藥鍼用蜂毒液이 黑色腫細胞에 미치는 抗癌效果에 對한 分子生物學的 硏究

朴贊烈,南相水,金昌煥,李栽東,姜成吉,李潤浩,安秉哲 경희대학교 동서의학연구소 2001 東西醫學硏究所 論文集 Vol.2000 No.-

To study anti-cancer effect and molecular biological mechanism of bee venom for aqua-acupuncture, the effects of bee venom on cell viability, apoptosis, and cell cycle were analyzed using MTT assay, tryphan blue assay, [3H]thymidnine release assay, flow cytometric analysis, activity of caspase-3 protease activity assay, and immunocytometric analysis of PCNA. To explore whether anti-cancer effects of bee venom are associated with the transcriptional control of gene expression, quantitative RT-PCR analysis of apoptosis- and cell cycle-related genes was performed. The obtained results are summarized as follows: 1.The MTT assay demonstrated that cell viability was decreased by bee venom in a dose-dependant manner. 2.Significant induction of apoptosis was identified using tryphan blue assay. [³H]thymidine release assay, and flow cytometric analysis of sub G₁fraction. 3.In analysis of caspase-3 protease activity, the activity had increased significantly, in a dose-dependant manner. 4.Quantitative RT-PCR analysis of the apoptosis-related genes showed that Bcl-2 and Bcl-X□ were down-regulated whereas Bax was up-regulated by bee venom treatment. 5.In flow cytometric analysis of cell cycle and immunocytometric analysis of PCNA expression, cell numbers of G₁phase was increased by a dose-dependant manner. 6.In quantitative RT-PCR analysis of the cell cycle-related genes, p21, p27, and p57 were increased, while Cyclin D1, CDK4, c-Myc, c-Fos, and Histone H3 were decreased. In contrast, there were no remarkable changes in expression levels of CDC2 and c-Jun.

小農의 農業機械化에 관한 硏究

趙炳贊 동국대학교 1978 論文集 Vol.17 No.-

The limitation of the individual farmland with 3 hectares by the Farmland Act has been discussed as a main obstacle for the farm-mechanization in Korea by many experts and scholars. They insist that the limitation should be removed compeletely or relaxed to permit the individual possession of 4 to 5 hectares, a break-even point for the mechanization. Their idea has a refutation on this major, but it might be wrong practically and empirically as the following reasons. 1. The average size of farmland per farm household is 0.95 hectares in Korea. 67.4 percents of farmers possess less than 1 hectares, while the percentage of those who owned 3 hectares or more is only 1.5%. 2. The farm-mechanization was successfully achieved in Japan where the size of the individual farmlands are as small as in Korea. 3. A very few farmers who own more than 3 hectares and some farmers who own plow-machines are unable to mechanize their farming completely because their farms are divided into many smaller pieces and scattered around. Average number of pieces of the individual farmland in Pyongtaek-gun, Kyonggi-do is 13.1. Now I would like to suggest that the desiresble and accessible direction of farm-mechanization in Korea would be to organize the farmers into units for use of farm-machine. The unit is possible with the natural villages or groups of farmers within a village. In conclusion, it is believed that the mechanization of farming in Korea should be studied and considered on the basis of the structure of small farms.

Cyan Coupler의 합성과 발색현상에 관한 연구

金榮燦,鄭舜旭,孫秉淸 弘益大學校 科學技術硏究所 1995 科學技術硏究論文集 Vol.6 No.-

In this paper, cyan coupler was prepared by the reaction of 2-amino-4.6-dichloro-5-me-thylphenol with 2,4-ditirtiarypentyl-phenoxy-butyroyl chloride in the presence of pyridine. The product was identified by using various analytical tools such as melting point. Elemental analyzer. IR spectrophotometer. UV-Vis spectrophotometer Mass Spectrometer. The reaction of cyan coupler with CD-3(color development agent) was shown cyan color.

카올린으로부터 액상침전법에 의한 미립 알루미나 합성

박희찬,강효경,김병찬,박성수 釜山大學校生産技術硏究所 1999 生産技術硏究所論文集 Vol.56 No.-

카올린으로부터 미립 일루미나 분말을 합성하였다. 황산용액을 이용하여 액상침전법으로 알루미나를 추출하였다. 알루미나 추출에 미치는 황산농도, 반응온도 및 반응시간의 영향을 조사하였다. 이 과정에서 수화 황산알루미늄이 용출액으로부터 석출되었다. 이때 석출제로는 에탄올을 사용하였다. 이 석출물을 가영하여 미립 알루미나 분말을 얻을 수 있었다. DTA, SEM, TEM 및 XRD를 이용하여 분말의 특성을 조사하였다. Fine alumina was synthesized from kaolin. The alumina was prepared by liquid phase precipitation method using H?SO? solution. The effects of acid concentration, reaction temperature and reaction time on alumina extraction were investigated. Using an ethanol as precipitating agent, hydrated aluminum sulfate was precipitated from leach liquor. Fine alumina powder was obtained by calcining the precipitate. DTA, SEM, TEM and XRD were employed to characterize the powder.

