C형 간염 바이러스의 세포배양(Ⅱ)

황태욱,유병제 대구대학교 기초과학연구소 2000 基礎科學硏究 Vol.17 No.2

In this research, It was investigated to increase the infectious efficacy of HCV to culture cells, and the efficacy of HCV cell culture system. To increase the infectious efficacy to human cells, The human hepatoma cell lines (Huh_(7)) was used as the culture cells to test the HCV infection. The whole patient serum, the high density portion, and the low density portion were added in the culture media of Huh_(7) cells as the infectious sources. In the high density portion, the HCV genomes were not detected in the media and cells of all days except in the 2 day culture medium, but in the low density portion, the HCV genomes were detected in the media and cells of all days. It was showed that HCV partcles of the high density portion were not infectious to Huh_(7) cells, but HCV particles of the low density portion were infectious to Huh_(7) cells. Therefore, it was concluded that the antibody bound HCV was not infectious to Huh_(7) cells, but the antibody free HCV was infectious to Huh_(7) cells.

構造用鋼의 彈塑性破壞靭性 JIC 評價에 關한 硏究 (Ⅰ)

安秉旭 大田工業大學 1988 한밭대학교 논문집 Vol.5 No.2

J-integral estimation method, such as, R-curve method, Stretched Zone Width method, and Unloading Compliance method are studied and shown to be useful. The following results for 0.41% carbon steel are obtained. (1) Elasto-plastic toughness JIc estimation by R-curve method are overestimated than that by SZW method, and that by unloading compliance method is estimated a middle-value of them. (2) Unloading Compliance method, witch is a single specimen method, is economical and reliable to the identification of fracture mechanism. (3) Stably growing ductile fracture in crack propagation curve and the co-extistence of cleaveage fracture and stable crack is observed. (4) In identification of ductile fracture, material can be more precisely evaluated by the metallographic method of fractography, and microscophic structure with the time history study of fracture process.

Sucrose density gradient에 의해 분리된 C형 간염 바이러스의 특성 분석(Ⅱ)

황태욱,유병제 大邱大學校附設 基礎科學硏究所 2000 基礎科學硏究 Vol.16 No.2

To study the buoyant density of HCV, the human patient sera was tested with both anti-HCV ELISA and RT-PCR for the detection of HCV. The patient sera, positive at two tests, were used for further experiments. After ultracentrifugation of 20-60% sucrose linear density gradient, the centrifugal medium containing HCV was fractionated by 280 ul from the bottom. The buoyant densities of all fractions were determined, and the HCV in all fractions were checked by the RT-PCR. There was the room to separate the fractions to two major parts containing HCV. One was the high density part (1.146 g/ml ∼ 1.207 g(ml), and the other was the low density part (1.089 glml ∼ 1.108 glml).

監査人 質과 去來量反應에 관한 硏究

鄭秉旭 대진대학교 1995 大眞論叢 Vol.3 No.-

By examining trading volumes of the stocks for 30 days after their initial public offerings, this paper investigate the relationship between auditor choice and trading activities and thereupon makes an inference on the auditor quality issue. The results show that the trading volume of IPO stocks on the first day of trading is significantly larger for High-Quality auditor clients than for Low-Quality auditor ones, but this relationship is reversed after 10 trading dates.

Dimethylphthalate와 Monoethanolamine의 에스테르 交換反應에 關한 硏究

孫秉淸,鄭舜旭 弘益大學校 1983 弘大論叢 Vol.15 No.2

The transesterification reaction between dimethyphthalate and monoethanolamine was kinetically investigated in the presence of various metal acetate catalyst at 110℃. The reaction was followed by the measurement of distilled methanol from the micro measuring cylinder The transeterification reaction was first-order kinetics with respect to the concentration of dimethylphthalate, monoethanolamine and catalyst. From the examination of relationship between rate constant and reciprocal absolute temperature, the activation energy has been caculated as 9.843 Kcal with cadmium acetate catalyst, 12.30 Kcal without catalyst. The highest reactives were found for electronegativity values between 1.4~1.7.

