Converting Panax ginseng DNA and chemical fingerprints into two-dimensional barcode

Cai, Yong,Li, Peng,Li, Xi-Wen,Zhao, Jing,Chen, Hai,Yang, Qing,Hu, Hao The Korean Society of Ginseng 2017 Journal of Ginseng Research Vol.41 No.3

Background: In this study, we investigated how to convert the Panax ginseng DNA sequence code and chemical fingerprints into a two-dimensional code. In order to improve the compression efficiency, GATC2Bytes and digital merger compression algorithms are proposed. Methods: HPLC chemical fingerprint data of 10 groups of P. ginseng from Northeast China and the internal transcribed spacer 2 (ITS2) sequence code as the DNA sequence code were ready for conversion. In order to convert such data into a two-dimensional code, the following six steps were performed: First, the chemical fingerprint characteristic data sets were obtained through the inflection filtering algorithm. Second, precompression processing of such data sets is undertaken. Third, precompression processing was undertaken with the P. ginseng DNA (ITS2) sequence codes. Fourth, the precompressed chemical fingerprint data and the DNA (ITS2) sequence code were combined in accordance with the set data format. Such combined data can be compressed by Zlib, an open source data compression algorithm. Finally, the compressed data generated a two-dimensional code called a quick response code (QR code). Results: Through the abovementioned converting process, it can be found that the number of bytes needed for storing P. ginseng chemical fingerprints and its DNA (ITS2) sequence code can be greatly reduced. After GTCA2Bytes algorithm processing, the ITS2 compression rate reaches 75% and the chemical fingerprint compression rate exceeds 99.65% via filtration and digital merger compression algorithm processing. Therefore, the overall compression ratio even exceeds 99.36%. The capacity of the formed QR code is around 0.5k, which can easily and successfully be read and identified by any smartphone. Conclusion: P. ginseng chemical fingerprints and its DNA (ITS2) sequence code can form a QR code after data processing, and therefore the QR code can be a perfect carrier of the authenticity and quality of P. ginseng information. This study provides a theoretical basis for the development of a quality traceability system of traditional Chinese medicine based on a two-dimensional code.

Effect of Withaferin A on A549 Cellular Proliferation and Apoptosis in Non-small Cell Lung Cancer

Cai, Yong,Sheng, Zhao-Ying,Chen, Yun,Bai, Chong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.4

Objective: To explore the effect of Withaferin A on A549 cellular proliferation and apoptosis in non-small cell lung cancer (NSCLC). Materials and Methods: NSCLC cell line A549 was selected to explore the effect of Withaferin A on A549 cellular proliferation, apoptosis and the PI3K/Akt signal pathway capable of regulating tumor biological behavior by assessment of cellular proliferation, cellular apoptotic rates and cellular cycling as well as by immuno-blotting. Results: Withaferin A could inhibit A549 cellular proliferation and the control rate was dosage-dependent (P<0.05), which also increased time-dependently with the same dosage of Withaferin A (P<0.05). The apoptotic indexes in A549 cells treated with 0, 2.5, 5.0, 10.0 and 20.0 ${\mu}mol{\cdot}L^{-1}$ Withaferin A for 48 h were significantly different (P<0.05). In addition, the apoptotic rates of each group in both early and advanced stages were higher than those in 0 ${\mu}mol{\cdot}L^{-1}$ (P<0.05), which were evidently higher after 48 h than those after 24 h (P<0.05). A549 cells treated by Withaferin A for 48 h were markedly lower in Bcl-2 level and obviously higher in Bax and cleaved caspase-3 levels than those treated by 0 ${\mu}mol{\cdot}L^{-1}$ Withaferin A (P<0.05), and there were significant differences among 5, 10 and 20 ${\mu}mol{\cdot}L^{-1}$ Withaferin A (P<0.05). The ratios of A549 cells treated by Withaferin A for 48 h in G0/G1 stage were higher than those in 0 ${\mu}mol{\cdot}L^{-1}$, while those in S and G2/M stages were obviously lower than those in G2/M stage, and there were significant differences in 5.0, 10.0 and 20.0 ${\mu}mol{\cdot}L^{-1}$ Withaferin A (P<0.05). Additionally, p-Akt/Akt values were in reverse association with dosage, and the differences were significant (P<0.05). Conclusion: Withaferin A can inhibit the proliferation and apoptosis of A549 cells by suppressing activation of the PI3K/Akt pathways.

