유화처리 바이오디젤이 도포된 콘크리트의 침투깊이 판정

백철 ( Baek Cheol ),김태우 ( Kim Tae-woo ),이재진 ( Lee Jae-jin ),이동윤 ( Lee Dong-yun ),한민철 ( Han Min-cheol ),한천구 ( Han Cheon-goo ) 한국건축시공학회 2017 한국건축시공학회 학술발표대회 논문집 Vol.17 No.2

This study is to provide a evaluation method for the penetration depth of emulsified refined bio diesel(ERBD)applied to a surface of the concrete by using water absorption capability of the concrete. The concrete applied with ERBD was immersed at water for 1 min., 5min., and 10 min. and then was checked the brightness with elapse of time. Test results indicated that there was clear difference between ERBD part and non ERBD part in concrete specimen after measuring the brightness until 120min.

대장내시경검사의 전처치로서 항콜린제 사용에 관한 연구 : 전향적, 이중맹검법으로

박경남,한동수,이민호,최호순,박준용,손주현,이오영,함준수,전용철,송승찬,기춘석,윤병철,이종희 대한소화기내시경학회 1997 Clinical Endoscopy Vol.17 No.3

Background: Use of antispasmodic medication prior to colonoscopy is controversial but someone believes antispasmodic may improve visualization of colonic mucosa and ease colonoscope insertion. So, we designed a study to assess the effect of premedication with the antispasmodic, hyoscine-N-butyl bromide(Buscopan') on the performance of colonoscopy. Methods: This study was prospective, double blinded, randomized, controlled study, One hundred three consecutive patients were randomized to receive intravenous buscopan lml(n=52) or placebo(n=51) combined with our standard initial medication(me- peridine 50 mg and midazolam 2 mg). Insertion of colonoscopy was timed, and 100 mm visual analogue scales (VAS) were used for asscssing difficulty of procedure, colonic motility, frequency of positional change, frequency of external compression, difficulty of assistance and degree of discomfort experienced by the patients. Results: There were no significant differences of intubation time between buscopan group(mean time, 7.23 min., range 2~15) and placebo group(7.07 min., range 3-25), (p=0.83) and withdrawal time between buscopan group (6.46 min., range 2-22) and placebo group(6.76 min., range 2 25), (p=0.69). Also, there was no significant differences in intubation time between males and females(buscopan; males 7.00 min., females 7.60 min., p=0.34, placebo; males 7.0~5 min., females 7.08 min., p 0.44). The VAS scores checked by endoscopist(p=0.29), assistant(p=0.32) and patient (p=0.15) were not significantly different in both groups. There were no significant differences in intubation time, VAS scores nf endoscopist, assistant, and patients. Conclusion: Premedication with intravenous bu.opan has no advantage on colonoscopy procedure. Use of antispasmodic medication prior to colonoscopy was not considered as recommendable agent.

법랑모세포 분화와 성숙과정에서 OD314의 발현

박주철,안성민,김흥중,정문진,박민주,신인철,손호현 大韓齒科保存學會 2005 Restorative Dentistry & Endodontics Vol.30 No.5