「南原古詞」의 構成

李秉讚 대진대학교 1996 大眞論叢 Vol.4 No.-

This study is the work that I have researched the plot on <NAMWONGOSA>, one of the representative another volumes of the <CHUNHYANGJUN>. The characteristic of this novel is the longest story among the <CHUNHYANGJUN> that were written before 20th century and is composed of more than about one hundred thousand words. It's plot is largely divided into the former part and the latter. The former part is the first meeting, the true love, and the parting course. The latter is expressed with the course in which the two characters must overcome the trial of their loves and relieve the pain each other. They are destined to meet the desperate love in the former part, but as a resolt they have to achieve their profound love with their happy ending.

선인장 양심실 보조장치의 설계 및 임상적용을 위한 평가

민병구,박찬영,최재순,이혁수,황창모,김삼성,윤걸중,김종원,선경,이경갑,정종태,김원곤 제주대학교 인공심장이식연구소 2001 인공심장 연구 Vol.2 No.1

현재 개발되고 있는 맥동형 이식형 인공심장은 완전이식형 인공심장과 좌심실보조장치 뿐이다. 좌심실보조장치를 장착한 환자의 10~15%가 우심실의 보조를 필요로하고 완전이식형 인공심장의 장착을 위해서는 자연심장을 제거해야하는 상황에서 이식형 양심실보조장치에 대한 필요성이 대두되고 있다. 본 연구진은 완전이식형 인공심장으로 개발된 한국형 인공심장을 개선하여 양심 실 보조장치를 개발하였다. 양심실보조장치는 이동형 작동기식 미케니즘을 이용하고 있으며 에너지 변환장치와 감속기로 구성된 작동기와 혈액주머니, 그리고 내장형 제어기로 구성되어 있다. 선인장 펌프로 명명된 KAH350은 캐뉼라의 연결을 위한 커넥터를 포함하여 길이 177mm, 폭 164mm, 높이 67 이고 무게 780g이며 최대 심박출량은 5L/min 이다. 양심실보조장치로 개발된 선인장펌프는 좌심실보조장치로도 응용이 가능하다. 이식적합성과 생체적합성을 평가하기 위하여 좌심실보조장치로 5회, 양싱실보조장치로 6회의 동물실험을 수행하였다. 각각 최장 28일간 생존하였으며 11회의 동물실험중 장치의 결함에 의한 것이 3회 있었는데 이들은 모두 전자장치의 결함과 방수처리문제였다. 현재 이식적합성과 일박출량을 향상시킨 KAH400모델이 개발중이다. The types of pulsatile implantable artificial hearts that are on the way of development and have been developed are totally implantable artificial heart and implantable left ventricular assist device (LVAD). Approximately 10% to 15% of all patients Implanted with wearable VADs have required right heart support with another device. And it is reluctant to patient who should remove his or her own heart to be implanted with total artificial heart. These situations drive the development of implantable bi-ventricular assist device (BVAD). The Korean BVAD was develophed by modifying the moving actuator type Korean artificial heart. This electro-mechanical BVAD comprises actuator including energy converter and reduction gear train, blood sacs, and internal motor and energy controllers. The KAH350 which is named as 'Cactus Pump' is 177mm in length, 164mm in width, and 67mm in thickness including connectors and nuts. The weight and maximum cardiac output of Cactus Pump is 780g and 5L/min, respectively. The Cactus Pump that was developed as BVAD could be used as LVAD by attaching compliance caps on the 2 ports of one ventricle. The animal experiments were undergone 5 times for LVAD, and 6 times for BVAD. The best records were 28 days survival in both applications. There were 3 times of device failure and they were all associated with electrical connection and hermetic sealing. The KAH400 that has improved anatomical fitting characterisitcs and stroke volume is on development.

豚 Pepsin의 凝乳特性에 關한 硏究

尙炳贊 충남대학교 대학원 1975 論文集 Vol.5 No.-

These studies were carried out to investigate the properties of swine pepsin as milk-clotting enzyme and its utilization in the process of cheese making. The results were summarized as follows. 1) The optimum temperature for milk-clotting activity of swine pepsin was appeard at 40∼45℃, and the swine pepsin was completly inactivated at higher than 60℃. 2) The optimum pH for milk-clotting activity of swine pepsin was pH 4.0∼5.0, and the swine pepsin was comppletly inactivated at higher than pH 6.0 3) When the pepsin solution was treated by heating at 30∼70℃ for 10 minutes, pepsin activity was decreased seriously at 50℃, and it was completly inactivated at 60℃. 4) The higher keeping temperature and longer storaging period of the pepsin solution was showed the less milk-clotting activity. 5) Sodium propionate and Potassium sorbate were the most effective antiseptics for the preservation of the pepsin solution. 6) When the pepsin solution was exposed sunlight for 9hours, 26.5% of the enzyme activity was decreased. 7) Various animal serums were decreased the activity of the swine pepsin, especially swine serum of them was showed the most inactivity effect. 8) When 0.1㎖ of soy bean extract was added to 0.2㎖ of pepsin solution, 83% of pepsin activity was decreased. 9) Ammonium sulfate of the various saturated salt solutions was showed the most inactivating effect, but heavy metal salts were showed the activating effects. 10) The higher concentration of CaCl₂ in milk showed the higher milk-clotting activity of the pepsin. When 0.02% of CaCl₂ was added to milk, 43.4% of the pepsin activity was increased. 11) The curd weight per milk weight obtained 10.80% from swine pepsin, and 11.40% from rennet. But the difference between these was no significant.

