Morphological, Phylogenetic and Biological Characteristics of Ectropisobliqua Single-Nucleocapsid Nucleopolyhedrovirus

Chuan-Xi Zhang,Hai-Jun Xu,Mei-Jun Tang,Qiang Xiao,Jian Hong,Xiu-cui Ma 한국미생물학회 2006 The journal of microbiology Vol.44 No.1

The tea looper caterpillar, Ectropis obliqua, is one of the major pests of tea bushes. E. obliqua single-nucleocapsid nucleopolyhedrovirus (EcobSNPV) has been used as a commercial pesticide for biocontrol of this insect. However only limited genetic analysis for this important virus has been done up to now. EcobSNPV was characterized in this study. Electron microscopy analysis of the occlusion body showed polyhedra of 0.7 to 1.7 μm in diameter containing a single nucleocapsid per envelope of the virion. A 15.5 kb genomic fragment containing EcoRI-L, EcoRI-N and HindIII-F fragments, was sequenced. Analysis of the sequence revealed that the fragment contained eleven potential open reading frames (ORFs): lef-1, egt, 38.7k, rr1, polyhedrin, orf1629, pk-1, hoar and homologues to Spodoptera exigua multicapsid NPV (SeMNPV) ORFs 15, 28, and 29. Gene arrangement and phylogeny analysis suggest that EcobSNPV is closely related to the previously described Group II NPV. Bioassays on lethal concentration (LC50 and LC90) and lethal time (LT50 and LT90) were conducted to test the susceptibility of E. obliqua larvae to the virus.

The utilization and industrialization of insect resources in China

Chuan-Xi Zhang,Xu-Dong TANG,Jia-An CHENG 한국곤충학회 2008 Entomological Research Vol.38 No.-

Insecta is the biggest group of animals on earth. The insects are thought to be one of the biggest biological resources that have not been fully exploited by humans. China is one of the earliest countries to exploit insect resources in the world and has been the top producer for over one thousand years of many insect-related industrial products, such as silk, insect wax and Chinese gallnuts. The exploitation and industrialization of insect resources in China is generally classified into four different levels. The first level is the direct utilization of insect bodies and their secretions, the history of which can be traced back for thousands of years. This level includes the culture and utilization of the silkworm Bombyx mori, the Chinese honeybee Apis cerana cerana, the Chinese white-wax scale Ericerus pela, the Chinese gall aphid Schlechtendalia chinensis, and the lac insects Kerria spp. Additionally, numerous other insects are typically used for Chinese traditional medicines and food, such as Eupolyphaga sinensis, Opisthoplatia orientalis, Aspongopus chinensis, Martianus dermestoides, Polyrhachis vicina, Hepialus spp, Vespa, Hydrillodes repugnalis, and Tenebrio molitor. Pollinators (Megachile rotundata, Osmia cornifrons, O. excavata) and ornamental insects like butterflies, katydids Gampsocleis grafiosa, and fighting crickets Scapsipedes micado are also among the insects included in this level. Accordingly, a related industry is insect-breeding, including sericulture and apiculture, which lays the basis for all insect industrialization. The second level is the utilization of insects as enemies of pests and insect pathogens for biological control. The enemy insects, including the egg parasites tricogramma Trichogramma spp, the seven spotted lady beetle Coccinella septempunctata, the Chinese green lacewing Chrysopa sinica, and Anastatus sp. could be produced in large scale. The insect pathogens that have been extensively used for commercial biocontrol in China include Helicoverpa armigera Nucleopolyhedrovirus (HaSNPV), Ectropis oblique Nucleopolyhedrovirus (EcobNPV), Spodoptera exigua multicapsid nucleopolyhedrovirus (SeMNPV), Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV), Plutella xylostella Granulosis (PxGV), Pieris rapae granulosis (PiraGV), and Bacillus thuringiensis (Bt). Related industries include the biopesticide industry and the enemy insect production industry. The third level of utilization is the extraction and synthesis of insect materials with diverse bioactivities. Some insect pheromones and hormones extracted from insect bodies or chemically synthesized have been used for insect pest control and for regulating the silkworm breeding. Toxins from honeybees and wasps have been used in medicine. Some insect materials from the larvae of honeybees, silkworms, tasar silkworms, and houseflies have been developed into health products. The fourth level is using the insects as bioreactors to produce peptides for medical and veterinary uses. Hundreds of foreign genes have been successfully expressed in the insect cells and larvae. The hGM-CSF expressed in silkworm pupae is commercially available. In this article, we review the culture and utilization of important industrial insects in China.

