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Bacillus megaterium Strain XTBG34 Promotes Plant Growth by Producing 2-Pentylfuran
Changsong Zou,Zhifang Li,Diqiu Yu 한국미생물학회 2010 The journal of microbiology Vol.48 No.4
Several chemical changes in soil are associated with plant growth-promoting rhizobacteria. An endosporeforming bacterium, strain XTBG34, was isolated from a Xishuangbanna Tropical Botanical Garden soil sample and identified as Bacillus megaterium. The strain’s volatiles had remarkable plant growth promotion activity in Arabidopsis thaliana plants; after 15 days treatment, the fresh weight of plants inoculated with XTBG34 was almost 2-fold compared with those inoculated with DH5α. Head space volatile compounds produced by XTBG34, trapped with headspace solid phase microextraction and identified by gas chromatography–mass spectrometry, included aldehydes, alkanes, ketones and aroma components. Of the 11 compounds assayed for plant growth promotion activity in divided Petri plates, only 2-pentylfuran increased plant growth. We have therefore identified a new plant growth promotion volatile of B. megaterium XTBG34, which deserves further study in the mechanisms of interaction between plant growth-promoting rhizobacteria and plants.
Analysis of the Cold-Responsive Transcriptome in the Mature Pollen of Arabidopsis
Zou Changsong,Yu Diqiu 한국식물학회 2010 Journal of Plant Biology Vol.53 No.6
Mature pollen is very sensitive to cold stress in chilling-sensitive plants. To understand the genetic regulation of tolerance to cold stress, we analyzed the transcript expression profile in mature pollen of Arabidopsis using Affymetrix GeneChips containing ∼24,000 genes. Expression of 2,127 genes was cold-regulated, of which 697 genes were upregulated and 1,430 genes were downregulated. Further analysis showed that a large number of signal transduction components were significantly affected by cold treatment, indicating extensive changes in the gene regulatory networks of mature pollen. Many coldresponsive genes encode transcription factors, suggesting a multitude of transcriptional cascades. A number of genes important for the biosynthesis or signaling of plant hormones, such as abscisic acid, auxin, and jasmonate,were regulated by cold stress, which is of potential importance in coordinating cold tolerance with pollen growth and development. In addition, 159 mature pollenspecific genes that might be involved in pollen viability were also cold-regulated. Expression of the cold-responsive transcripts identified by microarray analysis was confirmed by quantitative real-time PCR. Our study provides an overall picture of the cold-responsive transcriptome in Arabidopsis pollen and is valuable for understanding gene regulation in response to cold stress and the molecular mechanisms of cold tolerance in mature pollen.
Yu, Changsong,Jia, Gang,Deng, Qiuhong,Zhao, Hua,Chen, Xiaoling,Liu, Guangmang,Wang, Kangning Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.5
Glucagon-like peptide-2 (GLP-2) is important for intestinal barrier function and regulation of tight junction (TJ) proteins, but the intracellular mechanisms of action remain undefined. The purpose of this research was to determine the protective effect of GLP-2 mediated TJ and transepithelial electrical resistance (TER) in lipopolysaccharide (LPS) stressed IPEC-J2 cells and to test the hypothesis that GLP-2 regulate TJ and TER through the phosphatidylinositol 3-kinase (PI3K)-protein kinase B (Akt)-mammalian target of rapamycin (mTOR) signaling pathway in IPEC-J2 cells. Wortmannin and LY294002 are specific inhibitors of PI3K. The results showed that $100{\mu}g/mL$ LPS stress decreased TER and TJ proteins occludin, claudin-1 and zonula occludens protein 1 (ZO-1) mRNA, proteins expressions (p<0.01) respectively. GLP-2 (100 nmol/L) promote TER and TJ proteins occludin, claudin-1, and zo-1 mRNA, proteins expressions in LPS stressed and normal IPEC-J2 cells (p<0.01) respectively. In normal cells, both wortmannin and LY294002, PI3K inhibitors, prevented the mRNA and protein expressions of Akt and mTOR increase induced by GLP-2 (p<0.01) following with the significant decreasing of occludin, claudin-1, ZO-1 mRNA and proteins expressions and TER (p<0.01). In conclusion, these results indicated that GLP-2 can promote TJ's expression and TER in LPS stressed and normal IPEC-J2 cells and GLP-2 could regulate TJ and TER through the PI3K/Akt/mTOR pathway.
