A Pilot Study on Screening of BRCA1 Mutations (185delAG, 1294del40) in Nepalese Breast Cancer Patients

Bhatta, Bibek,Thapa, Roshina,Shahi, Sanjay,Bhatta, Yogesh,Pandeya, Dipendra Raj,Poudel, Bal Hari Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.4

Background: Breast cancer is the second most common malignancy among Nepalese women, accounting for 60% of the total cancer cases in females. Women diagnosed with germline mutations in BRCA1 like 185delAG, 1294del40 develop breast and/or ovarian cancer with a lifelong likelihood of up to 85% whereas presence of a mutation increases the risk for mutations to occur in other genes. The major objective of this study was to find the prevalence of these mutations in Nepalese cancer patients. Materials and Methods: This prospective study was carried out at two cancer hospitals in the Kathmandu valley over a period of 11 months. Irrespective of age group and stage of canceran appropriate amount of blood was withdrawn from 50 breast cancer patients and 20 controls. DNA was extracted manually and subjected to PCR using primers for 185delAG and 1294del40 mutations. PCR products were then digested with restriction enzyme (DdeII) followed by electrophoresis. Results: Prevalence of 185delAG in reference breast cancer patients was found to be 4/50 (8%) but no 1294del40 was apparent. Conclusions: Several mutations occurring in different exons of BRCA1 as well as mutations in other genes like BRCA2, for example, should also be taken in account.

Studies on Feed Intake and Nutrient Utilization of Sheep under Two Housing Systems in a Semi-arid Region of India

Bhatta, Raghavendra,Swain, N.,Verma, D.L.,Singh, N.P. Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.6

An investigation was carried out to study the effect of two housing systems on feed intake and nutrient utilization of sheep in a semi-arid region of India. Two types of housing managements were adopted. The first was a shed- 20'${\times}$10' structure with all the four sides of 6' chain link fencing with central height of 10'. The roof was covered with asbestos sheets, with mud floorings. The second was an open corral- 20'${\times}$10' open space with all the four sides covered with 6' chain link fencing. Thirty-four (32 ewes and 2 rams) sheep were grazed together on a 35 ha plot of native range. All the sheep were grazed as a flock from 08:00 to 17:00 h during the yearlong study. The flock was divided into two groups (16 ewes+1 ram) in the evening and housed according to two housing systems (Shed and Open Corral). Three digestion trials were conducted during three defined seasons of monsoon, winter and summer seasons to determine the effect of housing on nutrient intake and utilization. Blood samples were collected in three seasons for the estimation of hemoglobin and glucose. Dry and wet bulb temperatures were recorded at 06:00 A.M. and 09:00 P.M. using suitable thermometers both inside the shed and in the open corral and temperature humidity index (THI) was calculated. There was significant (p<0.05) difference in the THI between shed and open corral in all the seasons, indicating that the shed was always warmer compared to open corral. The daily dry matter intake (DMI, g/d) was 965, 615 and 982 in sheep housed under shed and 971, 625 and 1,001 in those housed in open corral during monsoon, winter and summer season, respectively. These differences were however non-significant (p>0.05). The digestibility of DM was 45.92, 45.13 and 50.30 in sheep housed under shed and 43.64, 45.02 and 55.02 in sheep housed in open corral during monsoon, winter and summer seasons, respectively. There was no significant (p>0.05) difference in the digestibility of nutrients in sheep maintained under shed and in open corral. Blood Hb concentration was 13.97, 14.13 and 13.15 in sheep housed under shed and 15.27, 13.63 and 14.82 in those kept in open corral, whereas blood glucose concentration was 59.67, 59.70 and 52.33 in sheep under shed and 61.00, 61.00 and 57.83 in open corral, during monsoon, winter and summer, respectively. There was also no significant effect of housing on the body weight changes, wool yield and survivability in ewes. Although housing had no significant effect on nutrient intake, their utilization and blood parameters, there was significant effect on the physiological responses and energy expenditure of sheep maintained under the two housing systems (Bhatta et al., 2004). It can be concluded from this study that the housing systems didn't have any significant effect on the nutrient intake and utilization of native breed like Malpura, which were well adapted to the hot semi-arid conditions of India. However, while deciding provisions for housing of different breeds of sheep (both crossbred and native) parameters like physiological responses, energy expenditure, health conditions and overall economics of the systems should be taken into consideration.

