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      • 후박 및 은행엽 추출물의 항균, 항염 및 세포활성도에 미치는 영향

        정종평,구영,배기환 충남대학교 약학대학 의약품개발연구소 1995 藥學論文集 Vol.11 No.-

        Periodontal therapy for treatment of periodontitis involves the elimination of bacterial plaque and elimmation of the anatomic defects by regenerative procedure. The purpose of this study was to evaluate on the biological effect of magnolia and Ginkgo biloba extract to the antimicrobial, antiinflammatory and cellular activity. Antimicrobial assay was performed with the diffusion method of the extract by measuring of growth inhibitory zone of B. cereus from blood agar plate. Effect of the extract to ce11ular activity of gingival fibroblast were examined using MTT method and measured the result with optical density on 570nm by ELISA reader. Inhibitory effects of PGE_2 production from gingival fibroblast was performed with the addition of IL-1β and the extract to the well and examined to the product of PGE_2 from ce1l by ELISA reader. In vivo anti-inflammatory effect was performed with injection examined with clinically and histologically for their extent of mecrosis and inflammation. Antimicrobial activity of Magnolia extract showed significantly higher activity than that of control. However, GBE did not showed significant activity to compare with control, and mixture of Magnolia and GBE extract showed significantly higher activity than that of control. The effect of cellular activity to gingival fibroblast showed no significant differences of between control and Magnolia extract. However, GBE showed significant higher rate of cellular activity to compare with control and even to PDGF-BB, and also showed same degree of cellular activity even though mixed with Magnolia extract. The inhibitory effect of PGE_2 production showed significantly reduction of PGE_2 production to compare with control, but its inhibitory effect was not much strong to compare with Indomethacin. In vivo, antiinflammatory effect of Magnolia extract to P. gingivalis injection of Hamster buccal check showed significantly reduction of inflammatory cell infiltration and tissue necrosis, but GBE showed no effect on the inhibition of inflammatory process. These results suggested that Magnolia and GBE extract possessed different kind of biological activity and also can be compensated on their activity with each other for elimination of bacterial plaque and anatomical defect.

      • 조밀작용성을 가지는 변환군

        주진구,지종선,이동수 충남대학교 자연과학연구소 1978 學術硏究誌 Vol.5 No.2

        The dense action property in topological transformation groups is introduced in this paper. This is a special property of the topological phase group on the phase space in a given transformation group. Some relationships between transformation groups with the dense action property and homomorphisms are described. It is shown that every homomorphism of a transformation group (X, O(n)) onto (S^(n-1)-: O(n)) is open and every homomorphism of (X, SO (n)) onto (S^(n-1), SO (n)) is also open, n>1.

      • Computer Application Program의 運用에 關한 硏究 : Linear Progromming을 中心으로

        박종구 圓光大學校 1983 論文集 Vol.17 No.2

        Mathematical Optimization is any mathematical technique for datermining the optimum use of various resources to attain a particuler objective (such as mimimum cost of maximum profit) when there are alternate use for resources. Iiner programming is the most widely used of these techniques and has been used to allocate, assign, schedule, select, or evaluate the use of limited reseurces for such jobs as blending, mixing, cutting, trimming, bidding, pricing, purchasing, planning, and the transportation and distribution of raw materials and finish products. the purpose of this paper is to develope an alloy blending for the linear propramming. first, model was set up. second, the model was changed to LPS model to formulate into input data Explanation was, therefore, made with respect to Control card and card deck structure in order to identify scope of research on report reading which relate to output of LP solution. In consideration of maket cost changes, an investigation was made with respect to card deck structure, input data, control card and report reading into output in order to formulate into cost change. An investigation was made concerning card deck structure, input data, control card and report reading into output in order to formulate the second LP solution report which utilize the rest part of raw materials after initial alloy blending. the final of main subject (7 and 8) contains a description of the system, the machine configuration requred, and limitson problem size. in this research, we provide a discussion of a basis for the development of a LPS model. Furthermore, an attempt was made to approach both developed and undeveloped program which could contribute to the new LP user.

