HIGGS BOSON PRODUCTION UNDER THE RESONANCE THRESHOLD AT LEP II

Boos, E.,Dubinin, M.,Dudko, L. 서울대학교 이론물리학연구센터 1997 연구논문집 Vol.6 No.1

반란, 진보주의, 그리고 도덕 경제: 지난 20년의 아르헨티나 중산층

토비아스 부스(Tobias Boos),조영실(옮김) 서울대학교 라틴아메리카연구소 2021 라틴아메리카이슈 Vol.- No.-

The three-dimensional structure of TrmB, a transcriptional regulator of dual function in the hyperthermophilic archaeon Pyrococcus furiosus in complex with sucrose

Krug, M.,Lee, S.-J.,Boos, W.,Diederichs, K.,Welte, W. John Wiley Sons, Ltd 2013 Protein science Vol.22 No.6

Joint testing and profiling of microservice-based network services using TTCN-3

Manuel Peuster,Christian Dröge,Clemens Boos,Holger Karl 한국통신학회 2019 ICT Express Vol.5 No.2

The ongoing softwarization of networks creates a big need for automated testing solutions to ensure service quality. This becomes even more important if agile environments with short time to market and high demands, in terms of service performance and availability, are considered. In this paper, we introduce a novel testing solution for virtualized, microservice-based network functions and services, which we base on TTCN-3, a well known testing language defined by the European standards institute (ETSI). We use TTCN-3 not only for functional testing but also answer the question whether TTCN-3 can be used for performance profiling tasks as well. Finally, we demonstrate the proposed concepts and solutions in a case study using our open-source prototype to test and profile a chained network service.

The Interaction between Synoptic Scale Eddies and the East Asian Summer Monsoon

Hyo-Seok Park,Kyong-Hwan Seo,William Boos,Benjamin Lintner 한국기상학회 2015 한국기상학회 학술대회 논문집 Vol.2015 No.10

Characterization of the Transglycosylation Reaction of 4-α-Glucanotransferase (MalQ) and Its Role in Glycogen Breakdown in Escherichia coli

( Dang Hai Dang Nguyen ),( Sung-hoon Park ),( Phuong Lan Tran ),( Jung-wan Kim ),( Quang Tri Le ),( Winfried Boos ),( Jong-tae Park ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.3

We first confirmed the involvement of MalQ (4-α-glucanotransferase) in Escherichia coli glycogen breakdown by both in vitro and in vivo assays. In vivo tests of the knock-out mutant, ΔmalQ, showed that glycogen slowly decreased after the stationary phase compared to the wild-type strain, indicating the involvement of MalQ in glycogen degradation. In vitro assays incubated glycogen-mimic substrate, branched cyclodextrin (maltotetraosyl-β-CD: G4-β-CD) and glycogen phosphorylase (GlgP)-limit dextrin with a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ. In the absence of GlgP, the reaction of MalP, GlgX and MalQ on substrates produced glucose-1-P (glc-1-P) 3-fold faster than without MalQ. The results revealed that MalQ led to disproportionate G4 released from GlgP-limit dextrin to another acceptor, G4, which is phosphorylated by MalP. In contrast, in the absence of MalP, the reaction of GlgX, GlgP and MalQ resulted in a 1.6-fold increased production of glc-1-P than without MalQ. The result indicated that the G4-branch chains of GlgP-limit dextrin are released by GlgX hydrolysis, and then MalQ transfers the resultant G4 either to another branch chain or another G4 that can immediately be phosphorylated into glc-1-P by GlgP. Thus, we propose a model of two possible MalQ-involved pathways in glycogen degradation. The operon structure of MalP-defecting enterobacteria strongly supports the involvement of MalQ and GlgP as alternative pathways in glycogen degradation.

Structural rationale for the short branched substrate specificity of the glycogen debranching enzyme GlgX

Song, Hyung-Nam,Jung, Tae-Yang,Park, Jong-Tae,Park, Byung-Chul,Myung, Pyung Keun,Boos, Winfried,Woo, Eui-Jeon,Park, Kwan-Hwa Wiley Subscription Services, Inc., A Wiley Company 2010 Proteins Vol.78 No.8

<P>Glycogen serves as major energy storage in most living organisms. GlgX, with its gene in the glycogen degradation operon, functions in glycogen catabolism by selectively catalyzing the debranching of polysaccharide outer chains in bacterial glycosynthesis. GlgX hydrolyzes α-1,6-glycosidic linkages of phosphorylase-limit dextrin containing only three or four glucose subunits produced by glycogen phosphorylase. To understand its mechanism and unique substrate specificity toward short branched α-polyglucans, we determined the structure of GlgX from Escherichia Coli K12 at 2.25 Å resolution. The structure reveals a monomer consisting of three major domains with high structural similarity to the subunit of TreX, the oligomeric bifunctional glycogen debranching enzyme (GDE) from Sulfolobus. In the overlapping substrate binding groove, conserved residues Leu270, Asp271, and Pro208 block the cleft, yielding a shorter narrow GlgX cleft compared to that of TreX. Residues 207–213 form a unique helical conformation that is observed in both GlgX and TreX, possibly distinguishing GDEs from isoamylases and pullulanases. The structural feature observed at the substrate binding groove provides a molecular explanation for the unique substrate specificity of GlgX for G4 phosphorylase-limit dextrin and the discriminative activity of TreX and GlgX toward substrates of varying lengths. Proteins 2010. © 2010 Wiley-Liss, Inc.</P>

