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      • KCI등재

        Characterization of porcine cytokine inducible SH2-containing protein gene and its association with piglet diarrhea traits

        Buyue Niu,Dongchun Guo,Zhiran Liu,Xiaofei Han,Xibiao Wang 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.12

        Objective: The cytokine inducible SH2-containing protein (CISH), which might play a role in porcine intestine immune responses, was one of the promising candidate genes for piglet anti-disease traits. An experiment was conducted to characterize the porcine CISH (pCISH) gene and to evaluate its genetic effects on pig anti-disease breeding. Methods: Both reverse transcription polymerase chain reaction (RT-PCR) and PCR were performed to obtain the sequence of pCISH gene. A pEGFP-C1-CISH vector was constructed and transfected into PK-15 cells to analysis the distribution of pCISH. The sequences of individuals were compared with each other to find the polymorphisms in pCISH gene. The association analysis was performed in Min pigs and Landrace pigs to evaluate the genetic effects on piglet diarrhea traits. Results: In the present research, the coding sequence and genomic sequence of pCISH gene was obtained. Porcine CISH was mainly localized in cytoplasm. TaqI and HaeIII PCR restriction fragment length polymorphism (RFLP) assays were established to detect single nucleotide polymorphisms (SNPs); A-1575G in promoter region and A2497C in Intron1, respectively. Association studies indicated that SNP A-1575G was significantly associated with diarrhea index of Min piglets (p<0.05) and SNP A2497C was significantly associated with the diarrhea trait of both Min pig and Landrace piglets (p<0.05). Conclusion: This study suggested that the pCISH gene might be a novel candidate gene for pig anti-disease traits, and further studies are needed to confirm the results of this preliminary research.

      • 聂耳故乡高校红色文化育人创新路径探究

        靳明珠(Mingzhu Jin),张卜月(Buyue Zhang) YIXIN 출판사 2023 教育教学研究论丛 Vol.1 No.5

        随着互联网信息时代的爆炸式发展,处于成长发展期的“95 后”大学生极易被一些不良文化所误导,高校需要提升红色文化教育水平,避免大学生对红色文化不同程度的价值认同缺失。因此,高校教师在进行德育工作的时候,要将高校红色文化作为一种德育手段,利用当地红色资源对学生进行正确引导,从而提高大学生的思想政治教育效果。本文以聂耳故乡红色文化为切入点,对高校红色文化育人的内涵与必要性进行阐释,并以聂耳故乡红色文化的育人状况及面临的问题进行剖析,运用文献资料法、田野调研法、问卷调查法等多种研究手段,从价值导向效应、爱国课堂阵地建设、心理交流平台、拓展高校德育团队等方面探索聂耳家乡高校红色文化育人的创新途径。 With the explosive development of the Internet information age, the “post-1995” college students in the growth and development period are easily misled by some bad culture. Colleges and universities need to improve the level of red culture education to avoid college students’ lack of recognition of the value of red culture to different degrees.Therefore, when carrying out moral education, college teachers should take college red culture as a means of moral education and use local red resources to guide students correctly, so as to improve the effect of ideological and political education of college students.Taking the red culture in Nie Er’s hometown as the starting point, this paper gives a detailed definition of the connotation and importance of red culture education in colleges and universities, and analyzes the situation of red culture education in Nie Er ’s hometown and the problems it faces, using various research methods such as literature, field research and questionnaire. From the value-oriented effect, patriotic classroom position construction, psychological exchange platform, the expansion of moral education team in colleges and universities to explore the innovative way of red culture education in colleges and universities in Nie Er’s hometown.

      • KCI등재

        Experimental and Mechanical Behavior of Rubber-Sleeved Group Studs

        Yuxuan Chen,Quansheng Yan,Buyu Jia,Xiaolin Yu,Yu Wu,Yufan Luo 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.9

        The group studs used in precast steel-concrete composite bridges can reduce the volume of cast-in-place concrete, consequently accelerating the construction process and improving the construction quality. However, the small spacing of the studs in the group studs may result in a decrease in shear strength and ductility, and a nonuniform distribution of shear force at the root of the studs. Hence, rubber-sleeved group studs, whose studs are wrapped by a rubber sleeve at the root, are proposed to redistribute the shear force at the steel–concrete interface and improve the ductility of the shear connector. In this study, four push-out tests were conducted to demonstrate the composite mechanism of rubber-sleeved group studs. Results show that the rubber-sleeved group studs exhibit better ductility than ordinary group studs. Finite element models verified using the test results were also established for parametric analysis. The mechanism analysis shows that the concrete compression regions around stud roots are sufficiently utilized. Based on the parametric analysis, designing suggestions for rubber-sleeved group studs were provided.

      • KCI등재

        Experimental and Numerical Research on Uplift-Restricted and Slip-Permitted Screw-Shaped Connectors

        Yuxuan Chen,Quansheng Yan,Xiaolin Yu,Buyu Jia,Yu Wu,Yufan Luo 한국강구조학회 2022 International Journal of Steel Structures Vol.22 No.1

        Improving mechanical performance of the negative moment regions of continuous composite beams is essential for composite beam bridges. Uplift-restricted and slip-permitted (URSP) screw-shaped connectors can enhance the crack resistance of a concrete slab through free slip between the concrete slab and the steel beam. Furthermore, URSP screw-shaped connectors are more adaptable to the requirements of rapid construction than URSP T-shaped connectors. To investigate the slip and mechanical behavior of URSP screw-shaped connectors, nine push-out tests with various parameters were conducted. In these tests, the thickness of the foam sleeve wrapped outside the screw exerted a highly signifi cant eff ect on free slip performance. A refi ned 3D nonlinear fi nite element model with the foam’s constitutive model was established using ABAQUS. The stress mechanism and failure modes of the FE models were analyzed. Lastly, to estimate the slip performance of URSP screw-shaped connectors, an equation with a simple form was proposed via mechanism analysis and a regression method.

      • KCI등재

        A New Analytical Method for Link Slab Analysis

        Yufan Luo,Quansheng Yan,Xiaogang Yue,Buyu Jia,Xiaolin Yu,Yuxuan Chen 대한토목학회 2024 KSCE Journal of Civil Engineering Vol.28 No.2

        Link slabs have been widely used to reduce the number of expansion joints. However, traditional reinforced concrete link slabs are prone to cracking issues, making it crucial to accurately calculate their internal forces. Currently, the most advanced link slab analysis methods treat the link slab and adjacent span as a rotational spring to simulate their restriction on the girder end rotation. However, this method does not comprehensively consider the link slab's influence, leading to conservative results. To address this issue, this study further considers the influence of girder end deformations on adjacent span, resulting in more accurate calculations of the link slab's internal forces. Additionally, the concept of the girder end rotation reduction coefficient is put forward for the first time, which provides a clearer and more quantified understanding of the link slab's influence. The proposed method is verified by a model test and a site test, which shows a significant improvement in accuracy compared to existing analytical methods. On this basis, a parametric study is conducted to investigate the influence of various parameters on the girder end rotation reduction coefficient, which indicates that span length, girder spacing, and support configuration have the greatest influence.

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