법랑모세포는 법랑질을 형성하고 유지하는 세포로, 법랑질의 유기기질을 분비하고 법랑질 석회화 과정에도 관여한다. 치아 발생과정에서 법랑모세포의 분화는 순차적인 상피-간엽 상호작용에 의하여 조절되나, 분화나 성숙과정의 정확한 기전은 아직까지 잘 알려져 있지 않다. 최근에 상아모세포에서 처음 발견된 OD314가 치아 발생과정에서 상아질을 형성하는 상아모세포 뿐 아니라 법랑모세포에도 발현된다고 하였다. 이에 본 연구에서는 생쥐 하악 전치의 다양한 시기의 법랑모세포를 이용하여, 형태학적 분석과 in-situ hybridization에 의한 OD314 mRNA의 발현 그리고 OD314 항체를 이용한 면역조직화학적 분석을 통하여 OD314 유전자의 법랑 모세포 분화와 성숙과정에서의 역할을 연구하여 다음과 같은 결과를 얻었다. 1. 형태학적으로 법랑모세포는 분화 단계에 따라 분비 전단계 법랑모세포, 분비기 법랑모세포, 성숙기의 평탄끝 법랑모세포와 성숙기의 주름끝 법랑모세포로 구분되었다. 2. OD314 mRNA는 분비기의 법랑모세포에서부터 발현되기 시작하여 법랑모세포가 성숙해갈 수록 그 발현이 증가하였다. 3. OD314 단백질은 분비 전단계의 법랑모세포에서는 발현되지 않고, 분비기의 법랑모세포에서는 세포질에 전체적으로 발현되었다. 성숙기의 평탄끝 법랑모세포와 주름끝 법랑모세포에서는 세포의 근심과 원심끝단에 OD314 단백질이 강하게 발현되었다. 이상의 결과를 종합하여 OD314는 법랑모세포의 분화와 성숙과정에서 세포질 내부에서 특징적인 역할을 하는 것으로 사료된다. Ameloblasts are responsible for the formation and maintenance of enamel which is an epithelially derived protective covering for teeth. Ameloblast differentiation is controlled by sequential epithelial-mesenchymal interactions. However, little is known about the differentiation and maturation mechanisms. OD314 was firstly identifled from odontoblasts by subtraction between odontoblast/pulp cells and osteoblast/dental papilla cells, even though OD314 protein was also expressed in ameloblast during tooth formation. In this study, to better understand the biologcal function of OD314 during amelogenesis, we examined expression of the OD314 mRNA and protein in various stages of ameloblast differentiation using in-situ hybridization and immunohistochemistry. The results were as follows : 1. The ameloblast showed 4 main morphological and functional stages referred to as the presecretory, secretory, smooth-ended, and ruffle-ended. 2. ○D314 mRNA was expressed in secretory ameloblast and increased according to the maturation of the cells. 3. OD314 protein was not expressed in presecretory ameloblast but expressed in secretory ameloblast and maturative ameloblast. OD314 protein was distributed in entire cytoplasm of secretory ameloblast. However, OD314 was localized at the proxiamal and distal portion of the cytoplasm of smooth- ended and ruffle-ended ameloblast. These results suggest that ○D314 may play important roles in the ameloblast differentiation and maturation.

리더의 코칭리더십이 조직구성원의 창의적 행동에 미치는 영향: 그릿에 의해 조절된 전환학습의 매개효과 중심으로

신민철(Shin, Min-Cheol),오상진(Oh, Sang-Jin) 한국경영교육학회 2020 경영교육연구 Vol.35 No.2

[연구목적] 본 연구는 최근 급변하는 경영환경 속에서 지속가능경영을 발현하기 위해 창의적인 아이디어를 실행시킬 수 있는 리더의 코칭리더십을 이해하고, 코칭리더십과 창의적 행동 관계에서 그릿의 조절 효과와 전환학습의 조절된 매개효과를 실증하고, 시사점을 제공하고자 한다. [연구방법] 국내기업 종사자를 대상으로 설문 조사하였으며, 설문지는 온라인을 통한 질의응답 방식으로 688부를 배부하여 335부가 최종 분석에 사용되었다. 수집된 자료는 신뢰도와 타당성을 검증하였고, 가설검증은 부트스트랩 방식의 SPSS Process Macro 3.0을 사용하였다. [연구결과] 리더의 코칭리더십은 조직구성원의 전환학습과 창의적 행동에 유의한 영향을 미치는 것으로 나타났다. 조직구성원의 전환학습은 창의적 행동에 유의한 영향을 미쳤고, 코칭리더십과 창의적 행동 간의 관계를 매개했다. 조직구성원의 그릿과 전환학습은 리더의 코칭리더십과 그들의 창의적 행동 간의 관계에서 조절된 매개효과를 나타냈다. [연구의 시사점] 기업은 조직구성원의 창의적 행동을 증대시키기 위해서 리더의 코칭리더십 향상을 위한 다양한 투자와 노력이 필요하고, 조직구성원의 전환학습과 그릿을 높이기 위한 관심과 지원을 강화해야 하는 실무적 시사점을 제시하고 있다. [Purpose] This study was intended to identify the leader’s coaching leadership who can execute creative ideas to express sustainability management in the recent fast-changing business environment, demonstrate the moderating effect of GRIT and the moderated mediating effect of transformative learning in the relationship between coaching leadership and creative behavior. [Methodology] The survey was conducted on employees of domestic companies, and the questionnaire distributed 688 copies online and 335 copies were used for the final analysis. The data collected have been validated for reliability and validity, and the hypothesis testing uses SPSS Process Macro 3.0 by bootstrap. [Findings] The leader’s coaching leadership was shown to have a positive effect on the transformative learning and creative behavior of the organization members. The organization member’s transformative learning has had a positive impact on creative behavior, and has mediated the relationship between coaching leadership and creative behavior. The GRIT and transformative learning of organizational members have shown a moderated mediating effect in the relationship between the coaching leadership and their creative behavior. [Implications] This research presents practical implications that various investments and efforts are needed to improve the coaching leadership in order to increase the creative behavior of the members of the organization and to strengthen interest and support to enhance the transformative learning and GRIT.

