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손연아,김경란,민병미,최돈형 한국초등과학교육학회 2005 초등과학교육 Vol.24 No.2
The purpose of this study was to analyze the professionalism of elementary school teachers and to draw out factors which elementary teachers should try to improve their professionalism for environmental education. For the study, both Quantitative analysis and qualitative methodologies were applied: First, randomly selected 483 elementary teachers from Seoul and Gyeonggi self responded to 5 evaluation areas by Hungerford et al. (1994) to evaluate their environmental education professionalism through the use of five point Likert scale. Second, 2 elementary teachers' environmental classes were observed for the purpose of analyzing strengths and weaknesses in classroom instruction, and then the results of self-evaluation on environmental education professionalism and teacher professionalism as shown in the classes were compared. It was found that 483 elementary teachers including 2 teachers for analyzing classroom instruction who performed a self-evaluation gave themselves mid-scores on the Hungerford et al.'s 5 evaluation areas. However, the result of self-evaluation did not correspond with the result of actual class observation and teacher interview. That is, the actual environmental classes conducted by 2 teachers did not seemed to reflect the evaluation areas which were the foundation of the study: the conceptual awareness, the investigation & evaluation, environmental action skills, and educational application. The results of this study can be used as the basis reference to develop teaching-learning program and in-service programs for professional development of teachers for successful application of environmental education in elementary school classes.
김갑현,박병배,백현정,이준행,백승운,백경란,장기택 대한내과학회 2004 대한내과학회지 Vol.66 No.3
거대세포바이러스 감염은 주로 면역기능이 저하된 환자에서 치명적인 기회감염으로 잘 나타나는 질환이다. 한편 면역 상태가 정상인 사람에서 발생되는 경우는 매우 드물다. 저자들은 심와부 동통과 구토를 주소로 내원한 정상면역의 27세 남자 1예를 보고한다. 상부위내시경상위 점막 결절상과 궤양소견을 보였고, 위 점막 결절과 궤양부위 조직검사상 거대세포바이러스 봉입체의 관찰로 거대세포바이러스 위염을 진단하였다. 최근 감염과 일차감염을 나타내는 거대세포바이러스 Ig M 항체는 양성이었다. Ganciclovir와 proton pump 억제제를 병용하여 치료하였고 증상 호전과 거대세포바이러스 seroconversion을 확인하였다. 저자들은 거대세포바이러스가 정상면역 성인에서 위 궤양을 일으킬 수 있는 경우를 경험하여 보고하는 바이다. Cytomegalovirus (CMV) infections are usually reported in immunocompromised patients. However, it occurs rarely in immunocompetent individuals. A case of CMV associated with stomach ulcers and mucosal nodules is reported in a immunocompetent host. A previously healthy 27-year-old man visited our hospital with a thirty-day history of epigastric pain and a nine-day history of vomiting. The demonstration of intranuclear inclusion bodies in a few gastric glandular epithelial cells contributed to the diagnosis of CMV gastritis. Treatment with a proton pump inhibitor and ganciclovir was successful to mitigate the epigastric pain and vomiting.
들깨 종자의 GA3 발아 증진 효과 및 발아 관련 유전자 발현양상 검정
Byeong Ju Kang,Ji Hye Yoo,Jae Hoo Choi,Chang Heum Kim,Mi Ran Jeon,Eun Soo Seong,Yea Ji Lee,Jae Geun Lee,Chang Yeon Yu 한국약용작물학회 2016 한국약용작물학술대회 발표집 Vol.2016 No.10
Background : Perilla frutescens L. is a bisexual, annual plant belonging to the Labiatae. Dormancy period of Perilla seed usually ranged from 85-200 days. The germination enhancing effects in a reduced seed vigor during the GA3 treatment has been reported. Objectives of the present study was to evaluate the effect of GA3 on germination rate and the expression of germination-related genes expression by using perilla seeds. Methods and Results : Three different cultivars (Saeyeopsil, Okdong) and accession (line 141) of perilla were used in the experiment and treated with GA3 at different concentration (50, 100, 300, 500 μM) for one day at dark condition. Germination test for perilla seed was carried out by using 100 perilla seeds treated with GA3 at 25℃. Rate of germination were evaluated after 10 days and the experiments were repeated three times in similar condition. Samples were collected from each cultivar and accessions for RNA extraction. After cDNA synthesis quantification, templates were subjected to RT-PCR using actin primers. EST blast performed for sequencing the RT-PCR products. Conclusion : Result showed that 100μM GA3 treated seeds of three perilla cultivars and accessions showed the highest germination rate. However, concentration of GA3 over 100 μM and lower than 100 μM resulted into reduced germination rate. Furthermore, expression of germination-related genes from 100 μM treated GA3 was higher compared to untreated sample by using 100 μM GA3 treated sample by using pC12, pJ14 primers.
