Blocking of Cell Proliferation, Cytokines Production and Genes Expression Following Administration of Cordyceps Sinensis in the Bronchoalveolar Lavage Fluids Cells

Kuo, Yuh-Chi,Wu, Chia-Lin,Tasi, Wei-Jern,Wang, Jir-Yenn,Chang, Shi-Chung,Lin, Ching-Yuang,Shiao, Ming-Shi 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Yuh-Chi Kuo¹,Chia-Lin Wn¹,Wei-jern Tasi¹,Jir-Yenn Wang²,Shi-Chung Chang₃,Ching-Yung Lin⁴,and Ming-Shi Shiao⁴¹National Research Institute of Chinese Medicine, ²Department of Biology,Fu Jen University, ³Department of Chest, ⁴Department of Pediatrics, □De[art,amt of Medical Research and Education. Taipei Veterans General Hospital, Taipei, Taiwan Yuh-Chi Kuo¹,Chia-Lin Wn¹,Wei-jern Tasi¹,Jir-Yenn Wang²,Shi-Chung Chang²,Ching-Yung Lin³,and Ming-Shi Shiao⁴.¹National Research Institute of Chinese Medicine, ²Department of Biology,Fu Jen University, ³Department of Chest, ⁴Department of Pediatrics, □De[art,amt of Medical Research and Education. Taipei Veterans General Hospital, Taipei, Taiwan. Blocking of Cell Proliferation, Cytokines Production and Genes Expression Following Administration of Cordyceps sinensis in the Bronchoalveolar Lavage Fluids Cells. Proceedings of international Symposium on East-West Medicine, Seoul. 44-64, 1999.-Cordyceps sinensis is a major parasitic fungus on larva of Lepidoptera. It is one of the well know fungi used in traditional Chinese medicine for treatment asthma, lung and kideney diseases. Many fungi belonging to the genus Cordyceps have been demonstrated to produce natural products with various biological activities. Reported biological activities of genus cordyceps include:(a)inhibition of DNA and RNA synthesis;(b)enhancement of cell differentiation;(c)restruction of cytoskeleton;(d)inhibition of protein kinase activity;(e)antitumor activity on bladder, colon, lung carcinoma as well as fibroblastoma;(f)inhibition of the infection and revers transciptase activity of human immunodeficiency virus type 1; and(g) inhibition of methylation of nucleic acid. Thus, it is suggested that the extracts of Cordyceps sp. Contain antitumor, antiviral, and many other biological activities. In our previous studies, we proved that C. sinensis contains the antitumor and immunomodulatory agents. In the present studies, effects of C. sinensis on bronchoalveolar lavage fluids(BAL)cells were demonstrated. The crude methanolic extracts of C. sinensis were fractionated into 15 fractions by silica gel column chromatography. Effects of various fraction on BAL cells proliferation and lipopolysaccharide(LPS) stimulated interleukin-1β(IL-8),platelet activating factor(PAF), interleukin-6(IL-6), tumor necrosis factor-α(TNF-α),and interleukin-8(IL-8) production on BAL cells were determined. The result indicated that the CS-19-22 fraction suppressed BAL cells proliferation activated by LPS. The median inhibitory concentration(IC50) was 6㎍/ml. The CS-19-22 fraction also decreased IL-1β,PAF,IL-6, TNF-αand IL-8 production.The results of revers transcription-polymerase chain reaction(RT-PCR) demonstrated that the CS-19-22 fraction did not affect IL-1β,PAF,IL-6, TNF-α,and IL-8 mRNAs expression in BAL cells activated by LPS. By contrast, it inhibited the IL-10 mRNA expression but enhanced IFN-γand IL-12 mRNAs expression in activated BAL cells. Moreover, the CS-19-22 fraction blocked PAF-induced platelet aggregation. It is unlikely that cytotoxicity was involved. Because no cell deths were observable. We hypothesize that inhibitory mechanisms of CS-19-22 on BAL cells proliferation may be related to the impairments of gene expression and production of cytokines in BAL cells. The explanation of C. sinensis antiasthma function may involved the following(1)C.sinenesis contained immunomodulatory agents that stimulated IFN-γand IL-12 expression in TH1cells.(2)Enhanced secretion of IFN-γand IL-12 will inhibit the Tγcells immune responses Ex. Decreasing of IL-6 and IL-10 production.(3) The impairments of cytokines production in TH2 cells suppressed th BAL cells proliferation then more cytokines production Ex.IL-1β,PAF,IL-6,TNF-αand IL-8 in activated BAL cells were decreased. (4)The suppression of TH2cells immune responses will inhibit the growth and differentiation of B cells then IgE production will be decreased. (5)Reduced production of IgE will decreased asthma attack occurring in individuals. However, the results of the present study suggested that C. sinensis may also have acted to treat asthma in part by inhibiting BAL cells proliferation and cytokine gene expression and production. Future, experiments with treatment of animals with asthma with C. sinensis will be necessary to defined whether C. sinensis can reduce asthma injury. Plans are underway for the isolation of pure principal growth inhibitors and their mechanisms of action are subjected for further study.

