cDNA Cloning and Genomic Structure of Glyceraldehyde-3-Phosphate Dehydrogenase from the Entomopathogenic fungus, Paecilomyces tenuipes-Jocheon

Nam Sook Park,Han Seok Kang,Seon Ku Kim,Yong Gyun Kim,Byung Uuk Cho,Teak Soon Shin,Keun Ki Kim,Hyun Chul Park,Hong Joo Son,Hong Gu Lee,Sang Mong Lee 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05

Fungi belonging to the Paecilomyces spp. have recently been used as food and herbal medicines in Korea and are greatly popular as commercially available powdered supplement or dried fruiting body. Despite this acceptance and its use, little is known of the genes related to its reactive agents. Presently, We have constructed an olig-d(T) primed directional cDNA library from the silkworm Dongchunghacho, an entomopathogenic fungus, of which species is belonging to Paecilomyces spp. based on the previous identification of ITS1 and ITS2 at the molecular level and collected from Jocheon Miryang, Korea. To isolate and screen genes in the fungus, 626 expressed sequence tags(ESTs) were generated by a partial sequencing from the cDNA library. cDNA encoding the glyceraldehyde-3-phosphate dehydrogenase(Pt-GAPDH) of Paecilomyces tenuipes- Jocheon was cloned from the above cDNA library. The complete cDNA sequence of Pt-GAPDH is comprised of 1,014bp encoding 338 amino acid residues. The deduced protein sequence of Pt-GAPDH showed higher homology with Beauberia bassiana-GAPDH(93% amino acid identity). Hydropathy analysis revealed that Pt-GAPDH protein is hydrophilic. The major three amino acids in its composition of amino acid residues were alanine(11.54%), valine(9.47%) and glycine(8.88%). The Pt-GAPDH gene of Paecilomyces tenuipes entomopathogenic fungus consisted of three exons and two introns coding for 338 amino acid residues, and the genomic DNA length of the gene spans 1302bp. The accession number of the gene in GenBank are GU997099 for Pt-GAPDH cDNA and GU997102 for Pt-GAPDH genomic DNA. More investigation works including gene expression, immunological analysis etc. will be carried continuously without hesitation after this presentation.

Molecular Cloning, Expression and Genomic Structure of Glyceraldehyde-3-Phosphate Dehydrogenase from the Entomopathogenic fungus, Paecilomyces tenuipes Jocheon-1

Nam Sook Park,Han Seok Kang,Seon Ku Kim,Yong Gyun Kim,Byung Uuk Cho,Teak Soon Shin,Keun Ki Kim,Hyun Chul Park,Hong Joo Son,Hong Gu Lee,Sang Mong Lee 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05

Presently, We have constructed an olig-d(T) primed directional cDNA library from the silkworm Dongchunghacho, an entomopathogenic fungus, of which species is belonging to Paecilomyces tenuipes Jocheon-1. To isolate and screen genes in the fungus, 626 expressed sequence tags(ESTs) were generated by a partial sequencing from the cDNA library. Paecilomyces tenuipes Jocheon-1 cDNA encoding the glyceraldehyde-3-phosphate dehydrogenase(Pt-GAPDH) of Paecilomyces tenuipes Jocheon-1 was cloned from the above cDNA library. The complete cDNA sequence of Pt-GAPDHis comprised of 1,014bp encoding 338 amino acid residues. The deduced protein sequence of Pt-GAPDH showed higher homology with Beauberia bassiana-GAPDH(93% amino acid identity). Hydropathy analysis revealed that Pt-GAPDH protein is hydrophilic. The major three amino acids in its composition of amino acid residues were alanine(11.54%), valine(9.47%) and glycine(8.88%). The cDNA encoding Pt-GAPDH was expressed as a 37 kDa polypeptide in baculovirus-infected insect Sf9 cells. The Pt-GAPDH gene of Paecilomyces tenuipes entomopathogenic fungus consisted of three exons and two introns coding for 338 amino acid residues, and the genomic DNA length of the gene spans 1302bp. The accession number of the gene in GenBank are GU997099 for Pt-GAPDH cDNA and GU997102 for Pt-GAPDH genomic DNA.

