Blocking of Cell Proliferation, Cytokines Production and Genes Expression Following Administration of Cordyceps Sinensis in the Bronchoalveolar Lavage Fluids Cells

Kuo, Yuh-Chi,Wu, Chia-Lin,Tasi, Wei-Jern,Wang, Jir-Yenn,Chang, Shi-Chung,Lin, Ching-Yuang,Shiao, Ming-Shi 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Yuh-Chi Kuo¹,Chia-Lin Wn¹,Wei-jern Tasi¹,Jir-Yenn Wang²,Shi-Chung Chang₃,Ching-Yung Lin⁴,and Ming-Shi Shiao⁴¹National Research Institute of Chinese Medicine, ²Department of Biology,Fu Jen University, ³Department of Chest, ⁴Department of Pediatrics, □De[art,amt of Medical Research and Education. Taipei Veterans General Hospital, Taipei, Taiwan Yuh-Chi Kuo¹,Chia-Lin Wn¹,Wei-jern Tasi¹,Jir-Yenn Wang²,Shi-Chung Chang²,Ching-Yung Lin³,and Ming-Shi Shiao⁴.¹National Research Institute of Chinese Medicine, ²Department of Biology,Fu Jen University, ³Department of Chest, ⁴Department of Pediatrics, □De[art,amt of Medical Research and Education. Taipei Veterans General Hospital, Taipei, Taiwan. Blocking of Cell Proliferation, Cytokines Production and Genes Expression Following Administration of Cordyceps sinensis in the Bronchoalveolar Lavage Fluids Cells. Proceedings of international Symposium on East-West Medicine, Seoul. 44-64, 1999.-Cordyceps sinensis is a major parasitic fungus on larva of Lepidoptera. It is one of the well know fungi used in traditional Chinese medicine for treatment asthma, lung and kideney diseases. Many fungi belonging to the genus Cordyceps have been demonstrated to produce natural products with various biological activities. Reported biological activities of genus cordyceps include:(a)inhibition of DNA and RNA synthesis;(b)enhancement of cell differentiation;(c)restruction of cytoskeleton;(d)inhibition of protein kinase activity;(e)antitumor activity on bladder, colon, lung carcinoma as well as fibroblastoma;(f)inhibition of the infection and revers transciptase activity of human immunodeficiency virus type 1; and(g) inhibition of methylation of nucleic acid. Thus, it is suggested that the extracts of Cordyceps sp. Contain antitumor, antiviral, and many other biological activities. In our previous studies, we proved that C. sinensis contains the antitumor and immunomodulatory agents. In the present studies, effects of C. sinensis on bronchoalveolar lavage fluids(BAL)cells were demonstrated. The crude methanolic extracts of C. sinensis were fractionated into 15 fractions by silica gel column chromatography. Effects of various fraction on BAL cells proliferation and lipopolysaccharide(LPS) stimulated interleukin-1β(IL-8),platelet activating factor(PAF), interleukin-6(IL-6), tumor necrosis factor-α(TNF-α),and interleukin-8(IL-8) production on BAL cells were determined. The result indicated that the CS-19-22 fraction suppressed BAL cells proliferation activated by LPS. The median inhibitory concentration(IC50) was 6㎍/ml. The CS-19-22 fraction also decreased IL-1β,PAF,IL-6, TNF-αand IL-8 production.The results of revers transcription-polymerase chain reaction(RT-PCR) demonstrated that the CS-19-22 fraction did not affect IL-1β,PAF,IL-6, TNF-α,and IL-8 mRNAs expression in BAL cells activated by LPS. By contrast, it inhibited the IL-10 mRNA expression but enhanced IFN-γand IL-12 mRNAs expression in activated BAL cells. Moreover, the CS-19-22 fraction blocked PAF-induced platelet aggregation. It is unlikely that cytotoxicity was involved. Because no cell deths were observable. We hypothesize that inhibitory mechanisms of CS-19-22 on BAL cells proliferation may be related to the impairments of gene expression and production of cytokines in BAL cells. The explanation of C. sinensis antiasthma function may involved the following(1)C.sinenesis contained immunomodulatory agents that stimulated IFN-γand IL-12 expression in TH1cells.(2)Enhanced secretion of IFN-γand IL-12 will inhibit the Tγcells immune responses Ex. Decreasing of IL-6 and IL-10 production.(3) The impairments of cytokines production in TH2 cells suppressed th BAL cells proliferation then more cytokines production Ex.IL-1β,PAF,IL-6,TNF-αand IL-8 in activated BAL cells were decreased. (4)The suppression of TH2cells immune responses will inhibit the growth and differentiation of B cells then IgE production will be decreased. (5)Reduced production of IgE will decreased asthma attack occurring in individuals. However, the results of the present study suggested that C. sinensis may also have acted to treat asthma in part by inhibiting BAL cells proliferation and cytokine gene expression and production. Future, experiments with treatment of animals with asthma with C. sinensis will be necessary to defined whether C. sinensis can reduce asthma injury. Plans are underway for the isolation of pure principal growth inhibitors and their mechanisms of action are subjected for further study.

