Comparison of Proliferative Activity in Each Histological Subtypes of Benign and Atypical Intracranial Meningiomas by PCNA and Ki-67 Immunolabeling

최승진,장은덕,권성오,계대곤,박춘근,이상원,강준기,Choi, Seung Jin,Chang, Eun Deok,Kwon, Seung Oh,Kye, Dae Kon,Park, Choon Keun,Lee, Sang Won,Kang, Joon Ki The Korean Neurosurgical Society 2000 Journal of Korean neurosurgical society Vol.29 No.9

목 적 : 양성 뇌수막종에 비하여 이형성 및 악성 뇌수막종이 나쁜 임상적인 예후 및 양상을 보이는 것은 잘 알려져 있으나, 양성 뇌수막종에 있어서 각각의 병리조직학적 아형에 따른 생물학적 양상의 차이에 대해서는 잘 알려지지 않거나 일부 논란이 되고있다. 본 연구에서는 이형성 뇌수막종 및 양성 뇌수막종의 각각의 병리조직학적 아형에 따른 증식능의 차이여부를 알아보고자 PCNA와 Ki-67표지지수를 분석하였다. 방 법 : 본원에서 뇌수막종으로 수술을 시행하여 얻은, 재발을 보여 재수술을 시행한 2례를 포함하여, 파라핀에 포매시킨 27개의 조직을 대상으로 병리학적인 증식능을 분석하기 위해, PCNA에 대한 단일항체 및 MIB-1 단일항체를 이용한 면역조직화학적 염색을 시행하였다. 조직학적 분류상 meningothelial type이 8례, transitional type이 9례, fibroblastic type이 5례였으며, 이형성 수막종이 5례였다. 결 과 : PCNA표지지수의 평균값은 양성 수막종에서 meningothelial type이 $4.82{\pm}5.10%$, transitional type이 $9.01{\pm}4.25%$, fibroblastic type이 $5.66{\pm}5.32%$를 보였으나 이형성 수막종에서는 $27.62{\pm}19.67%$의 높은 지수를 나타냈고, Ki-67 표지지수의 평균값은 양성 수막종의 아형에서 각각 $0.43{\pm}0.85%$, $0.44{\pm}1.08%$, $0.24{\pm}0.18%$를 보이고, 역시 이형성 수막종에서는 $0.84{\pm}0.59%$의 높은 지수를 보였다. 즉, 양성 수막종에서 각각의 아형에 따른 PCNA 및 Ki-67 표지지수는 통계학적으로 의미있는 차이는 없었으나(p>0.05), 이형성 수막종에서는 의미있는 높은 표지지수를 보여(p<0.05) 양성 수막종에서 보다 높은 증식능을 보임을 알 수 있었다. 결 론 : PCNA 및 Ki-67 표지지수를 이용한 증식능의 비교결과, 양성 뇌수막종에서는 각각의 아형에 따른 생물학적 양상이나 예후는 차이가 없을것으로 생각되나, 이형성 수막종에서는 높은 증식능을 보여 이에 대한 예후를 예상할 수 있을것으로 생각되며, 또한 이러한 표지지수가 병리조직학적으로 양성과 이형성의 감별에 많은 도움이 될것으로 사료된다. Objective : The clinical prognosis and biological behavior of atypical and especially malignant meningiomas are well known to be worse than benign meningioma, but the degree of biological aggressiveness in each classical subtypes of benign meningioma is controversy. This study was performed to see whether there is a difference in the proliferative activity between each different histological subtypes of benign meningioma as well as atypical meningioma. Methods : Paraffin-embedded surgical specimens of 27 meningiomas, including two recurrent tumors, were studied to evaluate proliferative activity by immunohistochemical method with monoclonal antibodies to proliferating cell nuclear antigen(PCNA) and MIB-1. The specimens consisted of 8 cases of meningothelial, 9 cases of transitional, 5 cases of fibroblastic subtypes and 5 cases of atypical meningiomas. Results : Mean PCNA labeling indices of meningothelial, transitional and fibroblastic meningiomas were $4.82{\pm}5.10%$, $9.01{\pm}4.25%$ and $5.66{\pm}5.32%$, but that of atypical meningiomas was $27.62{\pm}19.67%$, noting a higher value compared to all three subtypes of benign meningiomas. Mean Ki-67 labeling indices of the above 3 subtypes were $0.43{\pm}0.85%$, $0.44{\pm}1.08%$ and $0.24{\pm}0.18%$, and that of atypical meningiomas was also revealed to be of higher value ($0.84{\pm}0.59%$). PCNA and Ki-67 labeling indices were not statistically different between histological subtypes of benign meningioma(p>0.05), but the differences of both immunolabeling between benign and atypical meningiomas were statistically significant(p<0.05). Conclusion : Immunolabeling of PCNA and Ki-67 in intracranial meningiomas reveals no prognostic difference between meningothelial, transitional and fibroblastic subtypes in classical benign meningiomas by measuring expression of PCNA and Ki-67, but it seems to be helpful in differentiating benign and atypical meningioma, later showing more proliferative activity and biological aggressiveness.