Clinical Observation of Whole Brain Radiotherapy Concomitant with Targeted Therapy for Brain Metastasis in Non-small Cell Lung Cancer Patients with Chemotherapy Failure

Cai, Yong,Wang, Ji-Ying,Liu, Hui Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

Objective: To investigate the clinical effects of whole brain radiotherapy concomitant with targeted therapy for brain metastasis in non-small cell lung cancer (NSCLC) patients with chemotherapy failure. Materials and Methods: Of the 157 NSCLC patients with chemotherapy failure followed by brain metastasis admitted in our hospital from January 2009 to August 2012, the combination group (65 cases) were treated with EGFR-TKI combined with whole brain radiotherapy while the radiotherapy group (92 cases) were given whole brain radiotherapy only. Short-term effects were evaluated based on the increased MRI in brain 1 month after whole brain radiotherapy. Intracranial hypertension responses, hematological toxicity reactions and clinical effects of both groups were observed. Results: There were more adverse reactions in the combination group than in radiotherapy group, but no significant differences were observed between the two groups in response rate (RR) and disease control rate (DCR) (P>0.05). Medium progression free survival (PFS), medium overall survival (OS) and 1-year survival rate in combination group were 6.0 months, 10.6 months and 42.3%, while in the radiotherapy group they were 3.4 months, 7.7 months and 28.0%, respectively, which indicated that there were significant differences in PFS and OS between the two groups (P<0.05). Additionally, RPA grading of each factor in the combination group was a risk factor closely related with survival, with medium PFS in EGFR and KRAS mutation patients being 8.2 months and 11.2 months, and OS being 3.6 months and 6.3 months, respectively. Conclusions: Whole brain radiotherapy concomitant with target therapy is favorable for adverse reaction tolerance and clinical effects, being superior in treating brain metastasis in NSCLC patients with chemotherapy failure and thus deserves to be widely applied in the clinic.

Radiosensitization Effect of Overexpression of Adenovirus-mediated SIRT6 on A549 Non-small Cell Lung Cancer Cells

Cai, Yong,Sheng, Zhao-Ying,Liang, Shi-Xiong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.17

Objective: To explore the radiosensitization effect of overexpression of silent information regulator 6 (SIRT6) on A549 non-small cell lung cancer (NSCLC) cells. Methods: Adenovirus vector Ad-SIRT6 causing overexpression of SIRT6 was established. Western blotting and MTT assay were adopted to detect the level of SIRT6 protein and the inhibitory rate of A549 cell proliferation after different concentrations of adenovirus transduction (0, 25, 100, 200, and 400 pfu/cell) for 24 h. Control group, Ad-null group and Ad-SIRT6 group were designed in this experiment and virus concentration of the latter two groups was 200 pfu/cell. Colony formation assays were employed to test survival fraction (SF) of the 3 groups after 0, 2, 4, 6, 8, 10 X-ray irradiation. Flow cytometry was used to detect the status of cell cycle of 3 groups after 48 h of 4Gy X-ray irradiation and Western blotting was used to determine the expression of apoptosis-related genes of 3 groups after 48 h of 4GyX-ray irradiation. Results: In the range of 25~400 pfu/cell, the inhibitory rate of A549 cell proliferation increased as adenovirus concentration raised. The inhibitory rates under the concentrations of 0, 25, 100, 200, and 400 pfu/cell were 0%, $4.23{\pm}0.34%$, $12.7{\pm}2.57%$, $22.6{\pm}3.38%$, $32.2{\pm}3.22%$, $38.7{\pm}4.09%$ and $47.8{\pm}5.58%$ and there were significantly differences among groups (P<0.05). SF in Ad-SIRT6 group was lower than Ad-null and control groups after 4~10Gy X-ray irradiation (P<0.05) and the sensitization enhancement ratio (SER) was 1.35 when compared with control group. Moreover, after 48 h of 4Gy X-ray irradiation, there appeared a significant increase in G1-phase cell proportion, upregulated expression of the level of apoptosis-promoting genes (Bax and Cleaved caspase-3), but a obvious decline in S-phase and G2-phase cell proportion and a significant decrease of the level of apoptosis-inhibiting gene (Bal-2) in the Ad-SIRT6 group (P<0.05). Conclusion: The over-expression of adenovirus-mediated SIRT6, which has radiosensitization effect on A549 cells of NSCLC, can inhibit the proliferation of A549 cells and cause G0/G1 phase retardation as well as induce apoptosis of cells.

