Paulo Freire의 생애와 작품세계

Denis Collins,양견 한국교육철학회 1991 교육철학 Vol.9 No.-

Diagnosis of Bovine Paratuberculosis by a Novel Enzyme-Linked Immunosorbent Assay Based on Early Secreted Antigens of Mycobacterium avium subsp. paratuberculosis

Shin, Sung Jae,Cho, Donghee,Collins, Michael T. American Society for Microbiology 2008 CLINICAL AND VACCINE IMMUNOLOGY Vol.15 No.8

<B>ABSTRACT</B><P>We previously reported that protein antigens of serodiagnostic potential were more abundant in culture filtrates than cellular extracts from liquid cultures of <I>Mycobacterium avium</I> subsp. <I>paratuberculosis</I> (D. Cho and M. T. Collins, Clin. Vaccine Immunol. 13:1155-1161, 2006). Based on this observation, a novel enzyme-linked immunosorbent assay (ELISA) using antigens secreted by young (early- to mid-log-phase) cultures of <I>M. avium</I> subsp. <I>paratuberculosis</I> JTC303 (a low-passage isolate originating from the ileum of a Holstein bull) in mycobactin-supplemented Watson-Reid medium (pH 6.0) was developed and evaluated using a previously described panel of bovine sera (M. T. Collins et al., Clin. Diagn. Lab. Immunol. 12:685-692, 2005) that included 444 paratuberculosis cases and 412 controls. The new assay, called JTC-ELISA, had a significantly higher diagnostic sensitivity and an equivalent specificity compared to those of five commercial paratuberculosis ELISA kits. By receiver-operating characteristic analysis, the JTC-ELISA had the highest area under the curve of the six assays evaluated. The JTC-ELISA was particularly sensitive at detecting low-level fecal shedders of <I>Mavium</I> subsp. <I>paratuberculosis</I> (40%; the sensitivity of the commercial kits was 20%). The JTC-ELISA works effectively on both serum and milk samples for the detection of cattle with subclinical <I>M. avium</I> subsp. <I>paratuberculosis</I> infections, providing a cost-effective diagnostic tool to support paratuberculosis control programs in cattle herds.</P>

Assessment of Equivalent Elastic Modulus of Perforated Spherical Plates

collins juma,남궁인 한국압력기기공학회 2019 한국압력기기공학회 논문집 Vol.15 No.1

Perforated plates are used for the steam generator tube-sheet and the Reactor Vessel Closure Head in the Nuclear Power Plant. The ASME code, Section III Appendix A-8000, addresses the analysis of perforated plates, however, this analysis is only limited to the flat plate with a triangular perforation pattern. Based on the concept of the effective elastic constants, simulation of flat and spherical perforated plates and their equivalent solid plates were carried out using Finite Element Analysis (FEA). The isotropic material properties of the perforated plate were replaced with anisotropic material properties of the equivalent solid plate and subjected to the same loading conditions. The generated curves of effective elastic constants vs ligament efficiency for the flat perforated plate were in agreement with the design curve provided by ASME code. With this result, a plate with spherical curvature having perforations can be conveniently analyzed with equivalent elastic modulus and equivalent Poisson’s ratio.

Equivalent Material Properties of Thin and Thick Perforated Plate by FEM simulation

Collins Owino JUMA,Ihn NAMGUNG 대한기계학회 2018 대한기계학회 춘추학술대회 Vol.2018 No.12

Perforated plates are used for the steam generator tube sheet and the Reactor Vessel Closure Head in the Nuclear Power Plant. The structural integrity of these perforated structures is vital to the operation and safety of the Nuclear Power plant. ASME code addresses the analysis of perforated plates in Section III, Appendix A-8000, with triangular perforation pattern. This paper investigates the equivalent material properties by FEM methodology. Simulation of both thin and thick perforated plates and try to re-assess ASME Section III Appendix A-8000 curve. The isotropic material properties of the perforated plate were replaced with anisotropic material properties of the equivalent solid plate and subjected to same loading and boundary conditions. The difference between the deformation of the perforated and equivalent solid plate was optimized for convergence by varying the effective elastic constants. Curves of the effective elastic constants vs ligament efficiency were generated. The equivalent material property curves obtained using FEM methodology were in good agreement with the design curve provided in ASME Section. III Appendix A-8000.

