Network Analysis을 이용한 류마티스관절염 활액 대식세포에서 유전자 발현 연구

지종대 ( Jong Dae Ji ),김태환 ( Tae Hwan Kim ),이빛나라 ( Bit Na Ra Lee ),최성재 ( Sung Jae Choi ),이영호 ( Young Ho Lee ),송관규 ( Gwan Gyu Song ) 대한류마티스학회 2011 대한류마티스학회지 Vol.18 No.2

Objective. We wanted to investigate the mechanisms that could account for the pathogenesis of rheumatoid arthritis, so we examined the different expressions of the genes in rheumatoid arthritis (RA) synovial fluid macrophages as compared with that of normal peripheral blood (PB) monocyte-derived macrophages using microarray and bioinformatic analysis. Methods. We examined the expression of genes by using a gene expression oligonucleotide microarray. The differences of the gene expressions between the RA synovial macrophages and the normal PB monocytes-derived macrophages were analyzed using bioinformatic tools, including cytoscape and its plugin. Results. In this study, we found that 899 genes (464 genes up-regulated and 435 genes down-regulated) were differentially expressed between the two groups. Among the 899 genes, 552 genes were included for gene ontology analysis and network analysis. Based on biological process ontology, they were categorised mainly into immune response processes, responses to stimulus and signaling and regulation of biological processes. In addition to the genes related with STAT1 and AP-1 signaling, we found that the genes involved in the antigen processing and the cell cycle are abundantly expressed in RA synovial macrophages, suggesting that these genes may play an important role in the pathogenesis of RA. Conclusion. Our study suggest that this approach using integration of the gene expression profile with the protein interaction data may help to find several important pathogenic mechanisms in RA.

제5분과 : 환경관리 및 도시녹지 ; 옥상녹화 유형별 경관 변화 연구

이빛나라 ( Bit Na Ra Lee ),안근영 ( Geun Youmg Ahn ),이은희 ( Eun Hee Lee ) 한국환경생태학회 2013 한국환경생태학회 학술대회지 Vol.2013 No.1

현재 국내의 녹화시스템 유형은 토심, 식생종류, 경제성 등을 기준으로 일반적으로 저관리 경량형과 관리 중량형 그리고 혼합형으로 구분하고 있다. 하지만 이러한 녹화시스템 유형의 기준은 식생의 종류에 따른 관리요구도와 중량이 우선적으로 고려되었고, 녹화의 지속성 및 경관성에 대한 사항이 고려되지 않은 실정이다. 최근 들어 녹화시스템의 지속적인 유지가 가능한 식물소재 및 관리 기술개발로 옥상 녹화의 경관성 및 지속성을 확보할 수 있는 방안이 요구되고 있다. 이에 본 연구는 경관성 및 지속성 확보를 기초로 하는 새로운 녹화시스템의 유형 구분의 기초자료 제공을 목적으로 관리 정도의 차이가 시간변화에 따라 가시적 경관 변화에 미치는 영향 정도를 분석하였다. 가시적 경관변화 조사를 위하여, 설계유형, 식물수종의 보편성 및 다양성을 가지는 현장을 대상으로 서울 시내 녹화시스템 사례지 중 4개소(종로 헌법재판소, 강남구 강남구청, 광진구 상수도연구원, 동작구 서울시 녹지사업소)를 대상지로 선정하였다. 선정된 4개소의 대상지는 2012년 3월부터 10월 까지 매월 지점별 동일각도, 동일위치의 촬영지점을 다시 세부적으로 구분하여 가시적인 경관(녹시율) 시계열 변화정도를 모니터링 하였다. 조사는 실제 조성된 현장 사례지의 유형 파악을 통하여 관리자, 관수설비, 시비프로그램, 제초, 보식 등의 관리 강도에 따른 유형과 옥상녹화의 유형을 좌우하는 다양한 식물요소에 따른 유형으로 구분하여 진행하였다. 관리강도에 따른 유형의 경우 관리형과 저관리형으로 식물요소에 따른 유형으로는 초본(unit box, 포설형), 초본 + 관목, 초본 + 관목 + 교목으로 구분하여 가시적 경관변화를 조사하였다. 사진 촬영은 18~55mm 표준렌즈를 장착한 CANON 450D이 사용되었으며, 대표성 경관사진 전체 면적 중 살아있는 식물 잎의 영상이 차지하는 면적 비율(백분율)인 녹시율은 Auto CAD ver.2010 프로그램을 이용하여 산출하였다. 녹시율 분석 결과 전체적으로 모든 유형에서 관리가 계획적으로 이루어진 대상지의 녹시율이 높게 나타났고, 최소한의 관리 또는 관리가 이루어지지 않는 대상지 유형들은 녹시율이 상대적으로 낮게 나타났다. 식물의 생장 발육이 시작되는 3월의 경우 관리가 이루어지는 대상지의 식물군락의 생육활성이 더 활발한 것으로 분석되었으며, 4,5월의 경우 관리대상지의 녹시율 증가 속도가 더 빠른 것으로 조사되었다. 휴면기에 접어들기 시작하는 10월의 경우 관리가 이루어지는 대상지의 각 유형들이 더 오래 녹색을 유지하는 것으로 나타났다. 이는 열악한 옥상환경에서 식물의 생장, 생육과 관련된 기본적인 관리의 중요성을 증명하는 결과라 할수 있다. 유형간 녹시율의 변화를 비교하면, 초본류의 경우는 Unit box 유형에 비해 포설형의 녹시율이 더 양호한 것으로 나타났으며, 관리되고 있지 않은 대상지의 경우 녹시율에 포함되는 녹시량에 식재초종뿐만 아니라 이입종의 녹시량이 다분히 포함되고 있어 실제 계획된 식재초종의 녹시율로 한정 한다면 현재의 녹시율보다 현저히 떨어질 것으로 사료된다. 특히 포설형의 경우 저관리되고 있는 강남구청의 측정지점 녹시율이 7월에 폭증하고 있는데, 이는 한시적으로 증 가한 이입종에 의한 녹시량 증가로 판단되어 진다. 가시적 경관모니터링 결과, 고도의 관리가 아니더라도 정기적이고 체계적인 관리정도가 녹시율, 즉 식물의 생육활성화 정도에 영향을 주는 것을 확인 할 수 있었다. 경관모니터링 결과는 녹화시스템의 지속성 및 경관성 선별에 유용한 자료로 활용 가능 할 것이라 판단되며, 관리정도에 따른 녹화시스템의 새로운 유형 구분의 기초자료로 활용 될 것 이라 사료된다. 향후 환경의 변화가 다양한 인공지반녹지공간에서 경관 시계열 변화에 따른 녹화지의 환경기능성 모니터링을 통해 기후변화에 대응하는 도시녹화기술 기준정립에 관한 연구가 가능할 것이라 판단된다.