The 72-h WEBT microvariability observation of blazar S5 0716 + 714 in 2009

Bhatta, G.,Webb, J. R.,Hollingsworth, H.,Dhalla, S.,Khanuja, A.,Bachev, R.,Blinov, D. A.,Bö,ttcher, M.,Bravo Calle, O. J. A.,Calcidese, P.,Capezzali, D.,Carosati, D.,Chigladze, R.,Collins, A.,Colo EDP Sciences 2013 Astronomy and astrophysics Vol.558 No.-

SMALL AMPLITUDE WAVE IN SHALLOW WATER OVER LINEAR AND QUADRATIC SLOPING BEDS

Bhatta, Dambaru D.,Debnath, Lokenath 한국전산응용수학회 2003 Journal of applied mathematics & informatics Vol.13 No.1

Here we present a study of small-amplitude, shallow water waves on sloping beds. The beds considered in this analysis are linear and quadratic in nature. First we start with stating the relevant governing equations and boundary conditions for the theory of water waves. Once the complete prescription of the water-wave problem is available based on some assumptions (like inviscid, irrotational flow), we normalize it by introducing a suitable set of non-dimensional variables and then we scale the variables with respect to the amplitude parameter. This helps us to characterize the various types of approximation. In the process, a summary of equations that represent different approximations of the water-wave problem is stated. All the relevant equations are presented in rectangular Cartesian coordinates. Then we derive the equations and boundary conditions for small-amplitude and shallow water waves. Two specific types of bed are considered for our calculations. One is a bed with constant slope and the other bed has a quadratic form of surface. These are solved by using separation of variables method.

NONLINEAR FREE SURFACE CONDITION DUE TO SECOND ORDER DIFFRACTION BY A PAIR OF CYLINDERS

BHATTA, DAMBARU D. 한국전산응용수학회 2005 Journal of applied mathematics & informatics Vol.18 No.1

An analysis of the non-homogeneous term involved in the free surface condition for second order wave diffraction on a pair of cylinders is presented. In the computations of the nonlinear loads on offshore structures, the most challenging task is the computation of the free surface integral. The main contribution to this integrand is due to the non-homogeneous term present in the free surface condition for second order scattered potential. In this paper, the free surface condition for the second order scattered potential is derived. Under the assumption of large spacing between the two cylinders, waves scattered by one cylinder may be replaced in the vicinity of the other cylinder by equivalent plane waves together with non-planner correction terms. Then solving a complex matrix equation, the first order scattered potential is derived and since the free surface term for second order scattered potential can be expressed in terms of the first order potentials, the free surface term can be obtained using the knowledge of first order potentials only.

Measurement of Methane Production from Ruminants

Bhatta, Raghavendra,Enishi, Osamu,Kurihara, Mitsunori Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.8

On a global scale agriculture and in particular enteric fermentation in ruminants is reported to produce about one fourth (21 to 25%) of the total anthropogenic emissions of methane ($CH_4$). Methane is produced during the anaerobic fermentation of hydrolyzed dietary carbohydrates in the rumen and represents an energy loss to the host besides contributing to emissions of greenhouse gases into the environment. However, there appears to be uncertainty in the $CH_4$ estimation from livestock due to the limited availability of data to document the variability at the farm level and also due to the significant impact of diet on the enteric $CH_4$ production. The methane mitigation strategies require robust prediction of emissions from rumen. There are many methods available which would be suitable for measuring $CH_4$ produced from the various stages of animal production. However, several factors need to be considered in order to select the most appropriate technique like the cost, level of accuracy required and the scale and design of the experiments to be undertaken. Selection of any technique depends on the accuracy as each one has its advantages and disadvantages. Screening of mitigation strategies may be evaluated using individual animal before large-scale trials on groups of animals are carried out. In this review various methods for the estimation of methane production from ruminants as well as for the determination of methane production potential of ruminant feeds are discussed. The advantages and disadvantages of the methods starting from respiration chamber, ventilated hood, facemask, sulphur hexafluoride ($SF_6$) tracer technique, prediction equations and meteorological methods to in vitro methods are detailed.

COMPUTATION OF ADDED MASS AND DAMPING COEFFICIENTS DUE TO A HEAVING CYLINDER

Bhatta, Dambaru D. 한국전산응용수학회 2007 Journal of applied mathematics & informatics Vol.23 No.1

We present the boundary value problem (BVP) for the heave motion due to a vertical circular cylinder in water of finite depth. The BVP is presented in terms of velocity potential function. The velocity potential is obtained by considering two regions, namely, interior region and exterior region. The solutions for these two regions are obtained by the method of separation of variables. The analytical expressions for the hydrodynamic coefficients are derived. Computational results are presented for various depth to radius and draft to radius ratios.

