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Da-Ran Kim,Youn-Sig Kwak 한국농약과학회 2021 한국농약과학회 학술발표대회 논문집 Vol.2021 No.11
The strawberry is one of the major economic crops in Korea. Gray mold disease which caused by the Botrytis cinerea and Blossom blight disease occurred by Cladosporium cladosporioides which are known to be the most problematic for strawberry anthosphere. In previously study, Streptomyces globisporus SP6C4 was isolated at strawberry anthosphere and suggested a majority antifungal compound was conprimycin and grisin. The S. globisporus SP6C4 has study of antifungal compound, which metabolite to be novel compound. These compound is secondary metabolite, which is not necessary but do adapt ecology system with some metabolite. In a previous survey from 2017 to 2018, strawberry cultivation of the plant decreased Gray mold and Blossom blight disease incidence and severity. In this study, control efficacy of S. globisporus SP6C4 was evaluated on the two different disease of strawberry. Two of different disease of B. cinerea and C. cladosporioides were decrease to the disease severity, also more control the mummification value on fruits. In most things, anthosphere diseases were the most important to decrease the disease severity to improve productivity in cultivation system.
Da-Ran Kim,이수인,곽연식 한국식물병리학회 2023 Plant Pathology Journal Vol.39 No.6
The aim of this study was to investigate the regulation of lantipeptide production in Streptomyces globisporus SP6C4, which produces the novel antifungal lantipeptides conprimycin and grisin, and to identify the role of cytochrome P450 (P450) in tis regulation. To investigate the regulation of lantipeptide production, we created gene deletion mutants, including ΔP450, ΔtsrD, ΔlanM, ΔP450ΔtsrD, and ΔP450ΔlanM. These mutants were characterized in terms of their morphology, sporulation, attachment, and antifungal activity against Fusarium oxysporum. The gene deletion mutants showed distinct characteristics compared to the wild-type strain. Among them, the ΔP450ΔlanM double mutant exhibited a recovery of antifungal activity against F. oxysporum, indicating that P450 plays a significant role in regulating lantipeptide production in S. globisporus SP6C4. Our findings highlight the significant role of P450 in the regulation of lantipeptide production and morphological processes in S. globisporus. The results suggest a potential link between P450-mediated metabolic pathways and the regulation of growth and secondary metabolism in SP6C4, thereby highlighting P450 as a putative target for the development of new antifungal agents.
Da Li,Ikjae Lee,Cong Yi,Wei Gao,Chunhui Song,Shenglei Fu,Moohyun Kim,Alex Ran,Tuanjie Liu Techno-Press 2023 Ocean systems engineering Vol.13 No.3
The global performance of a 15 MW floating offshore wind turbine, a newly designed semisubmersible floating foundation with multiple heave plates by CNOOC, is investigated with two independent turbine-floater-mooring coupled dynamic analysis programs CHARM3D-FAST and OrcaFlex. The semisubmersible platform hosts IEA 15 MW reference wind turbine modulated for VolturnUS-S and hybrid type (chain-wire-chain with clumps) 3×2 mooring lines targeting the water depth of 100 m. The numerical free-decay simulation results are compared with physical experiments with 1:64 scaled model in 3D wave basin, from which appropriate drag coefficients for heave plates were estimated. The tuned numerical simulation tools were then used for the feasibility and global performance analysis of the FOWT considering the 50-yr-storm condition and maximum operational condition. The effect of tower flexibility was investigated by comparing tower-base fore-aft bending moment and nacelle translational accelerations. It is found that the tower-base bending moment and nacelle accelerations can be appreciably increased due to the tower flexibility.
Involvement of senescence marker protein-30 (SMP30) on the Smad3-dependent hepatic injury
Da-Hee Jeong,Sun-Hee Do,Hai-Jie Yang,Dong-Wei Yuan,Jin-Kyu Park,Il-Hwa Hong,Moon-Jung Goo,Hye-Rim Lee,Mi-Ran Ki,Ho-Yong Park,Kwang-Hee Son,Dong-Ha Sin,Hae-Young Chung,Akihito Ishigami,Kyu-Shik Jeong 한국생명과학회 2006 한국생명과학회 심포지움 Vol.47 No.-
A Genome-Wide Analysis of Antibiotic Producing Genes in Streptomyces globisporus SP6C4
Da-Ran Kim,Youn-Sig Kwak 한국식물병리학회 2021 Plant Pathology Journal Vol.37 No.4
Soil is the major source of plant-associated microbes. Several fungal and bacterial species live within plant tissues. Actinomycetes are well known for producing a variety of antibiotics, and they contribute to improving plant health. In our previous report, Streptomyces glo- bisporus SP6C4 colonized plant tissues and was able to move to other tissues from the initially colonized ones. This strain has excellent antifungal and antibacterial activities and provides a suppressive effect upon various plant diseases. Here, we report the genome-wide analysis of antibiotic producing genes in S. globisporus SP6C4. A total of 15 secondary metabolite biosynthetic gene clusters were predicted using antiSMASH. We used the CRISPR/Cas9 mutagenesis system, and each biosynthetic gene was predicted via protein basic local alignment search tool (BLAST) and rapid annotation using subsystems technology (RAST) server. Three gene clusters were shown to exhibit antifungal or anti- bacterial activity, viz. cluster 16 (lasso peptide), cluster 17 (thiopeptide-lantipeptide), and cluster 20 (lanti- peptide). The results of the current study showed that SP6C4 has a variety of antimicrobial activities, and this strain is beneficial in agriculture.