인삼(Panax ginseng C.A. Meyer) 신품종 고풍의 육성경과 및 생육특성

권우생(Woo-Saeng Kwon),이장호(Jang-Ho Lee),박찬수(Chan-Soo Park),양덕춘(Deok-Chun Yang) 고려인삼학회 2003 Journal of Ginseng Research Vol.27 No.2

To develop a new ginseng variety with good quality and high yielding, a lot of individual ginseng plant were selected in the farmers' fields in 1968. Among them, a promising line, 680-83-4, has been developed through comparative cultivation of several lines selected with pure line separation from local races in KT&G Central Research Institute, preliminary and advanced yield trials were performed for 8 years. 680-83-4 was designated as KG103, which was then registered as a new variety “Gopoong” with the regional yield and adaptation trials for 10 years (1981-1990) on December<br/> 27, 2000 in Korea. Gopoong has different phenotype of a dark violet stem and dark red fruit and inverted triangle shape of berries cluster as compared with other varieties. Taproot of Gopoong was longer than local race Jakyungjong, and root yield was 4.5% higher than local race Jakyungjong. In red ginseng quality, the rates of Chun-Jeesam grade(Chun and Jee means 1st and 2nd grade, respectively) were 16.6% and 9.4% for Gopoong and Jakyungjong, respectively. In these results, it was that Gopoong was superior ginseng line with good quality for manufacture of red ginseng.

Simple Sequence Repeat Primer에 의한 딸기의 Genomic DNA 분석

양덕춘,최성민,김무성,민병훈 경희대학교 생명자원과학연구원 2002 硏究論文集 Vol.22 No.-

본 연구는 진핵세포에 널리 산재되어있는 repeat sequence를 primer로 사용하여 딸기의repeat sequence 좌위를 증폭시켜서 분석하고 이를 같은 종일지라도 지역간의 차이로 인한 환경의 변화 또는 재배방법의 차이에 의해서 일어나는 변이를 분석하는 방법에 활용할 수 있도록 하기 위하여 수행하였다. GGC repeat sequence의 딸기 genomic DNA에 존재여부를 판명하기 위하여 (GGC)_(6) probe를 DIG을 사용하여 labeling하여 딸기 genomic DNA에 hybridization한 결과 확실한 spot이 나타났다. 또한 repeat sequence로 이루어진 UBC SSR primer 100개중에서 4가지 서로 다른 primer에서 선명한 band를 확인할 수 있어서 딸기의 genomic DNA내에 여러 종류의 repeat sequence가 있는 것으로 판단되었다. 따라서 각 품종별 표지로서 이용성을 알아보고자 PCR을 수행하였는 바 (ACC)_(6)와 (CCG)_(6)의 primer에서는 다른 band에 비해 매우 확실한 밴드를 형성하였다 이러한 PCR product의 염기서열분석을 위해 우선 대량증식을 위한 PCR의 적정 조건은 primer 10p㏖, DNA 50ng으로서 수행하는 것이 DNA의 증폭에 좋았다. 증폭된 1 Kb DNA 절편을 이용하여 염기서열을 조사하였던 바, 808bp의 서열을 알아냈고 그 반복염기서열은 효모와 사람에 존재하는 유전자이었으며 Cdontella sinensis와의 유의성 검정결과 ACC 반복염기에 62% 동일성을 나타내었다. This study was carried out to investigate genetic variation caused by environmental change or cultural method difference, which was performed by amplifying repeat sequence loci of strawberry widely dispersed in eukaryotic cell. DIG labeled(GGC)_(6), probe was used to test if GGC repeat sequence exists in strawberry genomic DNA, resulting in clear spot on the filter. Among the 100 UBC SSR primers which contain repeat sequence, four different primers amplified clear DNA fragments, suggesting that the repeat sequence exist in the genome of strawberry. PCR was also performed to test whether these primers could be used for a marker among strawberry cultivars. (ACC)_(6), (No. 861) and (CCG)_(6) (No. 865) primers showed very clear bands compared to the other primers. The amplified 808 bp of 1 kb DNA was sequenced, and database search using the sequence information showed that the repeat sequence was the gene existed both in yeast and human, and a significant sequence homology to Odontella sinensis was found.