여성의 피부관리실태와 관련요인

구재희,남철현,최연희,배향선,신옥순,양숙희,이명옥 慶山大學校 保健福祉硏究所 2002 保健福祉硏究 Vol.7 No.-

This study was conducted to analyze aspects of women's skin care and related factors in order to contribute to community residents's right skin care. The subjects of this study was 800 women who were 15 to 65 years old. Data were collected in Daegu City and Kyungsan City from June 21, 2002 to August 18, 2002. The results of this study can be summarized as follows. 1. According to socio-demographic characteristics of the respondents, 26.8% of them were in twenties. 50.9% of them was students at colleges or graduated from collges. 38.3% was students and 37.5% was housewives. 55.5% belonged to the middle economic class. 2. 47.9% of the respondents replied that they were in good health. 57.2% of them had fair skin, while 25.1% had dark skin. According to health condition of face skin, 61.5% was in regular condition, while 26.0% was in bad condition. 29.9% had drying face skin and 21.3% had neutral or complex character of face skin. 3. Among the reasons they had trouble with face skin, the proportion of having the rough skin was highest (25.0%). 16.6% replied that they had trouble with a lot of wrinkles and 16.3% replied that they had trouble with dark face skin. 25.4% responded that their face skin was too tight. 13.3% replied that they had trouble with scars. 4. The factors significantly influencing the experience of skin care were age, education level, residence, experience of side effect of cosmetics, experience of skin diseases on face or body, experience of trouble after receiving skin care, and experience of trouble with acne. From above results, it can be concluded that selection of proper skin care rooms is important in receiving desirable skin care and preventing side effect related to skin care. The women also must be cautious in selecting and using cosmetics and massage pack. The government, related organizations, and skin and beauty specialists must make efforts to develop education programs for healthy skin care.

스포츠 스쿠버 다이버의 심해 다이빙 기술 분석

이장구,구정철,신철호 한국학교체육학회 2002 한국학교 체육학회지 Vol.12 No.2

본 연구는 스포츠 스쿠버 다이버가 안전수칙을 준수하며 심해다이빙을 안전하게 하고 있는지 그 실태를 분석하는데 있다. 연구 목적을 달성하기 위하여, 다이버 9명의 다이빙 컴퓨터를 회수한 후, SUUNTO dive log 프로그램과 다이빙 기록 분석도구를 이용해서 자료를 수집하였다. 다이빙 사례 37회를 첫 잠수와 재 잠수로 비교 분석하였으며, 분석 결과 다음과 같은 결론을 얻었다. 첫째, 다이버의 다이빙 한계 수심 초과 빈도는 첫 잠수 시 5회(13.5%)로 나타났다. 둘째, 다이버는 분당 9m이하의 상승속도를 준수하였다. 셋째, 다이버의 안전 감압 미실시 빈도는 첫 잠수 시 16회(43%), 재 잠수 시 10회(27%)로 나타났다. 넷째, 다이버는 재 잠수 시 전체 다이빙 시간을 2.7분 더 소요하는 것으로 나타났다. 다섯째, 다이버는 무감압 한계 시간 내에서 안전하게 다이빙을 하고 있었다. The purpose of this study was to analysis the deep diving actual condition of safety rule of to observe sport SCUBA divers. An analysis first dive and repeated 37 cycle, use SUUNTO dive log program and dive log analysis tools, the right of restitution of diving computer of 9 diver's. The study has achived the following findings. First, the number of times of times of depth limit to exceed, showed 5(13.5%) at the first dive. Second, all divers observed the velocity of ascent per minute under 9m. Third, the divers' failure number of times of observing the safety decompression stop showed 16(43%) at the first dive and 10(27%) at the repeated dive. Fourth, divers' average total dive time was 27.6minutes at the first dive and 30.3 minutes at the repeated dive. Fifth, they dived safely within the no-decompression limit.

