KI-180과 KI-188 칼슘조성물이 어린쥐의 성장발육에 미치는 영향

박승만(Seung-Man Park),성기승(Ki-Seung Seong),이종석(Jong-Seok Lee),이옥환(Ok-Hwan Lee),한찬규(Chan-Kyu Han) 한국식품과학회 2014 한국식품과학회지 Vol.46 No.3

본 연구에서는 KI-188 칼슘과 KI-180 조성물이 성장발육에 미치는 효과를 조사하였다. 시험기간중 KI-188 칼슘과 KI-180 식이군이 대조군 보다 성장률과 평균 식이섭취량은 증가하였다. 체장은 KI-188 칼슘과 KI-180 식이군이 대조군에 비해 평균 3.55 mm 더 길었고, 등뼈길이는 KI-180 식이군이 대조군 보다 통계적인 차이는 없었지만 0.5 mm 더 길었다. 대퇴골 무게는 KI-188 칼슘과 KI-180 식이군이 대조군에 비해 통계적으로 무거웠으며, 대퇴골길이는 대조군에 비해 각각 평균 0.89, 1.09 mm 더 길었다. 혈당, 총콜레스테롤 및 중성지방 농도는 차이가 없었고, 칼슘농도는 KI-188 칼슘과 KI-180 식이군 보다 대조군이 유의하게 높았다. 백혈구와 혈소판수는 차이가 없었고, 적혈구, 혈색소 및 헤마토크릿치는 대조군이 KI-188 칼슘과 KI-180 식이군 보다 통계적으로 높았다. ALP활성은 KI-180 식이군이 대조군 보다 통계적으로 높았고, osteocalcin농도는 KI-188 칼슘과 KI-180 식이군이 대조군에 비해 통계적으로 유의하게 높았다. 혈청 testosterone 농도는 KI-188 칼슘과 KI-180 식이군과 대조군 간에 차이가 없었다. IGF-1과 IGFBP-3 농도는 KI-180 조성물이 대조군 보다 각각 20%, 11% 정도 유의하게 더 높았다. 이상의 결과를 검토할 때 KI-188 칼슘과 KI-180 조성물은 어린쥐의 성장과 골격의 발달을 촉진시키고, 성장호르몬의 분비능을 양적으로 유도하여 전반적인 성장발육에 유효한 작용이 있는 것으로 사료된다. Herbs have active components that promote the growth rate of both animals and human. The KI-180 and KI-188 calcium food formulae contain Acanthopanacis cortex, Bombysis corpus and hoelen, seaweed calcium, chlorella extract, spirulina, colostrum powder, and other natural and functional components. We evaluated the growth-promoting effects of these formulae by analyzing the weight, femur and backbone, alkaline phosphatase, osteocalcin, testosterone, insulin-like growth factor (IGF)-1, and IGF binding protein-3 (IGFBP-3) of growing rats. Growing rats administered with KI-180 and KI-188 calcium showed the increase of body weight, body length, and femur weight and length of growing rats. In addition, administration of KI-180 and KI-188 calcium increased the alkaline phosphatase activity, the levels of osteocalcin and the growth hormones IGF-1 and IGFBP-3 of growing rats. The impact of KI-180 and KI-188 calcium on the physical development of growing rats suggests that the incorporation of these food formulae in the diets of growing children may promote the physical development.

