http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Methamphetamine-Induced Neuronal Damage: Neurotoxicity and Neuroinflammation
( Buyun Kim ),( Jangmi Yun ),( Byoungduck Park ) 한국응용약물학회 2020 Biomolecules & Therapeutics(구 응용약물학회지) Vol.28 No.5
Methamphetamine (METH) is a highly addictive psychostimulant and one of the most widely abused drugs worldwide. The continuous use of METH eventually leads to drug addiction and causes serious health complications, including attention deficit, memory loss and cognitive decline. These neurological complications are strongly associated with METH-induced neurotoxicity and neuroinflammation, which leads to neuronal cell death. The current review investigates the molecular mechanisms underlying METH-mediated neuronal damages. Our analysis demonstrates that the process of neuronal impairment by METH is closely related to oxidative stress, transcription factor activation, DNA damage, excitatory toxicity and various apoptosis pathways. Thus, we reach the conclusion here that METH-induced neuronal damages are attributed to the neurotoxic and neuroinflammatory effect of the drug. This review provides an insight into the mechanisms of METH addiction and contributes to the discovery of therapeutic targets on neurological impairment by METH abuse.
( Buyun Kim ),( Sonam Jha ),( Ji Hae Seo ),( Chul-ho Jeong ),( Sooyeun Lee ),( Sangkil Lee ),( Young Ho Seo ),( Byoungduck Park ) 한국응용약물학회 2020 Biomolecules & Therapeutics(구 응용약물학회지) Vol.28 No.6
Methamphetamine (MA) is one of the most commonly abused drugs in the world by illegal drug users. Addiction to MA is a serious public health problem and effective therapies do not exist to date. It has also been reported that behavior induced by psychostimulants such as MA is related to histone deacetylase (HDAC). MeBib is an HDAC6 inhibitor derived from a benzimidazole scaffold. Many benzimidazole-containing compounds exhibit a wide range of pharmacological activity. In this study, we investigated whether HDAC6 inhibitor MeBib modulates the behavioral response in MA self-administered rats. Our results demonstrated that the number of active lever presses in MA self-administered rats was reduced by pretreatment with MeBib. In the hippocampus of rats, we also found MA administration promotes GluN2B, an NMDA receptor subunit, expression, which results in sequential activation of ERK/CREB/BDNF pathway, however, MeBib abrogated it. Collectively, we suggest that MeBib prevents the MA seeking response induced by MA administration and therefore, represents a potent candidate as an MA addiction inhibitor.
Buyun Liu,Hans-Joachim Lehmler,Yangbo Sun,Guifeng Xu,Qi Sun,Linda G. Snetselaar,Robert B. Wallace,Wei Bao 대한당뇨병학회 2019 Diabetes and Metabolism Journal Vol.43 No.1
Background: Bisphenol F (BPF) and bisphenol S (BPS) are increasingly used as substitutes for bisphenol A (BPA), an environmental obesogen. However, health effects of BPF and BPS remain unclear. In this study, we evaluated the associations of BPA, BPF, and BPS with obesity in children and adolescents. Methods: We used data from the U.S. National Health and Nutrition Examination Survey 2013 to 2014, a nationally representative study. We included 745 participants aged 6 to 17 years old. General obesity was defined based on the 2000 Centers for Disease Control and Prevention body mass index-for-age growth charts for the United States. Abdominal obesity was defined as waist-toheight ratio ≥0.5. Results: After adjustment for demographic, socioeconomic and lifestyle factors, and urinary creatinine levels, the odds ratio of general obesity comparing the highest with lowest quartile of urinary bisphenol levels was 1.74 (95% confidence interval [CI], 0.92 to 3.31) for BPA, 1.54 (95% CI, 1.02 to 2.32) for BPF, and 1.36 (95% CI, 0.53 to 3.51) for BPS. Moreover, the associations were stronger in boys than in girls for BPA and BPF. Similar results were observed for abdominal obesity. Conclusion: This study for the first time showed that exposure to BPF, a commonly used substitute for BPA, was positively associated with higher risk of obesity in children and adolescents. The association of BPA and BPF with general and abdominal obesity was primarily observed in boys, suggesting a possible sex difference. Further investigations on the underlying mechanisms are needed.
Buyun Jeong,Jinsung An,Kyoungphile Nam 대한환경공학회 2021 Environmental Engineering Research Vol.26 No.2
A site-specific, ecologically acceptable concentration of Cu in soil pore water was determined with four trophic levels of soil-residing organisms. Specifically, soil pore water was periodically collected from a site contaminated with heavy metals using in-situ samplers. Dissolved Cu concentration, Ca<SUP>2+</SUP>, Mg<SUP>2+</SUP>, Na<SUP>+</SUP>, K<SUP>+</SUP>, Cl<SUP>-</SUP>, SO₄<SUP>2-</SUP>, NO₃<SUP>-</SUP>, dissolved organic carbon, pH, and temperature were analyzed to derive a half-maximal effective concentration of Cu (EC50[Cu]T) using a biotic ligand model (BLM). The BLM parameters, such as binding constants (logKXBL) and the fraction of biotic ligand sites occupied by Cu ions (f), were adapted from previous studies. The EC50{Cu<SUP>2+</SUP>} values were used to construct a species sensitivity distribution (SSD) curve from which the hazardous concentration, protecting 95% of the soil-residing organisms (HC5), was determined. Using ten BLM-based acceptable concentrations of Cu obtained by combining BLM and SSD, time series analysis was conducted with the fixed monitoring benchmark method to obtain maximum Cu concentration as an endpoint exhibiting no-adverse-effect which was found to be 0.084 mg/L of Cu in soil pore water at the test site. This study provides a systematic tool for determining an ecologically acceptable concentration of Cu in the soil by incorporating soil pore water chemistry and time series analysis.