Characterization of Ha29, a Specific Gene for Helicoverpa armigeraSingle-nucleocapsid Nucleopolyhedrovirus

Chuan-Xi Zhang,Zhong-Jian Guo,Shi-Heng An,Dun Wang,Yan-He Liu,V. Shyam Kumar 한국생화학분자생물학회 2005 BMB Reports Vol.38 No.3

Open reading frame 29 (ha29) is a gene specific for Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus (HearSNPV). Sequence analyses showed that the transcription factor Tfb2 motif, bromodomain and Half-A-TPR (HAT) repeat were present at aa 66-82, 4-76, 55-90 of the Ha29 protein respectively. The product of Ha29 was detected in HearSNPV-infected HzAM1 cells at 3 h post-infection. Western blot analysis using a polyclonal antibody produced by immunizing a rabbit with purified GST-Ha29 fusion protein indicates that Ha29 is an early gene. The size of Ha29 product in infected HzAM1 cells was about 25 kDa, which was larger than the presumed size of 20.4 kDa. Tunicamycin treatment of HearSNPV-infected HzAM1 cells suggested that the Ha29 protein is N-glycosylated. Fluorescent confocal laser scanning microscope examination, and Western blot analysis of purified budded virus (BVs), occlusion-derived virus (O DVs), cell nuclear and cytoplasmic fraction, showed that the Ha29 protein was localized in the nucleus. Our results suggested that ha29 of HearSNPV encodes a non-structurally functional protein that may be associated with virus gene transcription in Helicoverpa hosts.

Deep Leaky Single-peaked Triangle Neural Networks

Chuan-Hui Shan,Xi-Rong Guo,Jun Ou 제어·로봇·시스템학회 2019 International Journal of Control, Automation, and Vol.17 No.10

Recently, Deep learning has made a great deal of success in processing images, audios, and natural languages and so on. The activation function is one of the key factors in Deep learning. In this paper, according to characteristics of biological neurons, an improved Leaky Single-Peaked Triangle Linear Unit (LSPTLU) activation function is presented for the right-hand response unbounded of Rectified Linear Unit (ReLU) and Leaky ReLU(LReLU). LSPTLU is more in line with the biological neuron essence and achieves the excellent performance of equivalent or beyond ReLU and LReLU on different datsets, e.g., MNIST, Fashion-MNIST, SVHN, IMAGENET, CALTECH101 and CIFAR10 datasets.

LSKL, a Peptide Antagonist of Thrombospondin-1, Attenuates Renal Interstitial Fibrosis in Rats with Unilateral Ureteral Obstruction

Xi-sheng Xie,Fei-yan Li,Heng-chuan Liu,Yao Deng,Zi Li,Jun-ming Fan 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.2

The effects of LSKL, the peptide antagonist of thrombospondin-1 (TSP-1), on renal interstitial fibrosis in rats subjected to unilateral ureteral obstruction (UUO) were investigated. Rats were divided randomly into three groups (n = 20 each): UUO group, sham-operation group and UUO plus LSKL treatment group. Collagen deposition was studied using histopathology and reverse transcription polymerase chain reaction analysis (RT-PCR). TSP-1, transforming growth factor beta 1 (TGF-β1), phosphorylated Smad2 (pSsmad2) and α-smooth muscle actin (α-SMA) in the kidney were measured using immunocytochemistry, western blotting analysis, RT-PCR and enzyme-linked immunosorbent assay. Biochemical analyses in the serum and urine were made. Histopathology showed severe tubular dilatation and atrophy, interstitial inflammation and collagen accumulation after surgery and LSKL significantly inhibited interstitial fibrosis including tubular injury as well as collagen deposition. The protein and mRNA levels of TSP-1 increased notably at different time point and significantly decreased in the presence of LSKL. The expression of TGF-β1 and pSmad2 were upregulated in the obstructed kidney and substantially suppressed by LSKL treatment. Myofibroblast accumulation could be alleviated after administration of LSKL. Biochemical parameters did not show differences among the three groups. As TSP-1 is the major activator of TGF-β1, we demonstrate that LSKL can attenuate renal interstitial fibrosis in vivo by preventing TSP-1-mediated TGF-β1 activation.

Pretreatment with Trichoderma sp. AH promotes directional conversion of organic nitrogen compounds in supercritical methanolysis of wheat straw

Quan-Xi Zheng,Chuan-Yong Yan,Mu-Wei Zhu 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.128 No.-

The specificity and conversion of supercritical methanolysis of wheat straw (WS) and rice straw (RS) canbe enhanced by Pretreatment using Trichoderma sp. AH, particularly the esters of the methanol-solubleportion (MSP) of WS and RS in supercritical methanolysis. However, only about 100 organic compoundsof lower volatility and polarity could be detected in MSP by gas chromatography-mass spectrometry. Electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry was used to determinethe organic nitrogen compounds (ONCs) in the MSPs from supercritical methanolysis of raw andpretreated WS with Trichoderma sp. AH. The most prevalent ONCs in the MSPs of native and pretreatedWSs in the positive ion mode would be N2On (n = 1–9) species having twin-bonding equivalence values(2–25) together with carbon-atom numbers of 4–35. Peak prevalent ONC within prior-treated WS MSPs isN2O4, with the most abundant ONC in raw WS MSPs is N2O5. These results help to understand the impactof Trichoderma sp. AH pretreatment upon converting ability/specificity for ONCs within WS methanolysisand employing MSP in producing jet fuel.