인체 대장암 및 자궁경부암에서 PCR-SSCP법을 이용한 Ki-ras 암유전자의 점돌연변이에 관한 연구

박영홍,백낙환,김현찬,김상효,홍관희,김기태,이기영 인제대학교 1994 仁濟醫學 Vol.15 No.2

인체 대장암 20례와 자궁경부암 10례의 암조직을 대상으로 c-Ki-ras 유전자 codon 12와 13에서의 점돌연변이를 알아보고자 polymerase chain reaction(PCR)과 single-strand conformation polymorphism(SSCP) 검사법을 시행하였다. 대장암의 경우 20례중 9례(45%)에서, 자궁경부암의 경우 10례중 1례(10%)에서 양성으로 나왔다. 대장암에서는 codon 12에서 GGT가 TGT로의 치환이 4례로서 가장 많았으며, AGT, CGT로의 치환이 각 1례였고 codon 13에서는 GGC가 GAC로의 치환이 2례, TGC로의 치환이 1례인 것으로 나타났다. 자궁경부암의 경우 codon 12에서 GGT가 AGT로의 치환이 1례인 것으로 나타났다. In an attempt to clarify the role of genetic alteration in the genesis of human colorectal and cervical cancers, tissue specimens from 20 patients with colorectal cancer and 10 patients with cervical cancer were examined for the presence of point mutation in K-ras2 exon 1 by single strand conformation polymorphism analysis of PCR product. Exon I of c-Ki-ras2 was amplified by polymerase chain reaction(PCR) and comparison was made between the normal and mutated genes by nondenaturing polyacrylamide gel electrophoresis(PAGE) of PCR product and nucleotide sequence analysis using asymmetric PCR with direct sequencing. Genomic DNA from white blood cells were used as normal control and those from A427 cell line were used as mutated control. 1.PCR product from A427 cell line showed a distinct migration shift pattern compared to the normal control in PAGE and the direct sequencing indicated that nucleotide sequence of codon 12 was mutated from GGT to GAT. In addition, there was a loss of normal allele in A427 cell line. 2.PCR product from 9 cases(45%) out of a 20 colon cancer patients showed migration shifts in PAGE, and all of these 9 patients invariably demonstrated mutation of ras gene, either in codon 12(6 cases:30%) or 13(3 cases:15%). The base substitutions in codon 12 were: from GGT to AGT(1), CGT(1), or TGT(4). Base changes in codon 13 were from GGC to TGC(1) or GAC(2). 3.PCR product from 1 cases(10%) out of a 10 cervical cancer patients showed migration shrifts in PAGE, Invariably demonstrated mutation of ras gene in codon 12. The base substitutions was from GGT to AGT(1) TQE incidence of point mutation of c-Ki-ras in colon cancer was high, however, it was low in cervical cancer, which showed the relation of ruts gene mutation with colon cancer. PCR -SSCP analysis is a simple, rapid and efficient method of detection of point mutation, especially when dealing with multiple samples.

단재 문학의 국수론(國粹論)

김찬기(Kim, Chan?Ki) 고려대학교 한국학연구소 2016 한국학연구 Vol.57 No.-

이 연구에서는 단재(1880~1936) 문학에 나타난 국수론(國粹論)의 함의를 탐색하고, 이를 토대로 하여 우리 근대 민족문학사의 한 계보를 형성하고 있는 국수(國粹) 서사에 대한 종합적 이해의 시각을 마련하고자 한다. 또한, 이 연구를 통해 단재 이후의 민족문학의 성격을 다양한 부면에서 이해할 수 있는 토대를 마련할 수 있게 될 것이다. 근대 초기 단재는 ‘Nationalism’의 번역어로 ‘국수론’을 제출하였고, 단재에게 그것은 어떤 식으로든 서구 근대성과 대타적 관계 설정이 가능한 이데올로기였다. 이 연구에서는 서구 근대성 비판의 맥락, 그리고 이것과 서로소 관계에 있었던 근대 초기의 (고)조선주의 이데올로기를 국수론(國粹論)과 관련하여 탐색함으로써 우리 근대문학사의 근대성 비판의 계보학 연구에 관한 새로운 이해의 시각을 제공할 것이다. This paper have studied the “Nationalism(國粹論)” of Dan-Jae’s(1880~1936) literature. In addition, this paper deals with the various features of the early modern “Nationalism(國粹論)”particularly examining how Dan-Jae’s nationalism interacted with the Western modernity and Confucianism. And through this study, we are able to understand a characteristic of Dan-Jae’s nationalism. Dan-Jae understood “Nationalism” as a counter discourse against Western modernity and Confucianism. It was a concept that was a confrontational relationship with Western modernity and Confucianism. In addition, this essay investigated the context of Western modernity criticism and Confucianism criticism. And this paper will provide us with the vision of a new understanding on the genealogy of Western modernity criticism in korean modern literature.

개에서 발생한 수컷 가성 반음양 1례

이기찬,손정민,윤기영,최호정,박성준,이영원,정성목,신상태,조종기 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2006 動物醫科學硏究誌 Vol.14 No.-

In this report, we present a case of the male pseudohermaphrodite in a beagle dog. The animal was subjected for examination due to enlargement of clitoris that was shown as reddish finger-like structure protruding from the vulva. Visual examination of the internal genitalia revealed that the animal possessed both testes and uterus. Based on the findings of physical examination and exploratory laparotomy, it was tentatively diagnosed as male pseudohermaphrodite. Internal genitalia and enlarged clitoris were removed for the prevention of pyometra and Sertoli cell tumor.