Combined cerebral and somatic near-infrared spectroscopy oximetry monitoring during liver surgery: an observational and non-interventional study

Collin Yves,Hu Tina,Denault André,Fortier Annik,Beaubien-Souligny William,Lapointe Réal,Vandenbroucke-Menu Franck 대한마취통증의학회 2022 Korean Journal of Anesthesiology Vol.75 No.5

Background: Cerebral oximetry using near-infrared spectroscopy (NIRS) is used for monitoring cerebral oxygen saturation during cardiac surgery and is correlated with clinical outcomes. Our goal was to explore cerebral and somatic NIRS in liver resections as a predictor of post-operative complications. Methods: Prospective observational and non-interventional study from a tertiary care university hospital including adult patients undergoing liver resection monitored using NIRS at four sites before and during surgery. Those sites were: frontotemporal left and right zones, right thigh, and right arm. Anesthesiologists and surgeons were blinded to oximetry values. Correlations were assessed between baseline oximetry values and cerebro-somatic desaturation load (threshold of 80% from baseline) values with peri-operative events and complications. Results: Ninety patients were distributed equally among gender with a mean age of 59.7 ± 13.1 years. Lower baseline cerebral and/or somatic values were associated with increased risk of delirium, respiratory failure, surgical and renal complications, blood transfusions, and length of stay in the intensive care unit and in the hospital (P < 0.05). The severity of somatic desaturation below 80% was the only parameter associated with blood losses (P = 0.030) and length of hospital stay (P = 0.047). Conclusions: Cerebral and somatic desaturation does occur in liver resection and can be used simultaneously during liver surgery. Both baseline cerebral and somatic NIRS values are correlated with complications and outcomes. However, thigh desaturation appears more sensitive than cerebral NIRS values in predicting some of these complications.

Review : Agricultural Process and Food Engineering ; Current State of Postharvest Fruit and Vegetable Management in East Africa

( Collins Wakholi ),( Byoungkwan Cho ),( Changyeun Mo ),( Moon S. Kim ) 한국농업기계학회 2015 바이오시스템공학 Vol.40 No.3

Purpose: Fruit and vegetable production is a fast-growing sector in East Africa, and it bears considerable local and international market potential. In an effort to analyze the challenges within this sector and suggest possible solutions, this study reviews the postharvest handling technologies commonly used with fruits and vegetables in East Africa. Methods: During the course of this study, small-scale farmers were identified as the most prominent producers of fruit and vegetable crops in the region. Results: We found that many of these small-scale farmers employed relative simple and inexpensive techniques in handling their limited volumes of produce. Several factors could be addressed to reduce postharvest losses, including weak policies, inferior infrastructure, and poor market strategies. However, the lack of basic knowledge (including demographic, scientific, and economic knowledge) among the stakeholders (e.g., researchers, farmers, governments, nongovernment organizations, and merchants) on how to develop, implement, use, and sustain the recommended handling technologies is probably the most problematic. Conclusions: We recommend that high priority be placed on closing the knowledge gap, which could enhance the efforts of all the stakeholders to address and reduce postharvest losses.

The Effect of Light on Champagne Yeast Cell Growth and Ethanol Production Under Variable pH Conditions

Collins, Paul C.,Schnelle, Karl B.,Malaney, Jr.George W.,Tanner, Robert D. The Korean Society for Biotechnology and Bioengine 1991 KSBB Journal Vol.6 No.2

The effect of wtlitc light on unaeraten growth of Baker's yeast and the accompanying ethanol production has been studied in a batch process at 27$^{\circ}C$. Over the 80-hour period of the Champagne yeast process without pH control, the cull growth was inhibited by the fluorescent light. Another observed difference between the runs is that the drop and subsequent rise in redox potential occurred much sooner in the fermentation with light than in the fermentation without light. This preliminary study indicated that ethanol production could be enhanced by light as the cell concentration is repressed. The possible pathway, shift of the sugar substrate toward ethanol and away from cells was manifested by another difference as well. As observed under the microscope, many of the yeast cells grown under light budded without dividing by the normal fission process as they did in the dark. Furthermore, the undivided and branched (light grown) cell did not agglutinate at the end of the fermentation process as did the distinct spherical (dark grown) cells.

