A Comparative Study on the Importance and Frequency of Practice of Clinical Teaching Behaviors in Taiwan

Hui-Chen Hsu 한국간호과학회 2005 한국간호과학회 학술대회 Vol.2005 No.10

Ceramide Synthase 6 Mediates Triple-Negative Breast Cancer Response to Chemotherapy Through RhoA- and EGFR-Mediated Signaling Pathways

Hui Chen,Bin He,Feng Ke 한국유방암학회 2022 Journal of breast cancer Vol.25 No.6

Purpose: Limited treatment options and lack of treatment sensitivity biomarkers make the clinical management of triple-negative breast cancer (TNBC) challenging. Ceramide synthase 6 (CERS6) generates ceramides, which are key intermediates in sphingolipid biosynthesis and play important roles in cancer progression and resistance. Methods: CERS6 was analyzed to determine its potential as a treatment sensitivity biomarker. CERS6 levels were determined in patients with breast cancer, and the roles and downstream signaling of CERS6 were analyzed using cellular and biochemical assays. Results: Analysis of CERS6 expression in 195 patients with TNBC and their clinical response to chemotherapy revealed that individuals with CERS6 overexpression experienced significantly inferior responses to chemotherapy than those without CERS6 overexpression. Functional analysis demonstrated that although CERS6 overexpression did not affect TNBC cell growth and migration, it conferred chemoresistance. CERS6 inhibition significantly reduced growth, migration, and survival by suppressing the RhoA- and EGFR-mediated signaling pathways. Compared to control cells, CERS6-depleted cells were consistently less viable at different concentrations of chemotherapeutic agents. Conclusion: Our study is the first to demonstrate that CERS6 may serve as a treatment sensitivity biomarker in patients with TNBC in response to chemotherapy. In addition, our findings suggested that CERS6 may be a therapeutic target for TNBC treatment.

Exploring the Chronic Kidney Disease Patient Uses the Network Problem

Hui-Chen Chiu 한국간호과학회 2009 한국간호과학회 학술대회 Vol.2009 No.10

The most ideal interval between blastocyst biopsy and vitrification applied in preimplantation genetic screening (PGS)

( Hui-ying Low ),( Hsiu-hui Chen ),( Chun-chia Huang ),( Tsung-hsien Lee ),( Chung-i Chen ),( Lii-sheng Huang ),( Maw-sheng Lee ) 대한산부인과학회 2016 대한산부인과학회 학술대회 Vol.102 No.-

Study Question: To evaluate the most ideal interval between blastocyst biopsy and vitrification in preimplantation genetic screening (PGS). Study Design, Size, Duration: This is a retrospective study and total 224 patients underwent the PGS from 2012 Dec. to 2015 Mar. All of patients underwent blastocyst vitrification after biopsy and 1~2 euploid blastocyst for transfer after warming. The primary outcome measures were the implantation and pregnancy rates per PGS-frozen embryo transfer cycle. Materials, Setting, Methods: The blastocyst grading including grade 4, 5 and 6 (according to Gardner grading system) on day 5 or day 6 were selected for trophectoderm biopsy. All blastocyst underwent vitrification immediately (interval: 0.5 hour) or 1 to 7 hours after biopsy. At the time of vitrification the grade of blastocyst expansion was also recorded. All patients were divided into two groups according to the grade of expanded (Group1: ≤1/2 expansion (n=41), Group2: ≥3/4 expansion (n=183)). Furthermore, combined two factors including the interval and morphology of blastocyst after biopsy, all patients were further divided into interval 1 (<3 hours and ≤1/2 expansion) and interval 2 (≥3 hours and ≥3/4 expansion). The morphologically best euploid blastocyst(s) (1~2 embryos) was/were selected first for transfer on the next cycle. Main Results: Assessment morphology of blastocyst after biopsy in different interval, at 0.5 hour after biopsy, 100% blastocyst was non-expansion; at 1 hour after biopsy, only 17% blastocyst was 3/4 expansion or all-expansion; at 3 hours after biopsy, 86% blastocyst was 3/4 expansion or all-expansion and after 5.5 hours, 100% blastocyst was all-expansion or hatching. All blastocysts were survival (100%, 359/359) after warming. The mean of embryo transfer number between all groups were no significantly difference. The implantation rate in Group2 (63.4%) was significantly higher than that in Group1 (46.9%, p=0.014). The pregnancy rates in Group4 (73.8%) was sig-nificantly higher than that in Group1 (51.2%, p=0.004). The implantation and pregnancy rates in the group of embryo ≥3/4 expansion combined with ≥3 hours after biopsy (63.6%, 178/280; 73.8%, 127/172) were significantly higher than that in the group of ≤1/2 expansion with <3 hour (45.6, 26/57; 50.0%, 18/36; p=0.0113 and p=0.0056, respectively). Conclusion: The most ideal interval between biopsy and vitrification was least 3 hours and ≥3/4 expansion of blastocyst after biopsy could improve the implantation and pregnancy rates for PGS.