Evaluation of synbiotics as gut health improvement agents against Shiga toxin-producing Escherichia coli isolated from the pig

( Bo-ra Kim ),( Kyung Jin Cho ),( Doowan Kim ),( Jin Ho Cho ),( Jun Hyung Lee ),( Robin B. Guevarra ),( Sun Hee Lee ),( Jung Sun Kang ),( Won Tak Cho ),( Suphot Wattanaphansak ),( Bit Na Kang ),( Jong 한국축산학회(구 한국동물자원과학회) 2019 한국축산학회지 Vol.61 No.2

Colibacillosis is one of the major health problems in young piglets resulting in poor health and death caused by Escherichia coli producing F18 pili and Shiga toxin 2e. It is pivotal to reduce colibacillosis in weaned piglets to enhance production performance. In this study, we evaluated synbiotics as the gut health improvement agents in the mouse model challenged with Shiga toxin-producing E. coli (STEC) isolated from piglets. Prebiotic lactulose was formulated with each 5.0 × 10⁶ CFU/mL of Pediococcus acidilactici GB-U15, Lactobacillus plantarum GB-U17, and Lactobacillus plantarum GB 1-3 to produce 3 combinations of synbiotics. A total of 40 three weeks old BALB/c mice were randomly assigned to 4 groups (n = 10): a control group and 3 synbiotics treated groups. Each treatment groups were daily administrated with 5.0 × 10⁶ CFU/mL of one synbiotics for the first week, and every 3 days during the second week. All the mice were challenged with 8.0 × 10⁸ CFU/mL of STEC 5 days after animals began to receive synbiotics. Mice treated with synbiotics based on Pediococcus acidilactici GBU15 and Lactobacillus plantarum GB-U17 significantly improved daily weight gain compared to mice in other groups. While mice treated with GB-U15 showed better fecal index, no significant differences were observed among groups. Gross lesion and histopathological evaluations showed that mice treated with GB-U15 moderately improved recovery from STEC infection. In conclusion, our results suggest that the synbiotics formulated with lactulose and Pediococcus acidilactici GB-U15 have potential benefits to prevent and improve colibacillosis in weaned piglets.