Reversible Data Hiding Scheme for VQ Indices Based on Absolute Difference Trees

( Chin-chen Chang ),( Thai-son Nguyen ),( Chia-chen Lin ) 한국인터넷정보학회 2014 KSII Transactions on Internet and Information Syst Vol.8 No.7

Reversible data hiding is a technique for recovering original images without any distortion after secret data are extracted from the image. The technique continues to attract attention from many researchers. In this paper, we introduce a new reversible data hiding scheme based on the adjacent index differences of vector quantization (VQ) indices. The proposed scheme exploits the differences between two adjacent indices to embed secret data. Experimental results show that our scheme can achieve a lower compression rate than an earlier scheme by Yang and Lin. Our scheme`s average compression rate, 0.44 bpp, outperforms that of Yang and Lin`s scheme, which averages 0.53 bpp. Moreover, the embedding capacity of our scheme can rise to 1.45 bpi, which also is superior to that of Chang et al.`s scheme [35] (1.00 bpi)Yang and Lin`s scheme [27] (0.91 bpi) as well as Chang et al.`s scheme [26] (0.74 bpi).

ON HOLDER-MCCARTHY-TYPE INEQUALITIES WITH POWERS

Lin, Chia-Shiang,Cho, Yeol-Je Korean Mathematical Society 2002 대한수학회지 Vol.39 No.3

We extend the Holder-McCarthy inequality for a positive and an arbitrary operator, respectively. The powers of each inequality are given and the improved Reid's inequality by Halmos is generalized. We also give the bound of the Holder-McCarthy inequality by recursion.

VOCs absorption in rotating packed beds equipped with blade packings

Chia-Chang Lin,Yu-Chiao Lin,Kuo-Shin Chien 한국공업화학회 2009 Journal of Industrial and Engineering Chemistry Vol.15 No.6

This work investigated the volatile organic compounds (VOCs) removal efficiency of the RPB equipped with blade packings using absorption. Methanol, methyl ethyl ketone and methyl acetate were used as the model VOCs herein. The VOCs removal efficiency (E) was observed to increase with the rotational speed and the liquid flow rate but decrease with the gas flow rate. Moreover, methanol absorption provided the largest E values owing to that the solubility of methanol in water was the highest among three VOCs, implying that the centrifugal force induced from the RPB equipped with blade packings did not affect the thermodynamic property of VCOs. In addition, when the gas flow rate was 50 L/min, the highest E values for methanol, methyl ethyl ketone and methyl acetate absorption were 89.8, 77.6, and 68.9, respectively. Consequently, the RPB equipped with blade packings would be more applicable to the removal of more soluble VOCs from gaseous streams of huge flow rate.

Removal of ozone from air by absorption in a rotating packed bed

Chia-Chang Lin,Cheng-Yu Chao,Mei-Yun Liu 한국공업화학회 2010 Journal of Industrial and Engineering Chemistry Vol.16 No.1

This work investigated the feasibility of ozone (O3) absorption by H2O2 solution in a rotating packed bed (RPB). The overall volumetric gas-phase mass transfer coefficients (KGa) were determined as functions of pH of H2O2 solution, O3 concentration, H2O2 concentration, RPB speed, gas flowrate, liquid flow rate, and RPB type. The KGa values were highly dependent on the pH of H2O2 solution. Also, the KGa values increased with O3 concentration and H2O2 concentration. As expected, the RPB speed positively affected the KGa values for all RPBs. Furthermore, the obtained results indicated that the KGa values increased as the inner radius of the bed was increased and the outer radius of the bed was decreased. Moreover, the KGa values increased with an increasing liquid flow rate and an increasing gas flow rate for all RPBs. The dependences of KGa on the gas flow rate and the liquid flow rate indicated that the resistance to mass transfer in the gas-phase for O3 absorption was higher than that in the liquid phase in the RPB.

Analysis and Design of a PFC AC-DC Converter with Electrical Isolation

Lin, Chia-Ching,Yang, Lung-Sheng,Zheng, Ren-Jun The Korean Institute of Power Electronics 2014 JOURNAL OF POWER ELECTRONICS Vol.14 No.5

This study presents a single-phase power factor correction AC-DC converter that operates in discontinuous conduction mode. This converter uses the pulse-width modulation technique to achieve almost unity power factor and low total harmonic distortion of input current for universal input voltage $90V_{rms}$ to $264V_{rms}$) applications. The converter has a simple structure and electrical isolation. The magnetizing-inductor energy of the transformer can be recycled to the output without an additional third winding. The steady-state analysis of voltage gain and boundary operating conditions are discussed in detail. Finally, experimental results are shown to verify the performance of the proposed converter.