음곡 상응부위에 시술한 방기 약침이 LPS로 유도된 흰쥐의 염증성 신손상에 미치는 영향

김기춘 ( Ki Choon Kim ),임윤경 ( Yun Kyoung Yim ) 경락경혈학회 2013 Korean Journal of Acupuncture Vol.30 No.4

Objectives: This study aimed to evaluate the effects of Stephaniae tetrandrae Radix Herbal-acupuncture (SR-HA) at KI10 (Umgok) on nephritis induced by lipopolysaccharide (LPS) in rats. Methods: Male SD rats (n=32) were allocated to four groups; normal, LPS, saline, and SR-HA. The rats in the LPS, saline, and SR-HA groups were injected intra-peritoneally with LPS (2 mg/kg) to induce nephritis. The rats in the saline and SR-HA groups were treated with saline injection and SR-HA respectively at KI10 three times a week. To evaluate the effects of SR-HA at KI10 on nephritis in rats, the authors evaluated the numbers of WBC and neutrophils in blood, and the levels of creatinine, TNF-α, CINC-1 in serum, creatinine, total protein in urine and renal MPO. Results: SR-HA at KI10 significantly inhibited the increase of WBC and neutrophils in blood, TNF-α, CINC-1 in serum, creatine, total protein in urine and MPO in kidney of LPS-stimulated rats. Conclusions: SR-HA at KI10 may have therapeutic effects on nephritis in LPS-stimulated rats.

한국 근대 자유시의 미적 전략과 근대적 서정의 형식

노춘기(Noh, Choon-Ki) 한국시학회 2015 한국시학연구 Vol.- No.44

The purpose of this article is investigation of the formation and the esthetic attitude of Korean modern free-verse. In 1900’s the poem written in Korean language was recognized as an effective means of enlightenment campaign. But in the respect of subject consciousness and linguistic form, the writers of free-verse showed a different intention point from the senior writers. Since the emergence of the magazine of HakJiKwang, the younger writers presented the pioneering realization of the modern literature and the artistic expression. The modern free-verse in Korean appeared for the first time in the middle of 1910’s. And Hwang, Seok-Woo declared the aesthetic theory of the free-verse as a form of modern poetry in late 1910’s. He insisted that the starting point of Korean modern poetry must be in the free-verse. It meant that the free-verse is the essence of modern poetry. And he stipulated that the word of soul-nonusual and unpopular expression-is the essentials of poetic language. He denied the form of presentation of the previous pattern of poetry as a chantable lyrics. The writers of the freeverse advocated the realistic portrayal of the torturous world and the wounded subject. They defended the modern free-verse and suggested the aesthetic goal for the future of Korean modern poetry.

