Rat의 복강내에 투여한 5-fluorouracil-polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

강대영,송규상,최정목,노승무,정경수,오정연,김진향,양준묵,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

For more effective intraperitoneal chemotherapy, the authers made 5-fluorouracil-polyglycolic acid composite disks(FU-PGA disks) with 5-fluorouracil and polyglycolic acid, a biodegradable polymer. We inserted the FU-PGA disk into the peritoneal cavites of Sprague-Dawley male rats, the control and three experimental groups; one FU-PGA disk insert group(300mg/kg), two FU-PGA disk insert group(600mg/kg), and three FU-PGA disk insert group(900mg/kg). The control group received a similar number of PGA disk inserts. A pharmacokinetic study was performed to measure the 5-fluorouracil concentration in the peritoneal fluid, blood, and tissues(liver, kidney and heart) at 24 hours, 72hours and 168 hours after insertion of the FU-PGA disk. Light and electron microscopic studies were done. The results were as follows: 1) The number of white blood cells and platelets decreased after FU-PGA insertion. The degree was proportional to the duration and amounts inserted. The change in the number of red blood cells varied slightly. 2) Light microscopically, slight changes were noted at 168 hours in the 3 disk insert group (900mg/kg). Mild fatty change and hepatocyte degeneration around the central veins of the liver were noted, with vacuolar degeneration in the proximal convoluted tubules of the kidney. 3) Electron microscopically,the liver showed focal increases of lipid droplets in the hepatocytes, and irregular nuclear membrane with focal nucleolar segregation of the fibrillar and granular elements. Also the double membranous structure of the hepatocytes and Kupffer cells was widened with bleb formation. The kidney showed wide separation of the nuclear double membrane in the proximal convoluted tubular cells and mesangial cells, with vacuolation and myelin figure formation in the mitochondria of the proximal convoluted tubular cells, at 72 hours and 168 hours in the 2 to 3 FU-PGA insert groups. The heart showed focal loss of cristae,vacuolation and myelin figure formation in the mitochondria of the 2 to 3 FU-PGA insert groups. In conclusion, despite the large amont of FU-PGA inserted, the histopathological changes in the liver, kidney and heart were slight, and consonant with the very low amount of 5-fluorouracil concentrations detected in the liver, kidney and heart. The above results suggest that the FU-PGA composite can serve as a new device for releasing drugs in a controlled manner and easily targeted to intraperitoneal organs. This device can improve the efficacy of intraperitoneal chemotherapy for gastric cancer.

Predictive Risk Assessment Using Cooperation Concept for Collision Avoidance of Side Crash in Autonomous Lane Change Systems

Hyunsoo Lee,Chang Mook Kang,Wonhee Kim,Woo Young Choi,Chung Choo Chung 제어로봇시스템학회 2017 제어로봇시스템학회 국제학술대회 논문집 Vol.2017 No.10

We present an innovative approach to risk assessment using cooperation concept for collision avoidance of side crash for autonomous lane change system. The cooperation concept is proposed to check an available space in the target lane for lane change in a short period of the future. Before moving to the target lane, the ego vehicle needs to check whether the object vehicle is cooperative or not for the lane change. The object vehicles are indexed as cooperative driver (CD) or non-cooperative driver (NCD) through the conditions based on the relative longitudinal acceleration and the collision free time. If the object vehicle is CD, the ego vehicle moves toward the target lane within the original lane for the preparation of the lane change. The ego vehicle should keep its lateral position within the original lane until the situation for the lane change becomes safe. As soon as the risk assessment becomes safe, the lane change is performed. Therefore, lane change can be started in advance compared to the conventional methods. The proposed method was validated via computational simulation and experimental results.