설계자동화를 위한 VHDL Analyzer에 관한 연구

印致虎 世明大學校 1992 世明論叢 Vol.2 No.-

This paper has developed the automation system for logic design, using widely available VHDL language as the standard hardware description language[1][8][9], which extracts the efficient FSM(Finite State Machine) List in automatic optimized synthesis for the practical ASIC hardware from the design technique of the register transfer level. VHDL analysis process in this paper is similar to compiler process in software area, is partitioned into the VHDL parsing, the Behavioral transformation, the Basic block recognition, and the Dependency analysis, etc[5][7]. VHDL parsing resulted in the FSM (Finite State Machine) List efficient in Data Path and Control Path synthesis for logic design with creating the intermediate code in AST(Abstract Syntax Tree) for the intermediate symbol table.

ASIC설계 자동화를 위한 새로운 최소자원 스케쥴링 알고리듬

인치호 世明大學校 1998 世明論叢 Vol.7 No.-

This thesis presents a new VHDL intermediate format CDFG(Control Data Flow Graph) an a new scheduling algorithm for an optimal control dominated ASIC design. The proposed CDFG is a control flow graph whcih represents conditional brances and loops efficiently. Also it represents ata dependency and such constraints as hardware resource and timing. In scheduling algorithm, the contraints are substituted by subgraphs, and then the number of subgraphs (that is the number of the constraints) is minimized by using the inclusion and overlap relation among subgraphs. The effectiveness of the proposed algorithm has been proven by the experiment with the bnchmark examples.

움직임 추정을 위한 시스톨릭 어레이를 사용한 효율적인 VLSI 구조

진용선,인치호 世明大學校 1997 世明論叢 Vol.6 No.-

In this paper. an efficient VLSI architecture for Full search Block Matchin Algorithm (FBMA) with systolic arrays is proposed. Because FBMA for motion estimation is simple, regular. and modular. its suitable for VlSI implementation with systolic arrays. Considering the data flow on overlapping search areas, which exist in both the same and perpendicular direction to searching course, the redundancy of duplicated data input for each reference block is removed to enhance the performance of FBMA.

설계 자동화를 위한 하드웨어 할당 알고리듬

최지영,인치호 世明大學校 1998 世明論叢 Vol.7 No.-

This thesis proposes a new hardware allocation algorithm for design automation. The proposed algorithm works on scheduled input graph and simultaneously allocates binds functional units, interconnections and registers by considering interdependency between operations and storage elements in each control step, in order to share registers and interconnections connected to functional units, as much as possible. This thesis shows the effectiveness of the algorithm by comparing the results of our experiments with those of exiting system.

A Review of Finite Element Modeling for Anterior Cervical Discectomy and Fusion

Lin Maohua,Paul Rudy,Dhar Utpal Kanti,Doulgeris James,O’Connor Timothy E.,Tsai Chi-Tay,Vrionis Frank D. 대한척추외과학회 2023 Asian Spine Journal Vol.17 No.5

The cervical spine poses many complex challenges that require complex solutions. Anterior cervical discectomy and fusion (ACDF) has been one such technique often employed to address such issues. In order to address the problems with ACDF and assess the modifications that have been made to the technique over time, finite element analyses (FEA) have proven to be an effective tool. The variations of cervical spine FEA models that have been produced over the past couple of decades, particularly more recent representations of more complex geometries, have not yet been identified and characterized in any literature. Our objective was to present material property models and cervical spine models for various simulation purposes. The outlining and refinement of the FEA process will yield more reliable outcomes and provide a stable basis for the modeling protocols of the cervical spine.

Phase I and pharmacokinetic study of cisplatin and troxacitabine administered intravenously every 28 days in patients with advanced solid malignancies

Lin, Chia-Chi,Beeram, Muralidhar,Rowinsky, Eric K.,Takimoto, Chris H.,Ng, Chee M.,Geyer Jr, Charles E.,Denis, Louis J.,De Bono, Johann S.,Hao, Desiree,Tolcher, Anthony W.,Rha, Sun-Young,Jolivet, Jacqu Springer-Verlag 2009 Cancer chemotherapy and pharmacology Vol.65 No.1

Maternal interpretation of caring for children with Biliary Atresia in Taiwan

Chi-Hua Lin,Tzu-Ying Lee,Chuen-Bin Jiang 한국간호과학회 2023 한국간호과학회 학술대회 Vol.2023 No.11

A New Scheduling Algorithm to Enable Power Management for Low Power

Chi-Ho Lin,Gi-bok Kim,Hi-seok Kim 대한전자공학회 2003 ITC-CSCC :International Technical Conference on Ci Vol.2003 No.7

Extracting parameters of TMD and primary structure from the combined system responses

Chi-Chang Lin,Jer-Fu Wang 국제구조공학회 2015 Smart Structures and Systems, An International Jou Vol.16 No.5

Tuned mass dampers (TMDs) have been a prevalent vibration control device for suppressing excessive vibration because of environmental loadings in contemporary tall buildings since the mid-1970s. A TMD must be tuned to the natural frequency of the primary structure to be effective. In practice, a TMD may be assembled in situ, simultaneously with the building construction. In such a situation, the respective dynamic properties of the TMD device and building cannot be identified to determine the tuning status of the TMD. For this purpose, a methodology was developed to obtain the parameters of the TMD and primary building on the basis of the eigenparameters of any two complex modes of the combined building–TMD system. The theory was derived in state-space to characterize the nonclassical damping feature of the system, and combined with a system identification technique to obtain the system eigenparameters using the acceleration measurements. The proposed procedure was first demonstrated using a numerical verification and then applied to real, experimental data of a large-scale building–TMD system. The results showed that the procedure is capable of identifying the respective parameters of the TMD and primary structure and is applicable in real implementations by using only the acceleration response measurements of the TMD and its located floor.