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Direct Adventitious Shoot Formation on Seedling Radicles in Seed Cultures of Strawberry
Kirschbaum, Daniel S.,Cantliffe, Daniel J.,Shaw, Nicole L.,Liu, Jang R. 한국식물학회 2004 Journal of Plant Biology Vol.47 No.2
Mature seeds of strawberry (Fragaria x ananassa) were placed on Murashige and Skoog medium supplemented with 2.22 μM 6-benzyladenine. After four weeks of culture, and without an intervening callus phase, approximately 36% of the resulting seedling radicles had formed numerous adventitious buds near their tips. A few buds on each radicle developed into shoots, while others formed disorganized calli. Consequently, the seedlings exhibited shoot apices at both ends of the axis of polarity. Our overall results suggest that a considerable level of plasticity in organ determination occurs even in higher plants, and that exogenous growth regulators can cause a root primordium in the radicle to be converted to a shoot primordium.
고구마 ( Ipomoea batatas ) 의 현탁배양 (縣濁培養) 세포의 원형질체 배양에 의한 캘러스 형성
유장열,Daniel J . Cantliffe (Jang R . Liu) 한국식물학회 1989 Journal of Plant Biology Vol.32 No.4
Protoplasts were enzymatically isolated from suspension cltures of sweet potato. High yields of single protoplasts were produced from nonembryogenic cell aggregates. However, most protoplasts obtained from embryogenic cell clumps were spontaneously fused during enzyme treatment; a small portion of them remained single. Upon transfer to Murashige and Skoog`s(MS) liquid medium supplemented with 0.1 ㎎/l 6-benzyladenine(BA) and 1 ㎎/l 2,4-dichlorophenoxyacetic acid(2,4-D), protoplasts from nonembryogenic cell aggregates sustained cell divisions to form callus. Upon subculture onto MS media with 0.2 ㎎/l 2,4-D or without growth regulators, the callus did not give rise to any organs. On the other hand, first cell division of single protoplasts from embryogenic cell clumps was sporadically observed.