Profiling Gene Expression During Gland Morphogenesis of a Glanded and a Glandless Upland Cotton

Ying-Fan Cai,Min Chen,Quan Sun,Yong-Fang Xie,Sheng-Wei Li,Jian-Chuan Mo,Ming-Feng Jiang,You-Lu Yuan,Yu-Zhen Shi,Huai-Zhong Jiang,Zheng Pan,Yun-Ling Gao,Peng-Sheng Ye,Hua-Lan Zeng 한국식물학회 2009 Journal of Plant Biology Vol.52 No.6

The pigment gland is an important character of the Gossypium plant. With the aim of identifying genes involved in pigment gland morphogenesis in cotton, gene expression during pigment gland morphogenesis in Chuan 2802, which is glanded both in seed and plant, and a glandless line N5 was profiled using Affymetrix Cotton microarray. The results showed that there were 564 differentially expressed genes greater than twofold during gland morphogenesis. About 60.2% of these genes shares similarity with known genes on GenBank and about 39.8% with no functional description in the database. These described genes may play roles in defense response, response to oxidative stress, peroxidase activity, and the other metabolic pathways. The KEGG Orthology-Based Annotation System indicated that these above twofold expressed genes involved seven biochemical pathways on KEGG. These findings suggest that a complicated regulation is associated with pigment gland morphogenesis and the associated defense response including gossypol biosynthesis in cotton.

Differential Protein Expression Profile Between CD20 Positive and Negative Cells of the NCI-H929 Cell Line

Geng, Chuan-Ying,Liu, Nian,Yang, Guang-Zhong,Liu, Ai-Jun,Leng, Yun,Wang, Hui-Juan,Li, Li-Hong,Wu, Yin,Li, Yan-Chen,Chen, Wen-Ming Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.11

At present, multiple myeloma (MM) remains an incurable disease and cologenic cells may be responsible for disease relapse. It has been proposed that CD20+/CD138- NCI-H929 cells could be hallmarks of MM clonogenic cells. Here, the immunology phenotype of NCI-H929 cells is described. Only a small population of CD20+/CD138- cells (<1%) was found in the NCI-H929 cell line, but CD20+/CD138- cells were not detected. We found that CD20+/CD138+ cells were able to exhibit cologenic capacity by colony formation assay and continuous passage culture. Proteins were analyzed by 1D-SDS-PAGE and TMT based quantitative differential liquid chromatography tandem mass spectrometry (LC-MS/MS). 1,082 non-redundant proteins were identified, 658 of which were differentially expressed with at least a 1.5-fold difference. 205 proteins in CD20+ cells were expressed at higher levels and 453 proteins were at lower levels compared with CD20- cells. Most proteins had catalytic and binding activity and mainly participated in metabolic processes, cell communication and molecular transport. These results proved that there are different biological features and protein expression profile between CD20+ and CD20- cells in the NCI-H929 cell line.

Inhibitory Effects of Syk Transfection on Lung Cancer Cell Invasion

Peng, Chuan-Liang,Zhang, Ying,Sun, Qi-Feng,Zhao, Yun-Peng,Hao, Ying-Tao,Zhao, Xiao-Gang,Cong, Bo Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5

Objective: Spleen tyrosine kinase (Syk) is closely related to tumor invasion and metastasis, and has been shown to have potential inhibitory effects in tumors. In this study, we constructed a eukaryotic expression vector for Syk and analyzed its effects on invasive ability of the A549 non-small cell lung cancer cell line in vitro. Methods: A fragment of Syk was obtained by RT-PCR from human lung cancer cells and cloned into the expression vector pLNCXSyk. After restriction endonuclease digestion, PCR and DNA sequencing confirmation, the recombinant Syk expression plasmid was transfected into A549 human lung cancer cells using lipofectamine protocols. After selection, the cells stably expressed Syk. Detection of Syk expression of the cells by RT-PCR, and invasive ability were examined. Results: The eukaryotic expression plamid pLNCXSyk was constructed and expressed stably in the A549 human lung cancer cells. The RT-PCR results showed that Syk mRNA expression was upregulated significantly (P<0.05). Lower invasion through a basal membrane were apparent after transfection (P<0.05). Conclusions: A eukaryotic expression plasmid to cause Syk expression in lung cancer cells can obviously inhibit their invasive ability in vitro.