Vibration suppression control for AMB system based on static learning process and feedforward current compensation

Caiyong Ye,Jintao lu,Shanming Wan,Xiaodong Qi 전력전자학회 2024 JOURNAL OF POWER ELECTRONICS Vol.24 No.4

Synchronous vibration caused by rotor eccentricity is an important obstacle to the development of an active magnetic bearing (AMB) system. To solve this problem, a novel autobalancing control strategy has been proposed, which includes a static learning process and dynamic feedforward current injection process. In this algorithm, a dynamic response process under unbalanced force is simulated by a static learning process. Then, a transfer function containing amplitude and phase information is obtained. According to the obtained transfer function information, a specific feedforward current is constructed to eliminate the synchronous component in the control current and realize the autobalancing of vibration displacement. Computational complexity compared with conventional control strategies is further investigated. Moreover, the velocity sensor is no longer needed in the proposed control strategy. The simulation and experimental results of the AMB system show that the synchronous component of the control current and the amplitude of the rotor vibration displacement can be significantly reduced.

Research on overvoltage suppression of three‑level converter based on two-three phase mixed conduction mode

Sifeng Zhao,Caiyong Ye,Shanming Wan 전력전자학회 2024 JOURNAL OF POWER ELECTRONICS Vol.24 No.2

For medium and high voltage drives, the three-level converter has better technical and economical performances than the two-level converter. Permanent magnetic brushless DC (BLDC) motors are widely used due to their simple control, lowresolution requirement of the position sensor, and high power density. The power switching devices in the non-conducting phase are blocked all the time when the two-phase conduction mode is used. Serious overvoltage of the inner power switching device occurs, which triggers the overvoltage alarm or causes system failure. In this paper, the overvoltage mechanism of the inner power switching device is analyzed in detail, and a mixed conduction mode containing a three-phase conduction mode in the first stage and a two-phase conduction mode in the second stage is proposed. The speed threshold for the switching of the two control modes is designed to suppress overvoltage in the starting stage and to reduce the loss of the converter at high speeds. The effectiveness of the overvoltage suppression and system safety of the proposed mixed conduction mode are verified by simulations and experiments.

Simulation of Active Compensated Pulsed Alternator with a Laser Flashlamp Load Based on Simplified Model

Yuan, Pei,Yu, Kexun,Ye, Caiyong,Ren, Zhang'ao Journal of International Conference on Electrical 2012 Journal of international Conference on Electrical Vol.1 No.4

This paper presents a nontraditional laser power system in which an active compensated pulsed alternator (ACPA) drives a flashlamp directly without the use of capacitor groups. As a result, the volume of the laser system is decreased because of the high energy density of the ACPA. However, the difficulty in matching the output of the alternator with the laser flashlamp is a significant issue and needs to be well analyzed. In order to solve this problem, based on the theory for ACPA, the authors propose a simplified model for the system of ACPA with flashlamp load by the way of circuit simulation. The simulation results preliminarily illuminate how the performance of the ACPA laser power system is affected. Meanwhile, the simulation results can also supply a consultation for future ACPA laser power system design and control.

Mathematical Model Relating Dynamic Dewatering Process of Fresh Mortar and Its Composition

Tao Ji,Xiaoying Zhang,Xiong Zhang,Yong-juan Zhang,Caiyong Gu,Weihua Li 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.2

The composition and properties of raw materials have significant influences on water retention capacity of mortar, but the basic origin remains unclear. To clarify the movement of water inside mortar and the mechanism of water retention, the dynamic dewatering experiment was designed and the mathematical model describing the dewatering process of mortar was set up in this work. The model proved to be effective according to the results from dynamic dewatering experiment. The study demonstrated that capillary gradual contraction model is suitable for describing early dewatering process of fresh mortar and the parameters in the dewatering equation are relevant to the properties of raw materials, which certify that the model is of physical significance. Moreover, influencing factors such as water properties and binding materials properties were obtained and their relationship with water retention of mortar were discussed theoretically based on capillary gradual contraction model.