溫胞散이 생쥐의 排卵과 受精卵 生産率 및 發達率에 미치는 影響

宋炳基,李京燮,李鎭武 慶熙大學校韓醫科大學韓醫學硏究所 1999 慶熙韓醫大論文集 Vol.22 No.1

In order to study the effect or Onposan applied to sterile women, a series of expriments were conducted on the mice regarding the in vitro developmental effect of mouse embryos of the medium of Onposan, the ovulation of normal ovum, the production rate of embryos in mice, the in vitro developmental effect of mouse embryos, the level of serum of LH, FSH, Estradiol 17-β, Progesterone in mice, after administered the drug orally. The results were as follows. 1. The culture medium of the drug was not significantly the in vitro developmental effect of mouse embryos. 2. The ovulation or normal ovum was significantly increased after continuous the drug administered. 3. The production rate of embryos in mice was significantly increased after the drug administered. 4. The in vitro developmental effect of mouse embryos was significantly increased after the drug administered. 5. The levels of serum LH and FSH in mice were not significantly increased after the drug administered. 6. The levels of serum Estradiol 17-β in mice was few increased in a very small amount, but was not significantly. 7. The levels of serum Progesterone in mice was significantly increased after the drug administered.

귀출파징탕藥鍼의 抗血栓, 抗炎症, 鎭通 및 抗酸化效果

宋炳基,李京燮,宋銀姬 慶熙大學校韓醫科大學韓醫學硏究所 1999 慶熙韓醫大論文集 Vol.22 No.1

This study was projected to investigate the effects of Kwuichulpajing-tang aqua-acupuncture solution manufactured by water-alcohol method. In vivo, to observe the anticoagulative, antiinflammatory and analgesic effects of Kwuichulpajing-tang aqua-acupuncture at Kwanwon(CV4), the experimental animals were divided into a normal, control and two experimental groups. Each of them had 6 animals. As the normal group was untreated, the control group was injected with normal saline(0.1㎖/100g) at Kwanwon(CV4) s.c., and then injected with induction medicine. One of the experimental groups (Sample I group) was injected with 25.0 ㎎/㎖ Kwuichulpajing-tang aqua-acupuncture solution(0.1㎖/100g) at Kwanwon (CV4) s.c., and then injected with induction medicine. The other experimental group(SampleⅡgroup) was injected with 50.0 ㎎/㎖ Kwuichulpajing-tang aqua-acupuncture solution (0.1㎖/100g) at Kwanwon(CV4) s.c., and then injected with induction medicine. The results of the experimental groups were compared with that of the control group. In vitro, the effects of Kwuichulpajing-tang aqua-acupuncture solution on hyaluronidase activity, hydrolysis of casein by trypsin, heat-induced hemolysis and the antioxidative effect were observed. The results were as follows : 1. The effects of Kwuichulpajing-tang aqua-acupuncture at Kwanwon on the intravascular coagulation in rats induced by endotoxin(0.5㎎/㎏) i.v. were that the blood platelet and white blood cell counts were significantly increased in both experimental groups. the blood fibrinogen content was significantly increased in SampleⅡgroup and the prothrombin, the activated partial thromboplastin and thrombin times were significantly shortened in both experimental groups. 2. The carrageenin-induced mice paw edema was significantly decreased in both experimental groups. 3. The histamine-induced mice paw edema was significantly decreased in both experimental groups. 4. The histamine-induced vascular permeability was significantly decreased in SampleⅡgroup. 5. The writhing syndrome induced by acetic acid was significantly decreased in SampleⅡgroup. 6. The response time of mice on 70℃ heat plate was significantly prolonged in SampleⅡgroup. 7. The hypnotic duration induced by pentobarbital-Na was significantly increased in both experimental groups. 8. Kwuichulpajing-tang aqua-acupuncture solution had antioxidative effect and inhibited hyaluronidase activity, trypsin activity on hydrolysis of casein and heat-induced hemolysis in vitro.