Hexestrol의 投與가 不垂體剔出 흰쥐의 子宮에 미치는 影響

田暢淇,金榮默 충남대학교 대학원 1975 論文集 Vol.5 No.-

In order to find out the functional relationship between ovary and uterus not related to other endocrine glands in albino rats, weight of uterus, height of endometrial epithelium, width of muscular layer and histological change were investigated in hypophysectmized rats after ad mininistration of hexestrol (6.0 ㎎/rat) during ranging 1-56 days, and the results obtained are as follows. 1. The weight of uterus was decreased gradually in hypophysectomized group without treatment of hexestrol but was increased in hexestrol treatment group until 14 days and decreased thereafter. The significance between both group was highly recognized. 2. The height of endometrial epithelium, width of circular and longitudinal muscle layer of uterus showed high significance as compared with control group. Those value in hypophysectmized group was decreased as the time passed and in hexestrol treatment group showed the peak at 14th day. 3. The tissue of uterus was reacted sensitively with hexestrol and the change of hyperlasia was recognized clearly but hypophysectomized group showed the change of degeneration. 4. It was showed that uterus was only controlled by ovary but other endocrine glands were not directly affected on uterus.

鹽化알루미늄 重合體와 黃酸알루미늄의 凝集效果에 對한 比較 硏究

李基昌,徐廷穆 명지대학교 1976 明大論文集 Vol.9 No.-

현재 우리 나라에서 일차 정수제로 사용되고 있는 황산알루미늄(황산반토)의 결점과 문제점을 해결하기 위하여, 새로운 정수제의 개발이 요청되고 있다. 본 연구에서는 염화알루미늄 중합체(polyaluminumchloride, P.A.C)와 황산알루미늄에 의한 혼탁한 원수 중에서의 제탁효과를 정성 및 정량적으로 비교 실험하였다. 실험 결과는 P.A.C가 황산알루미늄에 비하여 floc의 흡착 및 활성도가 비교적 크고, 강함을 보여 주었으며, 원수의 탁도와 수온의 고저에 관계 없이, 좋은 제탁효과를 나타내었다. P.A.C는 사용시에 알칼리도(alkalinity)의 감소가 적게 나타나기 때문에, 알칼리 보조제의 사용량도 크게 절감할 수 있음을 보여 주었다. 또, P.A.C는 적정 사용량의 허용폭이 커서, 작업상 안정도를 높일 수 있었다. This is to examine the coagulating effects of polyaluminumchloride in water, a kind of inorganic high polymer according to the experimental method. Besides, it is meaningful to compare the coagulating effects of aluminumsulphate utilizing as a coagulants in our country, and then to examine the efficient usage of polyaluminumchloride. The results of the above are as follows: 1. The characteristics of floc showed that the absorption activity is stronger than alum. 2. It expressed that the coagulating effects are good in spite of changing the turbidity and temperature of water. 3. The required quantities of alkaline agents are decreased, because alkalinities neary are not changing. 4. One of the results said that, as the permissive amount of dosage is large, there is safety in the treatment of water. As the above consequences, disclosed that only to utilize the polyaluminumchloride as coagulant is more effective than to uitlize aluminumsulphate, and then very promising.

만성 승모판 패쇄부전증 환자에서의 안지오텐신 전환효소 억제제의 장기 투여 효과

김대희,이명묵,이해영,조현재,박승정,서재빈,서정원,양한모,윤창환,조상호,이준희,김용진,김명아,손대원,오병희,박영배 대한순환기학회 2004 Korean Circulation Journal Vol.34 No.2

파라미터 해석을 통한 차량 성능 예측 기법 연구

김기창(Kim, Ki-Chang),김찬묵(Kim, Chan-Mook),김진택(Kim, Jin-Taek) 한국소음진동공학회 2010 한국소음진동공학회 논문집 Vol.20 No.11

With the development of the auto industry, the automobile manufacturers demand to shorten development period and reduce the cost. Compared with the traditional method, applying the virtual prototype is more economical. This paper presents a method for parameters sensitivity analysis and optimizing the performance of vehicle noise and vibration. The existing design processes were repeatedly analyzed with a focus on vehicle performance to decide the design parameters of dimension, thickness, mounting type of body and chassis systems in the vehicle development period. This paper describes the prediction technique of vehicle performance using L18 orthogonal array layout, quality deviation analysis and parameter sensitivity analysis for robust design. This paper analyzed the performance correlation equation through the frequency and sensitivity database according to a design factor change. The new concept is that the performance prediction is possible without repeated activities of test and analysis. This paper described the parameter analysis applications such as bush dynamic stiffness and bush void direction of rear suspension. Design engineer could efficiently decide the design variable using parameter analysis database in early design stage. These improvements can reduce man hour and test development period as well as to achieve stable NVH performance.