Ti-plasmid에 依하여 形質轉換된 煙草組織의 特性과 再分化 樣相

梁德春,李政明 경희대학교 부설 식량자원개발연구소 1987 硏究論文集 Vol.8 No.-

Studies were conducted to examine the characteristics of teratoma shoots obtained from diploid and haploid tobacco crown gall tumor tissues induced by the inoculation of Agrobacterium tumefaciens and the effects of plant hormones and active carbon in the cultural media on the growth and regeneration patterns of the teratoma shoots. Results can be summarized as follows. 1. Teratoma shoots could be obtained when the crown gall tumor, induced by the inoculation of A. tumefuciens strain C-58 to the stem, was successively grown on hormone-free medium. 2. The growth of tumor callus induced by C-58 strain was inhibited when several plant hormones were added to the cultural media. Plant regeneration could not be obtained from the tumor callus or the teratomas. 3. Addition of active carbon and benzyl adenine to cultural media was effective for shoot elongation and reduction in multiple shoot formation. 4. The growth of tumor callus obtained from the haploid plant was promoted by the addition of plant hormones to the media. Whole plants were also regenerated from the teratoma shoots formed from tumor callus. 5. Normal or transformed plants were regenerated from the tumor callus formed by the inoculation of a strain of A. tumefaciens collected from field soil. The transformed plants are characterized by having twisted dark-green leaves, shorter internodes, and abundant axillary shoots.

Randomly Amplified Polymorphic DNA를 이용한 인삼의 DNA 분석

양덕춘,김무성 한국국제농업개발학회 2002 韓國國際農業開發學會誌 Vol.14 No.4

본 연구는 인삼의 종 및 변종간의 유전적 다양성을 식물의 분류학적 연구에 이용하여 품종육성을 위한 기초자료로 활용하고자 인삼 조직의 DNA를 사용하여 RAPD primer를 선정하고 RAPD양상을 조사하고자 수행되었다. 인삼의 RAPD를 수행함에 있어서 DNA분리에 이용되는 조직의 차이가 PCR이후 DNA band 양상에 어떠한 영향을 미치는 지를 조사하고자 포장에서 정상적으로 생육하고 있는 자경종에서 조직별로 시료를 채취한 후 primer를 사용하여 PCR을 수행하여 나타난 band의 양상은 모든 조직에서 동일한 band를 형성하였다. 50개의 서로 다른 10-mer random primer를 이용하여 band가 확실히 나타나는 18개의 primer를 선발하여 자경종, 풍기황숙, 중국삼, 미국삼의 DNA를 사용하여 PCR 증폭을 하였다. 그러한 PCR product를 1.2%의 Agarose gel에 전기영동하여 banding patter에 따라 분석하였다. 이러한 RAPD 결과를 Cluster analysis 방법을 사용하여 similarity index(SI)로서 원연관계를 조사한 결과 자경종과 풍기황숙이 근연관계를 가졌고 중국삼은 자경종과 약간의 차이를 가졌으며 또한 자경종, 풍기황숙, 중국삼은 미국삼과 변이의 차이를 나타냈다. This study was carried out to analyse genetic variability among different ginseng cultivars, and to use it for the basic information on rearing ginseng cultivars. In performing RAPD of ginseng, DNA was extracted from the different tissues of 'Jakyung' growing normally in the field. PCR was performed to check if there is difference among different tissues, resulting in the same banding pattern, Eighteen of 50 different 10-mer UBC primers showing clear bands were selected, and PCR amplification was performed using DNA extracted from ginseng cultivars, Jakyung, Punggi Hwangsuk, China, and America. The PCR product amplified was analysed according to banding patter after electrophoresis in 1.2% agarose gel. Genetic difference based on RAPD result was investigated by similarity index (SI) of Cluster analysis. As a result, Jakyung and Punggi Hwangsuk had close relationship, whereas Chinese ginseng differed a little from Jakyung. Jakyung, Punggi Hwangsuk, and Chinese ginseng showed genetic difference from America ginseng.