이창송 ( Changsong Lee ),이호경 ( Ho Kyung Lee ) 한국공업화학회 2015 공업화학전망 Vol.18 No.5
데이터의 축적량 및 처리 기술의 발전으로 오늘날 빅데이터 기술은 다양한 산업에 걸쳐 활발히 활용되고 있다. 가격 경쟁력 이외에 품질 및 수율의 차별화가 요구되는 제조업의 경우에도 예외가 아니다. 여기에서는 화학 산업의 제조 공정에 대한 빅데이터 적용 사례로서 불량 원인 분석을 통한 품질개선, 생산성 향상, 예측모델 기반 최적화, 그리고 에너지 절감 사례를 다루고자 한다.
Yu, Changsong,Jia, Gang,Jiang, Yi,Deng, Qiuhong,Chen, Zhengli,Xu, Zhiwen,Chen, Xiaolin,Wang, Kangning Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.5
The glucagon-like peptide 2 (GLP-2) that is expressed in intestine epithelial cells of mammals, is important for intestinal barrier function and regulation of tight junction (TJ) proteins. However, there is little known about the intracellular mechanisms of GLP-2 in the regulation of TJ proteins in piglets' intestinal epithelial cells. The purpose of this study is to test the hypothesis that GLP-2 regulates the expressions of TJ proteins in the mitogen-activated protein kinase (MAPK) signaling pathway in piglets' intestinal epithelial cells. The jejunal tissues were cultured in a Dulbecco's modified Eagle's medium/high glucose medium containing supplemental 0 to 100 nmol/L GLP-2. At 72 h after the treatment with the appropriate concentrations of GLP-2, the mRNA and protein expressions of zonula occludens-1 (ZO-1), occludin and claudin-1 were increased (p<0.05). U0126, an MAPK kinase inhibitor, prevented the mRNA and protein expressions of ZO-1, occludin, claudin-1 increase induced by GLP-2 (p<0.05). In conclusion, these results indicated that GLP-2 could improve the expression of TJ proteins in weaned pigs' jejunal epithelium, and the underlying mechanism may due to the MAPK signaling pathway.
Synthesis of CdS Nanocrystals with Different Shapes via a Colloidal Method
Jie Bai,Changsong Liu,Jinzhong Niu,Hongzhe Wang,Shasha Xu,Huaibin Shen,Lin Song Li 대한화학회 2014 Bulletin of the Korean Chemical Society Vol.35 No.2
Size- and shape-controlled monodisperse wurtzite structured CdS nanorods have been successfully synthesized using a facile solution-based colloidal method. Depending on the control of injection/growth temperatures and the variation of Cd-to-S molar ratios, the morphology of the CdS nanocrystals (NCs) can be adjusted into bullet-like, rod-like, and dot-like shapes. X-ray diffraction (XRD), transition electron microscopy (TEM), and absorption spectroscopy were used to characterize the structure, morphology, and optical properties of assynthesized CdS NCs. It was found that uniform CdS nanorods could be successfully synthesized when the injection and growth temperatures were very high (> 360 °C). The aspect ratios of different shaped (bullet-like or rod-like) CdS NCs could be controlled by simply adjusting the molar ratios between Cd and S.
Phylogenetic Analysis on the Bacteria Producing Non-Volatile Fungistatic Substances
ZhiFang Li,ChangSong Zou,YueQiu He,MingHe Mo,KeQin Zhang 한국미생물학회 2008 The journal of microbiology Vol.46 No.3
This study characterized the soil bacteria producing non-volatile fungistatic substances. Among the 2,100 colonies of soil bacteria randomly isolated from seven agricultural soil samples, 518 isolates (24.67% of total) showed fungistatic activity toward nematophagous fungi Paecilomyces lilacinus and Trichoderma viride by producing non-volatile substances. A phylogenetic analysis based on amplified ribosomal DNA restriction analysis (ARDRA) and 16S rDNA sequence placed the 518 bacteria in three groups of the domain Bacteria: Actinomycetales, Bacillales, and Gammaproteobacteria. Three genera, Arthrobacter, Bacillus, and Pseudomonas, were the most frequently encountered groups.
Guozhen Hu,Changsong Chen,Duan Shanxu 전력전자학회 2013 JOURNAL OF POWER ELECTRONICS Vol.13 No.2
The use of LCL filters in pulse width modulation voltage source converters is a standard solution for providing proper attenuation of high-order grid-current harmonics. However, these filters can cause the undesired effect of resonance. This paper proposes an active damping strategy with harmonics compensation. It can alleviate the harmonics around the resonance frequency caused by the LCL filters. The proposed strategy is attractive since it is simple, does not depend on grid parameters and does not increase the number of sensors. Simulation and experimental results verify the effectiveness of the proposed active damping strategy.