Analysis and Design of a PFC AC–DC Converter with Electrical Isolation

Chia-Ching Lin,Lung-Sheng Yang,Ren-Jun Zheng 전력전자학회 2014 JOURNAL OF POWER ELECTRONICS Vol.14 No.5

This study presents a single-phase power factor correction AC?DC converter that operates in discontinuous conduction mode. This converter uses the pulse-width modulation technique to achieve almost unity power factor and low total harmonic distortion of input current for universal input voltage (90 Vrms to 264 Vrms) applications. The converter has a simple structure and electrical isolation. The magnetizing-inductor energy of the transformer can be recycled to the output without an additional third winding. The steady-state analysis of voltage gain and boundary operating conditions are discussed in detail. Finally, experimental results are shown to verify the performance of the proposed converter.

RIGHT-HANDED SNEUTRINO CURVATON AND NON-GAUSSIANITY

LIN, CHIA-MIN,CHEUNG, KINGMAN World Scientific 2010 Modern Physics Letters A Vol.25 No.26

<P> In this paper, we explore the parameter space for a Right-Handed (RH) sneutrino curvaton that can generate large non-Gaussianity without assuming any particular inflation sector. The mass of the RH sneutrino is suggested from a discussion on the initial condition of the curvaton field. It is shown that a small Yukawa coupling is generally required for a successful RH sneutrino curvaton. However, the Yukawa coupling can be larger if we consider the braneworld scenario. Some general discussion about the spectral index in curvaton scenario is also provided. </P>

Developing a Virus-Binding Bacterium Expressing Mx Protein on the Bacterial Surface to Prevent Grouper Nervous Necrosis Virus Infection

( Chia-hua Lin ),( Jun-jie Chen ),( Chiu-min Cheng ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.8

Grouper nervous necrosis virus (GNNV) infection causes mass grouper mortality, leading to substantial economic loss in Taiwan. Traditional methods of controlling GNNV infections involve the challenge of controlling disinfectant doses; low doses are ineffective, whereas high doses may cause environmental damage. Identifying potential methods to safely control GNNV infection to prevent viral outbreaks is essential. We engineered a virus-binding bacterium expressing a myxovirus resistance (Mx) protein on its surface for GNNV removal from phosphate-buffered saline (PBS), thus increasing the survival of grouper fin (GF-1) cells. We fused the grouper Mx protein (which recognizes and binds to the coat protein of GNNV) to the C-terminus of outer membrane lipoprotein A (lpp-Mx) and to the N-terminus of a bacterial autotransporter adhesin (Mx-AIDA); these constructs were expressed on the surfaces of Escherichia coli BL21 (BL21/lpp-Mx and BL21/Mx-AIDA). We examined bacterial surface expression capacity and GNNV binding activity through enzyme-linked immunosorbent assay; we also evaluated the GNNV removal efficacy of the bacteria and viral cytotoxicity after bacterial adsorption treatment. Although both constructs were successfully expressed, only BL21/lpp-Mx exhibited GNNV binding activity; BL21/lpp-Mx cells removed GNNV and protected GF-1 cells from GNNV infection more efficiently. Moreover, salinity affected the GNNV removal efficacy of BL21/lpp-Mx. Thus, our GNNV-binding bacterium is an efficient microparticle for removing GNNV from 10‰ brackish water and for preventing GNNV infection in groupers.

Evaluation of a rotating packed bed equipped with blade packings for methanol and 1-butanol removal

Chia-Chang Lin,Yu-Chiao Lin,Shu-Ching Chen,Ling-Jung Hsu 한국공업화학회 2010 Journal of Industrial and Engineering Chemistry Vol.16 No.6

Absorption removal of methanol and 1-butanol from gaseous streams with water was investigated in the RPB equipped with blade packings. The removal efficiency (E) of methanol and 1-butanol was found to increase with the RPB speed and the liquid flow rate but decrease with the gas flow rate. Also, the overall volumetric gas-side mass transfer coefficient (KGa) for methanol and 1-butanol absorption was observed to increase with the RPB speed, the gas flow rate, and the liquid flow rate. According to the obtained dependence of KGa on the gas and liquid flow rates, the mass transfer in methanol and 1-butanol absorption was observed to be controlled primarily by the gas-side mass transfer. Furthermore, the height of a transfer unit (HTU) for methanol and 1-butanol absorption decreased with the RPB speed and the liquid flow rate but increased with the gas flow rate. The obtained results demonstrated that mass transfer efficiency of the RPB equipped with blade packing was comparable to that of a hollow fiber absorber. Consequently, the RPB equipped with blade packings has a great potential in the removal of alkanols from the exhausted gases. 2010 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.