Kim, Buyun,Lee, Ki Yong,Park, Byoungduck Elsevier 2018 Phytomedicine Vol.51 No.-
<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Icariin is pharmacologically active prenylated flavonoid glycoside that has various biologic effects such as antioxidant, anticancer, and anti-inflammatory activities. In addition, icariin has been used in Chinese medicine for thousands of years to treat osteoporosis and it is still being used today. However, direct mechanism of icariin in the treatment of bone disease is not understood.</P> <P><B>Purpose</B></P> <P>The purpose of this study is to investigate whether icariin influences RANKL-induced osteoclast formation in murine macrophages.</P> <P><B>Methods</B></P> <P>Osteoclastogenesis was determined by TRAP staining and activity assay. Inhibition of signaling pathways and marker protein expression were evaluated by western blot analysis. The NF-κB (p65) nuclear localization was detected by immunofluorescence assay, and NF-κB/DNA-binding activity was detected by electrophoretic mobility shift assay (EMSA).</P> <P><B>Results</B></P> <P>In our study, icariin inhibited the differentiation of pre-osteoclast cells into osteoclasts and suppressed expression of various genes involved in osteoclast formation and bone resorption. Also, icariin blocked the osteoclastogenesis induced by MCF7 and MDA-MB-231 breast cancer cells through inhibition of NF-κB activation. We found that icariin inhibited RANKL-stimulated TRAF-6 expression, and subsequently suppressed the phosphorylation of ERK, but icariin did not show an effect on p38, JNK, and Akt activation.</P> <P><B>Conclusion</B></P> <P>These results indicate that icariin is likely to be a candidate for bone-related disease treatment and that icariin provides insights into the molecular mechanisms that influence RANKL-induced osteoclast differentiation.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Kim, Buyun,Yoon, Jaewoo,Yoon, Seong Woo,Park, Byoungduck SAGE Publications 2017 Integrative cancer therapies Vol.16 No.2
<P>Cysteine X cysteine (CXC) chemokine receptor 4 (CXCR4) and C-X-C motif chemokine 12 (CXCL12) were originally identified as chemoattractants between immune cells and sites of inflammation. Since studies have validated an increased level of CXCL12 and its receptor in patients with colorectal cancers, CXCL12/CXCR4 axis has been considered as a valuable marker of cancer metastasis. Therefore, identification of CXCR4 inhibitors has great potential to abrogate tumor metastasis. Onbaekwon (OBW) is a complex herbal formula that is derived from the literature of traditional Korean medicine <I>Dongeuibogam</I>. In this study, we demonstrated that OBW suppressed CXCR4 expression in various cancer cell types in a concentration- and time-dependent manner. Both proteasomal and lysosomal inhibitors had no effect to prevent the OBW-induced suppression of CXCR4, suggesting that the inhibitory effect of OBW was not due to proteolytic degradation but occurred at the transcriptional level. Electrophoretic mobility shift assay further confirmed that OBW could block endogenous activation of nuclear factor kappa B, a key transcription factor that regulates the expression of CXCR4 in colon cancer cells. Consistent with the aforementioned molecular basis, OBW abolished cell invasion induced by CXCL12 in colon cancer cells. Together, our results suggest that OBW, as a novel inhibitor of CXCR4, could be a promising therapeutic agent contributing to cancer treatment.</P>
A Criterion Describing the Dynamic Yield Behavior of PMMA
Ji Qiu,Tao Jin,Buyun Su,Qian Duan,Xuefeng Shu,Erqiang Liu,Xin Li 한국고분자학회 2019 Macromolecular Research Vol.27 No.8
Dynamic uniaxial and multiaxial tests were conducted to characterize the mechanical behavior of poly(methyl methacrylate) (PMMA). In this case, the multiaxial yield behavior of materials can be captured by the shear-compression samples (SCS). The experimental results were compared with different yield criteria, and which does not have a good agreement between theoretical prediction and experimental yield loci of PMMA undergoing combined shear-compression. Therefore, a phenomenological yield criterion is developed, and it notably involves hydrostatic stress sensitivity, lode angle, and one parameter. The parameter can be expressed by the asymmetry of yield strength between the tension and compression. The proposed criterion was verified by the utilization of differently combined shear-compression tests. The investigative results reveal that the proposed criterion can be successfully applied to describe the complex yield behavior of PMMA under dynamic loading conditions. The present study shows an efficient method to develop yield criterion of the reviewed materials.