Roles of mTOR and p-mTOR in Gastrointestinal Stromal Tumors

Li, Jun-Chuan,Zhu, Hong-Yu,Chen, Ting-Xuan,Zou, Lan-Ying,Wang, Xiao-Yan,Zhao, Hui-Chuan,Xu, Jun Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

Objective: This study aimed to examine the relationship between expression of mammal target of rapamycin (mTOR) and phosphorylation of mTOR (p-mTOR) protein in the PI3K/Akt/mTOR signaling pathways in gastrointestinal stromal tumors and relatiuonships with clinical factors. Methods: Immunohistochemistry was used to detect the expression of the associated proteins mTOR, p-mTOR, and phosphorylation of the tumor suppressor genes PTEN, P27, VEGF, and EGFR in 40 cases of gastrointestinal stromal tumors, with division into a very low and low risk group as well as a moderate and high risk group. Results: The positive rate of mTOR and p-mTOR was significantly increased in the moderate and high risk group compared with the very low and low risk group. The difference was statistically significant (P<0.05). When grouped according to size, the positive mTOR expression rate exhibited a statistical difference (P<0.05), which was significantly increased in the group of tumors larger than 5 cm. The difference in the positive mTOR and p-mTOR expression rate exhibit no statistical significance among the PTEN, P27, VEGF, and EGFR expression subgroups (P>0.05). Conclusion: The different expressions of mTOR and p-mTOR in the signal transduction pathway of gastrointestinal stromal tumor in the different degree-of-risk groups suggested that the mTOR and p-mTOR of the signal transduction pathway serve an important function in the occurrence and development of gastrointestinal stromal tumors.

Combined Assessment of Serum Alpha-Synuclein and Rab35 is a Better Biomarker for Parkinson’s Disease

Hung-Li Wang,Chin-Song Lu,Tu-Hsueh Yeh,Yu-Ming Shen,Yi-Hsin Weng,Ying-Zu Huang,Rou-Shayn Chen,Yu-Chuan Liu,Yi-Chuan Cheng,Hsiu-Chen Chang,Ying-Ling Chen,Yu-Jie Chen,Yan-Wei Lin,Chia Chen Hsu,Huang-Li 대한신경과학회 2019 Journal of Clinical Neurology Vol.15 No.4

Background and Purpose It is essential to develop a reliable predictive serum biomarker for Parkinson’s disease (PD). Te accumulation of alpha-synuclein (αSyn) and up-regulated expression of Rab35 participate in the etiology of PD. Te purpose of this investigation was to determine whether the combined assessment of serum αSyn and Rab35 is a useful predictive biomarker for PD. Methods Serum levels of αSyn or Rab35 were determined in serum samples from 59 sporadic PD patients, 19 progressive supranuclear palsy (PSP) patients, 20 multiple system atrophy (MSA) patients, and 60 normal controls (NC). Receiver operating characteristics (ROC) curves were calculated to determine the diagnostic accuracy of αSyn or/and Rab35 in discriminating PD patients from NC or atypical parkinsonian patients. Results The levels of αSyn and Rab35 were increased in PD patients. The serum level of Rab35 was positively correlated with that of αSyn in PD patients. Compared to analyzing αSyn or Rab35 alone, the combined analysis of αSyn and Rab35 produced a larger area under the ROC curve and performed better in discriminating PD patients from NC, MSA patients, or PSP patients. When age was dichotomized at 55, 60, 65, or 70 years, the combined assessment of αSyn and Rab35 for classifying PD was better in the group below the cutof age than in the group above the cutof age. Conclusions Combined assessment of serum αSyn and Rab35 is a better biomarker for discriminating PD patients from NC or atypical parkinsonian patients, and is a useful predictive biomarker for younger sporadic PD patients.

Identification of two putative phospholipid hydroperoxide glutathione peroxidase genes and the induction of three environmental stresses in Neoseiulus barkeri (Acari: Phytoseiidae)

Chuan Bei Tian,Guo Hao Zhang,Ya Ying Li,Huai Liu 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.1

Phospholipid hydroperoxide glutathione peroxidase (PHGPX) is an antioxidant enzyme that plays a crucial role in metabolizing phospholipid hydroperoxides in membrane against oxidative damage. Here, two PHGPX genes fromthe predatory mite Neoseiulus barkeri were identified and characterized (NbPHGPX1 and NbPHGPX2). Alignment analysis of NbPHGPX1 and NbPHGPX2 showed a great deal of similarity with other known PHGPXs from databases. The well-conserved regions, NVASXCGXT, FPCNQFXXQEP, and IKWNFXKFLV, were surrounded by reactive cysteine, glutamine, and tryptophan residues, respectively. The results of quantitative polymerase chain reaction at different stages of the N. barkeri life cycle showed both NbPHGPX1 and NbPHGPX2 genes were highly expressed in male adults, and their levels of expression were determined upon challenge by different triggers. The data showed that the expression of NbPHGPX1 could be induced by low(4 °C) and high (42 °C) temperatures, UV-B, pyridaben and fenpropathrin,whereas NbPHGPX2 could be only up-regulated by high (42 °C) temperature and fenpropathrin. These results suggested that NbPHGPX1 and NbPHGPX2 genesmight participate in protection of the organism from the oxidative damage challenge by oxidative stresses.