제언 - 보다 나은 미래를 위한 선택, 산업안전보건

오빛나라,O, Bit-Na-Ra 대한산업보건협회 2020 산업보건 Vol.387 No.-

A Correlations of Experience of the Core Skill Education Program for the Fundamental of Nursing Skills, Self-Confidence for Clinical Performance and Nursing Academic Adjustment in Nursing Students

Ji Woo Kang,Bit Na Ra Kim,Mi Hye Seo 한국간호과학회 2015 한국간호과학회 학술대회 Vol.2015 No.10

Meta-Analysis of Effects on Comprehensive Sexuality Education Programs on Children and Adolescents

Eun-Joo Kim,Bit-Na Park,Seong-Kwang Kim,Min-Ju Park,Ju-Yeong Lee,A-Ra Jo,Min-Jae Kim,Ha-Neul Shin 한국간호과학회 2023 한국간호과학회 학술대회 Vol.2023 No.11

인간 대식세포에서 1,25(OH)2D3에 의한 CYP24 유전자 발현에 있어 MAPK의 역할

지종대 ( Jong Dae Ji ),이빛나라 ( Bit Na Ra Lee ),김태환 ( Tae Hwan Kim ),우진현 ( JIn Hyun Woo ),최성재 ( Sung Jae Choi ),이영호 ( Young Ho Lee ),송관규 ( Gwan Gyu Song ) 대한류마티스학회 2010 대한류마티스학회지 Vol.17 No.3

Objective: Several important roles of 1,25(OH)2D3 have been recognized in the immune system. The availability of 1,25(OH)2D3 at the cellular level is significantly influenced by the relative abundance of enzymes to synthesize (CYP27B1) and catabolize (CYP24) 1,25(OH)2D3. In this study, we examined the effect of 1,25(OH)2D3 on the expression of the CYP24 gene and the role of MAPK for the induction of CYP24 by 1,25(OH)2D3 in activated human macrophages. Methods: For obtaining human activated macrophages, we treated U937 cells with PMA and we cultured these cells for 24 hours to adhere. After 24 hours treatment with PMA, the differentiated cells were washed with phosphate buffered saline (PBS), and then they were used for examining the effect of 1,25(OH)2D3 on the expression of the CYP24 gene. The mRNA expressions of the vitamin-D3 inducible genes were measured by real-time PCR, and the change of the protein expression by 1,25(OH)2D3 was measured by immunoblotting. Results: 1,25(OH)2D3 significantly induced the expression of CYP24 in the U937 cells and the 1,25(OH)2D3-induced expression of CYP24 was strongly augmented in the PMA-differentiated U937 cells. The 1,25(OH)2D3-induced expression of CYP24 was mediated by Erk1/2 and p38 MAPKs. Parallel to the induced expression of CYP24, 1,25(OH)2D3 induced the expression and phosphorylation of the CCAAT enhancer-binding protein (C/EBPβ). Conclusion: In this study, we found that 1,25(OH)2D3 inducedthe expression of CYP24 via activation of MAPKs. These results suggest that MAPK inhibitors may be useful for the treatment of inflammatory conditions, in which active vitamin D3 can be used as the therapeutic molecule, by increasing the availability of 1,25(OH)2D3.

The dynamics of the piglet gut microbiome during the weaning transition in association with health and nutrition

Guevarra, Robin B.,Hong, Sang Hyun,Cho, Jin Ho,Kim, Bo-Ra,Shin, Jiwon,Lee, Jun Hyung,Kang, Bit Na,Kim, Young Hwa,Wattanaphansak, Suphot,Isaacson, Richard E.,Song, Minho,Kim, Hyeun Bum BioMed Central 2018 Journal of animal science and biotechnology Vol.9 No.-

<P><B>Background</B></P><P>Understanding the composition of the microbial community and its functional capacity during weaning is important for pig production as bacteria play important roles in the pig’s health and growth performance. However, limited information is available regarding the composition and function of the gut microbiome of piglets in early-life. Therefore, we performed 16S rRNA gene and whole metagenome shotgun sequencing of DNA from fecal samples from healthy piglets during weaning to measure microbiome shifts, and to identify the potential contribution of the early-life microbiota in shaping piglet health with a focus on microbial stress responses, carbohydrate and amino acid metabolism.</P><P><B>Results</B></P><P>The analysis of 16S rRNA genes and whole metagenome shotgun sequencing revealed significant compositional and functional differences between the fecal microbiome in nursing and weaned piglets. The fecal microbiome of the nursing piglets showed higher relative abundance of bacteria in the genus <I>Bacteroides</I> with abundant gene families related to the utilization of lactose and galactose. <I>Prevotella</I> and <I>Lactobacillus</I> were enriched in weaned piglets with an enrichment for the gene families associated with carbohydrate and amino acid metabolism. In addition, an analysis of the functional capacity of the fecal microbiome showed higher abundances of genes associated with heat shock and oxidative stress in the metagenome of weaned piglets compared to nursing piglets.</P><P><B>Conclusions</B></P><P>Overall, our data show that microbial shifts and changes in functional capacities of the piglet fecal microbiome resulted in potential reductions in the effects of stress, including dietary changes that occur during weaning. These results provide us with new insights into the piglet gut microbiome that contributes to the growth of the animal.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (10.1186/s40104-018-0269-6) contains supplementary material, which is available to authorized users.</P>