Agrobacterium tumefaciens C58에 依하여 形質轉換된 人蔘組織에서의 saponin 生産

梁德春,李政明 경희대학교 부설 식량자원개발연구소 1988 硏究論文集 Vol.9 No.-

The present study was conducted to obtain some basic information on the transformation of ginseng tissue, identification of opine compound and protein, and saponin production from ginseng tissue transformed with pTiC58. Ginseng tumor callus induced by pTiC58 could be continuously cultured on the phytohormone-free basic medium. The transformation was reconfirmed by the detection and identification of opine compounds from the tumor callus. The transformed ginseng calli contained higher amount of protein than normal calli and the protein pattern of transformed calli was quite different from that of normal callus. Higher saponin content was found in the transformed callus than the normal callus, and ginsenoside composition of the transformed callus was similar to that of cultivated ginseng root, but quite different from that of normal callus.

다시마 푸코이단추출물과 지질대사의 연관성

강금석,남천석,김인덕,권륜희,이동찬,허예영,하배진 신라대학교 자연과학연구소 2007 自然科學論文集 Vol.16 No.-

The effects of Laminaria japonica fucoidan extract (LJFE) on the biochemical parameters of lipid-related function were investigated in liver and serum of CCl_(4)-treated rats. LJFE of 100 mg/kg concentration was intraperitoneally administered into rats at dose of 1.5 ml/kg for 14 days. On the day 15, 3.3 ml/kg of CCl_(4) dissolved in olive oil (1:1) was injected 12 hours before anesthetization. We examined the lipid-related functions and enzyme activitys by measuring the levels of total cholesterol (TC), triglyceride (TG), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) in serum. The results showed that CCl_(4) treatment increased the high values of TG, LDH and ALP decreased the low values of TC but LJFE pretreatment decreased the high values of TG, LDH and ALP to the low values, increased the TC. It was suggested that LJFE can be used as the potential candidate for the lipid-metabolism natural supplement.

사과나무 腐爛病 防除에 關한 硏究 : 腐爛病源菌의 生育抑制에 미치는 化學藥劑, 抗生劑 및 生藥抽出物의 效果 The effects of Chemical Compounds, Antibiotics and Extracts of Herbal Plants on the Growth Inhibition of Valsa ceratosperma Strains

정재훈,양덕춘 경희대학교 부설 식량자원개발연구소 1990 硏究論文集 Vol.11 No.-

These studies were conducted to control of apple cankers caused by Valsa ceratosperma strains by treatment of chemical compounds, antibiotics, extracts of herbal plants and compounds produced from mutant of Streptomyces melanosporofaciens. Effects of chemical control for apple canker were evaluated by grwoth inhibition of Valsa ceratosperma on the PDA media and the most effective chemical was found to be berberine followed by neoasogin, branchi Q, in general. Inhibitory concentration of Valsa ceratosperma was 500mg/ℓ in berberine, and l0,000mg/ℓ in neoasogin. The antifungal antibiotic, cycloheximide, was effective for growth inhibition of Valsa ceratosperma; all strains were inhibited on the media with l00㎍/㎖ of cycloheximide and M26 strain was inhibited by lower concentration of 10㎍/㎖. Effects of extract of herbal plants for growth inhibition of Valsa ceratosperma were excellent with Phellodendron amurense and Coptis japonica, and especially in Coptis japonica, low concentration of l,000mg/ℓ was effective. Compounds produced from mutant of S. melanosporofaciens for growth inhibition of Valsa ceratosperma were more effective than other compounds, i.e., chemical compounds, antibiotics and extracts of herbal plants. Minimum inhibitory concentration of compound C-1 for IF03025 and M1099 was 31.25㎍/㎖.

사과나무 腐爛病 防除에 關한 硏究 : 腐爛病源菌의 生育抑制에 미치는 化學藥劑, 抗生劑 및 生藥抽出物의 效果 The Effects of Chemical Compounds, Antibiotics and Extracts of Herbal Plants on the Growth Inhibition of Valsa ceratosperma Strains