Development of an Online measurement of corn seed viability using hyperspectral imaging

( Collins Wakholi ),( Youngwook Seo ),( Byoung-kwan Cho ) 한국농업기계학회 2016 한국농업기계학회 학술발표논문집 Vol.21 No.2

The ability to classify seeds according to their respective traits like viability, variety etc. is key to regulation and ensuring seed quality and purity. Since the mass inception of genetically modified (GM) seeds to the market, many undesirable effects have been observed and are able to pose danger to the natural ecosystem and to humans at large. On top of the undesirable effects, there is a need to keep the natural gene pool as pure as possible from mixing with GM genes thus calling for careful caution during the handling of GM organisms. Currently many regulations have been imposed on the trade and import of GM seeds. In some societies for instance, the imported GM seeds have to be either killed or traded under very careful certification conditions. Clearly, there is a need to develop cutting edge technology to detect Viable GM seeds before import or trade. The main purpose of this research was to develop a working online sorting system based on Short Wave Infrared (SWIR) hyperspectral imaging (HSI) that is able to detect and sort GM corn seeds according their viability. In this study, hyperspectral image data from 600 corn seeds (50% microwave treated, 50% as control) was collected, analyzed and used to build classification models to classify between treated (non-viable) and non-treated (control/viable) corn groups. Three classification models were tested which included Partial Least Squares-Discriminant Analysis (PLS-DA), Support Vector Machines (SVM) and Linear Discriminant Analysis (LDA). The PLS-DA model coupled with some image processing tools (2-D median filter and morphological operations) performed the best and was applied to the online sorting system and tested its performance at different conveyor belt speeds until optimum. The overall system classification accuracy from the online tests was found to be 84% and 81% for white and purple corn respectively at conveyor belt speed of about 72 mm/s. The overall system accuracy was found to be lower than that produced from the offline spectral analysis possibly due to effects like ambient temperature, electronic noise, test sample dissimilarity, etc. The results show that the hyperspectral based online sorting prototype is able to be used for non-destructive real-time inspection of corn seed viability which could be faster and cheaper alternative to the conventional measurement methods which are expensive, time consuming and representative.

Application of modified red mud in environmentally-benign applications: A review paper

Collin G. Joseph,Yun Hin Taufiq-Yap,Vigneswar Krishnan,Gianluca Li Puma 대한환경공학회 2020 Environmental Engineering Research Vol.25 No.6

Red mud (RM) is a waste product that results from bauxite refining via the Bayer process. Its disposal remains an issue which raises significant environmental concerns, particularly if disposed on land or water bodies. Much research has been done on the use of red mud for environmentally-benign applications such as wastewater treatment, catalysis, the production of construction materials and glass ceramics, and for the recovery of metals. This paper reviews the current efforts made in the utilization of red mud as a valuable industrial by-product, which in turn should minimize its harmful impact on the environment. This detailed review compiles and highlights a variety of novel applications of modified red mud as a coagulant, an adsorbent for wastewater treatment, as well as, its use in catalytic processes and in building materials. The physico-chemical properties of red mud can be tuned by a range of treatment methods include acidification, neutralization and heat treatment. As revealed from the literature reviewed, modifications on red mud for the removal of various types of contaminants have shown promising results. However, further amendment and modifications on red mud are needed to utilize this industrial waste in many other industrial applications.

Comparison between Tetrazolium and Germination Test Results for Melon Seed Viability Determination using Hyperspectral Imaging

( Collins Wakholi ),( Byoung-kwan Cho ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1

Tetrazolium (TZ) test is one of the most reliable and widely used method in research to determine seed viability. The TZ test is able approximate seed viability through determining whether a seed’s tissue is still biochemically active or not. Despite the accurate prediction of viability by germination tests, the TZ test is preferred because it is relatively quick compared to germination tests that take anywhere between days to weeks to acquire results. The objective of this study was to compare the TZ test with germination tests (proved to give a realistic picture of seed viability) results using Short wave infrared, SWIR Hyperspectral imaging, HIS data and multivariate data analysis. For this study, damage-free melon (cucumis melo) seeds were used and were randomly divided into two groups of 1000 seeds each. Hyperspectral imaging data of both groups were collected using the same settings and under same environment. One group was subjected to germination tests while the other to TZ test to determine the viability of the seeds. The performance of the multivariate classification models achieved based on germination tests and TZ test results were compared. A clear difference in the classification model performances for germination and TZ test based models were observed. The results of this study serve as a key foundation in the development of a real-time sorting system based on HSI for melon seeds.