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Antitumor Activity of Histone Deacetylase Inhibitor Trichostatin A in Osteosarcoma Cells
Cheng, Dong-Dong,Yang, Qing-Cheng,Zhang, Zhi-Chang,Yang, Cui-Xia,Liu, Yi-Wen Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4
Background: Histone deacetylase (HDAC) inhibitors have been reported to induce cell growth arrest, apoptosis and differentiation of tumor cells. The present study aimed to examine the effects of trichostatin A (TSA), one such inhibitor, on the cell cycle, apoptosis and invasiveness of osteosarcoma cells. Methods: MG-63 cells were treated with TSA at various concentrations. Then, cell growth and apoptosis were determined by 3-(4, 5-dimethyl-2-thiazolyl)-2H-tetrazolium bromide (MTT) and TUNEL assays, respectively; cell cycling was assessed by flow cytometry; invasion assays were performed with the transwell Boyden Chamber system. Results: MTT assays revealed that TSA significantly inhibited the growth of MG-63 cells in a concentration and time dependent manner. TSA treated cells demonstrated morphological changes indicative of apoptosis and TUNEL assays revealed increased apoptosis of MG-63 cells after TSA treatment. Flow cytometry showed that TSA arrested the cell cycle in G1/G2 phase and annexin V positive apoptotic cells increased markedly. In addition, the invasiveness of MG-63 cells was inhibited by TSA in a concentration dependent manner. Conclusion: Our findings demonstrate that TSA inhibits the proliferation, induces apoptosis and inhibits invasiveness of osteosarcoma cells in vitro. HDAC inhibitors may thus have promise to become new therapeutic agents against osteosarcoma.
Cheng-Cheng Lin,Zhi-Ying Xu,Bi-Han Wang,Wenyue Zhuang,Jinghui Sun,He Li,JianGuang Chen,Chunmei Wang 한국식품영양과학회 2021 Journal of medicinal food Vol.24 No.8
Schisandra chinensis (S. chinensis) is one of the core drugs used for relieving cough and asthma in traditional Chinese medicine. However, there are few basic studies on the treatment of respiratory diseases with S. chinensis in modern pharmacology, and the material basis and mechanism of its antiasthmatic effect are still unclear. Lignans are the main active components of S. chinensis. The aim of this study was to observe the relaxation effect of S. chinensis lignans (SCL) on the tracheal smooth muscle of rats by in vitro tracheal perfusion experiments, and to explore the mechanism by preincubation with L-type calcium channel blocker verapamil, four potassium channel blockers glibenclamide, tetraethylamine, 4-aminopyridine and barium chloride (BaCl2), β-adrenoceptor blocker propranolol, nitric oxide synthase inhibitor Nω-nitro-L-arginine methyl ester (L-NAME), and the cyclooxygenase inhibitor indomethacin, respectively. The results showed that SCL (0.25–1.75 mg/mL) reduced the contraction of isolated tracheal smooth muscle induced by acetylcholine, the preincubation with verapamil and glibenclamide could attenuate the relaxation effect, whereas propranolol, 4-aminopyridine, BaCl2, tetraethylamine, L-NAME, and indomethacin had no such effect. These results suggest that SCL has a significant relaxation effect on the isolated tracheal smooth muscle of rats, and the mechanism may be related to the inhibition of extracellular calcium influx and intracellular calcium release from the sarcoplasmic reticulum, as well as the activation of ATP-sensitive potassium channels. These findings may provide a pharmacological basis for the traditional use of S. chinensis to treat asthma.
Zhi Hui Liu,Wen Jun Xu,Sheng Yu Li,Cheng Zhi Long,Jia Ru Lin 한국전자통신연구원 2016 ETRI Journal Vol.38 No.5
In this paper, cognitive radio and simultaneous wireless information and power transfer (SWIPT) are effectively combined to design a spectrum-efficient and energy-efficient transmission paradigm. Specifically, a novel SWIPT-based primary-secondary cooperation model is proposed to increase the transmission rate of energy/spectrum constrained users. In the proposed model, a multi-antenna secondary user conducts simultaneous energy harvesting and information forwarding by means of power splitting (PS), and tries to maximize its own transmission rate under the premise of successfully assisting the data delivery of the primary user. After the problem formulation, joint power splitting and beamforming optimization algorithms for decode-and-forward and amplify-and-forward modes are presented, in which we obtain the optimal PS factor and beamforming vectors using a golden search method and dual methods. Simulation results show that the proposed SWIPTbased primary-secondary cooperation schemes can obtain a much higher level of performance than that of non-SWIPT cooperation and non-cooperation schemes.
