 1. Serum alanine aminotransferase (ALT) levels significantly decreased from 247.1± 180.0 IU/L of pretreatment level to 96.6±126.0 IU/L, 61.1±62.7 IU/L and 38.0±26.8 IU/L after 1, 3 and 6 months of treatment(p<0.05) and effective rates were observed in 60.0%, 71.4% and 88.6% of patients after 1, 3 and 6 months of treatment. 2. Serum aspartate aminotransferase (AST) levels decreased also significantly from 167.8±96.9 IU/L of pretreatment levels to 124.4±155.0 IU/L, 77.9±49.6 IU/L, 60.6± 46.9 IU/L after 1, 3 and 6 months of treatment(p<0.05), but decreasing pattern of serum AST was less significant than that of serum ALT. 3. No significant difference were observed in the serum ALT and AST changes according to the cause and duration of hepatitis. 4. No significant adverse effects were observed in whole patients treated with DDB. It is suggested that small dose administration of DDB is effective for decreasing serum aminotrans ferase levels in chronic liver disease in which other conservative management was not effective.

광도파로형 표면 플라즈몬 공명 센서 소자의 제작 및 그 특성

조동헌,엄년식,장수원,권대혁,강신원 경북대학교 센서기술연구소 2002 센서技術學術大會論文集 Vol.13 No.1

독일 루어광산업에서의 노동시장의 발전과 노동시장 당사자들의 대응(1900~1933)

이대헌 전남사학회 2003 역사학연구 Vol.20 No.-

This article outlines the development of the labor market in the Ruhr coal mining area from the late 1890s to the early 1930s years, analyses the measures of the parties concerned(industrialists and workers or trade unions), and estimates the efficiency of their actions. The Ruhr coal mining area had suffered from labor shortage before 1924 when it began to experience labor redundancy. The responses of the parties concerned to the fluctuation of the labor market were very various. In the time of the labor shortage the employers in the Ruhr coal mining tried to mobilize and keep the workers in their working place by providing incentives. For example, before 1914 the industrialists introduced the company welfare schemes including the housing program for workers. But the plan of the industrialists brought out a little or no result, because the mining workers increasingly were not reluctant to leave the working place for the others. In the period of the World War(1914~1918) and the post-war-years from 1919 to 1923 the mining labor market was subject to fundamental change with the alternation of the economy between war time and peace time. As the labor shortage continued the state intervention and the institutionalization of labor market system tended to be reinforced. The French and Belgian occupation of the Ruhr coal mining area from January to September 1923 marked a turning point in the Ruhr labor market. When the ?passiver Widerstand? ended, the financial situation of the coal mining companies in the Ruhr was the worst and their products were dull of sale. In order to overcome the crisis in coal mining industry the industrialists in the Ruhr attempted to rationalize the mining industry. The measures of industrial rationalization in the Ruhr coal mining industry included shutdown in full or in part and modernization or replacement of the old facilities. The consequences of the rationalization were mass layoffs of miners. Therefore it can be said that the rationalization of the Ruhr coal mining companies not only meant an active management policy but also a very effective labor market policy. Now the employers had a free hand to decide who would be redundant or not. Inevitably it intensified the conflicts between the employees and employers in Ruhr particularly in the time of the world-wide economic recession from 1930 to 1933. In sum, the change of labor market in the Ruhr coal mining industry since 1924 contributed to transfer power and influence to entrepreneurs in the Ruhr.

19세기 말 20세기 초 독일 루어지역 광산업에서 <노동자 위원회>의 도입을 둘러싼 논의

이대헌 한국서양사학회 2004 西洋史論 Vol.0 No.80

This paper intends to examine the institutionalization of the workers committee in German mining industry. The first attempt to institutionalize the industrial conflicts had something to do with the great strike of the Ruhr mining workers in 1889. The miner strike of 1905 was the most important moment to force the obligatory establishment of the workers committee. But in the frist election for the workers committees in 1905 did the absolute majority of the Ruhr miners abstain in order to protest against the unreasonable provisions of the mining law. Most studies about the workers committees had taken the abstention as the propaganda of the social democratic "alter Verband". But this paper had come to the conclusion that there was a close relation between the abstention and the organizational endeavors of trade unions. The prussian government intended to check the growth of labor movement by establishing the workers committee system. But the coal mining industrialists would be willing to make no concession at all to the workers committee; the trade unions would like to use it for their organizations. Neither workers nor employers had trusted each other. The workers committees in the mining industry were established to substitute this mistrust by the mutual regulation trust. But in this early phase they could neither function well nor contribute to the solution of conflicts in work places because of the legal flaws.