청자 경어법의 '-이-' 형태소 분석 재고찰

허철구 국립7개대학공동논문집간행위원회 2003 人文學論叢 Vol.3 No.-

Recently Korean grammarians have described the grammatical characteristics of final endings and prefinal endings by analyzing politeness morpheme {-i-} from the complex form in which it had joined. This paper premise that {-i-} has to be analyzed only when it is helpful to describing the Koren grammar correctly owing to its keeping up its grammatical function, although it had joined with another morpheme diachronially. So this paper concludes that {-i-} cannot be analyzed no more in Modern Korean. It is an important evidence that the function of {-i-} is irregular. For example, even if {-i-} is analyzed identically from both '-seup/eupnida' and '-seup/eupdida', the speech levels of two forms are not same. The former is less polite than the latter. Next, as like the conclusive endings '-ne, -de', '-ni, -di' include {-i-}, but the speech levels of two forms are also different. The former is 'hage-che', on the other hand the latter is 'haera-che'. Because the function of {-i-] is so irregular and unstable like this, it cannot be used for describing Korean grammar synchronically.

GIS와 RS를 이용한 생태지도 작성기법에 관한 기초연구

이기철,이원화,윤해순,남춘희,김구연,김승환,서상현 한국지리정보학회 2004 한국지리정보학회지 Vol.7 No.3

본 연구는 낙동강 하구 일대의 생태계 변화와 생태 관련자료를 GIS DB로 구축하고 생태지도를 작성하였다. 생태지도 작성을 위해 1984년 11월 21일, 1997년 5월 17일에 촬영된 공간해상도 30m의 Landsat TM 위성영상과 국립지리원에서 발행된 1:25,000 수치지형도, 부산시에서 조사한 생물현황 자료를 바탕으로 DB를 구축하였다. 생태지도를 작성하기 위해, 첫째, 낙동강 하구 생태계 조사 보고서와 현재까지 진행된 생태지도에 대한 문헌 연구, 둘째, 시계열적 토지피복분류도 제작, 셋째, 동·식물상, 수질 등 생태계 항목별 BB 구축과 3단계 방식에 의한 생태계 평가, 최종적으로는 이러한 분석 결과를 바탕으로 생태계 관리를 위한 생태지도를 작성하였다. This study developed an ecological mapping technique with GIS database using the analyses of existing ecological survey reports and the change detection on the Nakdong river estuary. The data which are used to establish GIS DB include 2 Landsat TM images on Nov. 31, 1984 and May 17, 1997, 1:25, 000 topographical maps established by National Geography Institution and various ecological survey reports published by Busan metropolitan city government. The details for producing ecological map are as follows. At first, the current methods of ecomapping efforts and previous ecological surveys of Nakdong river estuary were carefully examined. Secondly, the land cover maps were created from the classified Landsat images of 1984 and 1997 for the spatiotemporal ecosystem analysis. Thirdly, the ecosystem was evaluated by using GIS ecological database based on the criteria of botany, zoology and water quality etc. Each criteria was reclassified into 3 stages which describe the overall quality of ecological condition. At last, the comprehensive ecological map was suggested as a prototype of ecosystem assesment and management tool with the discussion of further study. The findings of this study would be a milestone for preserving and managing the ecosystem.

한국어 단모음의 음형 주파수 특성 연구

송철구,윤종락 釜山水産大學校 1994 釜山水産大學 硏究報告 Vol.34 No.1

The formant frequency is a key signature of speech signal and it is a main parameter in speech recognition and synthesis. The object of this study is to characterize and to standardize the formant frequencies of Korean single vowels. The measured people groups consist of man, woman, boy and girl and each group size is 30 persons. The LPC(Linear Predictive Coding) autocorrelation technique is adopted to analyze the formant frequencies and the LPC order is 15 which could be optimum value to figure out the effects of the glottis and the radiation including the vocal tract. The mean values and standard deviation of 1st formant frequeney F1 to 5th formant frequency F5 are obtained and the F1-F2 scatter diagrams of four groups are also presented. The result is compared with Japanese vowel formant frequencies. the overall characteristics is similiar each other but the Korean F2 of vowel/우/is 200 ㎐ lower than that of Japanese. Another finding is that it is difficult to characterize the children's formant frequencies due to their large diversity.