Zhi-Qiang Song,Ju-E Cheng,Fei-Xue Cheng,De-Yong Zhang,Yong Liu 한국식물병리학회 2017 Plant Pathology Journal Vol.33 No.2
Tylenchulus semipenetrans is an important and widespreadplant-parasitic nematode of citrus worldwideand can cause citrus slow decline disease leading tosignificant reduction in tree growth and yield. Rapidand accurate detection of T. semipenetrans in soil isimportant for the disease forecasting and management. In this study, a loop-mediated isothermal amplification(LAMP) assay was developed to detect T. semipenetrans using DNA extracted from soil. A setof five primers was designed from the internal transcribedspacer region (ITS1) of rDNA, and was highlyspecific to T. semipenetrans. The LAMP reaction wasperformed at 63°C for 60 min. The LAMP productwas visualized directly in one reaction tube by addingSYBR Green I. The detection limit of the LAMP assaywas 10–2 J2/0.5 g of soil, which was 10 times moresensitive than conventional PCR (10–1 J2/0.5 g of soil). Examination of 24 field soil samples revealed that theLAMP assay was applicable to a range of soils infestednaturally with T. semipenetrans, and the total assaytime was less than 2.5 h. These results indicated thatthe developed LAMP assay is a simple, rapid, sensitive,specific and accurate technique for detection of T. semipenetrans in field soil, and contributes to the effectivemanagement of citrus slow decline disease.
Zhi-Lin Cheng,Yan-xiang Li,Zan Liu 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.55 No.-
Graphene oxide (GO)/silicalite-1 composites (GS) with hierarchical porous structure were fabricated by grafting GO onto acid-treated silicalite-1 zeolite. The structure and physical properties of the GS composites were determined by using a series of characterization techniques. The removal of rhodamine B (RB) from aqueous solution was conducted on the GS composites. Effects of experimental conditions on the adsorption were studied. The adsorption behaviors of the GS composites were well described by Langmuir isotherm and shown to follow a pseudo-second-order reaction model. The maximum adsorption capacity of the GS composites for RB was shown to 56.55 mg/g.
CLV1 Interacts with UFO in Modulation of Gynoecium Development in Arabidopsis thaliana
Zhi-Peng Cheng,Zhong-Nan Yang,Sen Zhang 한국식물학회 2013 Journal of Plant Biology Vol.56 No.1
The gynoecium is the female reproductive structurein flowering plants. It has been known that the CLAVATA1(CLV1) gene regulates the floral meristem maintenance andfloral organ number in Arabidopsis thaliana. However,whether CLV1 plays roles in gynoecium development remainsuncharacterized. In this study, we showed that CLV1genetically interacts with Unusual Floral Organs (UFO) toregulate the development of Arabidopsis gynoecium. Throughforward genetic screen, we identified a clv1 allele, clv1-9,showing abnormal gynoecium development. Some epidermalcells on the growing inner surfaces of the clv1-9 gynoeciumfailed to fuse after contacting with each other, suggesting thatCLV1 is essential for proper postgenital fusion. Furthermore,the ufo clv1-9 double mutant exhibited an expanded uppergynoecium, narrowed lower part of the ovary, alteredvascular patterning and abaxial outward proliferation of thetransmitting tract tissues. The transcription level of the ETTgene, which is required for gynoecium structure and patternformation, was down-regulated in the ufo clv1-9 doublemutant genoecia. Taken together, our results indicate thatCLV1 functions together with UFO to regulate gynoeciumdevelopment likely through modulating the expression ofETT.