膠艾四物湯 加三七根이 止血作用에 미치는 影響

金日文,宋炳基 慶熙大學校 1983 論文集 Vol.12 No.-

In order to observe the hemostatic effect of kyoaesamultang added Pseudoginseng Radix, the plasma prothrombin time and the blood coagulation time were measured after the oral administration of the prescriptions, such as the extract powder of kyoaesamultang: the mixture of individual extract powder of Kyoaesamultang: the extract powder of Kyoaesamultang added Pseudoginseng Radix and the mixture of individual extract powder of Kyoaesamultang added Pseudoginseng Radix, to mice and rat. The following results were obtained. 1. With the adminstration of Kyoaesamultang, the plasma prothrombin time was considered to be significant at the trial ground of the extract powder (S-Ⅰ_1) and the mixture of individual extract powder (S-Ⅰ_2) 2. With the administration of Kyoaesamultang added Pseudoginseng Radix, the plasma prothrombin time was considered to be high significant at the trial group of the extract powder (S-Ⅱ_1) and the mixture of individual extract powder (S-Ⅱ_2) 3. At the condition of hypoprothrombinemia, the plasma prothrombin time of the trial group of the extract powder of Kyoaesamultang added Pseudoginseng Radix (S-Ⅱ_1) was showed faster than the control group, and was significant from 24 hours. 4. At the condition of hypoprothrombinemia, the blood coagulation time of the trial group the extract powder of Kyoaesamultang (S-Ⅰ_1) was not appeared significant. But at the trial group of the extract powder (S-Ⅱ_1) and the mixture of individual extract powder of Kyoaesamultang added Pseudoginseng Radix (S-Ⅱ_2), the blood coagulation time was considered to be high significant.

勝金丹이 卵巢機能에 미치는 影響

姜明孜,宋炳基 慶熙大學校 1985 論文集 Vol.14 No.-

Seungkumdan has reputed to be effective for the remedy of sterility in order to study the effects of Seungkumdan on ovrian function, a series of experiments were conducted on the pregnancy and ovulation in rats and on the progesterone level and serum estradiol-17β level in female adult rabbits and then the following results were obtained. 1. The antiabortive effect of the drug was significantly increased in proportion to the amount administered. 2, The level of serum progesterone in pseudopregnant rabbits was significantly increased. 3. The number of ovum during ovulation in drug administered rate was increased in all groups when the drug was given at 14:30 and 15:30 and increased only in the case of the group which is administered the extracted powder when it was given at 21:30 and 22:30. PGS_2α 4. After administering Seungkumdan, the effect on antiovulation in PGS_2α treated animal showed the increasing number of ovum. 5. The level of estradiol - 17β in serum of pseudopregnant rabbit showed the decreasing tendency at the early stage of administration, but afterwards showed the remarkably increasing tendency. According to the above results, it can be concluded that Seungkumdan takes effect on the functional sterility and that the extracted powder of Seungkumdan has a better effect than the mixture of each extracted powder of Seungkumdan in the experimental animals.

한방처방구성원리의 과학적 해석연구(Ⅱ) : 상한론 처방의 통계적 분석연구 Statistical Analysis of Sang-Han Prescriptions

김남재,김동현,장준복,송병기 慶熙大學校 1997 論文集 Vol.26 No.-

This study was made to objectify the principle of oriental medical prescriptions so that we could obtain the principle and the formulation of them. For that purpose, we analysed the formula and rule of 113 prescriptions recorded in 「ShanghanLun」, which have a few of components, and re widely used in clinical from ancient times to these days. At first we classified those prescriptions by their effect. Then we re-classified the herbs of the effect groups into four natures(cold, hot, warm and cool) and five kinds of flavors(wour, bitter, sweet, acrid and salty). And we classified the herbs into three grades -superior, medium, and inferior which were used in Shen Nong's Herbal Classic. By these means, we statistically evaluated the prescriptions recorded in 「ShanghanLun」based on the Four Regular Components(Monarch, Minister, Assistance and Laborer) According to the frequecy of the herbs used on the 108 prescriptions recorded in 「ShanghanLun」there are many concidences with the herbs used in these days. According to the effects, formulation of prescriptions and the effects have very colse relationship, and it might be related with the diseases on those days. The formulation of the prescriptions was carefully made by the for natures and five kinds of flavors. On the study of the three grades of Shen Nong's Herbal classic the superior and medium herbs mostly used, and the inferior herbs were very seldom used.