비만학생들의 운동후 회복시 초과 산소소비량 및 지속시간에 관한 연구

박철호,권민호,고봉민,박은경,우상헌 東亞大學校附設스포츠科學硏究所 1999 스포츠科學硏究論文集 Vol.17 No.-

The purpose of this study was to examine the effect of two different intensity of continuous exercise on magnitude of excess post-exercise oxygen consumption(EPOC), energy consumption following a 20min treadmill exercise. The subjects of this study were eight super-obesity in middle school students who were above 13 years old. subjects participated in two different exercise intensity to measure respiratory gas, blood lactate concentration and eardrum temperature. The results of this study were as follows: 1. The Energy consumption of the high intensity (80% VO₂ max) exercise group (264.68 35.60cal) was greater than that of the low intensity (50%) exercise group(172.01 20.39 Cal)(p<.001). 2. The EPOC of the high intensity exercise group (22.36+ 6.82Cal ) was greater than that of the low intensity exercise group(15.39+5.27 Cal)(p<.001). 3. The EPOC duration of th high intensity exercise group(28.36=3.56min) was longer than that of the low intensity exercise group(17.23+1.25min)(p<.001) 4. The blood lactate concentration, respiratory exchange ratio and temperature of the high intensity exercise group were greater than those of the low intensity exercise group(p<.05,p<.01). EPOC and duration were increase with 80%VO₂max, 80% VO₂max as intensity exercise got higher this also showed that it had something to do with lactate concentration in blood and increase of body temperature in the conclusion. We considered that there was a difference in using methods of energy, as there was a similar difference in RER. We though this following with oxygen consumption after exercise, we could clarify that high intensity exercise was better than lower intensity exercise in sametime for those who are obese especially adolescents to maintain their weights on the aspect of effect.

천연 갈변 억제제 개발을 위한 양파,사과 및 감귤 과피 추출물의 항산화 및 갈변 저해 효과

장민선 ( Min Sun Chang ),안세진 ( Se Jin An ),정문철 ( Moon Cheol Jeong ),김동만 ( Dong Man Kim ),김건희 ( Gun Hee Kim ) 한국식품영양학회 2011 韓國食品營養學會誌 Vol.24 No.3

This study was carried out to investigate antioxidative properties of various extracts and antibrowning effects of extracts in apple slices were investigated by ΔE value and PPO relative activity. Apples were cut into 1.5 ㎝ thickness and they were dipped in 1% extract solutions(OW: water extracts of onion, OE: 80% EtOH extracts of onion, AW: water extracts of apple, AE: 80% EtOH extracts of apple, MW: water extracts of mandarin orange peel, ME: 80% EtOH extracts of mandarin orange peel) for 1 min. OW showed higher than the other treatments for total phenolic contents(94.35 ㎎/g), PPO inhibition(74.00%). And the highest DPPH free radical scavenging activity(40.27%) measured in ME. ΔE value of apple slices dipped in MW was 2.37 whereas ΔE value of apple slices dipped in AW was 12.12. These results suggest that onion and mandarin orange peel extracts should be a potential source for controlling browning during storage of apple slices.

입원한 정신분열증 환자의 우울과 불안 : Longitudinal Study According to the Level of GAF 전반적 기능평가 척도 수준에 따른 종단적 연구

박민철,장광철 大韓神經精神醫學會 1992 신경정신의학 Vol.31 No.3

Forty schizophrenics who had been admitted to Wonkwang University Neuropsychiatric Hospital from August 1990 to February 1991 were rated for depression and anxiety levels three times longitudinally, when their GAF scores reached 30 and below, 31 and over and 40 and below, and 41 and over. At each time, the depression and anxiety levels were assessed by using self rating scales (SCL-90-R, SDS, and STAI-I) and objective scales(BPRS, HDS, and HAS). The results were as follows : 1) For the depression, the schizophrenics showed significantly low depression scores on the self-rating scale(SCL-90-R : DEP) and all of the objective rating scales(BPRS : DEP, HDS) according to their GAF scores reached the above levels(p〈0.01). 2) For the anxiety, the schizophrenics showed significantly low anxiety scores on the self-rating scale(SCL-90-R : ANX) and all of the objective rating scales(BPRS : ANX, HAS) according to their GAF scores reached the above levels(p〈0.01). The above results suggest that as the functioning level improved depression and anxiety also improved.