Role of Maltogenic Amylase and Pullulanase in Maltodextrin and Glycogen Metabolism of Bacillus subtilis 168

Shim, Jae-Hoon,Park, Jong-Tae,Hong, Jung-Sun,Kim, Ki Woo,Kim, Myo-Jeong,Auh, Jung-Hyuk,Kim, Young-Wan,Park, Cheon-Seok,Boos, Winfried,Kim, Jung-Wan,Park, Kwan-Hwa American Society for Microbiology 2009 Journal of Bacteriology Vol.191 No.15

<B>ABSTRACT</B><P>The physiological functions of two amylolytic enzymes, a maltogenic amylase (MAase) encoded by <I>yvdF</I> and a debranching enzyme (pullulanase) encoded by <I>amyX</I>, in the carbohydrate metabolism of <I>Bacillus subtilis</I> 168 were investigated using <I>yvdF</I>, <I>amyX</I>, and <I>yvdF amyX</I> mutant strains. An immunolocalization study revealed that YvdF was distributed on both sides of the cytoplasmic membrane and in the periplasm during vegetative growth but in the cytoplasm of prespores. Small carbohydrates such as maltoheptaose and β-cyclodextrin (β-CD) taken up by wild-type <I>B. subtilis</I> cells via two distinct transporters, the Mdx and Cyc ABC transporters, respectively, were hydrolyzed immediately to form smaller or linear maltodextrins. On the other hand, the <I>yvdF</I> mutant exhibited limited degradation of the substrates, indicating that, in the wild type, maltodextrins and β-CD were hydrolyzed by MAase while being taken up by the bacterium. With glycogen and branched β-CDs as substrates, pullulanase showed high-level specificity for the hydrolysis of the outer side chains of glycogen with three to five glucosyl residues. To investigate the roles of MAase and pullulanase in glycogen utilization, the following glycogen-overproducing strains were constructed: a <I>glg</I> mutant with a wild-type background, <I>yvdF glg</I> and <I>amyX glg</I> mutants, and a <I>glg</I> mutant with a double mutant (DM) background. The <I>amyX glg</I> and <I>glg</I> DM strains accumulated significantly larger amounts of glycogen than the <I>glg</I> mutant, while the <I>yvdF glg</I> strain accumulated an intermediate amount. Glycogen samples from the <I>amyX glg</I> and <I>glg</I> DM strains exhibited average molecular masses two and three times larger, respectively, than that of glycogen from the <I>glg</I> mutant. The results suggested that glycogen breakdown may be a sequential process that involves pullulanase and MAase, whereby pullulanase hydrolyzes the α-1,6-glycosidic linkage at the branch point to release a linear maltooligosaccharide that is then hydrolyzed into maltose and maltotriose by MAase.</P>

Role of Maltose Enzymes in Glycogen Synthesis by Escherichia coli

Park, J.-T.,Shim, J.-H.,Tran, P. L.,Hong, I.-H.,Yong, H.-U.,Oktavina, E. F.,Nguyen, H. D.,Kim, J.-W.,Lee, T. S.,Park, S.-H.,Boos, W.,Park, K.-H. American Society for Microbiology 2011 Journal of Bacteriology Vol.193 No.10