만성 호중구성 백혈병 1례

기장도,박치영,류보열,김태종,모성환,곽재정,문철호,박근홍,박유환,정춘해 朝鮮大學校 附設 醫學硏究所 1996 The Medical Journal of Chosun University Vol.21 No.1

Chronic neutrophilic leukemia is a very rare myeloproliferative disorder which is characterized by severe sustained mature neutrophilic leukocytosis in peripheral blood (PB), hepatosplenomegaly, elevated leukocyte alkaline phosphatase, serum uric acid. serun viatmin B 12 and the bleeding tendency despite normal platelet count and coagulation time, absence of philadelphia chromosome, and the absence of fever or underlying infection or disease sufficient to mimic a leukemoid reaction. We have experienced a 34-years-old male patient with atypical chronic neutrophilic leukemia. On admission, the leukocyte count was 56,860/uL. severe neutrophic leukocytosis with 76% neutrophils and rare immature forms on PB smear. Many neutrophils had toxic granules, and vacuoloes. Leukocyte alkaline phosphatase score was decreased. The cytogenetic study showed Philadelphia chromosome negative with normal karyotype.

영어 복수화에 나타난 심리적 실체

박춘기 한국영어영문학회 경남지부 1984 현대영미어문학 Vol.2 No.-

This is an attempt to find out the psychological reality of English pluralization as a process structure which is shown by language acquisition, historical change, and loan word. The study of the psychological reality in the language started from Sapir(1933) is mainly approached by phonologists. This pacer, however, covers the psychological reality of syntactic structure as well as phonological structure. In the course of language acquisition, the regular pluralization represented by phonology is achieved before the age of 6. On the other hand, when the children learn irregular rules of pluralization, they show either over-or under-generalization. This means they have psychological reality in their language. In the historical change, the regular form, s-ending, originated from-(a)s or -s in Old English has been changed through Middle English. But the mutation in Old English has teen changed into two kinds: one originated from Old English, the other substituted for regular form(e. g., books, oaks, *oats). These regular forms also show the psychological reality of the native speakers. As for loan words, 80 words out of 111 ones in A Book of Contemporary English by Quirk, et. al., adopted the regular rule or tend to follow the regular rule in English. This fact also toindicates that there is psychological reality in English pluralization. With all its psychological reality, it is a question that we simply do not know to what extent linguistic evidences have psychological reality.

운동선수의 연령과 운동자각도에 의한 최대심박수 예측

민춘기,강도욱 忠南大學校體育科學硏究所 1992 體育科學硏究誌 Vol.10 No.1

The purpose of this study was to assumption of maximal heart rate by age and the rating of perceived exertion (RPE) during maximal exercise of 40 male athletes in college. And this study was look at increase the heart rate, age and the rating of perceived exertion (RPE) in order to make an analysis of correlation and make an equation of revolution by these factors. The following conclusions were obtained: 1. The correlation of relation age with heart rate during maximal exercise was a high significant. (r=.0299) 2. The correlation of relations the rating of perceived exertion(RPE) with age by heart rate during maximal exercise was a high significant. (RPE : R= .0034, AGE : R= .0076) 3. The revolutionary equation of maximal heart rate's assumption made as a consequence of HRmax = 108.461(calculation) + 0.5108(RPE15) - 0.6570(8HR) + 0.6075(10HR) - 0.2641(AGE). 4. The confidence was increased 35% in this study rather than in the previously reported (220 - Age) prediction equation of maximal heart rate.