미더덕의 생리활성이 향상된 아임계수 추출물의 제조

조민지(Min-Ji Jo),한지경(Ji-Kyoung Han),성수창(Su-Chang Sung),이승철(Seung-Cheol Lee) 한국식품영양과학회 2018 한국식품영양과학회지 Vol.47 No.11

미더덕의 이용 다변화를 위하여 아임계수 추출물을 제조하고 생리활성을 평가하였다. 미더덕은 동결건조 분말 상태로 10, 30, 60분 동안 다양한 온도(50, 100, 200, 300℃)에서 추출되었다. 아임계수 조건은 ACE 저해 활성, 항산화 활성, AChE 저해 활성과 같은 중요한 생리학적 특성을 상당히 증가시켰다. 200℃, 60분 추출물에서 가장 높은 ACE 저해활성을 나타냈으나 더 높은 온도 조건인 300℃에서는 급격히 감소하였다. DPPH 라디칼 소거능과 ABTS라디칼 소거능은 300℃, 30분 추출물에서 공통적으로 가장 높은 값을 보였고, AChE 저해 활성 또한 같은 경향을 나타냈다. 이러한 결과는 아임계수 추출이 미더덕의 생리활성을 증가시키는 유용한 가공방법이 될 수 있음을 시사한다. For diverse application of Styela clava (Korean name: miduduck) as a food material, subcritical water (SCW) extract was prepared and its physiological activity was evaluated. To accomplish this, S. clava powder (0.1 g) was placed in a stainless vessel containing 10 mL of distilled water, after which SCW extraction was carried out at 50, 100, 200, and 300℃ for 10, 30, and 60 min. SCW treatment significantly increased important physiological properties of the extract such as angiotensin converting enzyme (ACE) inhibitory activity, antioxidant activity, and acetylcholinesterase (AChE) inhibitory activity. The highest ACE inhibitory activity was found in samples subjected to SCW extraction at 200℃ for 60 min; however, the activity decreased at higher temperature (300℃). The SCW extract of S. clava prepared at 300℃ for 30 min showed the highest antioxidant activity [1,1-diphenyl-2-picrylhydrazyl and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activities] and AChE inhibitory activity. These results indicate that SCW extraction might be a useful method for increasing the physiological activity of S. clava.