피페리딘 구조유도분자를 이용한 알루미노포스페이트 제올라이트 합성: 피페리딘 구조유도분자가 결정구조 형성에 미치는 영향

신혜선 ( Hye Sun Shin ),장익준 ( Ik Jun Jang ),신나라 ( Na Ra Shin ),주빛나 ( Bit Na Ju ),조성준 ( Sung June Cho ) 한국화학공학회 2011 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.49 No.5

Structure directing agent(SDA) containing piperidine moiety such as piperidine(PI), 2-methylpiperidine(MPI), 2,6-dimethylpiperidine(DMPI) and 2,2,6,6,-tetramethylpiperidine(TMPI), respectively has been utilized to synthesize aluminophosphate zeolite using hydrothermal method. The gel composition was (l.0)Al2O3:(1.0)P2O5:0.76SDA:(45)H2O and the hydrothermal heating was performed in an oven at 443 K and for 7 days at static mode. The obtained zeolitic material contained a lamellar structure when PI was used as the SDA. With a progressive increase of the SDA size, various structures of aluminophosphate including AlPO-5 of AFI structure were obtained. The aluminophosphate of SAS structure was formed when the largest TMPI was utilized as the SDA, which was confirmed by the Rietveld refinement. The result of 27 AI and 31P MAS NMR of the sample suggested that Al and P were incorporated into the framework of the aluminophosphate.

Regeneration of Completely Transected Spinal Cord Using Scaffold of Poly(D,L-Lactide-co-Glycolide)/Small Intestinal Submucosa Seeded with Rat Bone Marrow Stem Cells

Kang, Kkot Nim,Lee, Ju Young,Kim, Da Yeon,Lee, Bit Na,Ahn, Hyun Hee,Lee, Bong,Khang, Gilson,Park, So Ra,Min, Byoung Hyun,Kim, Jae Ho,Lee, Hai Bang,Kim, Moon Suk Mary Ann Liebert 2011 Tissue engineering. Part A Vol.17 No.17

<P>Using a complete spinal cord transection model, the present study employed a combinatorial strategy comprising rat bone marrow stem cells (rBMSCs) and polymer scaffolds to regenerate neurological function after spinal cord injury (SCI) of different lengths. SCI models with completely transected lesions were prepared by surgical removal of 1?mm (SC1) or 3?mm (SC3) lengths of spinal cord in the eighth-to-ninth spinal vertebrae, a procedure that resulted in bilateral hindlimb paralysis. A cylindrical poly(D,L-lactide-co-glycolide)/small intestinal submucosa scaffold 1 or 3?mm in length with or without rBMSCs was fitted into the completely transected lesion. Rats in SC1 and SC3 groups implanted with rBMSC-containing scaffolds received Basso-Beattie-Bresnahan scores for hindlimb locomotion of 15 and 8, respectively, compared with 3 for control rats in SC1-C and SC3-C groups implanted with scaffolds lacking rBMSCs. The amplitude of motor-evoked potentials recorded in the hindlimb area of the sensorimotor cortex after stimulation of the injured spinal cord averaged 100?μV in SC1-C and 10-50?μV in SC3-C groups at 4 weeks, and then declined to nearly zero at 8 weeks. In contrast, the amplitude of motor-evoked potentials increased from 300 to 350?μV between 4 and 8 weeks in SC1 rats and from 200 to 250?μV in SC3 rats. These results demonstrate functional recovery in rBMSC-transplanted rats, especially those with smaller defects. Immunohistochemically stained sections of the injury site showed clear evidence for axonal regeneration only in rBMSC-transplanted SC1 and SC3 models. In addition, rBMSCs were detected at the implanted site 4 and 8 weeks after transplantation, indicating cell survival in SCI. Collectively, our results indicate that therapeutic rBMSCs in a poly(D,L-lactide-co-glycolide)/small intestinal submucosa scaffold induced nerve regeneration in a complete spinal cord transection model and showed that functional recovery further depended on defect length.</P>