Protective effect of lncRNA CRNDE on myocardial cell apoptosis in heart failure by regulating HMGB1 cytoplasm translocation through PARP-1

Hui Chen,Jinming Liu,Bin Wang,Yongjun Li 대한약학회 2020 Archives of Pharmacal Research Vol.43 No.12

Long non-coding RNAs (lncRNAs) are boundup with the regulation of various diseases. Here, we probedinto the effect of lncRNA colorectal neoplasia differentiallyexpressed (CRNDE) on heart failure (HF). The pathologicalalterations and cell apoptosis of heart tissues were observedby hematoxylin–eosin and TUNEL staining. The viabilityor apoptosis of mouse myocardial cells HL-1 was tested byXTT or flow cytometry. The interaction between lncRNACRNDE and poly-ADP-ribose polymerase 1 (PARP-1) wasverified by RNA immunoprecipitation and RNA pull-down. The stability of the PARP-1 protein and the acetylation levelof high mobility group box-1 (HMGB1) were determined bycycloheximide-chase and immunoprecipitation, respectively. LncRNA CRNDE expression was decreased in HF micetissues and doxorubicin (Dox)-treated HL-1 cells, whereasPARP-1 and HMGB1 were increased. The overexpressionof lncRNA CRNDE restrained HL-1 cell apoptosis inducedby Dox. Moreover, the interaction between CRNDE andPARP-1 was corroborated, CRNDE negatively regulatedPARP-1 expression, and the overexpression of CRNDEreduced PARP-1 protein stability. In HL-1 cells, PARP-1positively regulated the acetylation level and cytoplasmtranslocation of HMGB1. CRNDE restrained Dox-inducedapoptosis in mouse myocardial cells via the PARP-1/HMGB1 pathway.

Association between ABCB1 Polymorphisms and Antidepressant Treatment Response in Taiwanese Major Depressive Patients

Hui Hua Chang,Chen-Hsi Chou,Yen Kuang Yang,I Hui Lee,Po See Chen 대한정신약물학회 2015 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.13 No.3

Objective: The multidrug resistance 1 (ABCB1, MDR1) gene, encoding P-glycoprotein, is extensively distributed and expressed in various tissues, such as a blood-brain barrier transporter. P-glycoprotein plays an important role in controlling the passage of substances between the blood and brain. The current study aimed to investigate possible associations of functional ABCB1 polymorphisms (C3435T, G2677T and C1236T) with response to antidepressant treatment and serum cortisol levels in Taiwanese patients with major depressive disorder (MDD). Methods: We recruited 112 MDD patients who were randomized to fluoxetine (n=58, mean dose: 21.4±4.5 mg/day) or venlafaxine (n=54, 80.2±34.7 mg/day) treatment for 6 weeks. The 21-item Hamilton Depression Rating Scale (HDRS) was administered initially and biweekly after treatment, and cortisol levels were assessed initially and after 6-week antidepressant treatment. Results: The initial HDRS scores and the HDRS scores after six weeks of antidepressant treatment were not significantly different among the different genotypes in each polymorphism of ABCB1. The percentage changes of HDRS scores over time were significantly different in the polymorphisms of ABCB1 G2677T (p=0.002). MDD patients with the G/G genotype of ABCB1 G2677T had a worse antidepressant treatment response. However, the polymorphisms of ABCB1 genotypes were not significantly associated with cortisol levels before and after antidepressant treatment in MDD patients. Conclusion: The results suggested that the variants of ABCB1 may influence the short-term antidepressant response in MDD patients. Further details of the underlying mechanisms of ABCB1 in antidepressant treatment remain to be clarified.