정재훈,양덕춘 경희대학교 유전공학연구소 1990 遺傳工學論文集 Vol.2 No.-

These studies were conducted to control of apple cankers caused by Valsa ceratosperma strains by treatment of chemical compounds, antibiotics, extracts of herbal plants and compounds produced from mutant of Streptomyces melanosporofaciens. Effects of chemical control for apple canker were evaluated by growth inhibition of Valsa ceratosperma on the PDA media and the most effective chemical was found to be berberine followed by neoasogin, branchi Q, in general. Inhibitory concentration of Valsa ceratosperma was 500㎎/ℓ in berberine, and 10,000㎎/ℓin neoasogin. The antifungal antibiotic, cycloheximide, was effective for growth inhibition of Valsa ceratosperma; all strains were inhibited on the media with 100㎍/㎖ of cycloheximide and M26 strain was inhibited by lower concentration of 10㎍/㎖. Effects of extract of herbal plants for growth inhibition of Valsa ceratosperma were excellent with Phellodendron amurense and Coptis japonica, and especially in Coptisjaponica, low concentration of 1,000㎎/ℓ was effective Compounds produced from mutant of S melanosporofaciens for growth inhibition of Valsa ceratosperma were more effective than other compounds, i.e., chemical compounds, antibiotics and extracts of herbal plants. Minimum inhibitory concentration of compound C-1 for IF03025 and M1099 was 3125㎍/㎖.

접사함요에서 기원한 후비공 용종 1례

김중강,신무진,류태선,오춘석,김덕준 啓明大學校 醫科大學 1994 계명의대학술지 Vol.13 No.4

Choanal polyps can be defined as paranasal sinus polyps that pass through sinus ostia and protrude into the boundary between the nasal cavity and nasopharynx-the choana. The most common choanal polyp is the antrochoanal polyp, but sphenochoanal and ethmoidochoanal polyps are rarer. We experienced a case of choanal polyp originating from the nasal mucosa of the sphenoethmoid recess, not from the paranasal sinuses. It was removed with intranasal endoscopy, and 6 months follow-up has not shown any recurrence. So we report this very rare case with review of literatures.

인삼 Peroxidase(PgPrx3) 유전자의 분리 및 연초의 형질전환

손화,심주선,김세영,노영덕,김무성,양덕춘 경희대학교식량자원개발연구소 2005 硏究論文集 Vol.24 No.-

Peroxidase는 항산화에 관련된 효소이며 식물의 환경스트레스와 성장에 관련되는 중요한 유전자이다. 본 실험에서 사용된 Peroxidase Ⅲ의 cDNA는 Panax ginseng C.A. Meyer 잎에서 추출하였으며 PgPrx3라 명명하였다. 이 gene의 ORF영역은 1,065 bP이고 355개의 아미노산을 가지고 있다. BioEdit 프로그램을 이용하여 PgPrx3와 다른 식물의 peroxidase gene과 비교한 결과 상동성을 가지고 있었으며, Spinacia oleracea(70%), Vigna angulais(71%), Nicotiana tabacum(69%) and Linum usitatissimum(65%). 그중 Vigna angularis의 상동성이 가장 높았다. 담배에 본 유전자를 형질전환시켜 유전자의 특성을 검정하기 위하여 35S/35S/AMV/peroxidas/Tnos를 벡터 pRD400에 재조합 후 아그로박테리아를 이용하여 담배에 도입시켰다. 그리고 PCR를 이용하여 유전자 PgPrx3가 담배에 도입된 것을 확인하였으며, RT-PCR로 정상적으로 PgPrx3 유전자가 전사되어 RNA를 생성하고 있음을 확인하였다. A peroxidase (E.C. 1.11.1.7) is very important enzyme as antioxidants. The function of this gene is connected with growth and environmental stress of plant. A class Ⅲ peroxidase cDNA was isolated from the leaf of Panax ginseng C.A. Meyer and named as PgPrx3 which is consisted of an ORF (open reading frame) of 1,065 bp and an amino acid of 355 residues. BioEdit software was used to compare the PgPrx3 amino acid sequence with other plants that showed homologies with Spinacia oleracea (70%), Vigna angularis (71%), Nicotiana tabacum (69%) and Linum usitatissimum (65%). The peroxidase of Vigna angulatis was the most homologous with ginseng. The chimeric PgPrx3 gene, 35S/35S/AMV/peroxidas/ Tnos, was constructed in the binary vector pRD400. Agrobacterium tumefaciens strain LBA4404 harboring disarmed Ti-plasmid was used to transform Nicotiana tabacum L. using the leaf disc. Incorporation of the chimeric gene PgPrx3 into plants were confirmed by PCR analysis of genomic DNA and RT-PCR analysis of mRNA from transgenic tobacco. The PgPrx3 gene from ginseng was stably expressed in transgenic tobacco plants.