6-OHDA가 흰쥐 등쪽솔기핵내 도파민성 신경세포와 별아교세포에 미치는 영향

홍은석,남성안,조승묵,강태천,원무호,최창토 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.4

5, 7-DHT가 흰쥐 등쪽솔기핵내 신경아교세포에 미치는 영향

채제명(Je Myung Chae),조승묵(Seung Mook Jo),남성안(Seong Ahn Nam),윤상선(Sang Seon Yoon),고병문(Byung Moon Ko),최창도(Chang Do Choi),최월봉(Wol Bong Choi) 대한해부학회 1998 Anatomy & Cell Biology Vol.31 No.1

신경독성물질인 5, 7-dihydroxytryptamine (5, 7-DHT)이 중추신경계통내 serotonin 신경세포에 미치는 영향과 이에 따른 신경아교세포들의 반응을 형태학적으로 규명하고자 본 연구를 시행하였다. 흰쥐의 가쪽뇌실에 5, 7-DHT(200μg)를 투여한 후면역세포화학적 염색을 시행한 후 광학 및 전자현미경하에서 등쪽솔기핵내 serotonin 신경세포에 초래되는 퇴행성변화와 주위 신경아교세포들의 반응양상 등을 경시적으로 (1, 3, 5, 10 및 20일) 관찰하였던 바 아래와 같은 결과를 얻었다. 흰쥐 등쪽솔기핵내 5, 7-DHT의 세포독성에 반응을 보인 신경아교세포로는 미세아교세포와 별아교세포였으며, 이들 세포 들의 반응양상은 매우 특징적이었으며, 상당한 차이를 보였으며, 전반적으로 미세아교세포가 별아교세포에 비해 빠른 변화소견을 보였다. 즉, 미세아교세포의 경우 실험 3일군 및 5일군에서 이미 그 수의 증가와 함께 활발한 포식작용을 보였던 반면, 별아교세포는 후기 실험군인 10일군 및 20일군에 이르러서야 세포질의 비대 및 수적 증식을 통하여 손상된 신경조직을 보상할 뿐 아니라 밀집된 미세아교세포들을 분산시키는 소견이 관찰되었다. 그러나 희소돌기아교세포는 본 연구의 전 실험군을 통하여 특별한 변화소견이 관찰되지 않았다. 이상의 결과로 부터 5, 7-DHT가 흰쥐 등쪽솔기핵내 신경세포에 강한 세포독성을 유발하며, 이에 따른 주위 신경아교세 포들 특히 미세아교세포와 별아교세포는 시간경과에 따라 매우 다양한 반응양상을 보인다는 사실을 알게 되었다 This study was designed to clarify the cytotoxic effects of 5, 7-dihydroxytryptamine (5, 7-DHT) on the serotonergic neurons in the dorsal raphe nucleus, and to investigate the glial reaction during the neurodegenerative changes by light and electron microscopy. Adult male rats (Sprague-Dawley strain) weighing from 250 g to 350 g each were used as experimental animal. 5, 7-DHT(200 μg dissolved in 0.9% NaCl) was injected into the lateral ventricle of the rat brain with the Hamiton syringe fixed on stereotaxic apparatus. The control rats were given with the similar volume of 0.9% NaCl. The rats were sacrified on the 1st, 3rd, 5th, 10th and 20th day after the injection of 5, 7-DHT. The results were as follows : Glial reactions induced by 5, 7-DHT were also observed in DRN. In early experimental stage, microglial reactions prevailed, whereas astroglial reactions were prevailing in later stage. In addition, microglial cells phagocytosed and removed the degenerated cells. However, astrocytes in DRN did not show phagocytotic activities such as microglial cells. Based on the results, author thought that 5, 7-DHT act as a specific neurotoxin to serotonergic neurons in DRN, and induces severe neurodegenerative changes. The glial reactions in DRN are activated during the neurodegerative changes, and show characteristic patterns of glial reactions.