      • 황금(Scutellariae Radix)의 에타놀추출물과 플라보노이드 성분들의 독성평가

        정종평,구영,배기환 충남대학교 약학대학 의약품개발연구소 1995 藥學論文集 Vol.11 No.-

        Flavonoids from Scutellariae Radix possessed a dual function both as an anti-inflammatory agent and an enhancer of cel1ular activity in gingival fibroblast. The purpose of this study was to evaluate on the toxicity of ethanolic extract from the root of Scutellariae Radix Georgi and its flavonoids, Wogonin, Baicalein, and Baicalin were isolated and purified by the following method. The crude drug was extracted with ethyl acetate and the residue was dissolved in ethyl alcohol. The ethyl a1cohol soluble fraction was separated, concentrated, and men chromatogaphed on a silica gel column. The acute oral LD 50 in rats was determined for EtOH ex. of Scutellariae Radix and three compounds were evaluated with a single oral gavage at three graded dosage levels. The acute intravenous LD 50 was determined with a single intravenous injection via the jugular vein at three graded dosage levels. Groups of 5 male and 5 female rats, 6 week of age at the start of the study, were fed diets containing 3 graded dosage leve1s for 14 days. Groups of 5 male and 5 female hamster received 0.5㎖ of the test article at once in a day for 5 days to the buccal cheek pouch for two minutes each. The acute ora1 LD50 for EtOH ex. of Scutellariae Radix is 1430㎎/㎏, and for Wogonin 1320㎎/㎏, or Baicalein 1250㎎/㎏, for Baicalin 1330㎎/㎏. The acute intravenous toxicity of EtOH ex. of Scutellariae Radix and its extracts was found to be 27㎎/㎏ body weight No toxic effects were observed in rats fed up to 200㎎/㎏ of EtOH ex.of ScutelIariae Radix, Wogonin, Baicalein and Baicalin in the diet for 14 days. The acute Mucouse Membrane LD 50 in hamsters was found to be greater than 100㎎/㎏. These resu1ts suggested that EtOH ex. of Scutellariae Radix and its flavonoids are safe for oral care products using limited amount of extract.

      • 좌심실 장축 운동의 기능적 의의 (Ⅰ)

        박종훈,전은석,구대회 충남대학교 의과대학 지역사회의학연구소 1990 충남의대잡지 Vol.17 No.2

        To assess the functional significance of long axis movement which would reflect the fiber orientation of the ventricular muscle, we examined the mitral annulus motion and compared it with standard minor axis movement. M-mode echocardiogram were performed in 24 healthy individuals and 17 mitral valve replaced (MVR) patients with simultaneous EKG and phonocardiogram recordings. The results were as follows : 1. In the controls, the onset of shortening of long axis preceeds that of minor axis (phase difference : 20±3msec, mean± SEM) but in MVR patients this phase difference was reversed (-33±8msec). It means that the spherical shape changes which can be observed in normal controls during isovolumic contraction period were lost in MVR patients. 2. In MVR patients, the time intervals from A2 to the end of shortening of long axis were significantly delayed compared with normal controls (54±3 VS 90±8 msec, P<0.01). 3. In MVR patients, the delayed shortening of long axis was observed in amplitude as well as in time. In summary. we observed the regional nonuniformity between long axis and short axis in normal control and interpreted that it could play a role in modulating ventricular function which was lost in MVR patients.

      • 몇가지 植物中의 Vesicular-Arbuscular Mycorrhizae에 關하여(第三報)

        金鐘均,具順和 공주대학교 사범대학 과학교육연구소 1986 과학교육연구 Vol.18 No.1

        Mt. Kyeryong is in latitude 36°20'40" north and longitude 127°12'55" east, Mt. Chilgap is located in lati tude 36°24'35" north and longitude 126°57'30" east Two different areas in Chung-mam were examined by acid fuchsin staining for the presence of mycorrhizae. Fifty-seven species of herbaceous plants known as mycorrhizal species and non-mycorrhizal species were strictly divided by taxonomical way regardless of growth habits. 1. Out of 13 families 31 genus 45 species known as non-mycorrhizal species 6 families were proved to be non-mycorrhizal species and in some species of the other seven families VA-mycorrhiza was found. 2. In all 3 families 12 genus 12 species of two areas regarded as VA-mycor-rhizal species VA-mycorrhiza was observed. 3. But in 2 families 2 species known as non-mycorrhizal species the notable results were examined. Just in Commelina commmis in Chilgab and Galium verum var. asiatium in Gyeryong VA-mycorrhiza was found. According to the results of the experiment it seems that VA-mycorrhiza is affected by the taxonomical way more than by the environmental factors of habits.