Pretreated quercetin protects gerbil hippocampal CA1 pyramidal neurons from transient cerebral ischemic injury by increasing the expression of antioxidant enzymes

Chen, Bai Hui,Park, Joon Ha,Ahn, Ji Hyeon,Cho, Jeong Hwi,Kim, In Hye,Lee, Jae Chul,Won, Moo-Ho,Lee, Choong-Hyun,Hwang, In Koo,Kim, Jong-Dai,Kang, Il Jun,Cho, Jun Hwi,Shin, Bich Na,Kim, Yang Hee,Lee, Y Medknow PublicationsMedia Pvt Ltd 2017 Neural regeneration research Vol.12 No.2

<P>Quercetin (QE; 3,5,7,3′,4′-pentahydroxyflavone), a well-known flavonoid, has been shown to prevent against neurodegenerative disorders and ischemic insults. However, few studies are reported regarding the neuroprotective mechanisms of QE after ischemic insults. Therefore, in this study, we investigated the effects of QE on ischemic injury and the expression of antioxidant enzymes in the hippocampal CA1 region of gerbils subjected to 5 minutes of transient cerebral ischemia. QE was pre-treated once daily for 15 days before ischemia. Pretreatment with QE protected hippocampal CA1 pyramidal neurons from ischemic injury, which was confirmed by neuronal nuclear antigen immunohistochemistry and Fluoro-Jade B histofluorescence staining. In addition, pretreatment with QE significantly increased the expression levels of endogenous antioxidant enzymes Cu/Zn superoxide dismutase, Mn superoxide dismutase, catalase and glutathione peroxidase in the hippocampal CA1 pyramidal neurons of animals with ischemic injury. These findings demonstrate that pretreated QE displayed strong neuroprotective effects against transient cerebral ischemia by increasing the expression of antioxidant enzymes.</P>

Dioxygen Activation bya Non-Heme Iron(II) Complex: Theoretical Study toward UnderstandingFerric–Superoxo Complexes

Chen, Hui,Cho, Kyung-Bin,Lai, Wenzhen,Nam, Wonwoo,Shaik, Sason American Chemical Society 2012 Journal of chemical theory and computation Vol.8 No.3

<P>We present a systematic study using density functional theory (DFT) and coupled cluster (CCSD(T)) computations with an aim of characterizing a non-heme ferric-superoxo complex [(TMC)Fe(O-2)(2+) (TMC = 1,4,8,11-tetramethyl-1,4,8,11-tetraazacyclotetradecane) that was proposed to perform allylic C-H activation of cyclohexene (Lee, Y.-M. et al. J. Am. Chem, Soc. 2010, 132, 10668). As such, we investigated a series of iron-O-2 species without and with a sixth ligand bound to the iron ion in different O-2 coordination modes (end-on and side-on) and different spin states. Most of the iron-O-2 complexes were found to be iron(III)-superoxo species, Fe(III)(O-2(-)), with high-spin (S = 5/2) or intermediate-spin (S = 3/2) ferric centers coupled ferromagnetically or antiferromagnetically to the superoxide anion radical. One iron(IV)-peroxo state, Fe(IV)(O-2(2-)), was also examined. The preference for ferromagnetic or antiferromagnetic coupling modes between the superoxo and ferric radicals was found to depend on the FeOO angle, where a side-on tilt favors ferromagnetic coupling whereas the end-on tilt favors antiferromagnetic states. Experimental findings, e.g., the effects of solvent, spin state, and redox potential of non-heme Fe(II) complexes on O-2 activation, were corroborated in this work. Solvent effects were found to disfavor O-2 binding, relative to the unbound ferrous ion and O-2. The potential H-abstraction reactivity of the iron(III)-superoxo species was considered in light of the recently proposed exchange-enhanced reactivity principle (Shaik, S.; Chen, H.; Janardanan, D. Nat. Chem. 2011, 3, 19). It is concluded that localization and/or decoupling of an unpaired electron in the d-block of high-spin Fe(III) center in the S = 2 and 3 ferric-superoxo complexes during H abstractions enhances exchange stabilization and may be the root cause of the observed reactivity of [(TMC)Fe(O-2)](2+).</P>

A Case Study on City Park’s Stormwater Management of Sponge City in China

Chen, Xiao Hui(진효훼),Pei, Fang Ming(배방명),Kim, Shin Won(김신원) 한국디자인문화학회 2018 한국디자인문화학회지 Vol.24 No.4