외국문헌초록(外國文獻草綠) : UASB와 MBR의 결합에서 손쉬운 질산화, 탈질화 및 메탄형성의 통합

Ying Yu An,Feng Lin Yang,Hwee Chuan Chua,Fook Sin Wong,Bing Wu 한국수처리학회 2009 한국수처리학회지 Vol.17 No.2

Development of human IgE biosensor using Sezawa-mode SAW devices

Ying-Chung Chen,Wei-Tsai Chang,Chien-Chuan Cheng,Jing-Yi Shen,Kuo-Sheng Kao 한국물리학회 2014 Current Applied Physics Vol.14 No.4

This paper reports Sezawa-mode surface acoustic wave (SAW) devices with via-isolated cavity to construct the allergy biosensor. To fabricate Sezawa-mode SAW devices, the RF magnetron sputtering method for the growth of piezoelectric ZnO thin films are adopted and influences of the sputtering parameters are investigated. The optimal substrate temperature of 300 C, RF power of 120 W and sputtering pressure of 2 Pa were used to deposit piezoelectric ZnO films with a smooth surface, uniform grain size and strongly c-axis-orientated crystallization. A back-etched SAW resonator is used in this study. The wet etching of (100)-oriented silicon wafers is used to form a back-side cavity which is critical to the formation of a hopper cavity for holding bio-analytes. The remaining membrane structure silicon thickness was 25 mm. In this report, the chrome (Cr, 12 nm)/gold (Au, 66 nm) layer was initially deposited onto the sensing area of SAW devices as the binding layer for biochemical sensor. The resonance frequency of the Sezawa-mode SAW device is 1.497 GHz. The maximum sensitivity of the Sezawa-mode is calculated to be 4.44 106 cm2/g for human immunoglobulin-E (IgE) detection. The stability for human IgE detection is calculated to be 80% and the variation of the stability 3% was obtained after several tests.

Strain Rate Effects on the Mechanical Properties of an AlCoCrFeNi High-Entropy Alloy

Chuan Ting Wang,Yong He,Zhiping Guo,Xiaohui Huang,Ying Chen,Houan Zhang,Yuan He 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.7

The efect of strain rate on the compressive properties of an AlCoCrFeNi high-entropy alloy (HEA) was investigated. Microstructure of the AlCoCrFeNi HEA was observed using scanning transmission microscopy (SEM) and transmission electronmicroscopy. The results showed formation of a homogeneous two-phase structure. Quasi-static compression was testedunder initial engineering strain rates between 10–4 and 10–2 s−1. Engineering compression stress of exceeding 2500 MPaand ductility of around 12% was achieved. Dynamic mechanical behavior at room temperature was characterized by a splitHopkinson pressure bar under strain rates between 1350 and 4000 s−1. The AlCoCrFeNi HEA exhibited high strain ratesensitivity, especially under dynamic compression. The fuctuation of yield strength and the variation of strain rate hardening with strain rate was investigated. The various parameters of a constitutive equation for deformation of the AlCoCrFeNiHEA were obtained from the experimental data. The constitutive equations can be applied to predict the strength of alloyunder various stain rates.

Ce4+-Stimulated Ion Fluxes Are Responsible forApoptosis and Taxol Biosynthesis in Suspension Cultures of Taxus Cells

Ying-Jin Yuan,Jing-Chuan Li,Zhi-Qiang Ge 한국생물공학회 2005 Biotechnology and Bioprocess Engineering Vol.10 No.2

Ion fluxes across the plasma membrane activated by 1 mM Ce4+, cell apoptosis and taxol biosynthesis in suspension cultures of Taxus cuspidata were studied. The extracellular pH sharply decreased upon the addition of 1 mM Ce4+, then increased gradually and exceeded the initial pH value over a time period of 12 h. The extracellular Ca2+ concentration decreased within the first 3 h after the addition of Ce4+, then gradually decreased to one third of initial value in control at about 72 h and remained unchanged afterwards. Experiments with an ion channel blocker and a Ca2+-channel blocker indicated that the dynamic changes in extracellular pH and the Ca2+ concentration resulted from the Ce4+-induced activation of H+ uptake and Ca2+ influx across the plasma membrane via ion channels. A pretreatment of the ion channel blocker initiated Ce4+-treated cells to undergo necrosis, and the prior addition of the Ca2+-channel blocker inhibited Ce4+-induced taxol biosynthesis and apoptosis. It is thus inferred that H+ uptake is necessary for cells to survive a Ce4+-caused acidic environment and is one of the mechanisms of Ce4+-induced apoptosis. Furthermore, the Ca2+ influx across the plasma membrane mediated both the Ce4+-induced apoptosis and taxol biosynthesis.