Chloroquine and Valproic Acid Combined Treatment in Vitro has Enhanced Cytotoxicity in an Osteosarcoma Cell Line

Wang, Chuan-Kun,Yu, Xi-Dong,Li, Qiang,Xie, Gang,Teng, Yue Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.8

Choroquine (CQ) and valproic acid (VPA) have been extensively studied for biological effects. Here, we focused on efficacy of combined CQ and VPA on osteosarcoma cell lines. Viability of osteosarcoma cell lines (U20S and HOS) was analyzed by MTT assay. Apoptotic assays and colony formation assays were also applied. ROS generation and Western Blotting were performed to determine the mechanism of CQ and VPA combination in the process of apoptosis. The viability of different osteosarcoma cell lines significantly decreased after CQ and VPA combination treatment compared with either drug used alone, and apoptosis was increased significantly. ROS generation was triggered leading to expression of apoptosis related genes being increased and of antiapoptotic related genes being decreased. From our data shown here, CQ and VPA combination treatment in vitro enhanced cytotoxicy to osteosarcoma cells.

Mitigation of Sub-synchronous Oscillation Caused by Thyristor Controlled Series Capacitor Using Supplementary Excitation Damping Controller

Wu, Xi,Jiang, Ping,Chen, Bo-Lin,Xiong, Hua-Chuan Journal of International Conference on Electrical 2012 Journal of international Conference on Electrical Vol.1 No.2

The Test Signal Method is adopted to analyze the impact of thyristor controlled series capacitor (TCSC) on sub-synchronous oscillation. The results show that the simulation system takes the risk of Sub-synchronous Oscillation (SSO) while the TCSC is operating in the capacitive region. A supplementary excitation damping controller (SEDC) is used to mitigate SSO caused by the TCSC. A new optimization method which is aimed for optimal phase compensation is proposed. This method is realized by using the particle swarm optimization (PSO) algorithm. The simulation results show that the SEDC designed by this method has superior suitability, and that the secure operation scope of the TCSC is greatly increased.

Many-to-One Encryption and Authentication Scheme and Its Application

Lin, Xi-Jun,Wu, Chuan-Kun,Liu, Feng The Korea Institute of Information and Commucation 2008 Journal of communications and networks Vol.10 No.1

This paper is to study a subclass of group-oriented cryptographic scheme: Many-to-one encryption and authentication scheme. The many-to-one encryption and authentication scheme is to solve a practical problem, i.e., the scenario that the number of the receivers is very small compared with the number of the senders and a receiver may serve millions of senders. Compared with the traditional methods, the burdens of the receiver and the KGC are reduced greatly. How to revoke a sender from his receiver's legitimate sender group is also proposed and it is efficient compared with some traditional methods. The proposed scheme is proven in the random oracle models. The computational complexity of our scheme is independent of the number of the senders. At the end of the paper, an example is given to show how to use our scheme in online software registration and update.

BmNPV chitinase gene deletion enhances foreign gene expression in a BmN cell system

Fang WANG,Chuan-Xi ZHANG 한국곤충학회 2009 Entomological Research Vol.39 No.1

Bombyx mori nucleopolyhedrovirus (BmNPV) is extensively being studied as an expression vector for heterologous gene expression in silkworm-derived cells as well as in the host larvae or pupae. BmNPV chitinase is necessary for liquefaction of the virus-infected host insect. The influence of chitinase on the efficiency of foreign gene expression was studied to provide a scientific basis for improving the BmNPV expression system. The BmNPV chitinase gene (chiA) was deleted and the expression level of the polyhedrin promoter controlling the lacZ gene in BmN cells was determined. Sodium dodecylsulfate (SDS)–olyacrylamide gel electrophoresis (PAGE) showed that β-galactosidase accounted for approximately 6.9 and 7.7% of the total protein in BmN cells infected with the chiA deficient Bm lacZ+chiA−,at 3 and 4 days post infection, while the total protein was 3.2 and 4.2% in cells infected with Bm lacZ+. The relative β-galactosidase activities in Bm lacZ+chiA−[기호] -infected cells improved 2.33- and 1.56-fold compared to those of Bm lacZ+-infected cells at 3 and 4 days post infection. The results of the present study suggest that chitinase deletion could improve the lacZ expression level in the BmNPV-BmN cell expression system.