CONFIRMATION OF THE EXOPLANET AROUND β GEM FROM THE RV OBSERVATIONS USING BOES
Ran, In-Woo,Lee, Byeong-Cheol,Kim, Kang-Min,Mkrtichian, D.E. The Korean Astronomical Society 2008 Journal of The Korean Astronomical Society Vol.41 No.3
To detect exoplanets and study pulsation of K giant stars, we have observed precise RV (radial velocity) of about 55 early K giant (K0 - K4) stars brighter than V = 5 magnitude since 2003 by using BOES, a high resolution Echelle spectrograph attached to the 1.8 m telescope at BOAO (Bohyunsan Optical Astronomy Observatory). We detected periodic RV variation of KO III star $\beta$ Gem (HD 62509) with a period $P\;=\;596.6\;{\pm}\;2.3$ days and a semi-amplitude $K\;=\;44.8\;{\pm}\;0.7\;ms^{-1}$. If we adopt 1.7 $M_{\odot}$ for the mass of $\beta$ Gem, this yields the minimum mass of the companion m sin i = 2.64 $M_{Jupiter}$. Our results agree well with Hatzes et al. (2006) and Reffert et al. (2006), and confirm their discovery of a planetary object around $\beta$ Gem. We also confirmed about 192 minutes short period stellar oscillation found by Hatzes and Zechmeister (2007). This is the first report of exoplanet detection using BOES and demonstrates that the RV observation using BOES is accurate and stable enough to detect exoplanets around bright K giant stars.
Byeong Hwa Jeon,Dong Seon Kim,Seon-Gil Do,Tae Woo Kim,Young Chul Lee,Yu Ran Lee,Myoung Soo Park,Sunga Choi 대한지역사회영양학회 2013 Nutrition Research and Practice Vol.7 No.1
Bamboo leaves (Phyllostachys pubescens Mazel ex J. Houz (Poacea)) have a long history of food and medical applications in Asia, including Japan and Korea. They have been used as a traditional medicine for centuries. We investigated the mechanism of anti-inflammatory activity of a bamboo leaf extract (BLE) on tumor necrosis factor-alpha (TNF-α)-induced monocyte adhesion in human umbilical vein endothelial cells (HUVECs). Exposure of HUVECs to BLE did not inhibit cell viability or cause morphological changes at concentrations ranging from 1 μg/ml to 1 mg/ml. Treatment with 0.1 mg/ml BLE caused 63% inhibition of monocyte adhesion in TNF-α-activated HUVECs, which was associated with 38.4% suppression of vascular cell adhesion molecule-1 expression. Furthermore, TNF-α-induced reactive oxygen species generation was decreased to 47.9% in BLE treated TNF-α-activated HUVECs. BLE (0.05 mg/ml) also caused about 50% inhibition of interleukin-6 secretion from lipopolysaccharide-stimulated monocyte. The results indicate that BLE may be clinically useful as an anti-inflammatory or anti-oxidant for human cardiovascular disease including atherosclerosis.
Effects of GA3 on Seed Germination Rates and Expression of Germination-Related Genes in Perilla
Byeong Ju Kang(강병주),Ji Hye Yoo(유지혜),Jae Hoo Choi(최재후),Chang Heum Kim(김창흠),Mi Ran Jeon(전미란),Eun Soo Seong(성은수),Yea Ji Lee(이예지),Jae Geun Lee(이재근),Chang Yeon Yu(유창연) 한국약용작물학회 2016 한국약용작물학회 학술대회논문집 Vol.2016 No.2
산삼배양근의 진세노사이드 증대를 위한 발효식품에서 분리 동정된 미생물들의 β-Glucosidase 활성
Byeong Ju Kang,Chul Joong Kim,Ji Hye Yoo,Mi Ran Jeon,Yea Ji Lee,Ki Hyun Kim,Jae Geun Lee,Eun Soo Seong,Chang Yeon Yu 한국약용작물학회 2016 한국약용작물학술대회 발표집 Vol.2016 No.05
Background : Ginsenosides, the main ingredient of ginseng roots can be confirmed various physiological activity such as anticancer, antioxidant, a natural ginsenosides is there a structure to be absorbed into the body does not work well absorbed through this process biologically active thus a high conversion ginsenosides. β-glucosidase enzyme is observed in several of the microorganism with an enzyme that serves to convert a ginsenoside prosper that is absorbed into the body. Methods and Results : To view a primary β-glucosidase activity, the bacteria were innoculated in esculin agar medium and the color change of the media were measured by the time and degree of changing color. In the other method, 5 mM of p-nitrophenyl-β-D-glucopyranoside (pNPG) containing 25 mM phosphate buffer solution (pH 7.0) was added to 50 ul enzyme solution. Then the solution was added to 50 ul reaction for 5 min at 30°C. The amount of p-nitrophenol liberated measured at 405 nm absorbance. The experimental results showed higher β-glucosidase activity in Pediococcus pentosaceus, Leuconostoc mesenteroide, Leuconostoc mesenteroides subsp. cremoris, and Paenibacillus polymyxa by using esculin agar medium method. Similarly in second method, β-glucosidase activity was higher in P. pentosaceus 402.32±11.43 unit/l, L. mesenteroide 353.73±14.64 unit/l, Lactobacillus sakei 198.4±15.47 unit/l Lactobacillus plantarum subsp. plantarum 164.1±8.12 unit/l. Conclusion : The result that the β-glucosidase activity was higher in P. pentosaceus, L. mesenteroide, and L. plantarum subsp. plantarum as compared to tested microbes. Therefore selected bacteria can be used in the industry of functioned foods and beverage to improve human healths.