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Nanda Chakraborty,심성철,Michael Casler,정근화 한국원예학회 2014 Horticulture, Environment, and Biotechnology Vol.55 No.4
Dollar spot (caused by Sclerotinia homoeocarpa F. T. Bennett) is the most economically important fungalturf disease on golf courses in North America. This disease is mainly controlled by using multiple fungicides. However,the causal fungus has developed resistance to seveal classes of fungicides and host resistance is an alternative controlstrategy. A creeping bentgrass (Agrostis stolonifera L.) cultivar improved with dollar spot resistance would greatly reducethe costs and environmental impacts of fungicide applications. Our previous QTL analysis in the creeping bentgrass‘549 × 372’ population detected one QTL with a large effect for dollar spot resistance and several QTLs with smallereffects. One RAPD primer, AW10, amplified an 650-bp band in the parent ‘372’, was named 3.AW10.650. This RAPDmarker was located on linkage group 7.1 and was significantly (p < 0.0001) associated with the QTL for dollar spotresistance based on 697 progeny of the ‘549 × 372’ population. In this study, we converted the RAPD marker 3.AW10.650into a sequence characterized amplified region (SCAR) marker, named CreepDSp.650. The SCAR marker showed asignificant association (p = 0.006) with dollar spot resistance in an additional segregating population developed at theUniversity of Illinois. We report here the first PCR-based SCAR marker associated with a major-effect QTL for dollarspot resistance in creeping bentgrass. This SCAR marker will facilitate evaluating relatively large numbers of breedingprogeny for marker-assisted selection (MAS) in bentgrasses.