      • 생활체육 참가가 중년여성의 자각적 스트레스에 미치는 영향

        강종구,김민희 全北大學校 敎育大學院 1997 敎育論叢 Vol.17 No.-

        The purpose of this study was to investigate the effects of participation in sport for all on middle-aged women were selected or subjects from Chonju, Kunsan and Iksan cities of chonbuk province, the psychological well-being index was used to investigate the stress level of the subjects. The contents of questionnaire were social performance and self-confidence, depression, sleeping disturbance and anxiety and general well-being and vitality. The subjects were divided into four groups. Group A was 44 women who had membership in sports club and participated 6 months periodically. Group B was 30 women who had did not have membership in sports club and had participated daily. Group C was 54 women who had never participated daily. Group D was 33 women who had participated one and two times a week. The results of the study were as follows. First, Group A was lower than Group C and D in stress factors of social performance and self-confidence, depression and sleeping disturbance and anxiety. Second, there was no difference in gener well-being and vitality in four groups. Third, Group A and B were lower than Group C and D in stress level. The conclusion four the above results is as follows : The women who participate in sports for all are lower than those who do not, in stress factor and stress level.

      • KCI등재후보

        심장수술 시 농축적혈구, 자가수혈기 혈액, 체외 순환기 혈액에서의 유리 혈색소 농도 비교

        송장호,구승우,백종화,정용보,이정순,최인철 대한마취과학회 2002 Korean Journal of Anesthesiology Vol.43 No.5

        Background: Intraoperative autotransfusion or residual blood in a cardiopulmonaty bypass (CPB) circuit has been used to reduce the need for an homologous blood transfutsion during cardiac surgery. However, it may contain some free hemoglobin released from damaged cells. The load of blood containing free hemoglobin may cause renal dysfunction. We measured the amount of free hemoglobin in backed blood, cell saver blood and CPB blood to evaluate what is the least hemolytic blood transfused in cardiac surgery. Methods: This study was performed in 20 patients undergoing cardiac surgery. In each patient, the banked blood, intraoperative salvaged blood with a cell saver and CPB residual blood were sampled at the end of the operation. The concentration of free hemoglobin, hemoglobin and platelet counts were measured in these blood samples and percent of hemolysis was calculated (%hemolysis = [free hemoglobin] / .free hemogloben + hemoglobin]) Results: In salvaged blood with a cell saver, CPB residual blood and banked blood, hemoglobin concentrations were 20.1 ± 2.7 g/dl, 8.0 ± 1.1 g/dl, and 22.2 ± 2.7 g/dl, respectively. Free hemoglobin concentrations were 336.6 ± 239.5 mg/dl, 49.2 ± 26.8 mg/dl, and 279.5 ± 167.5 mg/dl respectively. Platelet counts were 26.1 ± 22.2 × 10^3/mm^3, 116.8 ± 56.5 × 10^3/mm^3, and 94.9 ± 43.6 × 10^3/mm^3 respectively. % Hemolysis were 1.6 ± 1.1%, 0.6 ± 0.4%, and 1.2 ± 0.7% respectively. In the comparison between the salvaged blood with a cell saver and CPB residual blood, free hemoglobin concentration, % hemolysis, and platelet counts had positive correlations (r = 0.8, 0.7, and 0.6). Conclusions: In twenty cardiac surgeries, CPB residual blood had a lower free hemoglobin level than the other two blood groups. The platelet counts in CPB residual blood were higher than those in cell saver blood but did not differ from those in banked blood. Therefore, CPB residual blood was the least hemolytic blood among the three blood groups when a transfusion was performed in cardiac surgery. (Korean J Acesthesiol 2002; 43: 588~593)

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