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Prasad Andhare,Dweipayan Goswami,Ce´dric Delattre,Guillaume Pierre,Philippe Michaud,Hilor Pathak 한국응용생명화학회 2017 Applied Biological Chemistry (Appl Biol Chem) Vol.60 No.3
Succinoglycan is an industrially important exopolysaccharide (EPS) that is produced by certain bacteria. There are several procedures to extract this EPS, though the efficiency of all the available procedures is questionable and any improvement in the extraction efficient can greatly benefit the industry. Here we emphasize on optimization and development of new modus operandi to efficiently extract succinoglycan from liquid bacterial culture. Also, we studied the effect of different extraction methods on production, rheological and structural properties of succinoglycan. Eighteen different chemical and physical methods were tested for succinoglycan extraction from Rhizobium radiobacter CAS isolates with the principle of extracting EPS by precipitating it, where only eleven methods could precipitate the succinoglycan. Comparing the extraction yield of all methods, biopolymer extracted by acetone (3014 mg/L) was maximum followed by cetyl-trimethyl-ammonium-bromide (CTAB 2939 mg/ L) and vacuum evaporation (2804 mg/L) methods. Upon comparison of rheological property of recovered succinoglycan, it was found that at shear rate 50 s-1 EPS recovered using acetone and CTAB methods tends to make the solution highly viscous with a viscosity of 150 and 146 mPa s, respectively. In agreement with these results, power law equation showed that EPS extracted by acetone and CTAB had high consistency index (k) and low flow behavior index (g). The current results showed that the physicochemical methods for EPS extraction significantly affect the structural composition of, though succinoglycan extracted using acetone and CTAB showed minimum structural abrasion.
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Velamo do Campo: Its Volatile Constituents, Secretory Elements, and Biological Activity
Fatiha El Babili,Christine Roques,Laila Haddioui,Floriant Bellvert,Ce´dric Bertrand,Christian Chatelain 한국식품영양과학회 2012 Journal of medicinal food Vol.15 No.7
The volatile components from Croton campestris root bark were localized by an anatomical study and analyzed by gas chromatography–mass spectrometry for the first time. The roots of this plant showed secretory cells. These volatile constituents, isolated from the dichloromethane extract by hydrodistillation, were analyzed by gas chromatography–mass spectrometry. We found 69 components. They were characterized, and the major constituents of crude oil root barks were spathulenol (23.3%) and borneol (18.7%). Growth inhibitory activity of the active compounds in solution was evaluated by measuring minimal inhibitory concentrations using a broth micromethod. The minimal inhibitory concentration of root bark volatile constituents was 1.56 lg/mL for Staphylococcus aureus, 3.125 lg/mL for Candida albicans, and 6.25 lg/mL for Aspergillusniger.
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