Phenolic Composition, Fermentation Profile, Protozoa Population and Methane Production from Sheanut (Butryospermum Parkii) Byproducts In vitro

Bhatta, Raghavendra,Mani, Saravanan,Baruah, Luna,Sampath, K.T. Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.10

Sheanut cake (SNC), expeller (SNE) and solvent extractions (SNSE) samples were evaluated to determine their suitability in animal feeding. The CP content was highest in SNSE (16.2%) followed by SNE (14.7%) and SNC (11.6%). However, metabolizable energy (ME, MJ/kg) was maximum in SNC (8.2) followed by SNE (7.9) and SNSE (7.0). The tannin phenol content was about 7.0 per cent and mostly in the form of hydrolyzable tannin (HT), whereas condensed tannin (CT) was less than one per cent. The in vitro gas production profiles indicated similar y max (maximum potential of gas production) among the 3 by-products. However, the rate of degradation (k) was maximum in SNC followed by SNE and SNSE. The $t^{1/2}$ (time taken for reaching half asymptote) was lowest in SNC (14.4 h) followed by SNE (18.7 h) and SNSE (21.9 h). The increment in the in vitro gas volume (ml/200 mg DM) with PEG (polyethylene glycol)-6000 (as a tannin binder) addition was 12.0 in SNC, 9.6 in SNE and 11.0 in SNSE, respectively. The highest ratio of $CH_4$ (ml) reduction per ml of the total gas, an indicator of the potential of tannin, was recorded in SNE (0.482) followed by SNC (0.301) and SNSE (0.261). There was significant (p<0.05) reduction in entodinia population and total protozoa population. Differential protozoa counts revealed that Entodinia populations increased to a greater extent than Holotricha when PEG was added. This is the first report on the antimethanogenic property of sheanut byproducts. It could be concluded that all the three forms of SN byproducts are medium source of protein and energy for ruminants. There is a great potential for SN by-products to be incorporated in ruminant feeding not only as a source of energy and protein, but also to protect the protein from rumen degradation and suppress enteric methanogenesis.

Comparison of In vivo and In vitro Techniques for Methane Production from Ruminant Diets

Bhatta, Raghavendra,Tajima, K.,Takusari, N.,Higuchi, K.,Enishi, O.,Kurihara, M. Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.7

This study was conducted to compare the methane ($CH_4$) production estimated by in vivo (sulfur hexafluoride tracer technique ($SF_6$)) with that of two in vitro rumen simulation (RUSITEC) and gas production (IVGPT)) techniques. Four adult dry Holstein cows, aged $7.4{\pm}3.0$ years and weighing $697{\pm}70$ kg, were used for measuring methane production from five diets by the $SF_6$ technique. The experimental diets were alfalfa hay ($D_1$), corn silage + soybean meal (SBM) (910: 90, $D_2$), Italian rye grass hay +SBM (920: 80, $D_3$), rice straw +SBM (910: 90, $D_4$) and Sudan grass hay +SBM (920: 80, $D_5$). Each diet was individually fed to all 4 cows and 5 feeding studies of 17 d each were conducted to measure the methane production. In the RUSITEC, methane production was measured from triplicate vessels for each diet .In vitro gas production was measured for each of the diets in triplicate syringes. The gas produced after 24 and 48 h was recorded and gas samples were collected in vacuum vials and the methane production was calculated after correction for standard temperature and pressure (STP). Compared to the $SF_6$ technique, estimates of methane production using the RUSITEC were lower for all diets. Methane production estimated from 24 h in vitro gas production was higher (p<0.001) on $D_1$ as compared to that measured by $SF_6$, whereas on $D_2$ to $D_5$ it was lower. Compared to $SF_6$, methane production estimated from 48 h in vitro gas production was higher on all diets. However, methane estimated from the mean of the two measurement intervals (24+48 h/2) in IVGPT was very close to that of $SF_6$ (correlation 0.98), except on $D_1$. The results of our study confirmed that IVGPT is reflective of in vivo conditions, so that it could be used to generate a database on methane production potential of various ruminant diets and to examine strategies to modify methane emissions by ruminants.

ADJOINT SYSTEM FOR A MAGNETO-CONVECTIVE FLOW IN AN ACTIVE MUSHY LAYER

Bhatta, Dambaru,Riahi, Daniel N. The Korean Society for Computational and Applied M 2011 Journal of applied mathematics & informatics Vol.29 No.5

Here we consider magneto-convection in a mushy layer which is formed during solidification of binary alloys. The mushy layer is treated as an active porous media with variable permeability. The equations governing the layer are conservation of mass, conservation of heat, conservation of solute, magnetic induction equation, momentum equation governed by the Darcy's law and Maxwell's equations for the magnetic field. To study the second order effects on the flow without solving the second order system, we need to obtain the adjoint system for the flow. This motivates the authors we derive the adjoint system analytically for the mushy layer case. Numerical results of the adjoint system are presented for passive and active mushy layers at the onset of the motion using a set of parameters experimentalists use.