Research on the Characteristic of Diesel Elastic-plate Impingement Spray

( Chang Yuan Wang ),( Yong Shang ),( Xiang Rong Li ),( Fu Shui Liu ),( Cheng Hui Yu ) 한국액체미립화학회 2010 한국액체미립화학회 학술강연회 논문집 Vol.2010 No.-

A series of experimental research results on the characteristic of diesel elastic-plate impingement spray using High Speed Photography camera are presented in this paper. The experiments were carried out in a constant volume chamber specially designed, which can hold a high ambiance pressure. The special fixed device was designed so that the elastic-plate can be fixed on the spray path, meanwhile the spray incident angle and height can be changed. The free jet spray and elastic-plate impingement spray was compared under the same experimental condition including different injection pressure and different background pressure. Experimental research showed that impingement spray droplets diffuse more quickly than free jet spray.

대만의 민영화와 지방정부의 개혁 : 실무자의 평가 A Practicioner's Account.

챙, 푸-메이 Center for Local Autonomy Hanyang University 1998 민영화와 지방정부의 개혁 Vol.- No.1

Dialogue with the Giants of Microsurgery: Professor Fu-Chan Wei and Professor Joon Pio Hong

Khajuria Ankur,Chen Wei F.,Huang Jung Ju,Heredero Susana,Hong Joon-Pio,Wei Fu-Chan,Chang Tommy Nai-Jen 대한성형외과학회 2023 Archives of Plastic Surgery Vol.50 No.5

Characterization and Resistance Mechanisms of A 5-fluorouracil-resistant Hepatocellular Carcinoma Cell Line

Gu, Wei,Fang, Fan-Fu,Li, Bai,Cheng, Bin-Bin,Ling, Chang-Quan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9

Purpose: The chemoresistance of human hepatocellular carcinoma (HCC) to cytotoxic drugs, especially intrinsic or acquired multidrug resistance (MDR), still remains a major challenge in the management of HCC. In the present study, possible mechanisms involved in MDR of HCC were identified using a 5-fluorouracil (5-FU)-resistant human HCC cell line. Methods: BEL-7402/5-FU cells were established through continuous culturing parental BEL-7402 cells, imitating the pattern of chemotherapy clinically. Growth curves and chemosensitivity to cytotoxic drugs were determined by MTT assay. Doubling times, colony formation and adherence rates were calculated after cell counting. Morphological alteration, karyotype morphology, and untrastructure were assessed under optical and electron microscopes. The distribution in the cell cycle and drug efflux pump activity were measured by flow cytometry. Furthermore, expression of potential genes involved in MDR of BEL-7402/5-FU cells were detected by immunocytochemistry. Results: Compared to its parental cells, BEL-7402/5-FU cells had a prolonged doubling time, a lower mitotic index, colony efficiency and adhesive ability, and a decreased drug efflux pump activity. The resistant cells tended to grow in clusters and apparent changes of ultrastructures occurred. BEL-7402/5-FU cells presented with an increased proportion in S and G2/M phases with a concomitant decrease in G0/G1 phase. The MDR phenotype of BEL-7402/5-FU might be partly attributed to increased drug efflux pump activity via multidrug resistance protein 1 (MRP1), overexpression of thymidylate synthase (TS), resistance to apoptosis by augmentation of the Bcl-xl/Bax ratio, and intracellular adhesion medicated by E-cadherin (E-cad). P-glycoprotein (P-gp) might play a limited role in the MDR of BEL-7402/5-FU. Conclusion: Increased activity or expression of MRP1, Bcl-xl, TS, and E-cad appear to be involved in the MDR mechanism of BEL-7402/5-FU.

