Substrate specificity of a recombinant d-lyxose isomerase from Providencia stuartii for monosaccharides

Kwon, H.J.,Yeom, S.J.,Park, C.S.,Oh, D.K. Society for Bioscience and Bioengineering, Japan ; 2010 Journal of bioscience and bioengineering Vol.110 No.1

The specific activity and catalytic efficiency (k<SUB>cat</SUB>/K<SUB>m</SUB>) of the recombinant putative protein from Providencia stuartii was the highest for d-lyxose among the aldose substrates, indicating that it is a d-lyxose isomerase. Gel filtration analysis suggested that the native enzyme is a dimer with a molecular mass of 44 kDa. The maximal activity for d-lyxose isomerization was observed at pH 7.5 and 45 <SUP>o</SUP>C in the presence of 1 mM Mn<SUP>2+</SUP>. The enzyme exhibited high isomerization activity for aldose substrates with the C2 and C3 hydroxyl groups in the left-hand configuration, such as d-lyxose, d-mannose, l-ribose, d-talose, and l-allose (listed in decreasing order of activity). The enzyme exhibited the highest activity for d-xylulose among all pentoses and hexoses. Thus, d-lyxose was produced at 288 g/l from 500 g/l d-xylulose by d-lyxose isomerase at pH 7.5 and 45 <SUP>o</SUP>C for 2 h, with a conversion yield of 58 % and a volumetric productivity of 144 g l<SUP>-1</SUP> h<SUP>-1</SUP>. The observed k<SUB>cat</SUB>/K<SUB>m</SUB> (920 mM<SUP>-1</SUP> s<SUP>-1</SUP>) of P. stuartiid-lyxose isomerase for d-xylulose is higher than any of the k<SUB>cat</SUB>/K<SUB>m</SUB> values previously reported for sugar and sugar phosphate isomerases with monosaccharide substrates. These results suggest that the enzyme will be useful as an industrial producer of d-lyxose.

Integral domains with finitely many spectral semistar operations

Chang, G. W.,Oh, D. Y. John Wiley Sons, Ltd 2017 Frontiers of mathematics in China Vol.12 No.1

<P>Let D be a finite-dimensional integral domain, Spec(D) be the set of prime ideals of D, and SpSS(D) be the set of spectral semistar operations on D. Mimouni gave a complete description for the prime ideal structure of D with SpSS(D) = n + dim(D) for 1 aecurrency sign n aecurrency sign 5 except for the quasi-local cases of n = 4, 5. In this paper, we show that there is an integral domain D such that SpSS(D) = n+dim(D) for all positive integers n with n not equal 2. As corollaries, we completely characterize the quasi-local domains D with SpSS(D) = n+dim(D) for n = 4, 5. Furthermore, we also present the lower and upper bounds of SpSS(D) when Spec(D) is a finite tree.</P>

Dosimetric comparison of axilla and groin radiotherapy techniques for high-risk and locally advanced skin cance

Malcolm D. Mattes,Ying Zhou,Sean L. Berry,Christopher A. Barker 대한방사선종양학회 2016 Radiation Oncology Journal Vol.34 No.2