인터넷과 헌법상 쟁점들

李富夏 법무부 2004 선진상사법률연구 Vol.- No.22

Das Internet ist zur Zeit die weltweit größte und nach wie vor am schnellsten expandierende Kcmmunikationsstruktur. Electronic Mail (E-Mail) ist der am meisten genutzte Dienst im Internet E-Mail erlaubt es, Text von einem Computer auf einen anderen zu übertragen. Um E-Mail zu nutzen, benötigt man allerdings eine entsprechende Adresse. 1. Die Zusendung von unerwünschter E-Mail-Werbung an Private verstößt nach meiner Auffassung gegen Art 17 KVerfR, sofern der Empfänger nicht damit einverstanden ist oder sein Einverständnis auch nicht im Rahmen einer bereits bestehenden Geschäftsverbindung vermutet werden kann. Nach Auffassung der deutschen Gerichte ist das unaufgeforderte Zusenden von Spam-Mail wettbewerbsrechtlich unzulässig. Der Begriff von Spam-Mail ist mittlerweile ein fester Bestandteil jedes Internet-Glossars. Als Spam-Mail bezeichnet man das massenhafte Versenden von E-Mail im Internet Als verfassungswidrig gemäß Art 17 KVerfR (privacy) gilt die unaufgeforderte Bulk E-Mail beim Leeren des elektronischen Briefkastens die unverlangte Werbung unter Aufwand von Zeit, Mühe und Kosten aussortieren muss. 2. Pomographen, politisch Radikale, Rassisten, Militaristen, Antisemiten und Urheber beleidigender und verleumderischer Aussagen haben das Internet als weltweite unzensierte Plattform entdeckt Das ist nicht selten mit Verstößen gegen Rechtsnormen verbunden. Der Staat ist insbesondere immer dann verpflichtet schützend einzugreifen, wenn untragbare Folgen für individuelle und kollektive Rechtsgüter abzuwehren sind. Die Verbreitung verfassungsfeindlicher rassistischer Äußerungen, Gewaltaufrufe, nationalsozialistischen Gedankengutes und ähnlicher politradikaler Propaganda ist geeignet, die als fundamentale staatliche Gesamtordnung in den Grundwerten der Verfassung niedergelegte freiheitliche demokratische Grundordnung unmittelbar zu gefährden. Außerdem können rassistische, radikale Inhalte sowie Gewaltaufrufe die Würde des Menschen nach Art 10 KVerfR verletzen. 3. Fragliche ist, welche Bedeutung angesichts der zunehmenden Globalisierung dem Grundprinzip internationaler Zuständigkeit, dem Territorialprinzip, hinsichtlich nationaler Regelungen noch zukommen kann. Die Manifestation der Hoheitsakte in Form von schriftlichen Bescheiden oder Gewaltanwendungen läßt sich ebenfalls in den meisten Fällen ohne weiteres einem geographisch abgegrenzten Staatsgebiet zuordnen. Dem Territorialprinzip können die Staatsorgane einfach dadurch Genüge tun, indem sie auf dem Staatsgebiet verbleiben und physisch manifestierte Hoheitsakte nicht über die Grenzen hinweg gelangen lassen. Die Staaten dürfen aber über das Internet keine Hoheitsakte an Adressaten im Ausland versenden. Sie dürfen ebenfalls nicht über das Internet hoheitlich auf Inhalte zugreifen, die auf Rechnern im Ausland abgespeichert sind. 4. Betrachtet man die Kooperation und Koordination von Recht als vordringliche Aufgabe nicht nur auf der staatlichen, sondern auf der internationalen Ebene, so wird deutlich, dass dies ein umständliches, langwieriges und alles andere als einfaches Unterfangen ist. 인터넷의 보편화로 인한 발생하는 헌법상의 쟁점들이 계속하여 제기되고 있다. 특히 인터넷상의 가상공간에서의 표현은 다양하고 복잡한 헌법상의 문제를 불러 일으킨다. 첫째, 인터넷상 표현이 다른 매체에 의한 표현과 상이한 특성을 지니고 있다. 따라서 그에 대한 규율의 특이성이 요청된다. 특히 방송과의 차이를 고찰해보면, 인터넷상 표현은 신속성, 국제성, 비용저렴성, 대량정보전달 가능성의 특징을 지닌다. 그리고 가상공간에서의 ID의 사용은 무책임한 표현이나, 불건전한 정보의 유통을 가능케 한다는 문제점을 지니나, 그럼에도 불구하고 인터넷 가상공간에서의 익명의 사용은 헌법상 위헌의 소지가 없다. 왜냐하면 이러한 ID의 사용은 표현의 자유를 신장시킬 뿐만 아니라, ID사용으로 인하여 표현자(인터넷이용자)의 프라이버시권을 보호할 수 있기 때문이다. 인터넷사이트의 회원가입의 문제는 헌법상 양심의 자유와 인격권의 침해를 유발할 수 있다. 이는 특히 기본기입사항(필수사항)과 관련하여 문제된다. 인터넷상의 인종차별주의적, 폭력찬양적 정보, 폭력선동 및 명예훼손적 표현은 "자유민주적 기본질서"(우리헌법 제4조 참조)를 직접적으로 위태롭게 하며, 그밖에 우리 헌법 제10조에 의거한 "인간의 존엄과 가치"를 침해할 수 있다 둘째, 전통적인 주권개념에 의해 인터넷상의 표현들을 규제하는 주권적 행사를 발휘할 수 있는가는 헌법상 재정립해야 할 부분이다. 인터넷상의 불법적 표현을 규제하기 위해서는 국내법과 국제법의 공조가 필요하다. 셋째, 스팸메일은 규범상 "메일수신자의 의사에 반하여 보내진 전자메일"이라는 광의로 정의내리는 것이 타당하다. 스팸메일을 규제하는 방식으로는 옵트인(Opt-in)방식과 옵트아웃(Opt-out)방식이 있는데, 입법적으로는 온라인 서비스업체들 또는 스팸메일전송자가 스팸메일을 보내기 전에 수신이용자의 동의를 얻도록 하는 옵트인(Opt-in)방식이 실행되어야 할 것이다.