一部 美容業 從事者들의 織責別 腰痛 有病率과 關聯要因

김춘일,박종,류소연,김석산,김기순,하상호,변주남 조선대학교 부설 의학연구소 1999 The Medical Journal of Chosun University Vol.24 No.1

To find the prevalence and related factors of low back pain experienced by some beauticians. a survey was made toward 252 beauticians in Kwangju City from March 1st to April 1st, 1998. The results were as follows; 1. The prevalence rate of low back pain among the respondents for the last week was 59.5%. 2. The aid group and staff group with low back Pain had significantly longer duration of work than those without low back pain. Managers who had bad posture with twisted or too much extended back showed a significantly higher prevalence of low back pain than those with good posture during work(P<05). 3. Aid group with low back pain showed significantly higher points at total stress score, depression score, general health score and vitality score than those without low back Pain. The staff group with low back pain showed significantly higher points for total stress score, sleep disorder and anxiety score, depression score, general well-being and vitality score than those without low back pain. Managers with low back Pain showed significantly higher points of stress at social performance and self confidence (P<05) than those without low back pain. The above results suggest that low back pain is related to different jobs and work environments for beauticians. So effective measures and further studies against risk factors must be made considering their jobs. To prevent or reduce low back pain, appropriate management of stress for beauticians is necessary.

테니스 스트로크 動作에 動員되는 筋肉에 對한 筋電圖的 分析 硏究

朴麟基,田東善,閔春基 忠南大學校體育科學硏究所 1985 體育科學硏究誌 Vol.3 No.1

In this paper, the Electromyogram discharges in the muscles mobilized for ground stokes are measured before and after the impact. From the analysis of the aspect of electrical discharge we obtain the following results. 1. In regard to the upper limb, when the player executes the forehand storoke the strongest aspect of discharge of electricity(ADE)appears flexor carpi ulnaris, while strong secondary ADE appear on the brachialis and biceps brachialis. However, in the execution of the backhand stroke, only a weak ADE appears on the triceps brachialis, followed by a momentary, strong ADE on the flexor carpi ulnaris and brachioraialis. 2. In regard to the muscles around the trunk when the player executes the forehand strokes, the strongest ADE appears on the latissimus dorsi with the secondary, strong ADE the deltoid with the backhand strokes, however, the strongest ADE appears on the vastus major. 3. In regard to the muscles of the lower limb when the player executes the forehand strokes, a strong and durative ADE appears on the biceps femoris and gastrocnemius, both of which are posterior muscles. However, after impact of the strokes, a weak ADE appears on the rectus femoris and the vastus medialis both of which are anterior muscles. When the player executes the backhand strokes the anterior and posterior muscles show opposite aspects in the process of contraction and relaxation.

HMM/VQ에 의한 효율적인 음소인식에 관한 연구

장춘서,이기수,임은기 금오공과대학교 산업기술개발연구원 2000 産業技術開發硏究 Vol.16 No.-

In this paper, an effective phoneme recognition method for large vocabulary speech recognition system has been studied. VQ(Vector Quantization) is used as a front-end system for HMM(Hidden Markov Model) recognizer. Optimum number of phoneme classes and optimum size of code book for phoneme basis recognition environment has been proved by the result of computer simulation.

노인의 운동프로그램이 건강관련 체력에 미치는 효과

민춘기 忠南大學校體育科學硏究所 2003 體育科學硏究誌 Vol.21 No.1

The Purpose of this study was to determine the effects of program for improving health related physical fitness developed for older people by measuring the changes in the participants' muscle strength, muscle endurance, flexibility, cardiovascular fitness. This study employed 36 healthy older people who were 60~65 years old (mean age 62.5years). The subjects were randomly assigned to one of three groups (two experimental groups and one control). Each group ended up with 12 subjects. The results of the study were as follows : 1. The program for improving health related physical fitness program administered to the experimental groups showed statistically significant effects with respect to the positive changes in cardiovascular fitness during the treatment period. 2. The physical fitness program administered to the experimental groups showed statistically significant effects with respect to the positive changes in flexibility. 3. The program for improving health related physical fitness program administered to the experimental groups showed statistically significant effects with respect to the positive changes in musclular endurance during the treatment period. 4. The program for improving health related physical fitness program administered to the experimental groups showed statistically significant effects with respect to the positive changes in musclular strength during the treatment period.