최근 중국에서 유행하는 경관생태 개념인 스펀지 도시(해면도시)는 점진적으로 중시되고 있다. 본 연구는 스펀지 도시의 이론을 파악해 중국 도시의 선진적인 우수관리 시스템 건설을 위한 기초 개념을 제공하고, 건설 방법을 제시하는 한편 이론적 결과를 바탕으로 하여 실천에 참고가 되는 자료를 제시하는데 연구의 목적을 둔다. 본 논문은 스펀지 도시 이론을 바탕으로 하여 건설된 예시를 연구의 대상으로 정하였다. 연구 방법은 스펀지 도시 이론의 우수관리를 분석하며, 우수관리의 구역적 특성, 조경 디자인의 생태성, 서비스의 다양성 등 3가지 특징을 나타낸다. 그 특징에 의해 지정된 3가지 부족한 면모는 천진 Qiaoyuan(橋園) 습지공원, 첸안 Sanlihe(三里河) 생태행랑과 하얼빈 Qunli(群力) 우수공원을 비교분석하였다. 예시 분석을 통하여 우수관리의 장점과 단점을 발견하여 스펀지 도시의 건설을 위한 지침을 제시하고자 하였다. 연구 결과, 해면체로서의 도시공원은 주민에게 휴식과 오락 장소를 제공함과 동시에 동식물에게 서식지를 제공하기도 하였다. 도시 홍수의 감소 및 수자원 재활용의 측면에서 도시 전체를 여전히 적용하지 않았는데, 지방의 환경은 발전과 개선의 측면에서 적극적으로 발휘되었다. 앞선 연구의 내용으로 보아 이론과 실천 경험의 부족함으로 인해 건설된 해면체로서의 도시공원은 수질 정화 및 지하수 보급의 측면에서 피할 수 없는 결점이 존재한다는 것을 확인하였다. 문화와 역사를 내포한 스펀지 도시를 건설하는 것은 인간의 과거와 미래 문명 발전을 지지하기도 한다. 본 연구는 미래 스펀지 도시의 발전 및 관련 연구에 가치 있는 자료로 활용되기를 기대한다. China has been aware of the popularity of an ecological concept, the Sponge City. This is a study of the theory for the Sponge City which points out an excellent direction of the stormwater management for Chinese cities. Meanwhile, the research of the construction of the Sponge City would provide references for theory and practice studies. This research takes examples of Sponge City as objects for study. The research methodology is infestation and analysis for stormwater management under the Sponge City theory, to summarize the three features of regional characteristics, ecology in landscape design, and diversity of services. By comparing these three features in Tianjin Qiaoyuan Wetland Park, Qian’an Sanlihe Ecological Corridor, and Harbin Qunli Stormwater Park, this paper discovered the advantages and disadvantages of stormwater management, provding inspirational suggestions for the construction of the Sponge City. Comprehensively, the Sponge City can provide residents not only a place to take a rest and entertain but an environment for plants and animals to live as an urban park. Although the problems of city stormwater management and the replenishment of water resources still exist, the Sponge City plays a vital role in the development and improvement of the zonule environment. Due to the lack of theoretical and practical experience, the established Sponge City park has certain defects in water purification and groundwater recharge. As a carrier of culture and history, the construction of the Sponge City also bears the development of human civilization in the past and the future. As a textual material, this research may be used as a reference for the future development of the Sponge City.

ACOS5 is Required for Primexine Formation and Exine Pattern Formation During Microsporogenesis in Arabidopsis

Hui-hui Xie,Lin Chen,Fa-qing Xu,Wan-sheng Guo,Shui Wang,Zhong-Nan Yang,Sen Zhang 한국식물학회 2017 Journal of Plant Biology Vol.60 No.4

Pollen exine, mainly composed of sporopollenin,plays important roles during microspore development. It hasbeen reported that Acyl-CoA Synthetase5 (ACOS5) is requiredfor sporopollenin biosynthesis in Arabidopsis. Here we showthat ACOS5 is essential for primexine formation duringArabidopsis microspore development. Through genetic screen,we identified a point mutation of ACOS5 allele, acos5-2,showing abnormal microspore development. Its microsporeswere degenerated and aborted after released from the tetrads. Transmission electron microscopy showed that primexineformation was reduced in acos5-2 mutant as compared tothat of the wild-type. Consequently, sporopollenin wasaggregated and randomly deposited on the microspores. Insitu hybridization indicated that the key regulators of tapetumdevelopment, DYT1 and TDF1, are required for the expressionof ACOS5 in tapetum. Furthermore, the GUS reporter showedthat the 593-bp promoter sequence was sufficient for theexpression of ACOS5 in the anther. Our data provide evidencethat ACOS5 is required for primexine formation andsporopollenin deposition during microspore development.