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Jiří Killer,Chahrazed Mekadim,Radko Pechar,Věra Bunešová,Eva Vlková 한국미생물학회 2018 The journal of microbiology Vol.56 No.10
In the modern era, molecular genetic techniques are crucial in ecological studies, as well as in the classification, typing, and phylogenetic analysis of prokaryotes. These techniques are mainly aimed at whole genome comparisons and PCRderived experiments, including amplifying the 16S rRNA and other various housekeeping genes used in taxonomy, as well as MLST (multilocus sequence typing) and MLSA (multilocus sequence analysis) of different taxonomic bacterial groups. The gene encoding threonine-tRNA ligase (thrS) is a gene potentially applicable as an identification and phylogenetic marker in bacteria. It is widely distributed in bacterial genomes and is subject to evolutionary selection pressure due to its important function in protein synthesis. In this study, specific primers were used to amplify a thrS gene fragment (~740 bp) in 36 type and 30 wild strains classified under family Bifidobacteriaceae. The full-length gene has not yet been considered as a possible identification, classification, and phylogenetic marker in bifidobacteria. The thrS sequences revealed higher sequence variability (82.7% of pairwise identities) among members of the family than that shown by 16S rRNA gene sequences (96.0%). Although discrepancies were found between the thrS-derived and previously reported whole genome phylogenetic analyses, the main phylogenetic groups of bifidobacteria were properly assigned. Most wild strains of bifidobacteria were better differentiated based on their thrS sequences than on their 16S rRNA gene identities. Phylogenetic confidence of the evaluated gene with respect to other alternative genetic markers widely used in taxonomy of bifidobacteria (fusA, GroELhsp60, pyrG, and rplB genes) was confirmed using the localized incongruence difference - Templeton analysis.
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Karima Belarbi,Fawzia Atik-Bekkara,Imad Abdelhamid El Haci,Ilhem Bensaid,Fawzia Beddou,Chahrazed Bekhechi 경희대학교 융합한의과학연구소 2017 Oriental Pharmacy and Experimental Medicine Vol.17 No.2
Teucrium pseudo-Scorodonia Desf., is one of many Mediterranean plants widely used in Algerian folk medicine for their medicinal properties. This study represents the first antioxidant investigation of Teucrium pseudo-Scorodonia with different analytical methods. Total phenolic, flavonoid, and condensed tannin contents were determined using spectrophotometric techniques. In vitro antioxidant and radical scavenging profiling was determined through total antioxidant capacity (TAC), DPPH radical-scavenging, ferric reducing antioxidant power (FRAP), and β-carotene bleaching assays. Leaf extracts exhibited high total phenolics (266.36 ± 0.14 mg GAE/g DW), flavonoids (161.23 ± 0.18 mg CE/g DW) and condensed tannins (5.00 ± 0.08 mg CE/g DW) contents. The greatest antioxidant activity was founding in the butanolic fraction of leaves (IC50.DPPH = 0.05 ± 0.00 mg/ml) followed by crude methanolic extract and ethyl acetate fraction. These values were to some extent high in comparison to the positive control (0.07 ± 0.01 mg/ml). The same tendency was observed with ferric reducing power. A strong correlation of IC50 values of antioxidant assays with total phenolics and total flavonoids content of T. pseudo-Scorodonia was exhibited in this study. Thin layer chromatographic (TLC) screening using DPPH radical, AlCl3 and FeCl3 as detection reagents led to the identification of potent antioxidant compounds: methylated flavonoids or hydroxyflavonols. These results indicate that flavonoids and phenolics can be the major contributors to the antioxidant activity observed for the T. pseudo-Scorodonia leaf extracts. Further study is necessary for isolation and characterization of the active antioxidants, which may serve as a potential source of natural antioxidants.
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Ilhem Bensaid,Fawzia Atik Bekkara,Imad Abdelhamid El Haci,Karima Belarbi,Fawzia Beddou,Chahrazed Bekhechi 경희대학교 융합한의과학연구소 2017 Oriental Pharmacy and Experimental Medicine Vol.17 No.4
The valorization of the local flora is the goal of the laboratory of Natural Products from the University of Tlemcen (Algeria). In this work, we report the scientific study of a medicinal plant Cynoglossum cheirifolium L. The crude methanol and tannin extracts of C. cheirifolium were preliminary analyzed for their phenolic contents (total phenolics, total flavonoids and total tannins). These extracts were evaluated for their antioxidant properties using different methods. The results showed that crude methanolic extract of the leaves part revealed high phenolic (134.93 ± 9.76 mg Gallic Acid Equivalent/g Dry Weigth), flavonoid (99.99 ± 0.72 mg Catechin Equivalent/g Dry Weigth) and condensed tannin contents (1.65 ± 0.05 mg Catechin Equivalent/g Dry Weigth). Furthermore, the results obtained from the study of the antioxidant activity showed that tannin extract of stems displayed the highest DPPH• scavenging ability with the inhibition concentration of 50% (IC50) value of 70 ± 1 μg/mL, it was comparable to the Vitamin E with an IC50 value of 60 ± 1 μg/mL. The vitamin E concentration required to reduce the ferric iron was lower (242 ± 6 μg/mL) than tannins extracted from leaves (214 ± 4 μg/mL). Hence, using an RP-HPLC-PDA (Reverse Phase-High Pressure Liquid Chromatography-PhotoDiode Array Detector) analysis, it was possible to identify phenolic acids and flavonoid like rutin; naringenin; rosmarinic acid; sinapic acid; p-coumaric acid; ferulic acid; caffeic acid and syringic acid.
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SURFACES OF REVOLUTION SATISFYING △IIG = f(G + C)
Chahrazede Baba-Hamed,Mohammed Bekkar 대한수학회 2013 대한수학회보 Vol.50 No.4
In this paper, we study surfaces of revolution without par- abolic points in 3-Euclidean space R3, satisfying the condition △IIG = f(G+ C), where △II is the Laplace operator with respect to the second fundamental form, f is a smooth function on the surface and C is a con- stant vector. Our main results state that surfaces of revolution without parabolic points in R3 which satisfy the condition △IIG = fG, coincide with surfaces of revolution with non-zero constant Gaussian curvature.
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SURFACES OF REVOLUTION SATISFYING Δ<sup>II</sup>G = f(G + C)
Baba-Hamed, Chahrazede,Bekkar, Mohammed Korean Mathematical Society 2013 대한수학회보 Vol.50 No.4
In this paper, we study surfaces of revolution without parabolic points in 3-Euclidean space $\mathbb{R}^3$, satisfying the condition ${\Delta}^{II}G=f(G+C)$, where ${\Delta}^{II}$ is the Laplace operator with respect to the second fundamental form, $f$ is a smooth function on the surface and C is a constant vector. Our main results state that surfaces of revolution without parabolic points in $\mathbb{R}^3$ which satisfy the condition ${\Delta}^{II}G=fG$, coincide with surfaces of revolution with non-zero constant Gaussian curvature.
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