Purpose: Radiation therapy targeting axilla and groin lymph nodes improves regional disease control in locally advanced and high-risk skin cancers. However, trials generally used conventional two-dimensional radiotherapy (2D-RT), contributing towards elatively high rates of side effects from treatment. The goal of this study is to determine if three-dimensional conformal radiation therapy (3D-CRT), intensity-modulated radiation therapy (IMRT), or volumetric-modulated arc therapy (VMAT) may improve radiation delivery to the target while avoiding organs at risk in the clinical context of skin cancer regional nodal irradiation. Materials and Methods: Twenty patients with locally advanced/high-risk skin cancers underwent computed tomography simulation. The relevant axilla or groin planning target volumes and organs at risk were delineated using standard definitions. Paired t-tests were used to compare the mean values of several dose-volumetric parameters for each of the 4 techniques. Results: In the axilla, the largest improvement for 3D-CRT compared to 2D-RT was for homogeneity index (13.9 vs. 54.3), at the expense of higher lung V20 (28.0% vs. 12.6%). In the groin, the largest improvements for 3D-CRT compared to 2D-RT were for anorectum Dmax (13.6 vs. 38.9 Gy), bowel D200cc (7.3 vs. 23.1 Gy), femur D50 (34.6 vs. 57.2 Gy), and genitalia Dmax (37.6 vs. 51.1 Gy). IMRT had further improvements compared to 3D-CRT for humerus Dmean (16.9 vs. 22.4 Gy), brachial plexus D5 (57.4 vs. 61.3 Gy), bladder D5 (26.8 vs. 36.5 Gy), and femur D50 (18.7 vs. 34.6 Gy). Fewer differences were observed between IMRT and VMAT. Conclusion: Compared to 2D-RT and 3D-CRT, IMRT and VMAT had dosimetric advantages in the treatment of nodal regions of skin cancer patients.

AGREEABLE DOMAINS

Anderson, D. D.,Kwak, Dong Je,Zafrullah, Muhammad 경북대학교 위상수학 기하학연구센터 1995 硏究論文集 Vol.4 No.-

An integral domain D with quotient field K is defined to be agreeable if for each fractional ideal F of D[X] with F ⊆ K[X] there exists 0 ≠ s ∈ D with sF ⊆ D[X]. D is agreeable ⇔ D satisfies property (*) (for 0 ≠ f(X) ∈ K[X], there exists 0 ≠ s ∈ D so that f(X)g(X) ∈ D[X] for g(X) ∈ K(X) implies that sg(X) ∈ D[X]) ⇔ D[X] is an almost principal domain, i.e., for each nonzero ideal I of D[X] with IK[X] ≠ K[X], there exists f(X) ∈ I and 0 ≠ s ∈ D with sI ⊆ (f(X)). If D is Noetherian or integrally closed, then D is agreeable. A number of other characterizations of agreeable domains are given as are a number of stability properties. For example, if D is agreeable, so is ??Dp_(a) and for a pair of domains D ⊆ D′ with [D: D′] ≠ 0, D is agreeable ⇔ D′ is agreeable. Results on agreeable domains are used to give an alternative treatment of Querre’s characterization of divisorial ideals in integrally closed polynomial rings. Finally, the various characterizations of D being agreeable are considered for polynomial rings in several variables.

비타민 D3 의 경구투여 또는 자외선 조사가 브로일러 병아리의 증체 및 비타민 D3 대사에 미치는 영향

장윤환(Y . H . Chiang),황선일(S . I . Hwang),김중달(J . D . Kim),M . F . Holick(M . F . Holick) 한국영양사료학회 1995 韓國營養飼料學會誌 Vol.19 No.6

When D((X)) and D{{X}} are Prufer domains

Chang, G.W.,Oh, D.Y. North-Holland Pub. Co 2012 Journal of pure and applied algebra Vol.216 No.2

Let D be an integral domain, X be an indeterminate over D, and D[[X]] be the power series ring over D. For f@?D[[X]], let c<SUB>D</SUB>(f) denote the ideal of D generated by the coefficients of f. Let N={f@?D[[X]]|c<SUB>D</SUB>(f)=D}, N<SUB>t</SUB>={f@?D[[X]]|c<SUB>D</SUB>(f)<SUB>t</SUB>=D}, D((X))=D[[X]]<SUB>N</SUB>, and D{{X}}=D[[X]]<SUB>N't</SUB>. We show that D is a Krull domain if and only if D{{X}} is a Prufer domain, if and only if D[[X]]<SUB>P[[X]]</SUB> is a valuation domain for each maximal t-ideal P of D, if and only if D[[X]] is a PvMD in which each t-ideal is divisorial. We also show that D is a Dedekind domain if and only if D((X)) is a Prufer domain, if and only if D[[X]]<SUB>M[[X]]</SUB> is a valuation domain for each maximal ideal M of D.