Establishment of the Model Widely Valid for the Melting and Vaporization Zones in Selective Laser Melting Printings Via Experimental Verifications

Chang-Shuo Chang,Kuan-Ta Wu,Chang-Fu Han,Tsung-Wen Tsai,Sung-Ho Liu,Jen-Fin Lin 한국정밀공학회 2022 International Journal of Precision Engineering and Vol.9 No.1

The thermally affected material properties operating in the three phases and porosity variations in the SS316L steel powder have been introduced to the numerical analyses for the transient volumetric heat source (Q) models developed for the solid powder, melting, and vaporization regions in the selective laser melting (SLM). The bulk Q is thus a function of these heat sources and their ratio defined for the liquid and vapor phases. The heat conduction developed for the solid powders with porosity strings the heat convection with Q as the moving heat source to solve two-dimensional temperature distributions efficiently without the confinement of operating conditions and phase presumption. The specimens with single- and multiple-track printings are prepared to investigate the effects of incident energy density (E) and power intensity (I o) on the geometries of single-track printings and the areal surface roughness (Sa) values of the multiple-track printings with 0 and 50% overlap ratios. Laser power and scanning velocity are the controlling factors for the melting pool depth D and width W . D and W become the governing factors for the keyhole with evaporations, which affects the height H of single track after solidification. The W and D results predicted by the theoretical models developed in this study have an error range, 5–20%, compared to the experimental ones, which is much lower than those reported in the literatures (Gusarov et al. in J Heat Transf 131(7):072101, 2009. https ://doi.org/10.1115/1.31092 45 ; Hussein et al. in Mater Des 52:638–647, 2013. https ://doi.org/10.1016/j.matde s.2013.05.070 ; Yin et al. Int J Adv Manuf Technol 83(9–12): 1847–1859, 2016. https ://doi.org/10.1007/s0017 0-015-7609-x ; Andreotta et al. in Finite Elem Anal Des 135: 36–43, 2017. https ://doi.org/10.1016/j.finel .2017.07.002 ). The contact angle ( ϕ * ) is defined as a function of single-track width ( W ) and solidification height ( H ). ϕ * and Sa are significantly reduced as an E is applied beyond its critical value (47.62–57.14 J/mm 3 ). Significant change in Sa is ascribed to the big difference in the morphology and its surface pattern when E or I o reaches its critical value.

Mimicking the standard model Higgs boson in UMSSM

Chang, Chun-Fu,Cheung, Kingman,Lin, Yi-Chuen,Yuan, Tzu-Chiang Springer-Verlag 2012 Journal of high energy physics Vol.2012 No.6

New Insights into the Role of Endoplasmic Reticulum Stress in Breast Cancer Metastasis

Chang-chang Han,Fu-sheng Wan 한국유방암학회 2018 Journal of breast cancer Vol.21 No.4

Cellular stress severely disrupts endoplasmic reticulum (ER) function, leading to the abnormal accumulation of unfolded or misfolded proteins in the ER and subsequent development of endoplasmic reticulum stress (ERS). To accommodate the occurrence of ERS, cells have evolved a highly conserved, selfprotecting signal transduction pathway called the unfolded protein response. Notably, ERS signaling is involved in the development of a variety of diseases and is closely related to tumor development, particularly in breast cancer. This review discusses recent research regarding associations between ERS and tumor metastasis. The information presented here will help researchers elucidate the precise mechanisms underlying ERS-mediated tumor metastasis and provide new directions for tumor therapies.

Acupotomy for scapulohumeral periarthritis

Chang-qing Guo,Shi Ning Ma,Xin Yi Fu,Quan Gui Wang,Mira Lee 대한침구의학회 2020 대한침구의학회지 Vol.37 No.4

