http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Simple Sequence Repeat Primer에 의한 딸기의 Genomic DNA 분석
양덕춘,최성민,김무성,민병훈 경희대학교 생명자원과학연구원 2002 硏究論文集 Vol.22 No.-
본 연구는 진핵세포에 널리 산재되어있는 repeat sequence를 primer로 사용하여 딸기의repeat sequence 좌위를 증폭시켜서 분석하고 이를 같은 종일지라도 지역간의 차이로 인한 환경의 변화 또는 재배방법의 차이에 의해서 일어나는 변이를 분석하는 방법에 활용할 수 있도록 하기 위하여 수행하였다. GGC repeat sequence의 딸기 genomic DNA에 존재여부를 판명하기 위하여 (GGC)_(6) probe를 DIG을 사용하여 labeling하여 딸기 genomic DNA에 hybridization한 결과 확실한 spot이 나타났다. 또한 repeat sequence로 이루어진 UBC SSR primer 100개중에서 4가지 서로 다른 primer에서 선명한 band를 확인할 수 있어서 딸기의 genomic DNA내에 여러 종류의 repeat sequence가 있는 것으로 판단되었다. 따라서 각 품종별 표지로서 이용성을 알아보고자 PCR을 수행하였는 바 (ACC)_(6)와 (CCG)_(6)의 primer에서는 다른 band에 비해 매우 확실한 밴드를 형성하였다 이러한 PCR product의 염기서열분석을 위해 우선 대량증식을 위한 PCR의 적정 조건은 primer 10p㏖, DNA 50ng으로서 수행하는 것이 DNA의 증폭에 좋았다. 증폭된 1 Kb DNA 절편을 이용하여 염기서열을 조사하였던 바, 808bp의 서열을 알아냈고 그 반복염기서열은 효모와 사람에 존재하는 유전자이었으며 Cdontella sinensis와의 유의성 검정결과 ACC 반복염기에 62% 동일성을 나타내었다. This study was carried out to investigate genetic variation caused by environmental change or cultural method difference, which was performed by amplifying repeat sequence loci of strawberry widely dispersed in eukaryotic cell. DIG labeled(GGC)_(6), probe was used to test if GGC repeat sequence exists in strawberry genomic DNA, resulting in clear spot on the filter. Among the 100 UBC SSR primers which contain repeat sequence, four different primers amplified clear DNA fragments, suggesting that the repeat sequence exist in the genome of strawberry. PCR was also performed to test whether these primers could be used for a marker among strawberry cultivars. (ACC)_(6), (No. 861) and (CCG)_(6) (No. 865) primers showed very clear bands compared to the other primers. The amplified 808 bp of 1 kb DNA was sequenced, and database search using the sequence information showed that the repeat sequence was the gene existed both in yeast and human, and a significant sequence homology to Odontella sinensis was found.
정재훈,양덕춘 경희대학교 유전공학연구소 1990 遺傳工學論文集 Vol.2 No.-
These studies were conducted to control of apple cankers caused by Valsa ceratosperma strains by treatment of chemical compounds, antibiotics, extracts of herbal plants and compounds produced from mutant of Streptomyces melanosporofaciens. Effects of chemical control for apple canker were evaluated by growth inhibition of Valsa ceratosperma on the PDA media and the most effective chemical was found to be berberine followed by neoasogin, branchi Q, in general. Inhibitory concentration of Valsa ceratosperma was 500㎎/ℓ in berberine, and 10,000㎎/ℓin neoasogin. The antifungal antibiotic, cycloheximide, was effective for growth inhibition of Valsa ceratosperma; all strains were inhibited on the media with 100㎍/㎖ of cycloheximide and M26 strain was inhibited by lower concentration of 10㎍/㎖. Effects of extract of herbal plants for growth inhibition of Valsa ceratosperma were excellent with Phellodendron amurense and Coptis japonica, and especially in Coptisjaponica, low concentration of 1,000㎎/ℓ was effective Compounds produced from mutant of S melanosporofaciens for growth inhibition of Valsa ceratosperma were more effective than other compounds, i.e., chemical compounds, antibiotics and extracts of herbal plants. Minimum inhibitory concentration of compound C-1 for IF03025 and M1099 was 3125㎍/㎖.