원발성 피부 CD30 양성/ALK 음성 퇴형성 대세포 림프종 1예

이은주,김협,서영준,서기범,이증훈,박장규,김유찬,김춘옥 대한피부과학회 2003 大韓皮膚科學會誌 Vol.41 No.5

Primary cutaneous CD3O(Ki-1) Positive anaplastic large cell Iymphoma(ALCL) is a rare subset of cutaneous Iymphoma. with a much better prognosis. ALCL is a heterogeneous process that may have a T-cell, B-cell, or indeterminant(null) phenotype and which may or may not express the anaplastic Iymphoma kinase(ALK) oncoprotein. We report a case of ALCL in a 72 year old man. About 4 months ago, multiple erythematous firm ulcerative mass and satellite nodules developed on the right lower leg. The skin lesions rapidly increased in number and size. Some lesions became painful and centrally ulcered. The histologic findings showed a diffuse infiltrate of large Iymphocytes with large nuclei, prominent and multiple nucleoli, and ample cytoplasm. Immunohistochemical stainings for CD3O, CD5 were positive but stainings for LCA, CD3, CD45RO, CD2O, cytokeratin, EMA, and ALK were negative. Therefore, we diagnosed our case as CD+/30ALK- ALCL (Korean J Dermatol 2003;41(5) : 666~669)

인삼양위탕(人蔘養胃湯)의 임상활용(臨床活用)에 대(對)한 형상의학적(形象醫學的) 고찰(考察)

박찬기,Park Chan-Ki 대한한의학방제학회 2003 大韓韓醫學方劑學會誌 Vol.11 No.1

A general review is made on Insamyangwitang(人蔘養胃湯). Following conclusions are drawn from the clinical cases of Insamyangwitang in Hyungsang medicine. 1. Insamyangwitang is composed of four different prescriptions of Huisaentang, Sakoonjatang, Eajintang and Pyungwisan. Huisaentang is usually prescribed for the intestinal convulsion. Sakoonjatang for the deficiency of Ki. Eajintang for retention of phlegm. 2. Insamyangwitang is effective in strengthening the spleen, drying the dampness, warning the middle-warmer to stop vomiting, regulating the flow of Ki, and eliminating phlegm. 3. Insamyangwitang is applicable to malaria caused by cold, intestinal convulsion, abdominal mass, edema, tympanites, Yin syndrome of exogenous febrile disease, distension, lack of appetite, stomachache, and diarrhea. 4. Persons with the following characteristic in Hyungsang are more susceptible to Insamyangwitang : Jung type, Hyul type, fish type, Taium meridian type, white fat damp constitution, person with big mouth, and woman rather than man.

항암화학요법 중 호중구감소증이 발생한 저위험군 발열 환자들을 대상으로 한 경구 항균제 요법의 임상적 유용성 및 안정성에 대한 연구

김연숙,이혁,기현균,김춘관,김신우,김성민,백경란,김원석,윤성수,이홍기,강원기,박찬형,박근칠,송재훈 대한화학요법학회 2000 대한화학요법학회지 Vol.18 No.1

목적 : 항암화학요법 중 호중구감소증을 동반한 발열이 발생하는 암환자들을 치료하기 위한 다양한 항균제와 여러 가지 방법들이 시도되고 있는 가운데, 합병증과 사망률의 발생가능성이 적은 저위험군 환자들을 대상으로 초기 72시간동안 정주 항균제를 투여한 이후 경구 항균제로 전환하는 요법의 유용성과 안정성을 평가해보고자 본 연구를 시행하였다. 방법 : 1998년 2월부터 1999년 9월까지 본원에서 항암화학요법 중 호중구감소증과 발열이 발생한 환자들 가운데 기저 암질환이 고형암이거나 림프종이고, 입원당시 패혈증의 증후가 없으며 입원 72시간이내에 해열되고 백혈구수치가 증가 추세인 환자들을 대상으로 하여 72시간 동안 정주 항균제를 투여한 이후 경우 ciprofloxacin 750㎎을 하루 2회씩 투여하여 총 4일간 투여하였다. 모든 환자들은 열이 떨어지고 호중구감소증이 회복될 때까지 입원하도록 하였다. 결과 : 총 38명 환자의 40예가 등록이 되었고, 환자들의 기저암 질환은 고형함이 72.5%, 림프종이 27.5%였다. 입원당시 평균 호중구치수는 156/㎕였고, 호중구수치가 100/㎕미만인 경우는 65%였으며, 호중구감소증이 지속된 기간의 평균은 2.4일이었다. 40예 중 39예가 항균제의 변형이나 추가 없이 호중구감소증과 발열로부터 회복이 되어 97.5%의 성공율(95% 신뢰구간: 86.8-99.9%)을 보였다. 부작용으로 피부발진이 있었던 경우가 한 예 있었는데, 증상이 경하여 경구 항균제를 지속할 수 있었다. 심와부의 동통으로 복용을 지속할 수 없어서 대상에서 제외된 예가 또 한 예 있었다. 결론 : 항암요법 중 호중구감소증과 발열을 동반한 환자들 가운데 저위험군 환자들에서 항균제 72시간정주 이후 경구 항균제로의 전환요법은 효과적이고도 안전한 치료방법이라고 할 수 있다. Background : Oral antibiotic therapy following empirical intravenous antibiotics may be effective and safe for febrile neutropenic patients with lowrisk for complications. Methods : We conducted a prospective clinical trial of oral antibiotic therapy in the patients with neutropenia and fever during chemotherapy for cancer. Underlying malignancies were solid tumor or lymphoma with short duration of neurtropenia and the patients had no evidence of clinically or microbiologically documented infections. Oral ciprofloxacin was given to the patients who lacked signs of sepsis on admission, had a rising tendency of neutrophil count (ANC >100 /㎕ ) at 72 hours, and were afebrile at 72 hours. All patients were hospitalized until neutropenia and fever resolved. Results : A total of 40 episodes of 38 patients were enrolled from February 1998 to September 1999. The mean neutrophil counts on admission were 156/㎕ and the mean duration of neutropenia was 2.4 days. The episodes which had neutrophil count below 100 /㎕ were 26 (65%). Treatment was successful in 39 of 40 episodes (97.5% : 95 % confidence interval, 86.8% to 99.9%). Adverse reactions of oral ciprofloxacin were skin rash and epigastric soreness in two cases, respectively. There were no deaths during the study. Conclusions : For low-risk febrile patients with neutropenia during cancer chemotherapy, switch therapy to oral ciprofloxacin at 72 hours following intravenous broad-spectrum antibiotics is effective and safe,