Background: Scapulohumeral periarthritis causes pain and stiffness, and limits movement but it is a treatable condition. This was a clinical study of acupotomy treatment for scapulohumeral periarthritis. Methods: There were 80 patients randomly assigned to the traditional Chinese Medicine group (acupotomy) and the Western medicine group (naproxen), with 40 cases in each group. All patients had adjunct physiotherapy exercises for 14 days. Patients received acupotomy treatment 3 times for 14 days (Day 0, 7 and 14) or naproxen (0.22 g capsule; a non-steroidal anti-inflammatory drug) 3 times a day, for 14 days. The visual analogue scale (VAS) scores, range of motion (ROM) values, and the Melle scale, together with the therapeutic standard of diseases and syndromes in traditional Chinese Medicine were used for diagnosis and evaluation. Results: There were significant differences in the VAS scores, ROM, Melle scores, cure rate and total effective rate in the group which took naproxen and the acupotomy group, before and after treatment (p < 0.01). There were significant differences in the changes in VAS, ROM and Melle scores between the 2 groups (p < 0.01), and the acupotomy group was better than the naproxen group. Conclusion: Traditional Chinese Medicine and Western medicine can improve functional activity and reduce the level of pain experienced by patients suffering from scapulohumeral periarthritis. However, improvement of functional activity of the shoulder joint following acupotomy treatment was more obvious than the use of a non-steroidal anti-inflammatory drug, and the cure rate, and total effective rate of acupotomy was better.

Amylosporus sulcatus sp. nov. (Russulales, Basidiomycota) from Southern China

( Fu-chang Huang ),( Bin Liu ),( Hao Wu ),( Pei-sheng Qin ),( Jin-feng Li ) 한국균학회 2018 Mycobiology Vol.46 No.4

Amylosporus sulcatus sp. nov. is described from Nonggang Nature Reserve, southern China, on the basis of morphological and molecular data. The morphological description and illustrations for the new species are provided. The species is characterized by pileate and stipitate basidiocarps. The pileus surface is obviously concentrically and radiately sulcate and tomentum, and the pore surface is snow white. Phylogenetic analyses based on sequences of the internal transcribed spacer and nuclear large subunit ribosomal DNA confirmed it to be a new species.

Studies on PEGylated Gold Nanoparticles Loaded with 5-Hydroxydecanoate for Chemo-Photothermal Therapy of Human Lung Adenocarcinomas In Vitro

Fu-Yan Zhuge,CONG-WANG ZHANG,CHANG-CHUN ZENG,Han-Ping Liu 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2015 NANO Vol.10 No.7

Gold nanoparticles (Au NPs) have promising applications in the fields of drug delivery and photothermal therapy. 5-hydroxydecanoate (5-HD) is a kind of highly selective mitochondrial ATP sensitive potassium (mitoKATP) channel blocker. In this research, firstly, 5-HD was studied whether it could induce human lung adenocarcinomas (A549) cells apoptosis; secondly, PEGylated gold nanoparticles loaded with 5-HD (Au NPs-PEG-5-HD) were prepared to develop a new chemotherapy and photothermal therapy in one system under the irradiation of green light-emitting diode (LED). Subsequently, in vitro cytotoxicity test was analyzed by cell counting kit-8 (CCK-8), the change of mitochondrial membrane potential (Δψm) was determined by immunofluorescence microscopy with R-123 fluorescence, and cell apoptosis and necrosis rate were detected by flow cytometry. The results of CCK8 revealed that the inhibition rates of A549 cells were all greatly increased when cells were treated with free 5-HD, free 5-HD +LED irradiation, Au NPs-PEG-5-HD and Au NPs-PEG-5-HD+LED irradiation, and Au NPs-PEG-5-HD had enhanced cell-killing effect compared with 5-HD, furthermore, the Au NPs-PEG-5-HD and LED irradiation played a very great synergy effect. The immunofluorescence microscopy data also exhibited a reduction of Δψm correspondingly. Flow cytometric analysis showed that the apoptosis rate of cells that were incubated with free 5-HD, 5-HD+LED irradiation, Au NPsPEG-5-HD or Au NPs–PEG–5-HD+LED irradiation significantly increased to about 9.2%, 10.7%, 18.3% or 12.4% and the percentage of necrosis cells increased to around 8.8%, 9.7%, 48.0% or 69.8%, respectively. In a word, all the results indicated that the 5-HD had shown the ability to induce A549 cells apoptosis as a chemotherapy agent, and its ability would be improved when 5-HD is loaded on PEGylated Au NPs, as well as Au NPs-PEG-5-HD exhibited significantly enhanced photothermal effects for treatment of lung adenocarcinomas.