姙娠 및 分娩에 미치는 達生散의 效果에 關한 硏究

李恩美,李京燮,宋炳基 慶熙大學校 1986 論文集 Vol.15 No.-

In order to study the effects of Dalsaengsan, reputed to be the effective for the remedy and treatment of hard labor, experiments regarding the antiabortive effect of Dalsaengsan on the pregnant rats and the effect of it on the uterine smooth muscle strips of nonpregnant rats were carried out. The results were as follows 1. The drug administered on the uterine muscle strips of nonpregnant rats caused the effect of uterine relaxation in proportion to concentration of the drug. 2. The drug administered on the uterine contraction induced by oxytocin or prostaglandin F_(2α) of nonpregnant rats caused the effect of uterine relaxation in proportion to concentration of the drug. 3. The drug administered on the antiabortive effect when the dosage of the drug was 640 ㎎/㎏. 4. The effect of extracted powder and mixture of each extracted powder was nearly the same on the relaxation effect of the drug on the uterine muscle strips of the nonpregnant rats, but the effect of the former was better than that of the latter on the antiabortive effect.

陰戶病의 外用藥으로 應用되는 蛇床子, 百部根, 枯白?, 硼砂의 抗菌과 消炎效果

金亨俊,李京燮,宋炳基 경희대학교 1994 慶熙醫學 Vol.10 No.3-4

애엽, 지각, 아교주 및 목향의 투여가 임신중 생쥐의 생식능력에 미치는 효과

정형민,차수경,임정묵,신태은,박찬,장준복,이경섭,송병기 경희대학교 2000 慶熙醫學 Vol.16 No.2

Effects of Daeyoung-Jun on Sex Hormone Activity and Bone Metabolism in Ovariectomized Rats

Song, Byoung-key,Jang, Jun-bock INSTITUTE OF ORIENTAL MEDICINE KYUNG-HEE UNIVERSIT 1998 JOURNAL OF ORIENTAL MEDICINE Vol.3 No.1

This study was performed to investigate the effect of Daeyoung-Jun in oriental medicine on climacteric disturbance. The experimental model of menopause was induced by bilateral ovariectomy in rats. These were administered orally with liquid extract Daeyoung-Jun 1.6g/kg for 30 days before ovariectomy (Sample A group), for 30 days after ovariectomy(Sample B group) and after an additional 30 days of ovariectomy (Sample C group). The level of serum FSH, serum LH, serum estradiol, serum progesterone, total ash weight, calcium in the tibia, serum osteocalcin, serum alkaline phosphatase activity was measured. The differences in these levels were obtained at 40, 50 and 60 days after ovariectomy. From this study we could recognize that Daeyoung-Jun has the effect of controling the hypothalamus-pituitary gland-ovary axis, so it can improve sex hormone changes and the imbalance of bone metabolism. We came to find out that the most appropriate dosing time was athe Sample C group. Therefore we expect that this kind of oriental medical treatment can be used for osteoporosis.

Scientific Analysis of Fundamental Formulation Theory of Traditional Herbal Medicinal Polyprescription(Ⅰ) : Effect of Herbal Medicines on the Absorption and Metabolism of Glcyrthizin of Glycyrrhizae Radix

Park,Byoung-mun,Bae,Eun-ah,Lee,Sung-won,Jang,Jun-bock,Song,Byoung-key,Kim,Dong-hyun,Kim,Nam-jae INSTITUTE OF ORIENTAL MEDICINE KYUNG-HEE UNIVERSIT 1996 JOURNAL OF ORIENTAL MEDICINE Vol.1 No.1

Traditional medicinal polyprescriptions were formulated based upon the conditions of the patients and the medicinal material properties. The fundamental theory which formulate traditional medicinal polyprescriptions lies in the relationships of the Four Regular Component; Monarch(君藥), Minster(臣藥), Assistant(佐藥), Laborer(使藥). We investigated the fundamental formulation theory of the prescription by experimenting the effect on the absorption of glycyrrhizin(GL) contained in Glycyrrhizae Radix(GR) in rat small intestines in situ and the metabolism of GL by intestinal bacteria and the effects of herbal drug conbinations on the metabolism of GL. The absorption rate of GLL was only 19.9% at 1hr after incubation, but the GR was 7.3%. The low absorption rate was increased through a combination with Cinnamomi Cortex (CC), Paeoniae Radix (PR) and Holen (HO) with GR. Among them, CC was best, followed by PR and HO. However, Ginseng Radix (GiR) was not effective. Human intestinal bacteria was cultured in GAM containting GIR, CC, PR or HO with GR and assayed for the metabolite, glycyrrheticacid (GA) of GL. CC and GIR induced the biotransformation of GL to GA, but PR and HO inhibited it. By using rat intestinal bacteria, CC and GIR induced the biotransformation of GL of GR to GA, but PR and HO inhibited it.