흰쥐의 중추성 Serotonin 고갈이 강제수영 중 부동자세 시간, 불안 및 수동적 회피 학습에 미치는 효과

박제민,김종백,강철중 大韓神經精神醫學會 1996 신경정신의학 Vol.35 No.3

흰쥐에서 p-chlorphenylalanine(PCPA)로 인한 강제수영 중 부동자세 시간의 단축이 불안이나 수동적 회피 학습 능력의 변화와 관련된 것인가를 검증하고자 하였다. 흰쥐에게 PCPA 300mg/kg 혹은 생리식염수를 하루 한 번 연속 3회 복강내 주사하였다. 마지막 주사 24시간 후에 공간에서의 활동성(ACT0), 높은 십자 통로 실험에서 개방 통로에 머무는 시간(ANX0), 왕복 회피상자에서 통과 지연 시간(STL0) 등을 측정하고 0.5mA 전기자극을 3초간 주어 회피 반응을 학습시켰다. 그리고 15분간 강제수영시켰다. 다음 날 같은 방법으로 활동성(ACT1), 불안(ANX1), 수동적 회피 반응 학습 정도(STL1), 5분간의 강제수영 중 총 부동 자세 시간 등을 측정하였다. 마지막 약물 혹은 대조액 투여 7일 후 수동적 회피 학습 유지 정도(STL7)를 다시 측정하였다. 결과는 아래와 같다. 1) 전체 대상군에서 ACT1은 ACT0보다 감소하였다. PCPA는 개방공간에서의 활동성에 영향을 주지 않았다. 2) PCPA 군은 높은 십자 통로 실험에서 ANX0와 ANX1 둘 다 대조군 보다 유의하게 길었다. 3) STL1은 PCPA군과 대조군 모두 STL0 보다 유의하게 지연되었다. PCPA는 STL0 이나 STL1에 영향을 주지 않았다. 그러나 대조군은 수동적 회피 학습 7일 후에도 통과 지연 시간이 유의하게 연장되어 있는데 반하여 PCPA군은 STL7이 STL0과 유의한 차이가 없었다. 4) 강제수영 중 부동자세 시간은 PCPA군이 대조군보다 유의하게 짧았다. 이상의 결과에서 PCPA 투여로 인한 강제수영 중 부동자세 시간의 단축은 PCPA가 비특이적 신체 활동을 증가시키거나 수동적 회피 반응 습득을 방해함으로써 일어나는 현상이 아니며, 진정 작용없이 탐색 행동을 증가시키거나, 스트레스 하에서의 행동 억제 현상을 차단함으로써 강제수영 중 부동자세 시간을 단축시킨다고 추론한다. 아울러 PCPA가 기억 고정 과정을 방해할 가능성이 있음을 제안한다. Purpose : The test if p-chlorophenylalanine(PCPA) induced decrease of immobility time during forced swimming test in rats is an epiphenomenon related with PCPA-induced changes in anxiety level or learning ability. Methods : 22 male rats(Sprague Dawley, weighting 180-280gm) were randomly assigned to PCPA or control group and treated with 300mg/kg of PCPA or saline i.p. at -72, -48, and -24 hrs. Number of segment(ACT0) entered with 4 limbs on the open field(L100×W100×H40cm, 25 segments), time(ANX0, sec) spent on the open arm(L110×W10cm, 50cm from the ground) in the elevated plus maze, and step-through latency(STL0, cut-off time : 300sec) in the shuttle box(L76.2×W34.3×H50.8cm, 7.5w electric bulb in the light chamber, 0.5mA for 3 sec in the dark chamber) were measured consequently at 24hrs after the last injection of PCPA or saline. After 20min of rest, animals were forced to swim for 15 min(cylinder : φ18×H40cm ; water : H15cm, 25℃). On the next day, the second trial of open field test(ACT1), elevated plus maze test(ANX1), passive avoidance learning test(STL1), and measurement of total immobility time(IT, sec) during the 5min of forced swim were done in the same manner as the first trial except that electrical shock was not given to the animals in the passive avoidance learning test and duration of forced swimming was shortened from 15min to 5min. To evaluate longterm effect of PCPA on maintenance of memory, measurement of step-through latency was repeated 7 days after the first trial(STL7). Result: 1) ACT1 was significantly decreased in the pooled sample, but the effect of PCPA on the general motor activity was not significant. 2) PCPA significantly prolonged the time spent on the open arms in the elevated plus maze test. 3) Comparing to STL0, STL1 was significantly prolonged in both groups. PCPA did not affect STL0 or STL1. STL7 was significantly prolonged in the control group, but not in the PCPA group. 4) PCPA shortened IT significantly. Conclusion : PCPA-induced decrease of immobility time during the forced swimming test in rats seems to be related with disinhibition of stress-related behavioral suppression. PCPA does not affect acquisition and short-term maintenance of passive avoidance learning, but necessity of further studies about its effect on long-term maintenance on memory is raised.

Effects of Dilute Acid Pretreatment on Enzyme Adsorption and surface Morphology of Liriodendron tulipifera

Byeong Cheol Min,Bon Wook Koo,Ki Seob Gwak,Hwan Myeong Yeo,Joon Weon Choi,In Gyu Choi 한국목재공학회 2011 목재공학 Vol.39 No.2

In this study, dilute acid pretreatment of Liriodendron tulipifera was performed for enzymatic hydrolysis. As the pretreatment temperature was increased, enzymatic hydrolysis and enzyme adsorption yield also increased. The highest enzymatic hydrolysis yield was 57% (gig) and enzyme adsorption was 44% (gig). Enzymatic hydrolysis yield was determined with weight loss of pretreated biomass by enzyme, and enzyme adsorption was a percentage of enzyme weight attaching on pretreated biomass compared with input enzyme weight. When L. tulipifera was pretreated with 1% sulfuric acid at 160℃ for 5 min., hemi-cellulose was significantly removed in pretreatment, but the lignin contents were constant. Other changes in surface morphology were detected on biomass pretreated at 160℃ by a field emission scanning electron microscope (FESEM). A large number of spherical shapes known as lignin droplets were observed over the entire biomass surface after pretreatment. Hemicellulose removal and morphological changes improved enzyme accessibility to cellulose by increasing cellulose exposure to enzyme. It is thus evidence that enzyme adsorption is a significant factor to understand pretreatment effectiveness.