人體에서 分離한 葡萄糖 非醱酵菌의 抗菌劑 耐性에 關한 硏究

李淵台,崔承求,朴哲熙,曺圭鳳 단국대학교 대학원 1991 學術論叢 Vol.15 No.-

This experiment was conducted to classify for the 82 strains of glucose nonfermentative bacteria obtained from K hospital, in 1988, on the biological differentials and to test the resistance reaction on the drugs for those strains. The results were as follows : 1. Eighty two strains were classified into 65 strains of 79.3% of Ps. aeruginosa, 10 strains of 12.2% of Ps. cepacia, and 7 strains of 8.5% of Ac. calcoaceticus as results of 0 F sugar test, oxidase test, and DNase test. 2. It showed that Ps. aeruginosa was coincided with AP, CM, GM, KM, CF, and TC from the disk and MIC methods in the test of resistance reaction to antimicrobial agent, and especially, that AK and TOB KM and GM were observed high resistance reactive from MIC method. Resistnace to antibiotics for Ps. cepacia showed the identical reaction to AP, CB, CM, AK, SPT, CF, TC, TOB, KM and GM were observed high resistance reative from MIC method. Ac. calcoaceticus was identified the same resistance reaction to antibiotics to CB, KM, GM, AK, CF, and TOB, AP, SPT and TC showed high resistance reaction from the disk method. 3. CF and AP were observed the highest resistance reactions among 10 kinds of drugs used in this experiment and following was CM, KM and TC in order. 4. Ps. aeruginosa, Ps. cepacia, and Ac. calcoaeticus showed all multiple resistance reactions in the test of resistance to drugs, and Ps. aeruginosa was identified high resistance reaction to 6 drugs in 24.6%, Ps. cepacia was 9 drugs in 60%, and Ac. calcoaceticus showed resistance reaction to various kinds of drugs. 5. It showed important question in result of these studies that resistance percentage of NFB decreased by optimal antimicrobial selection and effective treatment established from serious antimicrobial treatment.

외상성 뇌경막외 및 뇌경막하 혈종에서 혈종제거술 후 발생한 원발 병소 반대측 혈종에 대한 임상적 고찰

전은오,이상구,전영일,유찬종,김영보,박철완 최신의학사 2003 最新醫學 Vol.46 No.9-10

돈분뇨의 토양처리시 이온 성분의 용탈 특성

김태헌,류성필,김성수,오윤근,허철구 한국환경과학회 2003 한국환경과학회지 Vol.12 No.3

An agricultural land application of swine slurry is one of the best management practices in Jeju island whose ground water must be protected. So as to study the effect of appling swine slurry on ground water or aquifer, incubation-leaching technique was used by assuming the incubating period of 1, 2, 4, 8, 16, or 32 days, and application rate of 3200.0㎎T-P/ℓ, 820.0㎎T-P/ℓ, and 1887.0㎎K^+/ℓ in swine slurry. The leachates were collected from the soil columns(PVC 30㎝L×5.5㎝D) packed 15㎝ in depth with Gangjeong soil series by washing with 100mL distilled water. The leached components were measured by using Ion chromatography for Cl^-, NO_3-N, F^-,Br^-, Na^+, K^+, Ca^2+, and Mg^2+, atomic absorption spectrophotometry for Fe and Mn, and UV-Vis spectrophotometry for T-N and T-P. Application of swine slurry in naked soil could influence on the ground water or aquifer by increasing nitrate-nitrogen in leachate with time, or leaching the cations present in soils in accompany with anions because of H^+ produced in nitrification. Therefore, careful consideration should be taken about what amount, when, where, and how for protecting ground water system.