婚姻適齡 改正論

崔柄煜 梨花女子大學校 法學硏究所 1998 法學論集 Vol.3 No.1

Sucrose density gradient에 의해 분리된 C형 간염 바이러스의 특성 분석(Ⅲ)

황태욱,유병제 大邱大學校附設 基礎科學硏究所 2000 基礎科學硏究 Vol.16 No.2

To study the buoyant density of HCV, the human patient sera was tested with both anti-HCV ELISA and RT-PCR for the detection of HCV. The patient sera, positive at two tests, were used for further experiments. After ultracentrifugation of 20-60% sucrose linear density gradient, the centrifugal medium containing HCV was fractionated by 280 ul from the bottom. The buoyant densities of all fractions were determined, and the HCV in all fractions were checked by the RT-PCR. There was the room to separate the fractions to two major parts containing HCV. One was the high density part (1.131 g/ml - 1.240 g/ml), and the other was the low density part (1.103 g/ml - 1.122 g/ml). There was not the high density part in the serum treated with PAS for removing of antibody bound HCV. Therefore, it was concluded that the HCV in high density portion were the antibody bound forms, and the HCV in the low density portion were the free forms.

Sucrose density gradient에 의해 분리된 C형 간염 바이러스의 특징 분석(Ⅰ)

황태욱,유병제 대구대학교 기초과학연구소 2000 基礎科學硏究 Vol.16 No.2

To study the buoyant density of HCV, the human patient sera was tested with both anti-HCV ELISA and RT-PCR for the detection of HCV. The patient sera, positive at two tests, were used for further experiments. After ultracentrifugation of 20-60% sucrose linear density gradient, the centrifugal medium containing HCV was fractionated by 280 ul from the bottom. The buoyant densities of all fractions were determined, and the HCV in all fractions were checked by the RT-PCR. There was the room to separate the fractions to two major parts containing HCV. One was the high density part (1.161 g/ml - 1.192 g/ml), and the other was the low density part (1.085 g/ml - 1.117 g/ml).

C형 간염 바이러스의 세포배양(Ⅰ)

황태욱,유병제 大邱大學校附設 基礎科學硏究所 2000 基礎科學硏究 Vol.17 No.2

In this research, It was investigated to increase the infectious efficacy of HCV to culture cells, and the efficacy of HCV cell culture system. To increase the infectious efficacy to human cells, The human hepatoma cell lines (Huh_(7),) was used as the culture cells to test the HCV infection. The whole patient serum, the high density portion, and the low density portion were added in the culture media of human hepatoma (Huh_(7)) cells as the infectious sources. It was showed that HCV partcles of the high density portion were not infectious to Huh_(7) cells, but HCV particles of the low density portion were infectious to Huh_(7) cells. Therefore, it was concluded that the antibody bound HCV was not infectious to Huh_(7) cells, but the antibody free HCV was infectious to Huh_(7) cells.

성인 음낭수종의 치료로서 Window Operation의 효용성

서병욱,박영호,이남규,전윤수,김민의,김영호,송윤섭,구자현 순천향의학연구소 1999 Journal of Soonchunhyang Medical Science Vol.5 No.1

We intended to determine the usefulness of window operation for adult hydrocele repair. We reviewed medical records of 132 patients from January 1995 to June 1998 with primary hydrocele and evaluated complications and recurrence rates of eversion and/or excision, plication, and internal drainage technique. Recurrence rate was highest in internal drainage technique as 85.2%, and those of eversion and/or excision and plication technique were 1.3% and 4%, respectively. Complication rate was highest in eversion and/or excision technique, and lowest in internal drainage technique. Although complication rate was lowest in window operation, this operation could not be successful due to high recurrence, and we suggest that this operation should be abandoned for hydrocele repair.