RF 스위치 적용을 위한 박막 PZT 엑추에이터의 d<SUB>31</SUB> 구동과 d<SUB>33</SUB> 구동 특성 비교

신민재(M. J. Shin),서영호(Y. H. Seo),최두선(D-S. Choi),황경현(K-H. Hwang) 한국정밀공학회 2006 한국정밀공학회 학술발표대회 논문집 Vol.2006 No.5월

In this work, we present the comparison between d<SUB>31</SUB> and d<SUB>33</SUB> mode characterization using the PZT micro-actuator for large displacement. The PZT micro-actuator consisted of Si, PZT, and Pt layer on SOI wafer. The electrode shapes were laminated and interdigitated for d<SUB>31</SUB> and d<SUB>33</SUB> mode, respectively. In order to characterize the actuation mode, we measured the displacement using laser interferometer. The maximum displacement of d<SUB>31</SUB> mode was 12.2㎛ at 10V, the actuation characterization of d<SUB>31</SUB> was better than that of d<SUB>33</SUB> mode. We estimated that displacement of d<SUB>33</SUB> mode would be larger than that of d<SUB>31</SUB> above 30V.

Measurement of D^*+/- meson production in e⁺/-p scattering at low Q²

ZEUS Collaboration,Chekanov, S.,Derrick, M.,Magill, S.,Miglioranzi, S.,Musgrave, B.,Nicholass, D.,Repond, J.,Yoshida, R.,Mattingly, M.C.K.,Jechow, M.,Pavel, N.,Yagues Molina, A.G.,Antonelli, S.,Antoni North-Holland Pub. Co 2007 Physics letters: B Vol.649 No.2

The production of D<SUP>*+</SUP>/-(2010) mesons in e<SUP>+</SUP>/-p scattering in the range of exchanged photon virtuality 0.05<Q<SUP>2</SUP><0.7 GeV<SUP>2</SUP> has been measured with the ZEUS detector at HERA using an integrated luminosity of 82 pb<SUP>-1</SUP>. The decay channels D<SUP>*+</SUP>->D<SUP>0</SUP>π<SUP>+</SUP> with D<SUP>0</SUP>->K<SUP>-</SUP>π<SUP>+</SUP> and corresponding antiparticle decay were used to identify D<SUP>*</SUP> mesons and the ZEUS beampipe calorimeter was used to identify the scattered electron. Differential D<SUP>*</SUP> cross sections as functions of Q<SUP>2</SUP>, inelasticity, y, transverse momentum of the D<SUP>*</SUP> meson, p<SUB>T</SUB>(D<SUP>*</SUP>), and pseudorapidity of the D<SUP>*</SUP> meson, η(D<SUP>*</SUP>), have been measured in the kinematic region 0.02<y<0.85, 1.5<p<SUB>T</SUB>(D<SUP>*</SUP>)<9.0 GeV and |η(D<SUP>*</SUP>)|<1.5. The measured differential cross sections are in agreement with two different NLO QCD calculations. The cross sections are also compared to previous ZEUS measurements in the photoproduction and DIS regimes.

Administration of vitamin D3 by injection or drinking water alters serum 25-hydroxycholecalciferol concentrations of nursery pigs

Young Dal Jang,Jingyun Ma,Ning Lu,Jina Lim,H. James Monegue,Robert L. Stuart,Merlin D. Lindemann 아세아·태평양축산학회 2018 Animal Bioscience Vol.31 No.2