農村地域主婦의 諸特性이 保健醫療에 미치는 影響

寄老錫,朴贊云 全北大學校 醫科大學附設 看護專門大學 1980 논문집 Vol.6 No.-

A multiple regression analysis was conducted in order to determine the effects of characteristics of rural housewives (age, education, occupation, and number of children), and characteristics of household (economic status, cultural level, and contact of health worker) on health status of family member and utilization medical cares. The data were collected from 533 rural housewives in Jeon-bug area for this analysis. The results summarized are follows : 1. The housewives' age, number of children, and occupation had positive correlation to prevalence of disease of family members, but the educational level had significant negative correlation. 2. Housewives' age, education, number of children and occupation have 16.8 per cent variable explained on health status. Multiple regression coefficients by age, education, number of children and occupation was 0.296, -0.207, 0.096 and 0.319 respectively. 3. Economic status, the frequency of health worker contact and health status have reciprocal positive coorelation. 4. Economic status, frequency of worker contact and cultural level have 19.8 per cent variable explained on health level of family members, Multiple regression coefficient of economic status, cultural status and contact with worker was -0.410, 0.171, and -0.273 respectively. 5. The selected variables on health status by stepwise regression analysis was 5.95 per cent variable explained in contact wit health worker, 10 per cent in economic status, and the contribution proportion of economic status was 4.3 per cent. 6. Health status(Prevalence of disease) and utilization of medical care had significant positive correlation(r=+0.42).

조혈모세포이식환자에서 조혈기능의 회복과 관련된 인자들에 관한 연구

정기주,강명수,권기두,김경하,이상철,김현정,배상병,김찬규,이남수,이규택,원종호,홍대식,박희숙 순천향대학교 의학연구소 2007 Journal of Soonchunhyang Medical Science Vol.13 No.1

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오존노출시간 및 희생시기에 따른 백서 비점막의 형태학적 변화

나기상,김병국,김용민,박찬일 충남대학교 의과대학 지역사회의학연구소 2000 충남의대잡지 Vol.27 No.1

Ozone causes epithelial injury in the upper and lower respiratory tract of laboratory animals. Although there has been good characterization of the ozone induced lesions in the nasal mucosa, it's not clear that morphologic changes depend on the concentration of ozone and time of exposure. And also it's not clear that there is a change between time of sacrifice after last exposure, too. This study was designed to compare the morphological changes in the nasal mucosa in rats exposed to 0.6 and 1.2ppm with different time of exposure and sacrifice after last exposure. We observed infiltration of neutrophils, increased goblet cells, epithelial hyperplasia and ciliary loss in the groups that exposed for 3 and 7days. The degree of changes increased in the groups exposed for 7days than 3days. But there was no difference between the groups sacrificed in immediately and 18hours after last exposure except infiltration of neutrophils. These findings were similar to the groups exposed to 0.6 and 1.2ppm.