6-OHDA가 흰쥐 등쪽솔기핵내 도파민성 신경세포와 별아교세포에 미치는 영향

홍은석(Eun Seog Hong),남성안(Seong Ahn Nam),조승묵(Seung Mook Jo),강태천(Tae-Cheon Kang),원무호(Moo Ho Won),최창도(Chang Do Choi) 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.4

신경독성물질인 6-hydroxydopamine (6-OHDA)이 중추신경계통 내 도파민성 신경세포에 미치는 영향과 이에 따른 별아교 세포들의 반응을 형태학적으로 규명하고자 본 연구를 시행하였다. 흰쥐의 가쪽뇌실에 6-OHDA를 투여한 후 면역조직화학 염색을 시행하여 광학현미경 하에서 등쪽솔기핵 내 도파민성 신경세포에 나타나는 퇴행성변화와 주위 별아교세포들의 반응양상 등을 경시적으로 제 3일, 제 5일, 제10일 및 제20일에서 관찰하였던 바 아래와 같은 결과를 얻었다. 6-OHDA에 의한 도파민성 신경세포의 퇴행성변화는 약물투여후 제 3일군에서 세포돌기가 팽대되고, 제 5일군과 제 10일군에서는 세포돌기의 소실과 함께 세포체가 위축되었고, 제20일군에서는 세포체가 소실되었다. 또한 6-OHDA에 의해 도파민성 신경세포들의 손상이 진행되면서 별아교세포들의 반응양상은 제 5일에서 대조군에 비해 급격하게 증가하였으며, 제20일에 이르러 최고값을 보였다. 이상의 결과로부터 6-OHDA가 중추신경계통 내 도파민성 신경세포들에 선택적이고 강한 세포독성을 유발하여 퇴행성 변화가 진행되고, 세포 손상이 시간 경과에 따라 진행되면서 주위의 별아교세포들이 지속적으로 증가된 활동을 보인다는 사실을 알게 되었다. This study was designed to clarify the cytotoxic effects of 6-hydroxydopamine (6-OHDA) on the dopaminergic neurons and astrocytes in the dorsal raphe nucleus (DRN), and to investigate neurodegenerative changes by immunohistochemistry. Adult male rats (Sprague-Dawley strain) weighing from 250 to 350 g were used as experimental animals. 6-OHDA (100 μg dissolved in 0.1% ascorbic acid) was injected into the lateral ventricle of the rat brain with the Hamilton syringe. The control rats were treated with the similar volume of 0.1 % ascorbic acid. The rats were sacrificed at the 3rd, 5th, 10th and 20th day, respectively, after the injection of 6-OHDA. The cytotoxicity of 6-OHDA resulted in severe neurodegeneration of the dopaminergic neurons in the DRN. In the 3rd day, the dopaminergic fibers were dilated. In the 5th and 10th days, the dopaminergic fibers were depleted, and dopaminergic cell bodies were shrunken. In the 20th day, the dopaminergic cell bodies were almost completely disappeared. Astroglial reactions induced by 6-OHDA were also observed in the DRN. In the 5th day, astrocytes were significantly increased as compared with that of the control value. The value were reached at its maximum by the 20th day. Based on the present results, it suggests that 6-OHDA may act as a specific neurotoxin to dopaminergic neurons in the DRN, and induce severe neurodegenerative changes. Also, it suggests that the astroglial reaction in the DRN is gradually activated during the neurodegerative changes

백서의 복강내에 투여한 Mitomycin-C-Polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

송규상,강대영,최정목,노승무,정경수,오정연,김진향,양준목,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

To evaluate the in-vivo effects of mitomycin-C-polyglycolic acid composite(MMC-PGA) the disk: shaped composite were implanted into the peritoneal cavity of the Sprague-Dawley rats. The pathologic changes were examined at various time points up to 12 weeks. Initially the soft tissue around the inserted disks showed capillary congestion at 3 days. Inflammatory infiltrates with foreign body giant cells appeared from the 2nd week and reached peak response at 6-8 weeks. These reaction diminished prominently at 12 weeks. No specific pathologic change was found in the liver, the kidneys, and the heart. The above results suggest that the MMC-PGA composite can serve as a new device for intraperitoneal chemotherapy of various types of cancers.