Objective: Two experiments were conducted to evaluate vitamin D3 administration to nursery pigs by injection or in drinking water on serum 25-hydroxycholecalciferol (25-OHD3) concentrations. Methods: At weaning, 51 pigs (27 and 24 pigs in experiments 1 and 2, respectively) were allotted to vitamin D3 treatments. Treatments in experiment 1 were: i) control (CON), no vitamin administration beyond that in the diet, ii) intramuscular (IM) injection of 40,000 IU of vitamin D3 at weaning, and iii) water administration, 5,493 IU of vitamin D3/L drinking water for 14 d postweaning. Treatments in experiment 2 were: i) control (CON), no vitamin administration, and ii) water administration, 92 IU of d-α-tocopherol and 5,493 IU of vitamin D3/L drinking water for 28 d postweaning. The lightest 2 pigs within each pen were IM injected with an additional 1,000 IU of d-α-tocopherol, 100,000 IU of retinyl palmitate, and 100,000 IU of vitamin D3. Results: In both experiments, serum 25-OHD3 was changed after vitamin D3 administration (p<0.05). In experiment 1, injection and water groups had greater values than CON group through d 35 and 21 post-administration, respectively (p<0.05). In experiment 2, serum values peaked at d 3 post-administration in the injection groups regardless of water treatments (p<0.05) whereas CON and water-only groups had peaks at d 14 and 28 post-administration, respectively (p<0.05). Even though the injection groups had greater serum 25-OHD3 concentrations than the non-injection groups through d 7 post-administration regardless of water treatments (p<0.05), the water-only group had greater values than the injection-only group from d 21 post-administration onward (p<0.05). Conclusion: Serum 25-OHD3 concentrations in pigs increased either by vitamin D3 injection or drinking water administration. Although a single vitamin D3 injection enhanced serum 25-OHD3 concentrations greater than water administration in the initial period post-administration, a continuous supply of vitamin D3 via drinking water could maintain higher serum values than the single injection.

여러가지 종류의 사료 섬유질을 섭취하는 쥐의 수분 및 Na 대사에 관한 연구

이봉덕,권순기,이수기 ( B . D . Lee,S . K . Kwon,S . K . Lee ) 한국축산학회 1986 한국축산학회지 Vol.28 No.8

The effects of several sources of dietary fiber on the water and sodium (Na) metabolism of rats were investigated. Wheat bran (D2), pure cellulose (D3), and ground rice straw (D4) replaced corn in the control diet (D1) at the level of 10%: pectin (DS), which is water-soluble, replaced corn at 5% level. In the growth trial with 45 female weanling rats (Sprague Dawley strain), the growth rate of DS was significantly (P≤.05) lower than those of D1 and D2. No difference in growth rate was found among D3, D4 and D5. With regard to water intake of growing rats, there was no difference among all treatments. In metabolism trial with 30 adult male rats, the dry matter (DM) digestibilities of D3 and D4 were significantly lower than those of D1, D2 and D5. Similar DM digestibilities were found in D1, D2 and D5. As in the case of growing rats, no difference was found in water intake among five treatments. With regard to water holding capacity (WHC) of feces, D3 and D4 showed significantly larger values than D2. The WHC of D1 and D5 were even lower (P≤.05) than D2. The bulls density (BD) of feces was exactly in the inverse relationship with WHC. The BD of D3 and D4 were significantly smaller than the other treatments. D1 and D5 showed significantly larger BD than did D2. In terms of Na excretion routes of urine and feces, D1 and DS excreted significantly more Na via urine than did D3 and D4. The D2 showed intermediate values in this respect. No difference was found in Na^+ and osmotic concentrations either in plasma or urine among dietary treatments. With regard to plasma clearances, there was no difference among all treatments in C_(Na)^+, C_(osm) anti C_(H₂O), The C_(H₂O) values from all treatments showed negative values, indicating that the rats were removing excess solutes in body fluids via urine. Among the water-insoluble fibers, wheat bran appeared to be less fibrous than pure cellulose or ground rice straw in several respects, i.e., growth rate, feral WHC and BD, and the route of Na excretion. Except that it inhibits the growth rate of young rats, pectin brought about the same effect as did the control diet, indicating that the gut microflora fermented the water-soluble pectin. Different nutritional and physiological effects might be expected from rats fed dietary fibers having different solubility in water.