寫眞 乳劑用 硬膜劑 合成과 應用

金榮燦,孫秉淸 弘益大學校 科學技術硏究所 1994 科學技術硏究論文集 Vol.5 No.-

대기 중 휘발성 유기화합물의 Advanced Technology에 대한 실험비교

최금찬,안병주,김석택,김찬훈,정창훈,서정민,유수영 동아대학교 환경문제연구소 2000 硏究報告 Vol.23 No.1

Nowaday, Advanced technology has been applied to organic destruction technologies that are alternatives to incineration. Plasma, photolysis, and Photocatalytic oxidation processes are an emerging advanced technologies for the abatement of volatile organic compounds(VOCs) in atmospheric pressure air streams, so we has been used these technologies for destruction of VOCs. TCE, Benzene, Toluene, and Xylene were selected as objective materials. We have been studied each process and found many types of reaction parameters which effect on treatment efficiency. So combination processes, which are plasma-photocatalytic oxidation process and photolysis-photocatalytic oxidation process, have been used to increase destruction efficiency. Analysis of above materials and byproducts was carried out by GC-FID.

離農現象과 農家 勞動利用構造에 關한 調査硏究

趙炳贊 동국대학교 농림과학연구소 1971 農林科學 論文集 Vol.4 No.-

As generally known, the rural labor force in Korea is characterized by 1) over-employment in the agricultural sector, 2) shortage of manpower in the farming season even under over-employment condition and) lower coefficient of labor utilization. These factors suggest that the agricultural labor in Korea is still in a premordern state. It is to be noticed that in the recent years our agricultural labor force has shown a tendency to decrease due to ever-increasing out-flow to urban communities, causing a severe shortage of manpower in the farming season which affects agricultural productivity in Korea. Keeping the above problems in mind, the author surveyed the rural labor force on a village in the suburb of Seoul, in order to analyze and investigate the aspects of leaving farms and structure of labor utilization. Village surveyed; Village of Yadang-dong, Yadang-ri, Kyoha-myon, Paju-kun, Kyunggi-do. Method of survey; Complete survey of 50 households which comprise the village. Dates of survey; August 1-31, 1970. The results obtained from this survey are summarized as follows; 1. Phenomenon of leaving farms (1) The out-flow of these villagers to urban communities was observed, in general, among the group of the high teens and the first half of forties. The leaving rate is higher in young group. Accordingly, the manpower of this village was old aged; i.e., 34.7% of the age group of 15-24, 32.1% of 25-34 and 22.6% of 35-44 left the village during a period of Jan. 1965-Aug. 1970. (2) The leaving rate is markedly high in the group of higher educational background; i.e.,3.4% of uneducated or under primary school graduates, 20.1% of primary school graduates, 21.7% of secondary school graduates, 42.9% of high school graduates and 88.9% of college or university graduates. (3) In order to analyze the effect of the scale of cultivated area and farm household income on the leaving farms, the correlation between the two factors was investgated, The resultes revealed very low degree of correlation between them. 2. Utilization structure of labor The number of working days per capita in a year and rate of labor consumption (utilization) was 164.0 days and 54.7% for male, 113.7 days and 37.9% for female, and the average of the both sexes was 137.9 days and 46.0%(The percentage is based on 300 working days in a year - 25 days in a month). Such low coefficient of labor utilization may be attributed to the following factors; (1) The seasonal change in the demand of manpower; because of simple cropping method in our country, which depends entirely upon seasonal factors. (2) The fact that most of youth group in the village tend to avoid farming work and to find other jobs because of their inferiority complex with agriculture. Most of them refuse to engage in agriculture even if in the unemployment status. (3) The low participating ratio of labors of both sexes in non-farming work in the farming community; because they are rarely given the opportunity to find and to be trained for a proper job in and around their farm village. 3. Factors determining the labor utilization Upon analyzing, as the determining factors of individual labor utilization, 1) age of laborer, 2) scale of cultivated land, 3) scale of farm household income, it is revealed that the most significant factor is the age of laborer. The age means working capacity of individual farmer and his position or responsibility in the household; i.o., dependent or not. 4. Conclusion (1) The out-flow of the young and educated from this village resulted in; 1) that the average age of the remaining labor is high, 2) that the educational level of the remaining labor is low, causing intellectual vacuum in the village. (2) Utilization of labor The rate of labor utilization in this village is very low, mainly from; 1) the long farmer's slack season caused by the mono-cropping system which depends upon entirely seasonal factor. 2) the difficulties to find proper work (especially non-farming)during the farming slack season. 3) traditional family system of Korea with large number of dependents who can and should work, but whose widely recognized unconscious privilege prevents their positive participation in the farming work.