김덕한,송화영,이동훈 서울産業大學校 2005 논문집 Vol.54 No.1
This paper introduces a study for the noise reduction design of a range hood for household. Generally, range hoods have a built-in sirocco fan from which squawky noises are generated. Though the squawky noises have low noise level, these kinds of noises make most of the users nervous. For the purpose of noise reduction, in this study, a perforated panel system is installed in the fan housing of range hood. From the experimental results, it is confirmed that the noise level emitted from the range hood is decreased above 2dBA in all frequency regions.
정재훈,양덕춘 경희대학교 부설 식량자원개발연구소 1990 硏究論文集 Vol.11 No.-
These studies were conducted to control of apple cankers caused by Valsa ceratosperma strains by treatment of chemical compounds, antibiotics, extracts of herbal plants and compounds produced from mutant of Streptomyces melanosporofaciens. Effects of chemical control for apple canker were evaluated by grwoth inhibition of Valsa ceratosperma on the PDA media and the most effective chemical was found to be berberine followed by neoasogin, branchi Q, in general. Inhibitory concentration of Valsa ceratosperma was 500mg/ℓ in berberine, and l0,000mg/ℓ in neoasogin. The antifungal antibiotic, cycloheximide, was effective for growth inhibition of Valsa ceratosperma; all strains were inhibited on the media with l00㎍/㎖ of cycloheximide and M26 strain was inhibited by lower concentration of 10㎍/㎖. Effects of extract of herbal plants for growth inhibition of Valsa ceratosperma were excellent with Phellodendron amurense and Coptis japonica, and especially in Coptis japonica, low concentration of l,000mg/ℓ was effective. Compounds produced from mutant of S. melanosporofaciens for growth inhibition of Valsa ceratosperma were more effective than other compounds, i.e., chemical compounds, antibiotics and extracts of herbal plants. Minimum inhibitory concentration of compound C-1 for IF03025 and M1099 was 31.25㎍/㎖.
이석훈,윤덕균 한국품질경영학회 2004 품질경영학회지 Vol.32 No.3
In this paper we propose a new method of evaluating customers' service quality using triangle fuzzy numbers and semantic differential scale. These two parameters are useful for quantifying the customers' linguistic variables and subjective judgements. We define the Service Quality(SQ) as the ratio of the expected and perceived service and address the Agreement Rate(AR) calculated from the hamming distance in order to define the Service Rate(SR) as the geometric mean of the SQ and AR. Using the SR in the analysis of service attributes provides more appropriate to measure the customers' service quality and to establish a creative service policy for competitive service quality improvement.
A Note on Basic Construction and Index for Subfactors
DEoK HooN Boo 충청수학회 1990 충청수학회지 Vol.3 No.1
The converse of Pimsner-Popa theorem is proved, and represent the index [M : N] by finite sum of elements of M.
Clinical Outcomes of Late Conversion to Once-Daily Tacrolimus after Liver Transplant
( Deok Gie Kim ),( Yoon Bin Jung ),( Jee Youn Lee ),( Jae Geun Lee ),( Sung Hoon Kim ),( Han Dai Hoon ),( Man Ki Ju ),( Gi Hong Choi ),( Jin Sub Choi ),( Myoung Soo Kim ),( Soon Il Kim ),( Dong Jin Jo 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1
Aims: Since a once-daily tacrolimus (TAC-OD) has been introduced in the field of transplantation, many studies reported advantages of the new drug, such as the better adherence and the less intrapatient variability than the twice-daily tacrolimus (TAC-TD). Recently, promising results were announced regarding better clinical outcomes of the early conversion to TAC-OD in liver transplant patients. In this study, we investigated clinical outcomes of late conversion to TAC-OD more than 6 months after transplantation. Methods: A total 281 patients who received liver transplant patient from January 2012 to January 2017 took TAC-TD from operation. Of them, 38 patients were converted to TAC_OD 6 months after transplantation, while others remained with TAC-TD. We compared graft survival and postoperative complication between two groups, especially with regard to biliary complication known as implication of chronic rejection. Results: Among the patients, 151 were living donor liver transplantation. There was no difference in demographics and operative characteristics between two groups. Biliary stricture occurred later after 6 month after transplantation was 18 (7.4 %) in TAC-TD and 2 (5.3 %) in TAC-OD. The cumulative incidence was not statistically different. However, overall graft survival rate was significantly higher in TAC-OD than that in TAC-TD (P=0.049). Biliary stenosis free survival was also higher without significance (P=0.065). Adverse event of drug was similar between two groups. Conclusions: Late conversion to TAC-OD was safe and feasible. It has advantages of graft survival compared to TAC-TD.