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Cymbidium mild mosaic virus의 분리동정
Chang M. U.,Doi Y.,Yora K. 한국응용곤충학회 1978 한국식물보호학회지 Vol.17 No.3
Cymbidium의 mild mosaic 병주로부터 각종 초본식물에 즙액접종되는 소구형 바이러스를 분리하여 다음과 같은 결과를 얻었다. 1. 본 바이러스를 즙액접종하였을 때 C. amaranticlor, C. quinoa, Cymbidium spp., Dianthus caryophyllus는 전신감염, C.ficifolium, Gomphrean globosa는 국부감염되었다. 2. 본 바이러스는 Myzus persicae로 전반되지 않고, 영양번식기관에 의하여 전반되었다. 3. 조즙액중의 불활성화한계는 내열성이 $90^{\circ}C$ (10분)이고 내희석성이 $10^{-6}$, 내보존성이 60일 $(20^{\circ}C)$이였다. 4. 본 바이러스는 C. amaranticolor병엽을 동결후, chlorform으로 청등하여 분획원심분리와 sucrose density gradient 원심분리법으로 순화하였다. 5. 순화시료의 자외선흡수곡선은 최고 261nm, 최저 243nm이고 260/280=1.72, 최고/최저=1.26의 핵단백에 의한 흡수곡선을 나타냈다. 침강계수는 $S_{20,w}=126$의 수치가 얻어졌다. 6. 本 바이러스의 항혈청은 침강반응혼합법에 의해 2,025배의 역가를 나타냈고, 한천내확산법에 의한 시험에서 CarMV와 혈청학적관계가 있는 것으로 나타났다. 7. 本 바이러스의 형태는 직경 약 28nm의 소구형(다면체) 입자이고, empty particles도 소수 관찰되었다. 5. 본 바이러스에 감염된 C. amaranticolor, C. ficifolium, Cymbidium spp.의 병엽초박절편을 전자현미경으로 관찰하였든 바, 각종 세포의 세포질, 액포 및 도관내에 소구형 입자가 산재 또는 집괴의 소재양식으로 확인되었다. 9. 이상의 결과를 종합해서 본 바이러스를 Cymbidium mild mosaic virus로 명명한다. A virus named Cymbidium mild mosaic virus(Cy MMV), was mechanically transmitted to Chenopodium amaranticolor from the leaves of Cymbidium with mild mosaic symptoms. The virus was cultured in C. amaranticolor, in which it produced local chlorotic and ring spots, followed by systemic vein clearing with distortion. CyMMV infected 7 out of 35 species of plants. In C. amaranticolor juice infectivity was lost by heating at $90^{\circ}C$ for 10 miuntes, and by aging at$20^{\circ}C$ for 60 days, and by diluting at $10^{-6}$ when bioassayed on C. amaranticolor. CyMMV was not transmitted by Myzus persicae. The virus was purified after clarification of homogenized C. amaranticolor leaf tissues with chloroform, by differential centrifugation followed by sucrose density gradient centrifugation. Electron microscopic examination of purified preparation showed spherical particles of 28nm in diameter. The UV absorption spectrum of purified preparation was typical of u nucleoprotein (max. at 261nm. min. at 243nm), and showed 260/280=1.72 and max/min=1.26. The value of the sedimentation coefficient of the virus was S20.w=126. In gel-diffusion tests, CyMMV antiserum reacted with CarMV, but not with any of four other viruses (BBWV, CRSV, CMV, TBRV) having similar particles and properties in vitro. In ultra-thin sections of CyMMV infected tissues, a large number of virus particles were found in the cytoplasm of mesophyll cells and in xylem vessels.
A Novel Fault Location Scheme on Korean Electric Railway System Using the 9-Conductor Representation
Changmu Lee,Hansang Lee,Dong-Hee Yoon,Hanmin Lee,Jiyoung Song,Gilsoo Jang,Byungmoon Han 대한전기학회 2010 Journal of Electrical Engineering & Technology Vol.5 No.2
This paper presents a novel fault location scheme on Korean AC electric railway systems. On AC railway system, because of long distance, 40[㎞] or above, between two railway substations, a fault location technique is very important. Since the fault current flows through the catenary system, it must be modeled exactly to analyze the fault current magnitude and fault location. In this paper, suggesting the novel scheme of fault location, a 9-conductor modeling technique including boost wires and impedance bonds is introduced based on the characteristics of Korean AC electric railway. After obtaining a 9-conductor modeling, the railway system is constructed for computer simulation by using PSCAD/EMTDC. By case studies, we can verify superiority of a new fault location scheme and propose a powerful model for fault analysis on electric railway systems.
이장무(Changmu Lee),이한민(Hanmin Lee),김주락(Jurak Kim),김길동(Gildong Kim) 대한전기학회 2006 대한전기학회 학술대회 논문집 Vol.2006 No.7
The more feeding distance of substation is long and travel vehicle increases in feeding section, power supply reaches the limits. And if it exceeds the limit, it causes a serious trouble for train operation. Therefore, it is designed that the number of train increases constantly until 2030. So consistent power supply according to the increasement of vehicle in power system at present Seoul-Pusan high speed railway, regular operation in case of extended feeding occurring accidents in substation, and the least headway scheduled at relevant section and the effects installing compensation facility in case of impossible extended feeding is examined.
이장무(Lee Changmu),김주락(Kim Joorak),이한민(Lee Hanmin),오세찬(Oh Sechan),김길동(Kim Gildong) 한국철도학회 2006 한국철도학회 학술발표대회논문집 Vol.- No.-
In case of accidents at the line of Seoul-Pusan high speed railway, high speed train should be operated with making a detour by using existing Seoul-Pusan railroad. For Dae-jeon~Dae-Gu section which has fragile structures, following points are investigated. In operating existing train, train running time is analyzed by the case of only high speed train being in service and the case of high speed train being in service on condition that existing train is operated. Also the possibility about operation on present train headway and train headway that is applicable to operation and vocational problems would be examined.
Eight Previously Unreported Species of Macrofungi from Korea
( Jin Sung Lee ),( Changmu Kim ),( Sunyoung Choi ),( Hyang Burm Lee ) 한국균학회 2017 韓國菌學會誌 Vol.45 No.4
This study evaluated macrofungal specimens collected from the Gangwon, Jeonnam, and Chungnam provinces of Korea in 2015~2016. All specimens were identified at the species level using a combination of morphological characteristics and rDNA sequence data. Among them, eight macrofungal species, Coprinopsis romagnesiana, Daldinia loculata, Gymnopus biformis, Gymnopus montagnei, Gymnopus sepiiconicus, Hebeloma danicum, Pholiota limonella, and Russula brevipes, are new records for macromycota in Korea.